Browse publications by year: 2024

  1. How SS, Nathan S, Lam SD, Chieng S
    J Zhejiang Univ Sci B, 2024 Oct 21;26(1):58-75.
    PMID: 39815611 DOI: 10.1631/jzus.B2300641
    Adenosine triphosphate (ATP)-binding cassette (ABC) transporter systems are divided into importers and exporters that facilitate the movement of diverse substrate molecules across the lipid bilayer, against the concentration gradient. These transporters comprise two highly conserved nucleotide-binding domains (NBDs) and two transmembrane domains (TMDs). Unlike ABC exporters, prokaryotic ABC importers require an additional substrate-binding protein (SBP) as a recognition site for specific substrate translocation. The discovery of a large number of ABC systems in bacterial pathogens revealed that these transporters are crucial for the establishment of bacterial infections. The existing literature has highlighted the roles of ABC transporters in bacterial growth, pathogenesis, and virulence. These roles include importing essential nutrients required for a variety of cellular processes and exporting outer membrane-associated virulence factors and antimicrobial substances. This review outlines the general structures and classification of ABC systems to provide a comprehensive view of the activities and roles of ABC transporters associated with bacterial virulence and pathogenesis during infection.
    MeSH terms: Adenosine Triphosphate/metabolism; Animals; Bacterial Infections/metabolism; Bacterial Infections/microbiology; Bacterial Proteins/metabolism; Humans; Virulence; Virulence Factors/metabolism
  2. Ting CH, Rahman MM, Chen YY, Safii R, Puteh SE, Saimon R, et al.
    Narra J, 2024 Dec;4(3):e1431.
    PMID: 39816059 DOI: 10.52225/narra.v4i3.1431
    Down syndrome is the most prevalent genetic condition contributing to intellectual disability. Advancements in medical care have significantly increased the life expectancy of people with this condition, making employment a vital component for independent living and quality of life. The aim of this study was to examine the current literature on the employability and employment experiences of individuals with Down syndrome, focusing on the evolution of the employment rate and factors influencing employment such as cognitive and personal factors, societal attitudes, challenges, and effective support systems. Using Arksey and O'Malley's methodology, a scoping review of relevant qualitative and quantitative articles from PubMed, Scopus, Web of Science (WoS), CINAHL, and Medline was conducted. The search focused on the keywords found in the title and abstract of articles from 1980 to 2023. The search strategy utilized medical subject headings (MeSH), including "work", "Down syndrome", and "employment". All articles employing qualitative, quantitative, and mixed methods that were published in English were included. Of an initial 4,296 articles, 32 full-text articles were evaluated, and nine met the inclusion criteria. The data indicated improved employment rates for people with Down syndrome, with a recent employment rate of 53%. However, jobs are often limited to sectors like food service and are underutilizing their skills. Key factors associated with employment status include cognitive abilities, family support, and social attitudes. Despite positive perceptions, significant barriers such as systemic bias persist. Effective support systems are crucial but are often hindered by limited opportunities. In conclusion, employment opportunities for people with Down syndrome have improved, but challenges such as limited job diversity and systemic barriers remain. Comprehensive policies are recommended to promote inclusive employment practices and tailored support systems.
    MeSH terms: Employment*; Humans; Quality of Life/psychology
  3. Puspitasari RN, I'tishom R, Kurnijasanti R, Mustafa MR, Sudjarwo SA
    Narra J, 2024 Dec;4(3):e1211.
    PMID: 39816095 DOI: 10.52225/narra.v4i3.1211
    Pancreatic cell damage in diabetes mellitus is closely linked to inflammation and apoptosis. This study aimed to investigate the protective effects of phloroglucinol on pancreatic cells in a streptozotocin-induced diabetic model by assessing its anti- inflammatory and anti-apoptotic mechanisms. Phloroglucinol ligand and the structures of Bax, Bcl-2, and caspase-3 proteins were sourced from the PubChem database. Molecular docking was performed using Autodock Tools and docking results were analyzed with PyRx software. In addition, during the in vivo study, the BALB/c mice were grouped into four categories: healthy control, untreated streptozotocin-induced diabetic, and streptozotocin-induced diabetic treated with two doses of oral phloroglucinol at 100 mg/kg and 200 mg/kg body weight. After 28 days, pancreatic tissues were collected for flow cytometric analysis of NF-κB, IL-6, TNF-α, and apoptotic markers (Bax, Bcl-2, and caspase-3). The docking simulations revealed specific binding interactions: phloroglucinol interacted with Bcl-2 via amino acid residues of ALA90 and TYR139, with Bax via ALA42, LEU45, ALA46, LEU47, PRO130, and