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Evaluation of polymerase chain reaction (PCR) method and hybrid capture II (HCII) assay for the detection of human papillomavirus in cervical scrapings

Saini R 1 , Shen TH 2 , Othman NH 2 , Santhanam J 3 , Othman N 2 , Tang TH 4

Affiliations 

  • 1 School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
  • 2 Department of Pathology, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
  • 3 Department of Biomedical Sciences, Institute of Allied Health Sciences, Universiti Kebangsaan Malaysia, ]alan Raja Muda Abdul Aziz, 50300 Kuala Lumpur
  • 4 Institute for Research in Molecular Medicine, (INFORMM), Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
Med J Malaysia, 2007 Aug;62(3):206-9.
PMID: 18246908 MyJurnal

Abstract

In order to investigate the reliability of detecting HPV DNA in cervical smears, we compared the performance of nested MY/GP PCR and FDA approved-Hybrid Capture II (HCII) using clinical cervical scrapings from 40 patients. It was found that PCR was more sensitive (81.8%) in comparison to HCII (36.4%) in detecting HPV although specificity of HCII was much higher (96.6%) than PCR (58.6%). The Negative Predictive Value (NPV) of both the techniques were quite similar but Positive Predictive Value (PPV) of HCII was much higher (80.0%) compared to PCR (42.9%). While the HCII method showed good specificity for HPV detection, its lack of sensitivity as compared to PCR may be a drawback for diagnostic use.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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