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Fulltext Should hospital directors in public hospitals perform clinical work?

Roslan Johari, M.G., Teng, S.C., Rosidah, S.S., Haliza, A.M., Tahir, A., Nadhirah, R., et al.

Malaysian Journal of Public Health Medicine, 2005;5(1):44-50.
MyJurnal

A cross sectional study was conducted to determine the perception of Hospital Directors in performing clinical duties. This was done through a postal survey which was conducted covering all public hospitals in Malaysia. The majority of Hospital Directors had read the circular at the time of the study and agreed to do clinical work besides managerial duties. Before the issuance of the directive, the majority of those directors were already doing some clinical work. However they disagreed that by doing clinical work they could help overcome the problem of shortage of doctors. They agreed that the duration of 10 hours per week is suitable to do clinical duties. In term of confidence in giving curative treatment, the Hospital Directors especially those from district hospitals without specialists were confident in giving curative care to individuals. As for the clinicians, the majority felt that the implementation of the directive for Hospital Directors to do clinical work will not disrupt the clinicians' routine duties.
Study site: University Malaya, Universiti Kebangsaan Malaysia; Hospital Kuala Lumpur, Malaysia
Fulltext Medical conditions associated with tooth loss among Saudi adults

Hamasha, Abed Al-Hadi, Almogbel, Lolowh, Alshehri, Abeer, Alssafia, Fatimah, Alghamdi, Hanan, Alajmia, Alanoud, et al.

Archives of Orofacial Sciences, 2018;13(1):16-21.
MyJurnal

Upon reviewing the literature, the prevalence of many systemic conditions such as diabetes,
hypertension, asthma and rheumatoid arthritis were reported to be high in Saudi Arabia. The relationship of these
conditions with tooth loss among Saudi population was not investigated. Therefore, the aim of the present study
is to explore the relationship between tooth loss and most common medical conditions among Saudi dental
patient. The study participants were 250 patients who were randomly selected from the College of Dentistry
database of King Saud bin Abdulaziz University for Health Sciences (KSAU-HS) in Riyadh, Saudi Arabia.
Participants were requested to answer self-administered questionnaires related to their demographic as well as
general health questions concerned to the presence of systemic medical conditions. Missing teeth were
determined after examining the orthopantogram radiographs and reviewing the Romexis and SALUD databases.
Descriptive statistics, independent t-test and linear multiple regression model were performed using SPSS
software. The mean number of missing teeth among the study population was 5.8 teeth per person. The mean
number of missing teeth was higher among subjects with diabetes, hypertension, rheumatoid arthritis,
cardiovascular diseases, or osteoporosis compared to healthy individuals. A multiple linear regression analysis
model revealed that diabetes, hypertension and rheumatoid were significant predictors of missing teeth among
Saudi population. These results highlight the importance of the effect of medical conditions on oral health.
Fabrikasi dan pencirian pencerai 3x3 berasaskan gentian optik polimer (POF) dengan teknik pelakuran berkos rendah

Mohammad Syuhaimi Ab-Rahman, Latifah Sarah Supian, Hadi Guna, Nik Nur Syahirah Mohammad, Hwang IS, Afiq Hipni

Sains Malaysiana, 2014;43:1743-1750.

Gentian optik polimer (P0F) mempunyai banyak kelebihan dan banyak digunakan untuk komunikasi terutamanya dalam industri automotif. Komunikasi ini memerlukan maklumat yang banyak dihantar pada satu masa dengan kos yang rendah. Untuk itu pencerai 3x3 POF yang berkonsepkan pemultipleks bahagi jarak gelombang (wDM) yang berkos rendah dan berteknologi hijau difabrikasikan. Pencerai POF ini difabrikasikan dengan menggunakan alat yang murah dan mudah didapati, iaitu tiub besi dan lilM. Teknik fabrikasi yang digunakan adalah dengan memanaskan tiga lembar POF sehingga bahagian tengahnya mencair dan melakur sehingga mengecil dan memanjang berdiameter 1 mm. Pencerai 3x3 POF yang berjaya difabrikasi dicirikan dengan mengukur kehilangan POF sebelum dan selepas hujungnya diratakan dan digilap dengan dua jenis kertas pasir yang berlainan darjah kekasaran. Ini untuk melihat kesan gilapan pada kehilangan isyarat optik. Kehilangan pencerai PoF juga diukur pada suhu yang berbeza bagi melihat kesannya pada prestasi pencerai POF. Berdasarkan keputusan yang diperoleh, dapat dilihat bahawa kebersihan dan kerataan hujung POF memberi kesan kepada kehilangan dengan penurunan antara 0.3 hingga 5.0 dB. Suhu juga memberi kesan kepada kehilangan isyarat POF, walaupun tidak begitu ketara, dengan purata peningkatan kehilangan sebanyak 0.3 dB. Walaupun secara keseluruhannya nilai kehilangan dalam pencerai POF yang difabrikasi ini masih tinggi, prestasi ini dapat diperbaiki dengan penambahbaikan pada teknik pelakuran dan penggunaan alat yang lebih berkualiti. Teknik pemfabrikasi berkos rendah ini dipercayai boleh menghasilkan pencerai POF yang murah secara komersil, tetapi dengan kajian yang lebih mendalam pada teknik pelakuran, punca kehilangan dan cara meminimumkan kehilangan boleh diperoleh dan meningkatkan isyarat optik keluaran.
Fulltext Asyndromic trisomy X presented with premature ovarian failure: a case report