ILE133, and with caspase-3 through ARG64, SER120, GLN161, CYS163, and ARG207. The binding affinities for Bax, Bcl-2, and caspase-3 were -5.0, -4.7, and -4.9 kcal/mol, respectively. In vivo, results showed that streptozotocin significantly elevated inflammatory cytokines NF-κB, TNF-α, and IL-6, along with apoptotic markers in pancreatic cells (p<0.05) compared to healthy controls. Phloroglucinol administration at 200 mg/kg significantly reduced TNF-α, NF-κB and IL- 6 levels. Phloroglucinol also prevented streptozotocin-induced pancreatic cell damage through anti-apoptotic effects by downregulating Bax and caspase-3 and upregulating Bcl-2. These findings suggest that phloroglucinol may offer protective benefits in diabetic conditions by modulating apoptotic and inflammatory pathways.
    MeSH terms: Animals; Male; Mice, Inbred BALB C*; Pancreas/drug effects; Pancreas/metabolism; Pancreas/pathology; Proto-Oncogene Proteins c-bcl-2/metabolism; bcl-2-Associated X Protein/metabolism; Mice; Caspase 3/metabolism; Molecular Docking Simulation*
  4. Tsaviv JN, Eneji IS, Sha'Ato R, Ahemen I, Jubu PR, Yusof Y
    Heliyon, 2024 Jul 30;10(14):e34517.
    PMID: 39816328 DOI: 10.1016/j.heliyon.2024.e34517
    The degradation of methylene blue dye-contaminated wastewater via photocatalysis is an efficient approach towards environmental remediation. The SrZrO3 perovskite photocatalyst was synthesized using the modified Pechini sol-gel method, and characterized using XRD, FESEM, FTIR, and UV-visible spectrophotometer. Crystallite size obtained by the Scherrer and Williamson-Hall methods were 45.56 and 44.50 nm, respectively. The sample exhibited an orthorhombic crystal structure. The optical bandgap was estimated to be 5.31 eV. Kinetic study for the degradation of methylene blue dye using SrZrO3 in the presence of H2O2 showed complete decontamination of the methylene blue dye in the time interval of 90-120 min under visible light irradiation. The degradation efficiency was above 80.1 % under illumination and less than 40.1 % in dark. Kinetic studies were performed by varying the dose of photocatalyst and initial concentration of methylene blue. It was observed that higher dose of the photocatalyst and lower concentration of the contaminant produced higher rate of degradation. The solution pH 3 and catalysts dosage of 200 mg yielded the highest rate of degradation. The experimental data fitted best into the Psuedosecond order kinetic model with the highest R2 value, indicating a strong linear relationship. The experimental data was subjected into nonlinear error functions, where the Psuedo-second order kinetic model demonstrated lower values of error functions. The results suggest that the prepared SrZrO3 photocatalyst coupled H2O2 is a promising photocatalyst for the decontamination of methylene blue dye under visible light irradiation.
  5. Han Q, Saad N, Md Isa K
    Heliyon, 2024 Jul 30;10(14):e34083.
    PMID: 39816343 DOI: 10.1016/j.heliyon.2024.e34083
    Road traffic injuries are one of the main causes of death among children. In recent years, the incidence and casualty rates of traffic accidents have increased year by year, which is a major challenge faced by safety organizations and governments in various countries, especially in developing countries. Among them, correct understanding of road traffic signs is a factor in reducing accidents. Therefore, identifying traffic signs has become an important indicator of road safety education. This study aimed to understand the understanding level of existing road traffic signs among Chinese children aged 7-11. 30 children aged 7-11 from Guangzhou participated in this study. The working title "Traffic Signage understanding Test questionnaire" was used as the tool for data collection. The questionnaire measured three aspects of child road users' understanding, including verbal label, action and consequences. Three experts in child psychology and cognition reviewed the questionnaire and validated its face and content. The survey results show that compared with actions and consequences, children have a lower understanding of verbal labels of signs, but overall, most children understand traffic signs incorrectly or do not understand them. This is statistically significant in reducing China's road traffic casualty rate. It is also recommended to design traffic signs that are suitable for children to understand.
  6. Kamarudin SS, Idris IB, Ahmad N, Sharip S
    Digit Health, 2024;10:20552076241292679.
    PMID: 39817043 DOI: 10.1177/20552076241292679
    BACKGROUND: Mental health care during the postpartum period is notably underexplored within Asian demographics, with barriers such as stigma, privacy concerns, logistical challenges, and a shortage of mental health professionals that limits access to optimal mental healthcare. Previous studies found that mobile health (mHealth) technology has been offering a promising solution to these issues. However, the perspectives of mothers on existing mental health services and their mHealth needs are still not well understood and warrant further exploration.

