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Bioactivity profiling of Sanghuangporus lonicerinus: antioxidant, hypoglycaemic, and anticancer potential via in-vitro and in-silico approaches

Gafforov Y 1 , Bekić S 2 , Yarasheva M 3 , Mišković J 4 , Živanović N 2 , Chen JJ 5 Show all authors , Petri E 6 , Abdullaev B 1 , Rapior S 7 , Lim YW 8 , Abdullaev I 9 , Abbasi AM 10 , Ghosh S 11 , Wan-Mohtar WAAQI 12 , Rašeta M 2

Affiliations 

  • 1 Central Asian Center of Development Studies, New Uzbekistan University, Tashkent, Uzbekistan
  • 2 Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia
  • 3 Microbiology Laboratory, Navruz International Corp. LLC, Kibray, Uzbekistan
  • 4 Department of Biology and Ecology, Faculty of Sciences, ProFungi Laboratory, University of Novi Sad, Novi Sad, Serbia
  • 5 College of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang, China
  • 6 Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia
  • 7 CEFE, Univ Montpellier, CNRS, EPHE, IRD, Natural Substances and Chemical Mediation Team, Montpellier, France
  • 8 School of Biological Sciences and Institute of Biodiversity, Seoul National University, Seoul, Republic of Korea
  • 9 Khorezm Academy of Mamun, Khiva, Uzbekistan
  • 10 Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan
  • 11 Natural and Medical Sciences Research Centre, University of Nizwa, Nizwa, Oman
  • 12 Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia
J Enzyme Inhib Med Chem, 2025 Dec;40(1):2461185.
PMID: 39992291 DOI: 10.1080/14756366.2025.2461185

Abstract

This study investigates the mycochemical profile and biological activities of hydroethanolic (EtOH), chloroform (CHCl3), and hot water (H2O) extracts of Sanghuangporus lonicerinus from Uzbekistan. Antioxidant capacity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), NO, and FRAP assays, and in vitro hypoglycaemic effects were evaluated through α-amylase and α-glucosidase inhibition. Antiproliferative potential was explored by analysing the binding affinities of EtOH and H2O extracts to estrogen receptor α (ERα), ERβ, androgen receptor (AR), and glucocorticoid receptor (GR), with molecular docking providing structural insights. LC-MS/MS analysis revealed solvent-dependent phenolic profiles, with the EtOH extract containing the highest total phenolic content (143.15 ± 6.70 mg GAE/g d.w.) and the best antioxidant capacity. The EtOH extract showed significant hypoglycaemic effects, with 85.29 ± 5.58% inhibition of α-glucosidase and 41.21 ± 0.79% inhibition of α-amylase. Moderate ERβ binding suggests potential for estrogen-mediated cancer therapy, while strong AKR1C3 inhibition by the EtOH extract supports its therapeutic potential.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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