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  1. Gima E, Teh CSL, Nik Hassan NFH, Yaacob NM, Md Shukri N
    Malays J Med Sci, 2023 Dec;30(6):156-166.
    PMID: 38239243 DOI: 10.21315/mjms2023.30.6.15
    BACKGROUND: Olfactory disorders (OD) are an umbrella term for a diverse group of smell problems. Numerous tests and questionnaires have been formulated to identify and test the severity of smell impairment, which is not readily available or translated for the Malaysian population. This study aimed to translate the Questionnaire for Olfactory Disorders (QOD) and validate and test the reliability of the Malay Questionnaire for Olfactory Disorders (mQOD).

    METHODS: This cross-sectional study was conducted in two tertiary centres. A forward and backward translation was conducted for the QOD. The translated questionnaire was distributed to subjects with self-reported smell disorders on days 1 and 7. Internal consistency was analysed using Cronbach's alpha and test-retest reliability was tested with an intraclass correlation coefficient. Confirmatory factor analysis was performed to test construct validity.

    RESULTS: A total of 375 participants were recruited, 52 dropped out and 323 completed the questionnaire a second time. The Cronbach's alpha coefficient was 0.537 for parosmia (P), 0.892 for life quality (LQ), 0.637 for sincerity (S) and 0.865 for visual analogue score (VAS). The intraclass correlation coefficient (ICC) for domain scores was > 0.9, while the ICC for all items was good to excellent. A three-factor model for mQOD showed an acceptable fit with indices chi-square value (CMIN)/degree of freedom (DF) = 3.332, Tucker-Lewis fit index (TLI) = 0.923, comparative fit index (CFI) = 0.939, root mean square error of approximation (RMSEA) = 0.079 and standardised root mean square residual (SRMR) = 0.0574.

    CONCLUSION: The mQOD is a valid and reliable tool for assessing OD in patients.

  2. Md Nizar ND, Hassan SK, Mohamad Zaini RH, Hassan MH, Wan Hassan WMN, Mazlan MZ
    Malays J Med Sci, 2020 Dec;27(6):68-78.
    PMID: 33447135 DOI: 10.21315/mjms2020.27.6.7
    BACKGROUND: Hypotension is a common complication following spinal anaesthesia. The administration of intravenous fluids prior to spinal anaesthesia, known as pre-loading, has been used to offset the hypotension effect; however, the ideal fluid for pre-loading is still a matter of debate. The objective of this study was to compare the effects of Gelaspan 4% and Volulyte 6% as pre-loading fluids.

    METHODS: A total of 93 patients with American Society of Anaesthesiologists (ASA) physical status I or II having lower limb orthopaedic surgery under spinal anaesthesia were randomised into two groups that received either Volulyte (n = 47) or Gelaspan (n = 46). Before the spinal anaesthesia, these patients were pre-loaded with 500 mL of the fluid of their respective group. Blood samples were taken before pre-loading and again after spinal anaesthesia and sent for venous blood gas and electrolyte level measurement. Baseline and intraoperative records of systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), heart rate (HR) and the requirement of ephedrine to treat hypotension were also recorded.

    RESULTS: Both fluids could not prevent significant reductions in SBP (P = 0.011), DBP (P = 0.002) and MAP (P = 0.001). There was also significant reduction in HR over time (P < 0.001). There was no significant difference in terms of ephedrine usage between both groups. Neither Volulyte 6% nor Gelaspan 4% caused significant changes in acid-base status.

    CONCLUSION: The use of 500 mL of either Gelaspan 4% or Volulyte 6% as pre-loading fluids did not significantly prevent the incidence of post-spinal anaesthesia hypotension following orthopaedic lower limb surgery; however, both were useful in the maintenance normal acid-base balance.

  3. Hashmi SI, Ah Gang GC, Sombuling A, Md Nawi NH, Megat Ahmad PH
    Malays J Med Sci, 2021 Dec;28(6):108-120.
    PMID: 35002495 DOI: 10.21315/mjms2021.28.6.11
    Introduction: The Malay autism spectrum quotient, children's version (AQ-Child) is a translated and reduced version of the autism spectrum disorder (ASD) screening instrument. The aim of this study was to test the reliability and validity of the translated Malay version of the questionnaire.