    OBJECTIVE: This study aimed to explore the postpartum experiences of Malaysian mothers with postpartum depression (PPD) symptoms and their perspectives on using an mHealth technology to improve mental health care during the postpartum period.

    METHODS: We carried out in-depth interviews with six mothers, which were transcribed verbatim and thematically analysed. Collaborative discussions and consensus were employed to enhanced reliability.

    FINDINGS: Five main themes related to mothers' postpartum experiences and perspectives emerged, namely, the existing circumstances of mothers, psychosociological reactions, maternal needs, innovation of care, and suggestions for pragmatic mHealth elements. These themes emphasized the emotional challenges of meeting societal, cultural, and personal expectations. The essential resources identified to be included in the mHealth technology were education on PPD, professional guided self-care, cognitive restructuring, validation of diverse infant feeding practices, and fostering partner support. Furthermore, interactive mHealth features such as mood trackers, notifications, platforms to share, and motivational tools aimed at elevating maternal well-being were also sought.

    CONCLUSIONS: Mothers expressed a desire for mHealth services emphasizing the need for these educational tools to be culturally sensitive, accommodating local language and religious practices, and offering support during confinement, to effectively address the unique needs of postpartum mothers. This study highlights the importance of prioritizing practical mHealth interventions as strategic support services to enhance positive postpartum mental care in resource-stricken countries.

