Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549

Solanki N 1 , Mehta M 2 , Chellappan DK 3 , Gupta G 4 , Hansbro NG 5 , Tambuwala MM 6 Show all authors , Aa Aljabali A 7 , Paudel KR 5 , Liu G 5 , Satija S 2 , Hansbro PM 5 , Dua K 2 , Dureja H 1

Affiliations 

  • 1 Faculty of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, 124001, India
  • 2 Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney (UTS), Ultimo, NSW 2007, Australia
  • 3 Department of Life Sciences, School of Pharmacy, International Medical University (IMU), Bukit Jalil, Kuala Lumpur, 57000, Malaysia
  • 4 School of Pharmacy, Suresh Gyan Vihar University, Jagatpura, 302017, Mahal Road, Jaipur, India
  • 5 School of Life Sciences, Faculty of Science, University of Technology Sydney (UTS), Ultimo, NSW 2007, Australia
  • 6 School of Pharmacy & Pharmaceutical Sciences, Ulster University, Coleraine, County, Londonderry, BT52 1SA, Northern Ireland, UK
  • 7 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Yarmouk University, Irbid, 21163, Jordan
Future Med Chem, 2020 11;12(22):2019-2034.
PMID: 33124483 DOI: 10.4155/fmc-2020-0083

Abstract

Aim: In the present study boswellic acids-loaded chitosan nanoparticles were synthesized using ionic gelation technique. The influence of independent variables were studied and optimized on dependent variables using central composite design. Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48). The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively. Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells. The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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