    Methods: The instrument is a 41-item Likert scale form parental questionnaire designed to screen ASD among children aged 6 years old-12 years old. It was filled out by the parents of 700 children (children with ASD = 47; typically developing children = 653) who were contacted through five special education schools and seven mainstream primary schools.

    Results: Findings showed that Malay AQ-Child has an internal consistency of 0.82 as a whole scale for 41-items. Receiver operator characteristic analysis showed that the cut score of 63 for the translated, reduced version of the AQ-Child with 41 items had 99% area (95% confidence interval [CI]) under receiver operating characteristic (ROC) curve, and sensitivity and specificity of 93% and 99%, respectively. As for factor structure, principal component analysis (PCA) showed good loading values for most of the items in the instrument.

    Conclusion: The translated version of the Malay AQ-Child for screening ASD can further facilitate the process of surveillance and early intervention for children who need it.

  4. Paramasivan T, Md Amin R, Zhao S, Wang T, Roseli N, Li D, et al.
    Environ Sci Pollut Res Int, 2025 Jan;32(2):587-602.
    PMID: 39695036 DOI: 10.1007/s11356-024-35711-7
    Estuarine fronts are formed due to sharp density discontinuities resulting from the convergence of different water masses. This study, conducted in May and August of 2022 during the southwest monsoon season, focuses on assessing the role of estuarine fronts at Kuala Terengganu estuary in the accumulation of microplastics in surface seawater. The Terengganu River basin area covers approximately 4600 km2 and consists of two main tributaries that drain into the Kuala Terengganu estuary. Microplastic samples were collected from three areas, the plume, front, and shelf, utilizing two methods: manta net (> 350 µm) and bucket-water sampling (> 20 µm). Results indicate that the estuarine front consistently exhibited higher microplastic concentrations than the plume and shelf regions throughout the study period, with bucket-water sampling contributing significantly to the abundance. Specifically, peak concentrations occurred during the ebb tide at the frontal region in both months, reaching 5761.703 particles m-3 and 12,687.437 particles m-3, respectively. The microplastics, predominantly transparent fibers smaller than 1000 µm, mostly showed signs of oxidative and mechanical weathering through SEM-EDS analysis, providing insights into their fate in estuarine surface waters. FTIR spectroscopy revealed polypropylene, polyethylene, and polyamide as the dominant polymers. These findings establish a baseline for microplastic abundance at the estuarine front of the Kuala Terengganu estuary and may inform future strategies for mitigating and recovering microplastic contamination in aquatic environments.
  5. Md Khir NF, Ya'cob Z, Mansor MS, Ismail NA, Daud UNS, Sahimin N
    Trop Biomed, 2024 Dec 01;41(4):438-440.
    PMID: 39876500 DOI: 10.47665/tb.41.4.003
    Migratory birds are potential vectors transmitting zoonotic parasites, bacteria, and viruses that can significantly affect human health. Over 60 diseases have been identified to be associated with these birds. Given their close proximity to human habitation, the potential transmission of zoonotic diseases is a major concern for the public. In this study, we aimed to assess the prevalence of intestinal parasites in the droppings of the Barn Swallow, Hirundo rustica, a bird species that annually migrates to Malaysia. Eighty droppings from H. rustica were collected; 13.75% of the samples were found to be positive for various parasites, including Ascaridia galli (8.75%), Eimeria sp. (2.5%), Hymenolepis spp. (1.25%), and Toxocara spp. (1.25%). These findings offer valuable insights into the current prevalence of bird parasitic infections. They can serve as a crucial resource for implementing effective control measures, devising prevention strategies, and predicting future outbreaks of parasite infections transmitted by birds.
  6. Ang ZY, Kong YL, Md Nesran ZN, Lee SWH
    BJU Int, 2025 Feb;135(2):222-234.
    PMID: 39402746 DOI: 10.1111/bju.16545
    OBJECTIVE: To review and compare the efficacy of different delivery modes of decision aids (DAs), including computer-based, print-based, multimedia-based, video-based, and website-based on decision-making outcomes for prostate cancer screening compared to usual care (UC) and among the delivery modes.