  7. Shankar PR
    Indian J Med Ethics, 2024;IX(4):336-337.
    PMID: 39817297 DOI: 10.20529/IJME.2024.058
    I read with great interest the editorial on the pharmaceutical company-healthcare relationship published in the April-June issue of this journal [1]. Clinical practice guidelines are increasingly used by physicians to guide treatment decisions, and the pharmaceutical industry focuses on influencing the authors of these guidelines. Almost one in four guideline writers with no disclosed ties may have potentially relevant undisclosed ties to pharmaceutical companies [2]. Doctors are in a fiduciary relationship with patients and expected to act in their best interests. The relationship with the industry influences doctors in several ways; they may obtain information about medicines, but this information has been provided with an intention of influencing prescribing and increasing sales of the medicine [3].
    MeSH terms: Health Personnel/education; Humans; India; Physicians; Conflict of Interest*; Practice Guidelines as Topic; Disclosure
  8. Che Mohd Nassir CMN, Che Ramli MD, Jaffer U, Abdul Hamid H, Mehat MZ, Mohamad Ghazali M, et al.
    Curr Issues Mol Biol, 2024 Nov 28;46(12):13565-13582.
    PMID: 39727939 DOI: 10.3390/cimb46120811
    Coronavirus disease 2019 (COVID-19) has been a global pandemic affecting millions of people's lives, which has led to 'post-COVID-19 fatigue'. Alarmingly, severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) not only infects the lungs but also influences the heart and brain. Endothelial cell dysfunction and hypercoagulation, which we know occur with this infection, lead to thrombo-inflammation that can manifest as many myriad cardio-cerebrovascular disorders, such as brain fog, fatigue, cognitive dysfunction, etc. Additionally, SARS-CoV-2 has been associated with oxidative stress, protein aggregation, cytokine storm, and mitochondrial dysfunction in neurodegenerative diseases. Accordingly, the identification of molecular targets involved in these actions could provide strategies for preventing and treating this disease. In particular, the very common enzyme dipeptidyl peptidase IV (DPPIV) has recently been identified as a candidate co-receptor for the cell entry of the SARS-CoV-2 virus with its involvement in infection. In addition, DPPIV has been reported as a co-receptor for some viruses such as Middle East respiratory syndrome-coronavirus (MERS-CoV). It mediates immunologic reactions and diseases such as type 2 diabetes mellitus, obesity, and hypertension, which have been considered the prime risk factors for stroke among other types of cardio-cerebrovascular diseases. Unlike angiotensin-converting enzyme 2 (ACE2), DPPIV has been implicated in aggravating the course of infection due to its disruptive effect on inflammatory signaling networks and the neuro-glia-vascular unit. Regarding the neurological, physiological, and molecular grounds governing post-COVID-19 fatigue, this review focuses on DPPIV as one of such reasons that progressively establishes cerebrovascular grievances following SARS-CoV infection.
  9. Ahmad N B, Majed A AM, Syed A SS, Fadi G S, Irfhan A B HA, Salah A A, et al.
    Rev Invest Clin, 2024;76(6):262-273.
    PMID: 39809256 DOI: 10.24875/RIC.24000192
    Background: Limited information exists regarding the pathophysiological interactions between osteoporosis and chronic obstructive pulmonary disease (COPD). Objective: To study the association of Osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-Β ligand (RANKL) in male COPD patients. Methods: An observational clinical study was conducted at Penang General Hospital in Malaysia. Participants were divided into three groups: COPD patients with osteoporosis, COPD patients without osteoporosis, and healthy participants of the same age groups. Serum OPG (sOPG) and RANKL (sRANKL) levels were investigated. Results: The mean age of COPD patients was 64.10 ± 10.04 years. COPD patients had lower body mass index (23.22 ± 6.43) than healthy participants (27.32 ± 6.80). The T-score was significantly lower among COPD patients than healthy participants (p = 0.018). The sOPG concentration among healthy participants was significantly higher (361.90 ± 29.10 pg/mL, p < 0.001) than in the other groups, while the sRANKL concentration was not significantly different. The serum OPG/RANKL concentration was markedly higher in the control group than in the COPD patient group (p < 0.05). The COPD patients with osteoporosis had significantly lower pulmonary parameters (forced expiratory volume in the first [FEV]1% and FEV1/[forced vital capacity] (FVC), p < 0.01) and more dyspnea (modified medical research council = 2.60 ± 0.78 versus 1.90 ± 0.70, p < 0.01) than did the patients without osteoporosis. Furthermore, patients with severe COPD had a 3 times greater risk of developing osteoporosis (OR = 2.997 [95% CI = 2.181, 4.118], p < 0.001), while spirometric parameters had a significant inverse relationship with osteoporosis (FEV1% OR = 0.970, [95% CI = 0.954, 0.986], p = 0.001; FEV1/FVC OR = 0.984, (95% CI = 0.970, 0.999], p = 0.035). Conclusion: The study concluded that COPD patients had lower sOPG levels, leading to decreased OPG/RANKL ratio and faster bone resorption. Low bone mineral density was associated with more severe COPD. (Rev Invest Clin. 2024;76(6):262-73).
    MeSH terms: Aged; Cross-Sectional Studies; Humans; Malaysia/epidemiology; Male; Middle Aged; Body Mass Index; Case-Control Studies
  10. Khoo GGJ, Ong PACY, Wong JKW, Muhamad Yazid MAB, Chang CY
    Cureus, 2024 Dec;16(12):e75739.
    PMID: 39811204 DOI: 10.7759/cureus.75739
    Mycotic aneurysms are rare but severe complications that can arise from systemic bacterial infections, including those caused by Salmonella species. These aneurysms can progress rapidly and are associated with high mortality. A 62-year-old man with poorly controlled type 2 diabetes mellitus presented to the hospital in septic shock. The blood culture grew Salmonella enteritidis. A computed tomography angiogram showed a ruptured mycotic aneurysm of the infrarenal abdominal aorta. Despite antibiotic therapy and supportive care, the patient's condition rapidly deteriorated, resulting in his death, most likely due to aneurysm rupture. This case highlights the importance of vigilance in patients with Salmonella bacteremia, particularly in high-risk individuals, such as the elderly and people with underlying cardiovascular diseases. Early imaging and timely intervention are critical for improving outcomes and preventing fatal complications, such as aneurysm rupture.
  11. Mohammad NFA, Abdul Wahab NH, Kwang Sheng N, Ahmad Alwi AA, Muda R
    Cureus, 2024 Dec;16(12):e75715.
    PMID: 39811227 DOI: 10.7759/cureus.75715
    This is an unusual case of primary central nervous system lymphoma (PCNSL) with isolated third cranial nerve palsy as the initial manifestation. Neurolymphomatosis (NL) is a rare manifestation of PCNSL. While NL is a rare manifestation of PCNSL, primary vitreoretinal lymphoma (PVRL) can be the presenting feature or a later-involved manifestation. We present an unusual case of PCNSL with the isolated third cranial nerve palsy as the initial manifestation, which mimics the surgical third cranial nerve palsy. The patient developed PVRL subsequently and succumbed to the disease not long after the presentation.
  12. Wong WM, Tham YC, Ayton LN, Britten-Jones AC, Edwards TL, Grigg J, et al.
    Asia Pac J Ophthalmol (Phila), 2024;13(5):100098.
    PMID: 39366638 DOI: 10.1016/j.apjo.2024.100098
    PURPOSE: The objective of this paper is to shed light on the current landscape of genotyping practices, phenotyping practices and availability of essential vision rehabilitation management for inherited retinal diseases (IRD) in the Asia-Pacific (APAC) Region.

