Affiliations 

  • 1 Shri Baba Mastnath Institute of Pharmaceutical Sciences and Research, Baba Mastnath University, Rohtak 124001, India
  • 2 Department of Pharmaceutical Sciences, School of Pharmacy, International Medical University, Kuala Lumpur 57000, Malaysia
  • 3 School of Pharmacy and Pharmaceutical Sciences, Ulster University, Coleraine, County, Londonderry BT52 1SA, Northern Ireland, United Kingdom
  • 4 Discipline of Pharmacy, Graduate School of Health, University of Technology, Sydney 2007, Australia; Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI), School of Biomedical Sciences and Pharmacy, The University of Newcastle (UoN), Callaghan, NSW 2308, Australia; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia, 2308, Australia
  • 5 Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak 124001, India. Electronic address: harishdureja@gmail.com
Int J Biol Macromol, 2019 Dec 01;141:596-610.
PMID: 31494160 DOI: 10.1016/j.ijbiomac.2019.09.023

Abstract

The most common cause of deaths due to cancers nowadays is lung cancer. The objective of this study was to prepare erlotinib loaded chitosan nanoparticles for their anticancer potential. To study the effect of formulation variables on prepared nanoparticles using central composite design. Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using probe sonication technique. It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors. Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model. The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively. The IC50 value of NP-7 evaluated on A549 Lung cancer cells was found to be 6.36 μM. The XRD of NP-7 displayed the existence of erlotinib in the amorphous pattern. The optimized batch released erlotinib slowly in comparison to the marketed tablet formulation. Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release. The formulated nanoparticles can be utilized for the treatment of lung cancer.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.