Displaying publications 221 - 240 of 488 in total

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  1. Barki N, Choudhry FR, Munawar K
    PMID: 33500886 DOI: 10.47176/mjiri.34.159
    Background: The construct of satisfaction with life has been studied across various cultures through the Satisfaction with Life Scale. The Satisfaction with Life Scale (SWLS) has been validated across several populations and languages. There are no published psychometric properties of its Urdu version. Hence, the aim of this study was to ascertain the psychometric properties of the Urdu version of the SWLS among the Urdu speaking population of Pakistan. Methods: The SWLS has already been translated into Urdu, and the Urdu version is available on the author's website however there is no information about its psychometric properties. To establish the psychometric properties especially the factor structure of the already translated Urdu SWLS, the SWLS-Urdu was administered to Urdu speaking population residing in Pakistan. The statistical analyses (i.e., normality through skewness and kurtosis, Kaiser-Meyer-Olkin (KMO) and Bartlett's test of sphericity, and test and re-test reliability) were conducted through SPSS version 25.0. Structure Equation Modelling via maximum likelihood method of estimation was used to perform confirmatory factor analysis on the data using AMOS 20.0. The significance level was set at p < 0.05. Results: The study was completed by recruiting 120 participants from different universities in Lahore, Pakistan. The sample was equally divided between male and female participants. The mean age of participants was 22.7(3.6) years. Test of the adequacy of the sample through Kaiser-Mayer-Olkin showed KMO=0.88 and Bartlett's test of sphericity (p<0.001). The Cronbach's alpha reliability of the scale was 0.90 and Confirmatory Factor Analysis confirmed a one-factor model as a good fit with strong statistical evidence. No factorial group variances were noticed in male and female participants. Conclusion: This study shows that Urdu SWLS has sound psychometric properties, is linguistically and culturally acceptable, and equally useful in assessing satisfaction with life in the Urdu speaking population.
    Matched MeSH terms: Pakistan
  2. Lal A, Sanaullah A, M Saleem MK, Ahmed N, Maqsood A, Ahmed N
    Eur J Dent, 2020 Dec;14(S 01):S27-S33.
    PMID: 33242914 DOI: 10.1055/s-0040-1718644
    OBJECTIVES:  The aim of this study was to evaluate psychological distress caused by the novel coronavirus disease 2019 (COVID-19) pandemic among the adult population residing in Pakistan.

    MATERIALS AND METHODS:  This cross-sectional survey-based study comprised 1,000 adults residing in Pakistan. A questionnaire was formulated and circulated among adult population of Pakistan, the depression and anxiety symptoms using Patient Health Questionnaire-9 (PHQ-9) and Generalized Anxiety Disorder-7 (GAD-7) scales were assessed.

    STATISTICAL ANALYSIS:  Independent t-test, cross tabulation, and regression analysis were used to identify variables having impact on PHQ-9 and GAD-7 scores. A p-value of ≤ 0.05 was considered statistically significant.

    RESULTS:  Among 1,000 participants, 573 were males and 427 were females who completed the survey. Majority were restricted to home for more than 40 days. Considerable number of participants reported depressive (540, 54%) and anxiety (480, 48%) symptoms. Gender, age, earnings, and occupation have significant relation with psychological distress, although similar was not found with education levels.

    CONCLUSION:  Psychological distress, a concerning yet addressable issue was found among adults arising amid COVID-19 outbreak. Currently, physical health effects of COVID-19 are being looked, while mental health effects being under-addressed. This issue should be addressed to avoid any psychological impact in future.