    METHODS: The 62-item questionnaire was distributed electronically via email. The questions covered five domains: (1) structure of the IRD service and registry/database; (2) genotyping practices; (3) genetic counselling; (4) deep phenotyping practices; (5) low-vision rehabilitation services.

    RESULTS: The survey was completed by 36 of 45 centres in twelve countries and regions in APAC. Among these centres, 42 % reported managing more than 1000 patients. Notably, 39 % of centres lack an IRD database or registry, and 44 % of centres have tested less than one-quarter of their IRD patients. The majority of centres (67 %) do not have genetic counsellors. While there was consistency in the imaging-based investigations, there was marked heterogeneity for functional testing using electrophysiology and formal perimetry. Only 34 % of centres confirmed the availability of access to low-vision assistive devices.

    CONCLUSIONS: This study reveals several critical gaps in managing IRDs in the APAC region. These include the lack of IRD database/registry in one-third of centres, a substantial proportion of patients remaining genetically undiagnosed, and limited availability of genetic counsellors. The findings also underscore a need to harmonise investigations for evaluating retinal function and identify areas for improvement in the provision of low-vision rehabilitation services.

    MeSH terms: Asia; Genetic Counseling; Humans; Phenotype; Practice Patterns, Physicians'/statistics & numerical data; Surveys and Questionnaires; Registries; Disease Management
  13. Lam J, El Nsouli D, Lee EL, Alqeisi T, Kane R, McGonagle I, et al.
    BMJ Open, 2024 Dec 23;14(12):e093636.
    PMID: 39719285 DOI: 10.1136/bmjopen-2024-093636
    OBJECTIVES: We sought to review studies that examine healthcare professionals' and students' knowledge, attitudes and practices (KAP) regarding medication disposal. We also explore recommendations and barriers related to appropriate medication disposal.

