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The complete methylome of Helicobacter pylori UM032

Lee WC 1 , Anton BP 2 , Wang S 3 , Baybayan P 4 , Singh S 5 , Ashby M 6 Show all authors , Chua EG 7 , Tay CY 8 , Thirriot F 9 , Loke MF 10 , Goh KL 11 , Marshall BJ 12 , Roberts RJ 13 , Vadivelu J 14

Affiliations 

  • 1 Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. woonching.lee@gmail.com
  • 2 New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA. anton@neb.com
  • 3 Pacific Biosciences, 1380 Willow Road, Menlo Park, CA, 94025, USA. swang@pacificbiosciences.com
  • 4 Pacific Biosciences, 1380 Willow Road, Menlo Park, CA, 94025, USA. pbaybayan@pacificbiosciences.com
  • 5 PacBio Singapore, Singapore, Singapore. ssingh@pacificbiosciences.com
  • 6 Pacific Biosciences, 1380 Willow Road, Menlo Park, CA, 94025, USA. mashby@pacbiosciences.com
  • 7 Marshall Centre, School of Pathology & Laboratory Medicine, The University of Western Australia, 6009, Perth, Australia. eng.chua@uwa.edu.au
  • 8 Marshall Centre, School of Pathology & Laboratory Medicine, The University of Western Australia, 6009, Perth, Australia. alfred.tay@uwa.edu.au
  • 9 Marshall Centre, School of Pathology & Laboratory Medicine, The University of Western Australia, 6009, Perth, Australia. fanny.thirriot@uwa.edu.au
  • 10 Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. lokemunfai@um.edu.my
  • 11 Department of Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. gohkl@um.edu.my
  • 12 Marshall Centre, School of Pathology & Laboratory Medicine, The University of Western Australia, 6009, Perth, Australia. barry.marshall@uwa.edu.au
  • 13 New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA. roberts@neb.com
  • 14 Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. jamuna@um.edu.my
BMC Genomics, 2015;16:424.
PMID: 26031894 DOI: 10.1186/s12864-015-1585-2

Abstract

The genome of the human gastric pathogen Helicobacter pylori encodes a large number of DNA methyltransferases (MTases), some of which are shared among many strains, and others of which are unique to a given strain. The MTases have potential roles in the survival of the bacterium. In this study, we sequenced a Malaysian H. pylori clinical strain, designated UM032, by using a combination of PacBio Single Molecule, Real-Time (SMRT) and Illumina MiSeq next generation sequencing platforms, and used the SMRT data to characterize the set of methylated bases (the methylome).

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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