    Matched MeSH terms: Pakistan
  3. Lavelle CL, Flinn RM, Foster TD, Hamilton MC
    J Anat, 1970 Jul;107(Pt 1):182-3.
    PMID: 5473274
    Matched MeSH terms: Pakistan
  4. Khan AH, Israr M, Khan A, Aftab RA, Khan TM
    Am J Med Sci, 2015 Jun;349(6):505-9.
    PMID: 26030612 DOI: 10.1097/MAJ.0000000000000473
    BACKGROUND: Smoking is a risk factor not only for the development of cancer and coronary heart disease but also for tuberculosis (TB). The aim of this study was to determine the prevalence of smoking in patients with TB, identify demographic and clinical characteristics associated with smoking and to evaluate TB treatment outcomes in the smokers.
    METHOD: A retrospective cohort study of patients with TB was conducted at Khyber Teaching Hospital, Peshawar, Pakistan. All patients with pulmonary and extrapulmonary TB and those coinfected with HIV, hepatitis or diabetes mellitus were included in the study. The patients were categorized into smokers and nonsmokers. Treatment outcomes were evaluated by smear testing at the end of the treatment.
    RESULTS: Of 472 enrolled subjects, 68 (14.4%) were smokers. The prevalence of smoking among male and female patients with TB was 11.8% and 2.5%, respectively. Univariate analysis indicated that the gender, age group and marital status of patients with TB were associated with smoking. The results indicated that patient gender (P = 0.05), age: 15 to 24 years (P = 0.05) and age >55 years (P = 0.004) were risk factors associated with smoking among TB patients. Of the 68 smokers with TB, the treatment outcomes among 54 patients (79.4%) were unsuccessful. The treatment outcomes was statistically significantly associated with smoking (odds ratio: 2.58, P = 0.004).
    CONCLUSIONS: Findings from the current study proved smoking to be one of the main factors associated with the occurrence of TB and significantly reducing the outcomes of TB therapy.
    Matched MeSH terms: Pakistan
  5. Arthur D, Foong A, McMaster R, Cleary M
    Issues Ment Health Nurs, 2020 Mar;41(3):261-264.
    PMID: 31917620 DOI: 10.1080/01612840.2019.1701931
    Matched MeSH terms: Pakistan
  6. Waqas M, Iqbal SZ, Abdull Razis AF, Pervaiz W, Ahmad T, Usman S, et al.
    PMID: 34360308 DOI: 10.3390/ijerph18158015
    Aflatoxins (AFs) are secondary metabolites toxic to humans as well as animals. The environmental conditions, conventional agricultural practices, and illiteracy are the main factors which favor the production of AFs in food and feed. In the current study 744 samples of vegetable seeds and oils (soybean, sunflower, canola, olive, corn, and mustard) were collected and tested for the presence of aflatoxin B1 (AFB1) and total AFs. Liquid-liquid extraction was employed for the extraction of AFs from seeds and oil samples. Reverse phase high performance liquid chromatography equipped with fluorescence detection was used for the analysis. The results have shown that 92 (56.7%) samples of imported and 108 (57.0%) samples of local edible seeds were observed to be contaminated with AFs. All samples of edible seeds have AFB1 levels greater than the proposed limit set by the European Union (EU, 2 µg/kg) and 12 (7.40%) samples of imported seeds and 14 (7.40%) samples of local seeds were found in the range ≥ 50 µg/kg. About 78 (43.3%) samples of imported edible oil and 103 (48.3%) sample of local edible oil were observed to be positive for AFs. Furthermore, 16 (8.88%) and six (3.33%) samples of imported vegetable oil have levels of total AFs in a range (21-50 µg/kg) and greater than 50 µg/kg, respectively. The findings indicate significant differences in AFs levels between imported and local vegetable oil samples (t = 22.27 and p = 0.009) at α = 0.05 and a significant difference in AFs levels were found between vegetable seeds and oil samples (t = -17.75, p = 0.009) at α = 0.05. The highest dietary intake was found for a local sunflower oil sample (0.90 µg/kg/day) in female individuals (16-22 age group). The results have shown considerably high levels of AFB1 and total AFs in seeds and oil samples and emphasise the need to monitor carefully the levels of these toxic substances in food and feed on regular basis.
    Matched MeSH terms: Pakistan
  7. Thai VN, Dehbandi R, Fakhri Y, Sarafraz M, Nematolahi A, Dehghani SS, et al.
    Biol Trace Elem Res, 2021 Sep;199(9):3497-3509.
    PMID: 33180263 DOI: 10.1007/s12011-020-02476-2