    DESIGN: A systematic review was conducted that adhered to Preferred Reporting Items for Systematic Reviews and Meta-Analyses.

    DATA SOURCES: MEDLINE, Embase, CINAHL, Web of Science, PsycINFO and Google Scholar were searched up to 23 February 2024.

    STUDY ELIGIBILITY CRITERIA AND SETTING: Qualitative, quantitative and mixed-method primary research studies. There was no limitation on the publication date, geographical locations or the study settings.

    PARTICIPANTS: Pharmacists, doctors, nurses and students from these respective professional groups in any country.

    PRIMARY OUTCOME MEASURES: The levels of healthcare staff and students' KAPs about disposal of unwanted medications.

    DATE EXTRACTION AND SYNTHESIS: Data extraction was conducted by four of the researchers independently. The study details were categorised into three main domains, that is, KAP using the KAP model. Other relevant information was also extracted, and synthesised in overall themes, such as challenges and recommendations.

    RESULTS: Thirty-seven studies from 18 countries (Asia n=21, the USA n=7, Africa n=5, EU n=2, South America n=2) were included. 86.5% (n=32) investigated participants' knowledge of medication disposal. Although there was a good level of awareness about the environmental impacts, there were significant gaps in knowledge regarding correct disposal methods, available services, guidelines and training. Thirty studies explored participants' attitudes towards medication disposal. There was a generally positive attitude towards the need for environmentally safe disposal practices. Thirty-five studies evaluated participants' practices in relation to medication disposal. Although there was generally a positive attitude and some understanding of appropriate disposal methods, the majority of the participants did not follow the practice guidelines, especially outside healthcare settings.

    DISCUSSIONS AND CONCLUSIONS: Although healthcare staff and students have fair knowledge and positive attitudes towards medicine disposal, their actual practices are lacking. One significant challenge identified is the limited awareness about proper disposal methods coupled with a lack of established services or guidelines. Even in cases where take-back programmes are available, they often face issues with accessibility. To tackle these challenges, it is suggested that governmental bodies should establish and enforce clear policies on medication disposal while also expanding educational initiatives to increase understanding among professionals and students. Furthermore, improving access to take-back programmes is crucial for ensuring safe medication disposal and minimising potential environmental and health hazards.

    PROSPERO REGISTRATION NUMBER: CRD42024503162.

    MeSH terms: Attitude of Health Personnel; Humans; Health Knowledge, Attitudes, Practice*; Medical Waste Disposal/methods; Medical Waste Disposal/standards
  14. Akhtar MT, Qadir R, Altaf U, Almas T, Batool S, Ikram MS, et al.
    Chem Biodivers, 2024 Dec 29.
    PMID: 39804576 DOI: 10.1002/cbdv.202402512
    The current study was conducted to characterize the vinegar extract of Nigella sativa and evaluate its biological activities using in vitro and in vivo studies. The N. sativa extract (NSE) was prepared by macerating seeds in a mixture of water and synthetic vinegar (1:10). The antioxidant potential of NSE was assessed, revealing high total phenolic contents (431.66 mg/100 g), total flavonoid contents (73.45 mg/100 g), total antioxidant capacity (118.55 mg/100 g), and strong radical scavenging potential (89.67%). The anticancer activity of NSE showed insignificant cytotoxic effects on HepG2 liver cell lines (96.61 ± 3.00). An in vivo animal trial was performed, and NSE significantly (p 
  15. Mohamad Lal MT, Seng Lim L, Lau LM, Long Chang ES, Momin AA, Izzan Hamid MS
    J Microorg Control, 2024;29(4):127-132.
    PMID: 39805610 DOI: 10.4265/jmc.29.4_127
    Marine fish farming served as a sustainable alternative to capture fisheries. However, it faced challenges such as disease management, water quality maintenance, and minimizing environmental impacts. Among these challenges, fungal infections are particularly concerning. Traditionally, chemical treatments raise concerns about potential toxicity to fish and humans, environmental impact, and resistance development. In response to these issues, there is growing interest in sustainable biocontrol strategies, including the use of probiotics, phytobiotics, postbiotics, microalgae, and fungal parasites as antifungal agents. This mini-review provides a brief overview of fungal infections in marine fish and discusses current fungal biocontrol strategies in aquaculture. By adopting these sustainable control methods, the aquaculture industry can improve fish health and productivity while reducing its reliance on harmful chemicals.
    MeSH terms: Fisheries; Fishes; Probiotics/administration & dosage; Phytochemicals/administration & dosage
  16. Zhao Y, Tu Y, Chew BH, Gacasan EM
    BMJ Open, 2024 Dec 20;14(12):e083018.
    PMID: 39806599 DOI: 10.1136/bmjopen-2023-083018
    INTRODUCTION: End-of-life care is essential for older adults aged ≥60, particularly those residing in long-term care facilities, such as nursing homes, which are known for their home-like environments compared with hospitals. Due to potential limitations in medical resources, collaboration with external healthcare providers is crucial to ensure comprehensive services within these settings. Previous studies have primarily focused on team-based models for end-of-life care in hospitals and home-based settings. However, there is a lack of sufficient evidence on practices in such facilities, particularly for Chinese older adults. The aim of this scoping review is to map the existing literature and inform the development of an appropriate care framework for end-of-life care in nursing homes. The focus of this article will be on the scope of services, guidelines for decision making, roles within interdisciplinary teams, and the practical feasibility of care provision.

