Antinociceptive and antineuropathic effects of Trifolium resupinatum L. on formalin-induced nociception and cervical spinal cord hemi-contusion: Underlying Mechanisms

Jabbari S 1 , Zakaria ZA 2 , Mohammadi S 3

Affiliations 

  • 1 Department of Biology, Faculty of Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran. Electronic address: sajjadjabbarighorve@gmail.com
  • 2 Borneo Research on Algesia, Inflammation and Neurodegeneration (BRAIN) Group, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, 88400, Sabah, Malaysia; Department of Environmental Health, Faculty of Public Health, Campus C Universitas Airlangga, Jalan Mulyorejo, Surabaya, 60115 East Java, Indonesia. Electronic address: zaz@ums.edu.my
  • 3 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Electronic address: smiauhphd.sm@gmail.com
J Ethnopharmacol, 2025 Jan 30;337(Pt 2):118913.
PMID: 39369921 DOI: 10.1016/j.jep.2024.118913

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE: Trifolium resupinatum L. (Fabaceae), known as Persian clover, ethnomedicinally used in Persian folk medicine to treat peritoneal inflammation, rheumatism, and back pain.

AIM OF THE STUDY: To investigate the antineuropathic and antinociceptive activities of Trifolium resupinatum leaves essential oil (TREO) in male Wistar rats, as well as to explore the potential mechanisms of action.

MATERIALS AND METHODS: The antinociceptive activity of TREO and its main constituents, quercetin (Qc) was assessed using the formalin-induced paw licking test. Moreover, the potential mechanisms of antinociception were evaluated through various competitive and non-competitive antagonisms. Additionally, the antineuropathic potential was investigated using the cervical spinal cord hemi-contusion (CCS) model, and the role of phosphorylated Stat-3 was analyzed using Western blotting.

RESULTS: TREO exerted significant antinociceptive activity (P 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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