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Zerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro

Paudel KR 1 , Clarence DD 2 , Panth N 1 , Manandhar B 3 , De Rubis G 3 , Devkota HP 4 Show all authors , Gupta G 5 , Zacconi FC 6 , Williams KA 3 , Pont LG 3 , Singh SK 7 , Warkiani ME 8 , Adams J 7 , MacLoughlin R 9 , Oliver BG 10 , Chellappan DK 11 , Hansbro PM 12 , Dua K 13

Affiliations 

  • 1 Centre of Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW, 2007, Australia
  • 2 School of Postgraduate Studies, International Medical University (IMU), 57000, Kuala Lumpur, Malaysia
  • 3 Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW, 2007, Australia
  • 4 Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto City, Kumamoto, 862-0973, Japan
  • 5 Center for Global Health Research, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, 602105, India
  • 6 Departamento de Química Orgánica, Facultad de Química y de Farmacia, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, 7820436, Santiago, Macul, Chile
  • 7 Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW, 2007, Australia
  • 8 School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia
  • 9 Aerogen, IDA Business Park, Dangan, Galway, H91 HE94, Ireland
  • 10 Woolcock Institute of Medical Research, University of Sydney, Sydney, New South Wales, Australia
  • 11 Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, 57000, Kuala Lumpur, Malaysia. Dinesh_Kumar@imu.edu.my
  • 12 Centre of Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW, 2007, Australia. philip.hansbro@uts.edu.au
  • 13 Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW, 2007, Australia. Kamal.Dua@uts.edu.au
Naunyn Schmiedebergs Arch Pharmacol, 2024 Apr;397(4):2465-2483.
PMID: 37851060 DOI: 10.1007/s00210-023-02760-7

Abstract

The purpose of this study was to evaluate the potential of zerumbone-loaded liquid crystalline nanoparticles (ZER-LCNs) in the protection of broncho-epithelial cells and alveolar macrophages against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro. The effect of the treatment of ZER-LCNs on in vitro cell models of cigarette smoke extract (CSE)-treated mouse RAW264.7 and human BCi-NS1.1 basal epithelial cell lines was evaluated for their anti-inflammatory, antioxidant and anti-senescence activities using colorimetric and fluorescence-based assays, fluorescence imaging, RT-qPCR and proteome profiler kit. The ZER-LCNs successfully reduced the expression of pro-inflammatory markers including Il-6, Il-1β and Tnf-α, as well as the production of nitric oxide in RAW 264.7 cells. Additionally, ZER-LCNs successfully inhibited oxidative stress through reduction of reactive oxygen species (ROS) levels and regulation of genes, namely GPX2 and GCLC in BCi-NS1.1 cells. Anti-senescence activity of ZER-LCNs was also observed in BCi-NS1.1 cells, with significant reductions in the expression of SIRT1, CDKN1A and CDKN2A. This study demonstrates strong in vitro anti-inflammatory, antioxidative and anti-senescence activities of ZER-LCNs paving the path for this formulation to be translated into a promising therapeutic agent for chronic respiratory inflammatory conditions including COPD and asthma.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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