    METHODS AND ANALYSIS: A systematic search will be conducted across nine electronic databases: PubMed, Scopus, EMBASE, Cochrane, PsycINFO, ERIC, CINAHL, China National Knowledge Infrastructure (CNKI), and Wanfang Data. The search will identify literature published in English and Chinese from January 2012 onwards. Articles will be selected based on their relevance to older adults aged ≥60 with disabilities or life-threatening chronic conditions receiving end-of-life care in nursing homes or similar settings. The data extraction process will be guided by the Canadian Hospice Palliative Care Association model (CHPCA) and the Respectful Death model. Qualitative data analysis will be performed using a framework method and thematic analysis, employing both inductive and deductive approaches, with three reviewers participating in the review process.

    ETHICS AND DISSEMINATION: Ethical approval is not required because the data for this review is obtained from selected publicly available articles. The results will be disseminated through publications in peer-reviewed journals and presented at relevant conferences. Furthermore, the findings will be shared with policymakers and healthcare professionals engaged in end-of-life care to inform practice and decision making.

    STUDY REGISTRATION: The review protocol has been registered on osf.io (https://osf.io/3u4mp).

    MeSH terms: Aged; China; Homes for the Aged/organization & administration; Homes for the Aged/standards; Humans; Nursing Homes*; Research Design; Review Literature as Topic; Terminal Care*
  17. Tan AH, Saffie-Awad P, Schumacher Schuh AF, Lim SY, Madoev H, Ahmad-Annuar A, et al.
    Neurol Genet, 2024 Dec;10(6):e200213.
    PMID: 39807215 DOI: 10.1212/NXG.0000000000200213
    BACKGROUND AND OBJECTIVES: In the era of precision medicine, genetic test results have become increasingly relevant in the care of patients with Parkinson disease (PD). While large research consortia are performing widespread research genetic testing to accelerate discoveries, debate continues about whether, and to what extent, the results should be returned to patients. Ethically, it is imperative to keep participants informed, especially when findings are potentially actionable. However, research testing may not hold the same standards required from clinical diagnostic laboratories and hold significant psychosocial implications. The absence of universally recognized protocols complicates the establishment of appropriate guidelines.

    METHODS: Aiming to develop recommendations on return of research results (RoR) practice within the Global Parkinson's Genetics Program (GP2), we conducted a global survey to gain insight on GP2 members' perceptions, practice, readiness, and needs surrounding RoR.