    The contamination of seafood like narrow-barred Spanish mackerel (Scomberomorus commerson) fillets by potentially toxic elements (PTEs) has converted to worldwide health concerns. In this regard, the related citations regarding the concentration of PTEs in fillets of narrow-barred Spanish mackerel were collected through some of the international databases such as Scopus, Cochrane, PubMed, and Scientific Information Database (SID) up to 10 March 2020. The concentration of PTEs in fillets of narrow-barred Spanish mackerel fish was meta-analyzed and the health risk (non-carcinogenic risk) was estimated by the total target hazard quotient (TTHQ). The meta-analysis of data indicated that the rank order of PTEs in fillet of narrow-barred Spanish mackerel was Fe (10,853.29 μg/kg-ww) > Zn (4007.00 μg/kg-ww) > Cu (1005.66 μg/kg-ww) > total Cr (544.14 μg/kg-ww) > Mn (515.93 μg/kg-ww) > Ni (409.90 μg/kg-ww) > Pb (180.99 μg/kg-ww) > As (93.11 μg/kg-ww) > methyl Hg (66.60 μg/kg-ww) > Cd (66.03 μg/kg-ww). The rank order of health risk assessment based on the country by the aid of TTHQ for adult consumers was Malaysia (0.22251) > Philippines (0.21912) > Egypt (0.08684) > Taiwan (0.07430) > Bahrain (0.04893) > Iran (0.03528) > China (0.00620) > Pakistan (0.00316) > Yemen (0.00157) > India (0.00073). In addition, the rank order of health risk assessment based on the country by the aid of TTHQ for child consumers was Malaysia (1.03838) > Philippines (1.02257) > Egypt (0.40523) > Taiwan (0.34674) > Bahrain (0.22832) > Iran (0.16466) > China (0.02892) > Pakistan (0.01474) > Yemen (0.00731) > India (0.00340). Therefore, the children in Malaysia and the Philippines were at considerable non-carcinogenic risk. Hence, approaching the recommended control plans in order to decrease the non-carcinogenic risk associated with the ingestion of PTEs via the consumption of narrow-barred Spanish mackerel fish fillets is crucial.
    Matched MeSH terms: Pakistan
  8. Hayat K, Mustafa ZU, Godman B, Arshed M, Zhang J, Khan FU, et al.
    Front Pharmacol, 2021;12:650137.
    PMID: 34054528 DOI: 10.3389/fphar.2021.650137
    Background: Optimal collaboration between pharmacists and other healthcare professionals such as physicians is integral in implementing pharmaceutical care. However, there are concerns regarding the role of pharmacists, especially among low- and middle-income countries. This study explored the perceptions, expectations, and experience of physicians working in various hospital settings of Punjab, Pakistan, about pharmacists and their roles. Methods: A self-administered questionnaire consisting of four sections was administered from October to December 2020. Descriptive and inferential statistics such as Kruskal-Wallis and Mann-Whitney tests were used for data analysis using SPSS. Results: Six hundred and seventy-eight physicians participated in this study with a response rate of 77.9%. Most of the physicians reported minimal to no interaction with pharmacists (n = 521, 76.8%). However, more than three-quarters of physicians (n = 660, 97.3%) accepted pharmacists as evidence-based sources of drug information. In addition, many physicians (n = 574, 84.7%) strongly agreed that pharmacists should attend patient care rounds to respond promptly to questions related to patient medication. A limited number of physicians (n = 124, 18.3%) assumed that pharmacists were advising their patients regarding the judicial use of their drugs. Median expectation and experience score had a significant association with age, experience, and education of physicians (P < 0.05). Conclusions: The perception of physicians was positive toward certain roles of pharmacists, coupled with high expectations. However, their experience was low, with most of the activities of pharmacists due to inadequate interprofessional coordination.
    Matched MeSH terms: Pakistan
  9. Dash NR, Guraya SY, Al Bataineh MT, Abdalla ME, Yusoff MSB, Al-Qahtani MF, et al.
    BMC Med Educ, 2020 Nov 30;20(1):480.
    PMID: 33256705 DOI: 10.1186/s12909-020-02358-0
    BACKGROUND: In the current wave of educational reforms, understanding teaching styles of medical faculty can help modify instructional strategies for effective teaching. Few studies have probed distinctive teaching styles of medical faculty. We compared preferred teaching styles of faculty from seven medical schools in United Arab Emirates, the Netherlands, Saudi Arabia, Malaysia, Pakistan, and Sudan.

