Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation

Mehta M 1 , Paudel KR 2 , Shukla SD 3 , Shastri MD 4 , Satija S 5 , Singh SK 5 Show all authors , Gulati M 5 , Dureja H 6 , Zacconi FC 7 , Hansbro PM 2 , Chellappan DK 8 , Dua K 1

Affiliations 

  • 1 Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, NSW 2007, Australia
  • 2 Centre for Inflammation, Centenary Institute, Sydney, NSW 2050, Australia
  • 3 Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI), University of Newcastle, New Lambton Heights, Newcastle, NSW 2305, Australia
  • 4 School of Health Sciences, College of Health & Medicine, University of Tasmania, Launceston, TAS 7250, Australia
  • 5 School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
  • 6 Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana 124001, India
  • 7 Departamento de Química Orgánica, Facultad de Química y de Farma-cia, Pontificia Universidad Católica deChile, Av. Vicuña Mackenna 4860, Macul, Santiago 7820436, Chile
  • 8 Department of Life Sciences, School of Pharmacy, International Medical University (IMU), Bukit Jalil 57000, Kuala Lumpur, Malaysia
Future Med Chem, 2021 03;13(6):543-549.
PMID: 33538615 DOI: 10.4155/fmc-2020-0297

Abstract

Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); γ-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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