Introduction:HPV vaccination is considered to be the primary form of cervical cancer prevention. However, the practice of HPV vaccination in Malaysia was not satisfied based on low vaccination rate among Malaysian. The health personal play a major roles in this scenario hence; this study was conducted to determine the knowledge and awareness on human papillomavirus vaccination and its relationship with socio –demographic among final year nursing and medical students in University Kebangsaan Malaysia Medical Center. Methods: A cross-sectional design was conducted in the faculty of medicine, University Kebangsaan Malaysia Medical Center. Two hundred and thirty four students were recruited using universal sampling equivalent to 86% respondent rate. Data was collected using self-administered structured questionnaires contains three sections. Pearson Chi-square analyses were used to de-termine the relationship between various socio-demographic factors and outcome variables namely knowledge and awareness on HPV vaccination. Results: Findings indicates that most of the respondents (85.9%) had a high level knowledge and (100%) awareness on HPV vaccination. Among the socio-demographic factors, respondents’ race (p=0.006) and marital status (p=0.0006) were associated with knowledge while, there were significant relationship between awareness and gender (p=0.0001). Conclusion: It can be concluded, that final year nursing and medical students had high knowledge and awareness on HPV vaccination. Race and marital status affected the knowledge while gender and family history of cervical cancer are significant predictors for awareness on HPV vaccination.