    METHODS: The validated Grasha-Riechmann teaching style inventory was administered online for data collection and used SPSS version 20.0 for statistical analysis.

    RESULTS: Of the 460 invitees, 248 responded (response rate; 54%). Delegator teaching style was most common with a highest median and mean of 2.38 and 2.45, respectively. There was a significant correlation between expert and authority teaching styles, correlation coefficient 0.62. Similarly, we found a significant correlation between authority teaching style and nature of curriculum, correlation coefficient 0.30. Multiple regression analysis showed that only authority teaching style and male gender had significant correlation. Interestingly, 117 (47%) teachers disagreed with the teaching philosophy of delivering course contents by strictly following learning outcomes. Female teachers (114/248) were more willing to negotiate with their students regarding how and what to teach in their course, while male teachers tended to allow more autonomy by allowing students to set their learning agenda.

    CONCLUSIONS: This study showed that the medical teachers preferred delegator teacher style that promotes students' collaboration and peer-to-peer learning. Most teachers are conscious of their teaching styles to motivate students for scientific curiosity. These findings can help medical educators to modify their teaching styles for effective learning.

    Matched MeSH terms: Pakistan
  10. Gunjal S, Pateel DGS, Yang YH, Doss JG, Bilal S, Maling TH, et al.
    Subst Use Misuse, 2020;55(9):1533-1544.
    PMID: 32569533 DOI: 10.1080/10826084.2019.1657149
    Background: Areca nut (AN) and betel quid (BQ) chewing are ancient practices followed by an extensive proportion of the world's population. These practices are endemic in larger parts of South and Southeast Asia and selected Western Pacific countries. The prevalence of these habits varies across regions, age, gender, cultural practice, and socioeconomic status groups. Considerable variations exist between countries with respect to prevention/intervention programs, and policy guidelines of BQ usage. Objectives: (1) To provide an overview of the BQ chewing prevalence, practices, preventive interventions and policies in selected Asian and Western Pacific countries. (2) To explore the different terminologies associated with BQ use. Method: A narrative review of the current literature related to BQ, AN, and oral cancer was conducted by searching PUBMED, CINAHL, and GOOGLE databases. Results: The literature review revealed that the prevalence of BQ was found to be highest in Papua New Guinea, followed by Bangladesh, India, Pakistan, Myanmar and Sri Lanka. While, Cambodia, Malaysia, Indonesia and Taiwan had comparatively lower prevalence. Smokeless tobacco, BQ with tobacco, BQ without tobacco, AN were some of the terminologies used for BQ in various studies. Conclusions: The prevalence, and the interventional policies related to BQ and AN chewing habits varies widely among the selected countries. With the increasing awareness and association of BQ with oral cancer, there is a need to have better awareness, prevention and interventional strategies in place. We also found considerable variation in the use of terminologies associated with BQ.
    Matched MeSH terms: Pakistan
  11. Liaquat S, Qayyum M, Ahmed H, Arfeen RZU, Celik F, Simsek S
    Trop Biomed, 2021 Jun 01;38(2):1-8.
    PMID: 33973567 DOI: 10.47665/tb.38.2.031
    Goat Warble Fly Infestation (GWFI) is also known as subcutaneous myiasis caused by Przhevalskiana silenus (Diptera: Oestridae). It is widely distributed in tropical and sub-tropical areas of the world. In goats, WFI is usually detected through conventional procedure which underestimated the infestation. The current study was designed to determine the serodiagonsis of GWFI (through IDEXX Hypodermosis serum antibody test) and also aimed to investigate its seroepizootiological profile in Pothwar region, Pakistan from 2013-14. The results showed that average seropositivity (ELISA kit) of GWFI was 18.5% whereas, it was 11% by using conventional procedure (Palpation method) depicting a significant difference (p<0.05). Higher seropositivity (30.8%) was observed in Jhelum district as compared to e Attock district (6%). The L1 larvae were found in September, while nodules start appearing in October to December and last until the end of February. The month wise peaks of optical density (OD) was higher in December which gradually decrease along with the end of winter season. The prevalence of GWFI revealed no significant difference among three host breeds (Jattal, Beetal and Tedy). According to the results, high infestation rate (28%) was observed in young animals of age group < 1 year as compared to old animals (> 2 years). Topographically, hilly areas (33%) provide favourable climatic conditions for the propagating of larval stages. Sex difference showed no significant difference. The seroprevalence varied significantly with respect to age, month, districts and topographical location. The current study proved that serologic diagnosis (commercial ELISA kit) as more sensitive and accurate for timely diagnosis of GWFI than traditional method. The information on the epizootiology of P. silenus in goats of Pothwar region would help in devising effective control strategies.
    Matched MeSH terms: Pakistan
  12. Rogers EM, Solomon DS
    Stud Fam Plann, 1975 May;6(5):126-33.
    PMID: 1145693 DOI: 10.2307/1964747
    The objectives of this article are (1) review the contribution of traditional midwives to family planning communication in several Asian countries; (2) organize knowledge gathered from various studies into general guidelines for the most effective use of traditional midwives in family planning programs; and (3) present hypotheses for future research. In certain countries where pilot projects have tested the potential performance of traditional midwives in family planning programs, results have been encouraging. In other nations, more research is needed to determine the contribution traditional midwives can make to the family planning program.
    PIP: Traditional midwives are found in almost every village and in many urban neighborhoods in the developing world, delivering the majority of births in these areas. Several Asian nations have begun to recognize the potential contribution of traditional midwives to modern family planning and health programs. A total of about 17,000 traditional midwives have been trained as family planning recruiters in Indonesia, Malaysia, the Philippines, and Thailand. Because traditional midwives deliver a large number of births in Asian nations, the potential is great for them to reach large numbers of women regarding family planning -- particularly poor, illiterate women. There is much to be learned from the traditional health system and governmental health and family planning programs should join hands with these older systems.
    Matched MeSH terms: Pakistan
  13. Westoff CF
    Fam Plann Perspect, 1978 May-Jun;10(3):173-81.
    PMID: 658326
    The unmet need for family planning services is remarkably constant across all five countries because of the interaction of fertility intentions and fertility control: as more women use contraception, more of them want fewer children.
    Matched MeSH terms: Pakistan
  14. Oh AMF, Tan CH, Ariaranee GC, Quraishi N, Tan NH
    J Proteomics, 2017 07 05;164:1-18.
    PMID: 28476572 DOI: 10.1016/j.jprot.2017.04.018
    The Indian krait (Bungarus caeruleus) is one of the "Big Four" venomous snakes widely distributed in South Asia. The present venomic study reveals that its venom (Sri Lankan origin) is predominated by phospholipases A2 (64.5% of total proteins), in which at least 4.6% are presynaptically-acting β-bungarotoxin A-chains. Three-finger toxins (19.0%) are the second most abundant, comprising 15.6% κ-neurotoxins, the potent postsynaptically-acting long neurotoxins. Comparative chromatography showed that venom samples from Sri Lanka, India and Pakistan did not exhibit significant variation. These venoms exhibited high immunoreactivity toward VINS Indian Polyvalent Antivenom (VPAV). The Pakistani krait venom, however, had a relatively lower degree of binding, consistent with its moderate neutralization by VPAV (potency=0.3mg venom neutralized per ml antivenom) while the Sri Lankan and Indian venoms were more effectively neutralized (potency of 0.44 mg/ml and 0.48 mg/ml, respectively). Importantly, VPAV was able to neutralize the Sri Lankan and Indian venoms to a comparable extent, supporting its use in Sri Lanka especially in the current situation where Sri Lanka-specific antivenom is unavailable against this species. The findings also indicate that the Pakistani B. caeruleus venom is immunologically less comparable and should be incorporated in the production of a pan-regional, polyspecific antivenom.