Ahzad Hadi Ahmad, Rabiatul Basria S.M.N. Mydin, Nur Ain Nisrina Roan, Abdul Rahman Azhari, Narazah Mohd Yusoff

Malaysian Journal of Medicine and Health Sciences, 2018;14(201):216-218.
MyJurnal

A 31-year-old lady with normal physical characteristics was found to have persistent high FSH and LH and was suspected possible premature ovarian failure after reported to have not normal menstrual cycle. Leucocytes were collected from patient’s fresh peripheral blood sample and Giemsa banding (G-banding) was done. All metaphases were captured and analysed using Cytovision software 4.5 and the final analysis show 47,XXX.
Fulltext Advanced paternal age effect on trisomy X syndrome

Ahzad Hadi Ahmad, Rabiatul Basria S.M.N. Mydin, Nur Ain Nisrina Roan, Abdul Rahman Azhari, Narazah Mohd Yusoff

Malaysian Journal of Medicine and Health Sciences, 2018;14(201):213-215.
MyJurnal

Advanced parental age is a risk factor for chromosomal abnormalities in their offspring. Trisomy X or Triple X syn- drome has previously been reported with advanced maternal age. Here we report two (2) cases of Trisomy X with paternal age as risk factor. Generally, Trisomy X individuals show variable physical and psychological manifesta- tions. However, both cases reported here have advanced paternal age as a risk factor; 55 years old (46 years old at conception) for Case 1 with patient having right eye squint, beaked nose, Posterior Misalignment Type Ventricular Septal Defect (PMVSD) and small Patent Ductus Arteriosus (PDA) with failure to thrive and 49 years old (45 years old at conception) for Case 2 with speech delay and protruding tongue. In view of that, advanced paternal age could possibly contribute the accumulation of de novo mutations in germ line mosaicism.
Fulltext Immunization knowledge and practice among Malaysian parents: a questionnaire development and pilot-testing

Awadh AI, Hassali MA, Al-lela OQ, Bux SH, Elkalmi RM, Hadi H

BMC Public Health, 2014;14:1107.
PMID: 25346471 DOI: 10.1186/1471-2458-14-1107

Parents are the main decision makers for their children vaccinations. This fact makes parents' immunization knowledge and practices as predictor factors for immunization uptake and timeliness. The aim of this pilot study was to develop a reliable and valid instrument in Malaysian language to measure immunization knowledge and practice (KP) of Malaysian parents.
Fulltext Does an educational intervention improve parents' knowledge about immunization? Experience from Malaysia

Awadh AI, Hassali MA, Al-Lela OQ, Bux SH, Elkalmi RM, Hadi H

BMC Pediatr, 2014;14:254.
PMID: 25284603 DOI: 10.1186/1471-2431-14-254

Parents' knowledge about immunization is an important predictor factor for their children's immunization status. The aims of this study were to assess parents' knowledge and to evaluate the effect of a short educational intervention on improving parents' knowledge of childhood immunization.
Fulltext Anthropometry and body composition status during Ramadan among higher institution learning centre staffs with different body weight status

Rohin MA, Rozano N, Abd Hadi N, Mat Nor MN, Abdullah S, Dandinasivara Venkateshaiah M

ScientificWorldJournal, 2013;2013:308041.
PMID: 24311975 DOI: 10.1155/2013/308041

