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A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues

Higuchi A 1 , Wang CT 2 , Ling QD 3 , Lee HH 4 , Kumar SS 5 , Chang Y 6 Show all authors , Alarfaj AA 7 , Munusamy MA 7 , Hsu ST 8 , Wu GJ 9 , Umezawa A 10

Affiliations 

  • 1 1] Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan, Taiwan [2] Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia [3] Department of Reproduction, National Research Institute for Child Health and Development, Tokyo, Japan [4] Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan
  • 2 Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan, Taiwan
  • 3 1] Institute of Systems Biology and Bioinformatics, National Central University, Jhong-li, Taoyuan, Taiwan [2] Cathay Medical Research Institute, Cathay General Hospital, Taipei, Taiwan
  • 4 Department of Surgery, Cathay General Hospital, Taipei, Taiwan
  • 5 Department of Medical Microbiology and Parasitology, Universities Putra Malaysia, Slangor, Malaysia
  • 6 Department of Chemical Engineering, R&D Center for Membrane Technology, Chung Yuan Christian University, Jhong-li, Taoyuan, Taiwan
  • 7 Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia
  • 8 Department of Internal Medicine, Taiwan Landseed Hospital, Pingjen, Taoyuan, Taiwan
  • 9 Graduate Institute of Medical Sciences and Department of Obstetrics &Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
  • 10 Department of Reproduction, National Research Institute for Child Health and Development, Tokyo, Japan
Sci Rep, 2015;5:10217.
PMID: 25970301 DOI: 10.1038/srep10217

Abstract

Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 μm, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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