    BIOLOGICAL SIGNIFICANCE: The Indian krait or blue krait, Bungarus caeruleus, is a highly venomous snake that contributes to the snakebite envenoming problem in South Asia. This is a less aggressive snake species but its accidental bite can cause rapid and severe neurotoxicity, in which the patient may succumb to paralysis, respiratory failure and death within a short frame of time. The proteomic analysis of its venom (sourced from Sri Lanka) unveils its content that well correlates to its envenoming pathophysiology, driven primarily by the abundant presynaptic and postsynaptic neurotoxins (β-bungarotoxins and κ-neurotoxins, respectively). The absence of cytotoxins in the venom proteome also correlates with the lack of local envenoming sign (pain, swelling), and explains why the bite may be insidious until later stage when paralysis sets in. The muscarinic toxin-like proteins in the venom may be the cause of severe abdominal pain that precedes paralysis in many cases, and justifies the need of closely monitoring this symptom in suspected cases. Venom samples from Sri Lanka, India and Pakistan exhibited no remarkable variation in protein profiling and reacted immunologically toward the VINS Indian Polyvalent Antivenom, though to a varying extent. The antivenom is effective in neutralizing the Sri Lankan and Indian venoms, confirming its clinical use in the countries. The antivenom efficacy against the Pakistani venom, however, may be further optimized by incorporating the Pakistani venom in the antivenom production.

    Matched MeSH terms: Pakistan
  15. Laghari M, Sulaiman SAS, Khan AH, Memon N
    PeerJ, 2018;6:e5253.
    PMID: 30065869 DOI: 10.7717/peerj.5253
    Background: Regardless of the advancement in medical technologies, the diagnosis of tuberculosis (TB) in children has remained a challenge. Childhood TB is rampant and an important cause of morbidity and mortality. The objective of this study was to determine the trend of TB and treatment outcomes in children aged ≤14 years registered for TB treatment under DOTS course in three districts of Sindh, Pakistan.

    Methods: For this retrospective study, records of TB children (≤14 years) registered for the treatment of TB from January 2011 to December 2015 in three districts of Pakistan, were collected. Demographic data, baseline weight, clinical manifestations, radiography, histopathology results and treatment outcomes were collected from TB unit registers.

    Results: A total of 2,167 children were treated for TB during the study period. Of these, 1,199 (55.3%) were females and 1,242 (57.3%) were from urban areas. Over three-quarter of patients (76.9%) had pulmonary TB with 13.3% of sputum smear positive cases. The overall treatment success rate was 92.4%. In multivariate analysis, rural residents (OR: 2.146, p 

    Matched MeSH terms: Pakistan
  16. Hussain R, Hassali MA, Hashmi F, Farooqui M
    PMID: 30034811 DOI: 10.1186/s40545-018-0143-0
    Background: Medication safety is a major public health concern and there are well established pharmacovigilance programmes in developed countries. However, there is scarcity of literature on the issue in low and middle income countries. In this context, the current study was aimed to evaluate the knowledge, attitudes and practices of hospital pharmacists towards medication safety and ADR reporting in Lahore, Pakistan.

