Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment

Bindal P 1 , Gnanasegaran N 2 , Bindal U 3 , Haque N 4 , Ramasamy TS 5 , Chai WL 2 Show all authors , Kasim NHA 2

Affiliations 

  • 1 Centre for Rural Health, School of Health Sciences University of Tasmania, Newnham, Launceston, 7250, Australia. priyagogia78@yahoo.com
  • 2 Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia
  • 3 Faculty of Health and Medical Sciences, Taylor's University, Subang Jaya, Malaysia
  • 4 Faculty of Dentistry, MAHSA University, Jenjarom, Malaysia
  • 5 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia
Clin Oral Investig, 2019 Oct;23(10):3821-3831.
PMID: 30687907 DOI: 10.1007/s00784-019-02811-5

Abstract

OBJECTIVE: In this study, we aimed to determine the suitable concentrations of human platelet lysate (HPL) and platelet-rich plasma (PRP) for maintaining the in vitro proliferative and angiogenic potential of inflamed dental pulp stem cells.

MATERIALS AND METHODS: Lipopolysaccharide (LPS)-induced inflamed dental pulp-derived stem cells (iDPSCs) were treated with different concentrations of HPL and PRP (10% and 20%) followed by determination of viability using Alamar Blue assay. Expression of angiogenesis-, adhesion-, and inflammation-regulating genes was also analyzed using RT-qPCR array. Furthermore, expression of growth factors at protein level in the cell culture microenvironment was measured using multiplex assay.

RESULTS: Viability of iDPSCs was significantly (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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