Quercetin against MCF7 and CAL51 breast cancer cell lines: apoptosis, gene expression and cytotoxicity of nano-quercetin

Mohammed HA 1 , Sulaiman GM 2 , Anwar SS 2 , Tawfeeq AT 3 , Khan RA 1 , Mohammed SAA 4 Show all authors , Al-Omar MS 1 , Alsharidah M 5 , Rugaie OA 6 , Al-Amiery AA 7

Affiliations 

  • 1 Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Qassim, 51452, Saudi Arabia
  • 2 Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad,10066, Iraq
  • 3 Department of Molecular Biology, Iraqi Center for Cancer and Medical Genetics Research, Mustansiriyah University, PO Box 14022, Baghdad, Iraq
  • 4 Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Qassim, 51452, Saudi Arabia
  • 5 Department of Physiology, College of Medicine, Qassim University, Qassim, 51452, Kingdom of Saudi Arabia
  • 6 Department of Basic Medical Sciences, College of Medicine and Medical Sciences, Qassim University, Unaizah, PO Box 991, Qassim, 51911, Saudi Arabia
  • 7 Unit of Applied Sciences Research, Department of Applied Science, University of Technology, Baghdad,10066, Iraq
Nanomedicine (Lond), 2021 09;16(22):1937-1961.
PMID: 34431317 DOI: 10.2217/nnm-2021-0070

Abstract

Aims: To evaluate the anti breast-cancer activity, biocompatibility and toxicity of poly(d,l)-lactic-co-glycolic acid (PLGA)-encapsulated quercetin nanoparticles (Q-PLGA-NPs). Materials & methods: Quercetin was nano-encapsulated by an emulsion-diffusion process, and the nanoparticles were fully characterized through Fourier transform infrared spectroscopy, x-ray diffractions, FESEM and zeta-sizer analysis. Activity against CAL51 and MCF7 cell lines were assessed by DNA fragmentation assays, fluorescence microscopy, and acridine-orange, and propidium-iodide double-stainings. Biocompatibility towards red blood cells and toxicity towards mice were also explored. Results: The Q-PLGA-NPs exhibited apoptotic activity against the cell lines. The murine in vivo studies showed no significant alterations in the liver and kidney's functional biomarkers, and no apparent abnormalities, or tissue damages were observed in the histological images of the liver, spleen, lungs, heart and kidneys. Conclusion: The study established the preliminary in vitro efficacy and in vivo safety of Q-PLGA-NPs as a potential anti-breast cancer formulation.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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