    Methods: A qualitative approach was used to conduct this study. A semi-structured interview guide was developed, 10 hospital pharmacists were recruited and interviewed through convenience sampling technique. All interviews were audio-taped, transcribed verbatim, and were then analyzed for thematic contents analysis.

    Results: Thematic content analysis of the interviews resulted in 6 major themes, including (1) Familiarity with medication safety & adverse drug reaction concept (2) Current system of practice and reporting of adverse drug reaction in hospital setting, (3) Willingness to accept the practice change (4) Barriers to adverse drug reaction reporting, (5) Policy change needs and (6) The recognition of the role. Majority of the hospital pharmacists were familiar with the concept of medication safety and ADR reactions reporting however they were unaware of the existence of national ADR reporting system in Pakistan. Several barriers hindering ADR reporting were identified including lack of awareness and training, communication gap between the hospitals and regulatory authorities.

    Conclusion: The study revealed that that hospital pharmacists were good in understanding of medication safety and ADR reporting; however they don't practice this in real sense. The readiness of the hospital pharmacist towards the practice change has indicated that they are all set to be actively involved in the provision of medication safety in hospital setting. Involvement of key stake holders from ministry of health, academia, pharmaceutical industry and healthcare professionals is warranted to promote safe and effective use of medicines.

    Matched MeSH terms: Pakistan
  17. Ahmad M, Zafar M, Sultana S, Ahmad M, Abbas Q, Ayoub M, et al.
    Microsc Res Tech, 2018 Sep;81(9):1004-1016.
    PMID: 30303585 DOI: 10.1002/jemt.23066
    Pollen micro-morphological features have proven to be helpful for the plant taxonomists in the identification and classification of plants. The utilization of this plantmayhelpfulin the areas of lignocellulosic conversion to biofuels and diversify application toward biomass. The current study was planned with the aim to evaluate the pollen features of complex Ranunculaceous flora of District Chitral, Northern Pakistan using both scanning electron microscopy (SEM) and Light Microscope (LM) for their taxonomic importance. Pollens of 18 Ranunculaceous species belonging to 6 genera were collected from different localities of the research area. SEM and LM were used to examine both qualitative and quantitative micro-morphological features. Sculptring of the sexine include; Scabrate, psilate, echinate, verrucate, perforate gemmate, and reticulate and so forth. Shape of the pollens was sub-spheroidal, spheroidal, prolate, subprolate and oblate and so forth. Type of pollen was ranged from mono to tricolpate and tricolporate. Quantitative characters include length/width of the pollen, colpus, exine thickness, and P/E ratio. Based on these micro-morphological features a taxonomic key was prepared for the fast and correct identification. RESEARCH HIGHLIGHT: Study of the pollen micro-morphological features of Ranunculaceous species by SEM and LM. Analysing both qualitative and quantitative characters of the pollens. Preparation of taxonomic key based on micro-morphological features for the correct and fast identification.
    Matched MeSH terms: Pakistan
  18. Arshad S, Ahmad M, Saboor A, Ibrahim FH, Mustafa MRU, Zafar M, et al.
    Microsc Res Tech, 2019 Feb;82(2):92-100.
    PMID: 30511479 DOI: 10.1002/jemt.23106
    Climate change is the most realistic theory of this era. Sudden and drastic changes are happening on the earth and the survival of mankind is becoming questionable in the future. The plants play the key role in controlling the climate change. The study emphasizes on role of trees in the cop up or damaging the climate of this earth, whether they are medicinal trees or economically important trees. Due to the overgrazing and intense deforestation the climate is being affected hazardously. The global warming phenomenon is occurring due to the less availability of trees and more carbon dioxide in the atmosphere. In total 20 plants were collected from across the Pakistan on the basis of their abundance and their key roles. Out of which seeds of eight plants were scanned through scanning electron microscope for correct authentication and importance of these medicinally important trees in mitigating the climate change. RESEARCH HIGHLIGHTS: The role of forest sector in the climate's change mitigation. Medicinally and economically important tree species across Pakistan. By using SEM, Ultra seed sculpturing features as an authentication tool. To formulate some policies to stop or control deforestation.