    RESULTS: GP2 members (n = 191), representing 147 institutions and 60 countries across 6 continents, completed the survey. Access to clinical genetic testing services was significantly higher in high-income countries compared with low- and middle-income countries (96.6% vs 58.4%), where funding was predominantly covered by patients themselves. While 92.7% of the respondents agreed that genetic research results should be returned, levels of agreement were higher for clinically relevant results relating to pathogenic or likely pathogenic variants in genes known to cause PD or other neurodegenerative diseases. Less than 10% offered separate clinically accredited genetic testing before returning genetic research results. A total of 48.7% reported having a specific statement on RoR policy in their ethics consent form, while 53.9% collected data on participants' preferences on RoR prospectively. 24.1% had formal genetic counselling training. Notably, the comfort level in returning incidental genetic findings or returning results to unaffected individuals remains low.

    DISCUSSION: Given the differences in resources and training for RoR, as well as ethical and regulatory considerations, tailored approaches are required to ensure equitable access to RoR. Several identified strategies to enhance RoR practices include improving informed consent processes, increasing capacity for genetic counselling including providing counselling toolkits for common genetic variants, broadening access to sustainable clinically accredited testing, building logistical infrastructure for RoR processes, and continuing public and health care education efforts on the important role of genetics in PD.

  18. Soo JQ, Wong XY, Chhabra N, Seow LL, Bhatia S
    J Dent Sci, 2024 Dec;19(Suppl 2):S143-S148.
    PMID: 39807254 DOI: 10.1016/j.jds.2024.07.026
    BACKGROUND/PURPOSE: Selecting an appropriate coronal restoration after root canal treatment requires careful consideration, however, there is a lack of established web-based tool that is based on clear guidelines for suitable post-endodontic restorations. Hence, we aimed to design and determine the acceptability of an interactive application to aid the decision making process for post-endodontic restoration among the undergraduate dental students.

    MATERIALS AND METHODS: A web-based post-endodontic restoration decision making interactive application, (RestoSmart) was developed after a detailed review of available literature. Seventy undergraduate dental students of IMU University were recruited in the study. A validated questionnaire was given to the participants before and after the use of RestoSmart to answer the provided mock clinical scenarios. Obtained data were statistically analyzed using IBM SPSS version 26 software package.

    RESULTS: The statistical analysis demonstrated that RestoSmart was acceptable among the undergraduate dental students (P 

  19. Zohdi RM, Adli MA, Mukhtar SM, Awang Junaidi AH, Bakar MZA
    J Taibah Univ Med Sci, 2024 Dec;19(6):1181-1192.
    PMID: 39807375 DOI: 10.1016/j.jtumed.2024.12.008
    OBJECTIVE: Concerns over the increasing number of obese individuals and the associated health risks have prompted therapeutic option explorations. Similarly, this study aimed to establish Sygyzium claviflorum fruit extract (SCFE) anti-adipogenic attributes in 3T3-L1 cells.

    METHODS: The polyphenolic compounds in SCFE were identified with Reverse phase-high performance liquid chromatography (RP-HPLC). Meanwhile, murine 3T3-L1 preadipocytes, measuring leptin levels, reactive oxygen species (ROS), and lipid and triglyceride (TG) contents were utilized during anti-adipogenic activity assessments. Concurrently, the effects of SCFE on adipogenic transcription factors were established with quantitative real-time-polymerase chain reaction (qRT-PCR).

    RESULTS: The RP-HPLC results indicated three polyphenolic compounds in SCFE, including one flavonoid (naringin) and two phenolic acids (syringic and p-coumaric). Although SCFE treatments (250-1000 μg/mL) did not result in cell toxicity, they significantly reduced dose-dependent lipid accumulation, ROS production, and TG and leptin levels relative to control-differentiated adipocytes. Moreover, SCFE suppressed sterol regulatory element binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor-gamma (PPAR-γ), and CCAAT/enhancer-binding protein-alpha (C/EBP-α) gene expressions during preadipocyte differentiation into adipocytes.

    CONCLUSION: The findings revealed the anti-adipogenic properties of SCFE, indicating its potential as a natural obesity management remedy. Nevertheless, more studies are necessary to elucidate the reactions resulting in SCFE anti-adipogenic effects and the active constituents responsible for the property.

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