    METHODS: PubMed, the Excerpta Medica dataBASE (EMBASE), Cumulative Index to Nursing and Allied Health Literature (CINAHL), Google Advanced Search, and Turning Research Into Practice (TRIP) Database were systematically searched from inception to August 2023. The primary outcomes were knowledge level, knowledge scores, participation in shared decision-making (SDM), decisional conflict, and preference for SDM participation. Secondary outcomes were the proportion of subjects who underwent screening (actual screening utilisation) and the proportion of subjects who intended to be screened (intention to undergo screening). Network and pairwise meta-analyses were performed using random-effects models.

    RESULTS: Seven systematic reviews were included. Network meta-analysis found that multimedia (relative risk [RR] 1.51, 95% confidence interval [CI] 1.02-2.24), print (RR 1.82, 95% CI 1.23-2.69), and website-based (RR 1.99, 95% CI 1.32-3.01) DAs significantly increased participation in SDM compared to the computer-based DA. There was a significant reduction in the actual screening utilisation in the computer DA arm compared to the other delivery modes. No significant differences between all delivery modes were noted on knowledge levels, knowledge scores, decisional conflict, preference for SDM participation, and intention to undergo screening. The highest mean surface under the cumulative ranking curve for all primary outcomes showed that website-based was the most effective delivery mode, followed by print-based DA. The pairwise meta-analysis showed a significant increase in participants' knowledge level, knowledge scores, a reduced intention to undergo screening and actual screening utilisation compared to UC.

    CONCLUSIONS: The findings suggest that different types of DAs have varying levels of effectiveness in increasing knowledge level, knowledge scores, participation in SDM, and influencing screening behaviours. While website-based DA appeared the most effective, employing the print-based DA could be a practical solution in settings with limited resources.

  7. Sulwarajan K, Jaafar Z, Md Sari NA, Hamzah SH, Yusop FD, Hamid S, et al.
    Patient Educ Couns, 2025 Feb;131:108580.
    PMID: 39644532 DOI: 10.1016/j.pec.2024.108580
    OBJECTIVE: Exercise adherence and motivation can benefit from technology, but the specific factors influencing these improvements remain unclear. This scoping review aimed to outline the types and features of technology used in exercise prescription within healthcare, focusing on enhancing users' adherence to exercise.

    METHODS: A comprehensive systematic literature search was conducted from January 2000 to February 2023 across Web of Science, Google Scholar, and MEDLINE via PubMed. The review sought studies on technology-based exercise prescriptions for adults ≥ 18 years, evaluating features that enhance motivation or adherence.

    RESULTS: Out of 1724 articles reviewed, 45 met the criteria, demonstrating that mobile applications, exercise gaming, wearable trackers, and websites are commonly used technologies for prescribing exercise in healthcare setting. Common features, special features, and motivating features were found to be important in all technology-based exercise tools to enhance users' adherence and motivation.

    CONCLUSION: This review provides a comprehensive overview of the technological types and approaches that can support and improve exercise adherence among health service users. The findings also emphasize on specific features that can address current gaps in technology-driven fitness interventions.

    PRACTICE IMPLICATIONS: Healthcare professionals should make informed decisions when recommending these tools to health service users, since technology does play a significant role in promoting long-term adherence to exercise programs.

  8. Guad RM, Ng KP, Lim SK, Hirayama K, Eng HS, Wan Md Adnan WAH
    Ann Acad Med Singap, 2019 Dec;48(12):403-411.
    PMID: 32112065
    INTRODUCTION: Studies have shown that a compatible human leukocyte antigen (HLA) match can confer a favourable effect on graft outcomes. We examined the outcomes of HLA matching in renal transplant donors in Malaysia.

    MATERIALS AND METHODS: A total of 140 patients who had compatible ABO blood type with negative T-cell lymphocytotoxicity crossmatch were included in the study and 25% of them were spousal transplant donors. No remarkable differences in acute rejection rate, graft survival, patient survival and serum creatinine level were observed between the spousal and living-related donor groups.