    Matched MeSH terms: Pakistan
  19. Moura de Aguiar D, Pessoa Araújo Junior J, Nakazato L, Bard E, Aguilar-Bultet L, Vorimore F, et al.
    Microorganisms, 2019 Nov 05;7(11).
    PMID: 31694172 DOI: 10.3390/microorganisms7110528
    The genus Ehrlichia is composed of tick-borne obligate intracellular gram-negative alphaproteobacteria of the family Anaplasmataceae. Ehrlichia includes important pathogens affecting canids (E. canis, E. chaffeensis, and E. ewingii), rodents (E. muris), and ruminants (E. ruminantium). Ehrlichiaminasensis, an Ehrlichia closely related to E. canis, was initially reported in Canada and Brazil. This bacterium has now been reported in Pakistan, Malaysia, China, Ethiopia, South Africa, and the Mediterranean island of Corsica, suggesting that E. minasensis has a wide geographical distribution. Previously, E. minasensis was found to cause clinical ehrlichiosis in an experimentally infected calf. The type strain E. minasensis UFMG-EV was successfully isolated from Rhipicephalus microplus ticks and propagated in the tick embryonic cell line of Ixodes scapularis (IDE8). However, the isolation and propagation of E. minasensis strains from cattle has remained elusive. In this study, the E. minasensis strain Cuiabá was isolated from an eight-month-old male calf of Holstein breed that was naturally infected with the bacterium. The calf presented clinical signs and hematological parameters of bovine ehrlichiosis. The in vitro culture of the agent was established in the canine cell line DH82. Ehrlichial morulae were observed using light and electron microscopy within DH82 cells. Total DNA was extracted, and the full genome of the E. minasensis strain Cuiabá was sequenced. A core-genome-based phylogenetic tree of Ehrlichia spp. and Anaplasma spp. confirmed that E. minasensis is a sister taxa of E. canis. A comparison of functional categories among Ehrlichia showed that E. minasensis has significantly less genes in the 'clustering-based subsystems' category, which includes functionally coupled genes for which the functional attributes are not well understood. Results strongly suggest that E. minasensis is a novel pathogen infecting cattle. The epidemiology of this Ehrlichia deserves further attention because these bacteria could be an overlooked cause of tick-borne bovine ehrlichiosis, with a wide distribution.
    Matched MeSH terms: Pakistan
  20. Hanif M, Khattak M, Amin M, Ramzan M, Zakir S, Ullah S, et al.
    Sains Malaysiana, 2016;45:489-497.
    A 1.7 m2 flat plate solar air heater was designed and developed in the Department of Agricultural Mechanization, The University of Agriculture Peshawar, Pakistan in collaboration with the Department of Environmental Sciences, University of Peshawar, Pakistan. It was operated under an average solar irradiance of 0.9 kJ.m-2.h-1 in the month of September, 2013. It worked under an efficiency of 7.5 to 21%. The heat collected by the air heater was given to air flowing as a medium inside it. This hot air was given to a drying section and water heating tank for drying and water heating purposes. The drying section provided a temperature in the range of 40-50oC and humidity of 10-30% from 10:00 am to 3:00 pm. The water heating tank provides hot water with a temperature of 35- 45oC from 10:00 am to 5:00 pm. Furthermore, the drying section was used to dry apples, onions and persimmons. All the products showed a consistent moisture loss from them with an optimum drying rate. The two term exponential model showed that all the three products dried have a good correlation with drying time with R2 values higher than 0.90. It was concluded that flat plate solar air heaters is the best technology for water heating and drying purposes and is a good alternative of conventional energy sources.
    Matched MeSH terms: Pakistan
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