Introduction: Nutrition education is crucial to promote healthy eating behaviours and lifestyle that can prevent nutrition related non-communicable diseases. Young children are known to be amenable to nutrition education programmes and the school setting provides ideal opportunity for young children to learn. It is important to measure the impact of such programmes to determine its usefulness. Thus, the current study described the impact of a short-term nutrition education programme in kindergarteners. Methods: This pre and post-test design study involved 20 kindergarteners (aged 6 years) at Pusat Minda Lestari, Universiti Malaysia Sabah. The participants of the programme received a nutrition education module at school which comprised of nutrition lessons on key messages in the Ma-laysian Dietary Guidelines and related hands-on activities for 30min/day on 10 consecutive school days. Main outcomes were children’s nutrition knowledge and attitude, their preference for fruits and/or vegetables in a meal and types of physical activity most frequently done. These were all measured using established age-appropriate methods and tools. Results: After receiving the nutrition module; study participants’ mean score for overall nutrition knowledge significantly improved by 60%, self-reported attitude towards healthy foods were more positive and the majority of participants (85% combined) reported that they would prefer to include either vegetables, fruits or vege-tables & fruits in their meal rather than none at all (65% preferred no fruits and vegetables at baseline). The number of participants who said they preferred doing healthier types of physical activities rather than sedentary activities increased by 40% after the programme. Conclusion: This short-term nutrition programme appeared to increase kin-dergarteners’ interest in making healthy food choices and doing moderate to high intensity physical activities. Thus, the nutrition education module used in this programme may be useful in promoting healthy eating behaviours and physical activity in young children.