    RESULTS: The spousal donor group had a higher degree of HLA mismatch than the living-related donor group. HLA-A mismatch was associated with increased rejection risk at 6 months (odds ratio [OR], 2.75; P = 0.04), 1 year (OR, 2.54; P = 0.03) and 3 years (OR, 3.69; P = 0.001). It was also observed in the deleterious effects of HLA-B and HLA-DQ loci when the number of antigen mismatches increased. The risk was 7 times higher in patients with ≥1 mismatch at HLA-A, HLA-B and HLA-DR loci than those who did not have a mismatch at these loci at 6 months (P = 0.01), 1 year (P = 0.03) and 3 years (P = 0.003).

    CONCLUSION: A good match for HLA-A, HLA-B, HLA-DR and HLA-DQ can prevent acute rejection risk in renal transplant patients. Consequently, spousal donor transplants could be a safe intervention in renal patients.

  9. Ab Majid NL, Vanoh D, Zainuddin NZS, Md Hashim MN
    Asia Pac J Clin Nutr, 2024 Jun;33(2):162-175.
    PMID: 38794976 DOI: 10.6133/apjcn.202406_33(2).0003
    BACKGROUND AND OBJECTIVES: Bariatric surgery becomes the final option for managing severe obesity. This study aims to identify the complications, changes in anthropometry, adherence to dietary recommendations, and psychological well-being of post-bariatric surgery patients.

    METHODS AND STUDY DESIGN: An observational study was conducted on 63 post-bariatric surgery patients who had undergone bariatric surgery between two weeks and five years after surgery. The participants were assessed for the complications experienced, current comorbidities, anthropometric changes, dietary intake, and psychological well-being. A three-day, 24-hour diet recall was done to assess the dietary intake of the patients. The mean macronutrient and micronutrient intakes were compared to several available recommendations. The DASS-21 questionnaire was administered to determine the psychological well-being of the participants.

    RESULTS: The most common complications experienced by patients after bariatric surgery were hair loss (50.8%), gastroesophageal reflux disease (GERD) (49.2%), and vomiting (41.3%). There were significant differences in mean weight before (129.5 (33.0) kg/m2) and after (85.0 (32.0) kg/m2) bariatric surgery (p<0.001). The prevalence of clinically severe obesity declined by 55%. Overall, patients had insufficient intake of some nutrients such as protein, fat, calcium, and iron. Majority of the patients experienced a normal level of stress, anxiety, and depression, but some had mild (3.2%), moderate (4.8%), and severe anxiety (1.6%).

    CONCLUSIONS: There were drastic improvements in patients' weight following bariatric surgery. However, there were several complications including nutrient deficiencies. Due to the anatomical changes in the gastrointestinal tract, patients must comply with the dietary and lifestyle changes and follow up with the healthcare professional. A nutrition module will be helpful for patients to prepare for and adapt to the changes after bariatric surgery.

  10. Che Had NH, Alavi K, Md Akhir N, Muhammad Nur IR, Shuhaimi MSZ, Foong HF
    PMID: 36901256 DOI: 10.3390/ijerph20054243
    The phenomenon of ageing may contribute to the rise of the dependent population. Due to the obstacles and difficulties they confront, the elderly's mobility decreases significantly. The aim of this article is to identify factors associated with mobility barriers in older adults. The method employed is an examination of articles published between 2011 and 2022 to identify common themes in previous studies. Four search engines were being used, and 32 articles have been included. This study demonstrated that health is a major factor associated with decreased mobility. This review identified four types of barriers which are health, built environment, socio-economic background and social relation change. This review could help policy makers and gerontologist in identifying solutions to resolve the mobility issues in older people.
  11. Almalbis CA, Md Redzuan A, Andrada CP, Gonzaga NA, Mohd Saffian S
    J Thromb Haemost, 2025 Jan 09.
    PMID: 39798921 DOI: 10.1016/j.jtha.2024.12.032
    BACKGROUND: Apixaban and rivaroxaban are activated factor X (FXa) inhibitors commonly used for treatment of venous thromboembolism and stroke prevention in patients with atrial fibrillation. While routine monitoring of their concentrations is not recommended, but it may be beneficial in certain situations. Expected peak and trough concentrations remain poorly understood, with most data derived from small studies.