This study was done to observe the anthropometry and body composition changes before, during, and after the holy month of Ramadan. This study was carried out on 46 staff from one of the local universities, which comprised of 14 males and 32 females ranging in age from 25 to 40 years old. There were four sessions done to complete this study, namely, a week before Ramadan (T1), 1st week of Ramadan (T2), 3rd week of Ramadan (T3), and a month after Ramadan (T4). All subjects were assessed according to weight, body circumference, and body composition status. It was found that subjects with different weight status showed a significant reduction in weight (P < 0.01) but no significant reduction in body fat percentage (P < 0.05). The findings suggest that weight reduction does not promise a reduction in body fat. Changes in neck circumference were only found in normal subjects. Hence, it can be said that overweight and obese subjects showed no changes in anthropometry status during Ramadan. No changes in body composition were reported in all three weight groups except for trunk body fat. In conclusion, normal subjects showed significant changes in various anthropometry parameters, but overweight and obese subjects showed no obvious difference.
Effectiveness of pharmacist-led medication review in chronic pain management: systematic review and meta-analysis

Hadi MA, Alldred DP, Briggs M, Munyombwe T, Closs SJ

Clin J Pain, 2014 Nov;30(11):1006-14.
PMID: 24480911 DOI: 10.1097/AJP.0000000000000063

OBJECTIVE: To evaluate the effectiveness of pharmacist-led medication review in chronic pain management.
MATERIALS AND METHODS: Six electronic databases (Medline, Embase, PsycInfo, CINHAL, CENTRAL, International Pharmaceutical Abstracts) reference lists of retrieved articles and relevant websites were searched for randomized controlled trials published in the English language involving adults with chronic pain. Studies were included if one of the intervention arms had received pharmacist-led medication review independently or as part of a multidisciplinary intervention. Risk of bias was assessed for all the included studies.
RESULTS: The search strategy yielded 583 unique articles including 5 randomized controlled trials. Compared with control, meta-analysis showed that participants in the intervention group had: a 0.8-point reduction in pain intensity on a 0 to 10 numerical rating scale at 3 months [95% confidence interval (CI), -1.28 to -0.36] and a 0.7-point reduction (95% CI, -1.19 to -0.20) at 6 months; a 4.84 point (95% CI, -7.38 to -2.29) and -3.82 point (95% CI, -6.49 to -1.14) improvement in physical functioning on a 0- to 68-point function subscale of Western Ontario and McMaster Universities Osteoarthritis Index at 3 and 6 months, respectively; and a significant improvement in patient satisfaction equivalent to a "small to moderate effect."
DISCUSSION: Pharmacist-led medication review reduces pain intensity and improves physical functioning and patient satisfaction. However, the clinical significance of these findings remain uncertain due to small effect size and nature of reported data within clinical trials that limits recommendation of wider clinical role of pharmacist in chronic pain management.
Fulltext Panduratin A, a possible inhibitor in metastasized A549 cells through inhibition of NF-kappa B translocation and chemoinvasion

Cheah SC, Lai SL, Lee ST, Hadi AH, Mustafa MR

Molecules, 2013 Jul 24;18(8):8764-78.
PMID: 23887718 DOI: 10.3390/molecules18088764

In the present study, we investigated the effects of panduratin A (PA), isolated from Boesenbergia rotunda, on apoptosis and chemoinvasion in A549 human non-small cell lung cancer cells. Activation of the executioner procaspase-3 by PA was found to be dose-dependent. Caspase-3 activity was significantly elevated at the 5 µg/mL level of PA treatment and progressed to a maximal level. However, no significant elevated level was detected on procaspase-8. These findings suggest that PA activated caspase-3 but not caspase-8. Numerous nuclei of PA treated A549 cells stained brightly by anti-cleaved PARP antibody through High Content Screening. This result further confirmed that PA induced apoptotic cell death was mediated through activation of caspase-3 and eventually led to PARP cleavage. Treatment of A549 cells with PA resulted in a strong inhibition of NF-κB activation, which was consistent with a decrease in nuclear levels of NF-κB/p65 and NF-κB/p50 and the elevation of p53 and p21. Besides that, we also showed that PA significantly inhibited the invasion of A549 cells in a dose-dependent manner through reducing the secretion of MMP-2 of A549 cells gelatin zymography assay. Our findings not only provide the effects of PA, but may also be important in the design of therapeutic protocols that involve targeting of either p53 or NF-κB.
Understanding the breast cancer experience: a qualitative study of Malaysian women

