Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand

Hempolchom C 1 , Reamtong O 2 , Sookrung N 3 , Srisuka W 4 , Sakolvaree Y 5 , Chaicumpa W 6 Show all authors , Taai K 7 , Dedkhad W 8 , Jariyapan N 9 , Takaoka H 10 , Saeung A 11

Affiliations 

  • 1 Graduate PhD's Degree Program in Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: chayanit.hem@gmail.com
  • 2 Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. Electronic address: onrapak.rea@mahidol.edu
  • 3 Biomedical Research Incubator Unit, Department of Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Electronic address: nitat.soo@gmail.com
  • 4 Entomology Section, Queen Sirikit Botanic Garden, Chiang Mai, 50180, Thailand. Electronic address: wsrisuka@gmail.com
  • 5 Center of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Electronic address: jub922@yahoo.com
  • 6 Center of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Electronic address: wanpen.cha@mahidol.ac.th
  • 7 Faculty of Veterinary Medicine, Western University, Kanchanaburi 71170, Thailand. Electronic address: kritsana_taai@hotmail.com
  • 8 Graduate PhD's Degree Program in Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: wd.dedkhad@gmail.com
  • 9 Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: njariyapan@gmail.com
  • 10 Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand; Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Kuala Lumpur 50603, Malaysia. Electronic address: takaoka@oita-u.ac.jp
  • 11 Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: atisaeung.noi@gmail.com
Acta Trop, 2019 Jun;194:82-88.
PMID: 30922801 DOI: 10.1016/j.actatropica.2019.03.026

Abstract

Although several studies have reported pharmacological and immunological activity, as well as the role of black flies in transmitting pathogens to vertebrate hosts through salivary glands (SG) during blood feeding, SG proteomes of the anthropophilic black flies in Thailand have never been reported. Therefore, this study determined the SG proteomes of female S. nigrogilvum and S. nodosum. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional (2-DE) gels containing separated SG proteins of individual species were subjected to liquid chromatography-tandem mass spectrometry (LCMS/MS) and an orthologous protein search from eukaryotic organism, nematocera and simuliidae databases for total protein identification. SDS-PAGE and protein staining revealed at least 13 and 9 major protein bands in the SGs of female S. nigrogilvum and S. nodosum, respectively, as well as several minor ones. The 2-DE demonstrated a total of 56 and 41 protein spots for S. nigrogilvum and S. nodosum, respectively. Most of the proteins obtained in both species were enzymes involved in blood feeding, including proteases, apyrases, hyaluronidases, aminopeptidase and elastase. The results obtained in this study provided a new body of knowledge for a better understanding on the role of salivary gland proteins in these black fly species in Thailand.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications