Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Arora S 1 , Steuernagel B 1 , Gaurav K 1 , Chandramohan S 2 , Long Y 3 , Matny O 4 Show all authors , Johnson R 4 , Enk J 5 , Periyannan S 2 , Singh N 6 , Asyraf Md Hatta M 1 , Athiyannan N 2 , Cheema J 1 , Yu G 1 , Kangara N 1 , Ghosh S 1 , Szabo LJ 7 , Poland J 6 , Bariana H 8 , Jones JDG 9 , Bentley AR 10 , Ayliffe M 2 , Olson E 11 , Xu SS 12 , Steffenson BJ 4 , Lagudah E 2 , Wulff BBH 13

Affiliations 

  • 1 John Innes Centre, Norwich Research Park, Norwich, UK
  • 2 Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, Australian Capital Territory, Australia
  • 3 Department of Plant Sciences, North Dakota State University, Fargo, ND, USA
  • 4 Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA
  • 5 Arbor Biosciences, Ann Arbor, MI, USA
  • 6 Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, USA
  • 7 US Department of Agriculture, Agriculture Research Service, Cereal Disease Laboratory, St. Paul, MN, USA
  • 8 The University of Sydney Plant Breeding Institute, Cobbitty, New South Wales, Australia
  • 9 The Sainsbury Laboratory, Norwich Research Park, Norwich, UK
  • 10 The John Bingham Laboratory, NIAB, Cambridge, UK
  • 11 Michigan State University, East Lansing, MI, USA
  • 12 US Department of Agriculture, Agriculture Research Service, Northern Crop Science Laboratory, Cereal Crops Research Unit, Red River Valley Agricultural Research Center, Fargo, ND, USA
  • 13 John Innes Centre, Norwich Research Park, Norwich, UK. brande.wulff@jic.ac.uk
Nat Biotechnol, 2019 02;37(2):139-143.
PMID: 30718880 DOI: 10.1038/s41587-018-0007-9

Abstract

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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