Yusuf A, Ab Hadi IS, Mahamood Z, Ahmad Z, Keng SL

Asian Pac J Cancer Prev, 2013;14(6):3689-98.
PMID: 23886167

Breast cancer is the most common and leading cause of cancer mortality among Malaysian women. Despite good survival rates, the diagnosis of cancer still invokes the feeling of stress, fear and uncertainty. Because very little is known about the experiences of Malaysian women with breast cancer, a qualitative study using semi- structured interviews to explore the lived experience of newly diagnosed breast cancer. Using a purposive sampling method, 20 Malaysian women newly diagnosed with breast cancer, including Malays (n=10) and Chinese (n=10) were recruited in two main public hospitals in Kelantan. Similarities and divergence in women's experience were identified through thematic analysis of interview transcripts. Three themes emerged from the data: uncertainty experience of the illness, transition process and fatalistic view of breast cancer. In many ways, these findings were parallel with previous studies, suggesting that the experience of breast cancer is to a certain extent similar among women newly diagnosed with breast cancer. This study adds to the sparse literature concerning the experience of illness following breast cancer diagnosis among the Malays and Chinese. More importantly, this study addressed areas that were previously lacking, specifically in depth information on breast cancer experience from a developing country with a multi-ethnic population. The results of this investigation provide preliminary information to healthcare professionals on the impact of illness and cultural influence on survivorship to plan for appropriate education and supportive programme in order to meet the needs of breast cancer women more effectively.
Fulltext Acute toxicity and gastroprotective role of M. pruriens in ethanol-induced gastric mucosal injuries in rats

Golbabapour S, Hajrezaie M, Hassandarvish P, Abdul Majid N, Hadi AH, Nordin N, et al.

Biomed Res Int, 2013;2013:974185.
PMID: 23781513 DOI: 10.1155/2013/974185

The investigation was to evaluate gastroprotective effects of ethanolic extract of M. pruriens leaves on ethanol-induced gastric mucosal injuries in rats. Forty-eight rats were divided into 8 groups: negative control, extract control, ulcer control, reference control, and four experimental groups. As a pretreatment, the negative control and the ulcer control groups were orally administered carboxymethylcellulose (CMC). The reference control was administered omeprazole orally (20 mg/kg). The ethanolic extract of M. pruriens leaves was given orally to the extract control group (500 mg/kg) and the experimental groups (62.5, 125, 250, and 500 mg/kg). After 1 h, CMC was given orally to the negative and the extract control groups. The other groups received absolute ethanol. The rats were sacrificed after 1 h. The ulcer control group exhibited significant mucosal injuries with decreased gastric wall mucus and severe damage to the gastric mucosa. The extract caused upregulation of Hsp70 protein, downregulation of Bax protein, and intense periodic acid schiff uptake of glandular portion of stomach. Gastric mucosal homogenate showed significant antioxidant properties with increase in synthesis of PGE2, while MDA was significantly decreased. The ethanolic extract of M. pruriens leaves was nontoxic (<5 g/kg) and could enhance defensive mechanisms against hemorrhagic mucosal lesions.
Fulltext Acute toxicity evaluation, antibacterial, antioxidant and immunomodulatory effects of Melastoma malabathricum

