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Development and characterization of 27 microsatellite markers for the mangrove fern, Acrostichum aureum (Pteridaceae)

Yamamoto T 1 , Tsuda Y 2 , Mori GM 3 , Cruz MV 4 , Shinmura Y 5 , Wee AK 6 Show all authors , Takayama K 7 , Asakawa T 5 , Yamakawa T 5 , Suleiman M 8 , Núñez-Farfán J 9 , Webb EL 10 , Watano Y 5 , Kajita T 1

Affiliations 

  • 1 United Graduate School of Agricultural Science, Kagoshima University, 1-21-24 Korimoto, Kagoshima 980-0065, Japan; Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, 870 Uehara, Taketomi-cho, Yaeyama-gun, Okinawa 907-1541, Japan
  • 2 Sugadaira Montane Research Center, University of Tsukuba, 1278-294 Sugadairakogen, Ueda, Nagano 386-2204, Japan
  • 3 Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, 870 Uehara, Taketomi-cho, Yaeyama-gun, Okinawa 907-1541, Japan; Laboratório de Análise Genética e Molecular, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Brazil
  • 4 Laboratório de Análise Genética e Molecular, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Brazil
  • 5 Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi, Inage, Chiba, Chiba 263-8522, Japan
  • 6 College of Forestry, Guangxi University, Nanning, Guangxi, China
  • 7 Museum of Natural and Environmental History, Shizuoka, 5762 Oya, Suruga-ku, Shizuoka-shi, Shizuoka 422-8017, Japan
  • 8 Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Malaysia
  • 9 Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Apartado Postal 70-275, 04510 México D.F., México
  • 10 Department of Biological Sciences, National University of Singapore, Singapore, Singapore
Appl Plant Sci, 2016 Sep;4(9).
PMID: 27672519 DOI: 10.3732/apps.1600042

Abstract

PREMISE OF THE STUDY: Twenty-seven nuclear microsatellite markers were developed for the mangrove fern, Acrostichum aureum (Pteridaceae), to investigate the genetic structure and demographic history of the only pantropical mangrove plant.

METHODS AND RESULTS: Fifty-six A. aureum individuals from three populations were sampled and genotyped to characterize the 27 loci. The number of alleles and expected heterozygosity ranged from one to 15 and 0.000 to 0.893, respectively. Across the 26 polymorphic loci, the Malaysian population showed much higher levels of polymorphism compared to the other two populations in Guam and Brazil. Cross-amplification tests in the other two species from the genus determined that seven and six loci were amplifiable in A. danaeifolium and A. speciosum, respectively.

CONCLUSIONS: The 26 polymorphic microsatellite markers will be useful for future studies investigating the genetic structure and demographic history of of A. aureum, which has the widest distributional range of all mangrove plants.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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