Alnajar ZA, Abdulla MA, Ali HM, Alshawsh MA, Hadi AH

Molecules, 2012;17(3):3547-59.
PMID: 22433579 DOI: 10.3390/molecules17033547

Melastoma malabathricum (MM) is a well-known plant in Malaysian traditional medicine, locally known as senduduk. Its ethanol and aqueous extracts have been used in the present investigation to study the immunomodulatory role on human peripheral blood mononuclear cell (PBMC), and the DPPH, ABTS and FRAP free radical scavenging activities were also measured. Total flavonoids and total phenolic contents were assayed and the antibacterial effect was tested against four species of bacteria; two Gram-positive (Staphylococcus aureus and Streptococcus agalactiae) and two Gram-negative (Escherichia coli and Klebsilla pneumonia). The tests were carried out using the disc diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. Moreover, the acute toxicity was evaluated in vivo on the ethanol extract of MM to establish its safety when administered orally. In our results, both extracts of MM showed abilities to scavenge DPPH and ABTS free radicals, IC(50) values: (11.599 ± 0.84, 10.573 ± 0.58 µmol/L) and (62.657 ± 0.78, 63.939 ± 0.48 µmol/L) for ethanol and aqueous extracts respectively. Indeed the ethanol extract evidenced high phenolic content (384.33 ± 0.005 mg/g), flavonoids contents (85.8 ± 0.009 mg/g) and ferric reducing antioxidant power (33,590 ± 0.038 mmol/g), with high activity against S. aureus and S. agalactiae (11 ± 0.3 and 12 ± 0.6 mm inhibition zones). Likewise, the percentage of peripheral blood mononuclear cells (PBMC) viability was increased in response to MM, IC(50) values (1.781 ± 1.2 and 6.545 ± 0.93 µg/mL) for ethanol and aqueous extracts, respectively. In addition, our results showed that the MM extract is safe even at a high dose of 5,000 mg/kg and has no oral toxicity. These findings suggest the excellent medicinal bioactivity of MM and explain the popularity of this plant in the folk medicine as a remedy for different illnesses.
Fulltext Ipomoea aquatica extract shows protective action against thioacetamide-induced hepatotoxicity

Alkiyumi SS, Abdullah MA, Alrashdi AS, Salama SM, Abdelwahab SI, Hadi AH

Molecules, 2012;17(5):6146-55.
PMID: 22617138 DOI: 10.3390/molecules17056146

In the Indian system of traditional medicine (Ayurveda) it is recommended to consume Ipomoea aquatica to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of I. aquatica against liver damage were evaluated in thioacetamide (TAA)-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD) rats were orally fed with I. aquatica (250 and 500 mg/kg) for two months along with administration of TAA (i.p injection 200 mg/kg three times a week for two months). The results showed that the treatment of I. aquatica significantly lowered the TAA-induced serum levels of hepatic enzyme markers (ALP, ALT, AST, protein, albumin, bilirubin and prothrombin time). The hepatic content of activities and expressions SOD and CAT that were reduced by TAA were brought back to control levels by the plant extract supplement. Meanwhile, the rise in MDA level in the TAA receiving groups also were significantly reduced by I. aquatica treatment. Histopathology of hepatic tissues by H&E and Masson trichrome stains displayed that I. aquatica has reduced the incidence of liver lesions, including hepatic cells cloudy swelling, infiltration, hepatic necrosis, and fibrous connective tissue proliferation induced by TAA in rats. Therefore, the results of this study show that the protective effect of I. aquatica in TAA-induced liver damage might be contributed to its modulation on detoxification enzymes and its antioxidant and free radical scavenger effects. Moreover, it confirms a scientific basis for the traditional use of I. aquatica for the treatment of liver disorders.
Fulltext Free radical scavenging, antimicrobial and immunomodulatory activities of Orthosiphon stamineus

Alshawsh MA, Abdulla MA, Ismail S, Amin ZA, Qader SW, Hadi HA, et al.

Molecules, 2012;17(5):5385-95.
PMID: 22569417 DOI: 10.3390/molecules17055385

Orthosiphon stamineus is considered an important traditional folk medicine. In this study ethanol and aqueous extracts of O. stamineus were evaluated in vitro for their antioxidant, antimicrobial as well as for their immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). The DPPH radical scavenging method was used for the determination of antioxidant activity, while the antibacterial efficacy was investigated by both disc diffusion method and Minimum Inhibitory Concentration (MIC) against four bacterial strains (Gram-positive and Gram-negative). Furthermore, the immunomodulatory potential of the extracts was investigated through the MTT assay. Aqueous extract of O. stamineus exhibited significant free radical scavenging activity with IC₅₀ 50 9.6 µg/mL, whereas the IC₅₀ for the ethanol extract was 21.4 µg/mL. The best antimicrobial activity was shown by the aqueous extract of O. stamineus against Staphylococcus aureus, with inhibition zone of 10.5 mm and MIC value 1.56 mg/mL. Moreover, the results observed from the MTT assay showed that both plant extracts stimulated the PBMCs proliferation in vitro in a concentration-dependent manner, but the aqueous extract has remarkable activity against PBMCs. These findings indicate that O. stamineus showed high antioxidant activity and may be considered as an immunomodulatory agent.
Gastroprotective activities of Turnera diffusa Willd. ex Schult. revisited: Role of arbutin

Taha MM, Salga MS, Ali HM, Abdulla MA, Abdelwahab SI, Hadi AH

J Ethnopharmacol, 2012 May 7;141(1):273-81.
PMID: 22374081 DOI: 10.1016/j.jep.2012.02.030

Turnera diffusa Willd. ex Schult. has been used for the treatment of several human disorders including peptic ulcer.
Awareness and knowledge of breast cancer and mammography among a group of Malaysian women in Shah Alam

Al-Dubai SA, Qureshi AM, Saif-Ali R, Ganasegeran K, Alwan MR, Hadi JI

Asian Pac J Cancer Prev, 2011;12(10):2531-8.
PMID: 22320951

OBJECTIVES: The aim of this study was to assess awareness and knowledge of breast cancer and mammography among Malaysian women in Shah Alam.
METHODS: This cross sectional study was conducted among 250 Malaysian women. Data were collected using a self administrated questionnaire which included questions on socio-demographic data, knowledge of breast cancer and awareness of mammography.
RESULTS: Mean age of respondents was 28 ± 9.2 with 69.2% aged 18 to 29 years. The majority had heard about breast cancer (81.2%) and indicated books, magazines and brochures as their source of information (55.2%). However, most did not know about signs and symptoms of breast cancer and many of its risk factors. On multivariate analysis, significant predictors of breast cancer knowledge were age, race, marital status, level of education, occupation, family size and family history of other cancers (p<0.05). Fifty percent of women were aware of mammography, significant predictors being age, occupation, marital status and knowledge of breast cancer (p<0.05).
CONCLUSION: Most women were aware of breast cancer. However, the knowledge about signs and symptoms of breast cancer and awareness of mammography were inadequate. It is recommended that the level of knowledge should be raised among Malaysian women, particularly in the less educated young.
Fulltext Naucline, a new indole alkaloid from the bark of Nauclea officinalis

Liew SY, Mukhtar MR, Hadi AH, Awang K, Mustafa MR, Zaima K, et al.

Molecules, 2012 Apr 02;17(4):4028-36.
PMID: 22469596 DOI: 10.3390/molecules17044028

A new indole alkaloid, naucline (1) together with four known alkaloids, angustine (2), angustidine (3), nauclefine (4) and naucletine (5), were isolated from the bark of Nauclea officinalis. The structures of all isolated compounds were elucidated with various spectroscopic methods such as 1D- and 2D- NMR, IR, UV and LCMS-IT-TOF. In addition to that of alkaloid 1, the complete 13C-NMR data of naucletine (5) were also reported. Naucline (1) showed a moderate vasorelaxant activity (90% relaxation at 1 × 10(-5) M) whereas, angustine (2), nauclefine (4), and naucletine (5) showed potent vasorelaxant activity (more than 90% relaxation at 1 × 10(-5) M) on an isolated rat aorta.
Fulltext N-cyanomethylnorboldine: a new aporphine isolated from Alseodaphne perakensis (Lauraceae)

Nafiah MA, Mukhtar MR, Omar H, Ahmad K, Morita H, Litaudon M, et al.

Molecules, 2011 Apr 20;16(4):3402-9.
PMID: 21512448 DOI: 10.3390/molecules16043402

A phytochemical study of the bark of Alseodaphne perakensis has yielded three aporphine alkaloids: the new compound N-cyanomethylnorboldine (1), and the two known alkaloids N-methyllaurotetanine (2) and norboldine (3). The isolation was achieved by chromatographic techniques and the structural elucidation was performed via spectral methods, notably 1D- and 2D-NMR, UV, IR, and HRFABMS. The vasorelaxation activity of compound 1 has been studied.
Fulltext Panduratin A inhibits the growth of A549 cells through induction of apoptosis and inhibition of NF-kappaB translocation

Cheah SC, Appleton DR, Lee ST, Lam ML, Hadi AH, Mustafa MR

Molecules, 2011 Mar 21;16(3):2583-98.
PMID: 21441862 DOI: 10.3390/molecules16032583

In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC₅₀ value of 4.4 µg/mL (10.8 µM). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as assessed by DNA condensation, nuclear morphology and intensity, cell permeability, mitochondrial mass/ potential, F-actin and cytochrome c. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in significant inhibition of Nuclear Factor-kappa Beta (NF-κB) translocation from cytoplasm to nuclei activated by tumor necrosis factor-alpha (TNF-α), as illustrated by the HCS assay. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in the A549 cell line, suggesting its therapeutic potential as an NF-κB inhibitor.

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