    OBJECTIVES: To establish the average peak and trough concentrations of apixaban and rivaroxaban from real-world studies.

    METHODS: PubMed, Scopus, and Web of Science were searched until October 2023 for observational studies reporting apixaban and rivaroxaban concentrations. Meta-regression was used to examine factors influencing these concentrations.

    RESULTS: Sixteen studies involving 1054 apixaban and 1321 rivaroxaban patients were pooled using random-effects model. Mean apixaban peak concentrations were 157 ng/mL (95% CI, 127-187) for 2.5 mg and 228 ng/mL (95% CI, 204-252) for 5 mg, with trough concentrations of 77 ng/mL (95% CI, 56-98) and 113 ng/mL (95% CI, 101-124), respectively. Mean rivaroxaban peak concentrations were 168 ng/mL (95% CI, 104-232) for 10 mg, 225 ng/mL (95% CI, 192-257) for 15 mg, and 229 ng/mL (95% CI, 193-264) for 20 mg, with trough concentrations of 23 ng/mL (95% CI, 13-32), 31 ng/mL (95% CI, 26-36), and 36 ng/mL (95% CI, 25-47), respectively. Meta-regression revealed age and creatinine clearance correlated with apixaban peak concentrations. Creatinine clearance correlated with apixaban and rivaroxaban trough concentrations.

    CONCLUSION: The pooled mean concentrations align with expected concentration ranges reported in different pharmacokinetic studies.

  12. Mozafari M, Md Hashim SN, Ahmad Amin Noordin KB, Zainal SA, Azlina A
    Cureus, 2024 Dec;16(12):e75844.
    PMID: 39822413 DOI: 10.7759/cureus.75844
    The nuclear factor of activated T cells (NFAT) is a key player in the NFAT pathway, regulating various cellular processes physiologically and pathologically. NFAT signaling is implicated in developing multiple diseases, such as cancer progression, that are associated with angiogenesis. Despite numerous studies on NFAT, there is still a dearth of information on the proteins and signaling pathway compared to other established pathways. With five NFAT proteins in the spotlight, this review aims to update the understanding of their roles and signaling by analyzing the most recent studies on the NFAT pathway. The recent insights into NFAT proteins and their association with diseases enhance our understanding of these proteins and open the possibility of developing therapeutic strategies for such diseases.
  13. bin Sabir Husin Athar PP, bte Ahmad Norhan N, bin Saim L, bin Md Rose I, bte Ramli R
    Ann Acad Med Singap, 2008 Sep;37(9):788-3.
    PMID: 18989497
    INTRODUCTION: Metastatic adenocarcinoma from the gastrointestinal tract to the sinonasal tract is rare. The histological morphology of this lesion is indistinguishable from the colonic variant of primary sinus adenocarcinoma or intestinal-type adenocarcinoma (ITAC).

    CLINICAL PICTURE: This is a report of a case of metastatic adenocarcinoma of colorectal origin to the paranasal sinuses in a 52-year-old female who was previously treated for adenocarcinoma of the sigmoid colon. A histologic study of the surgical specimen from the sinonasal cavity demonstrated a tumour identical to the patient's prior primary tumour of the colon. The sinonasal neoplastic tissue showed marked positivity for carcinoembryonic antigen and expressed cytokeratin 20, which differentiates metastatic colonic adenocarcinoma from ITAC.

    TREATMENT/OUTCOME: The patient received palliative radiation but died 3 months after the diagnosis.

    CONCLUSION: Distinguishing metastatic adenocarcinoma from gastrointestinal tract from ITAC can be difficult. In view of the resemblance, immunohistochemical staining can help in differentiating them. It is important to recognise these as metastatic lesions as the treatment is mainly palliative.

  14. Baderol Allam FN, Ab Hamid MR, Buhari SS, Md Noor H
    J Med Internet Res, 2021 Mar 15;23(3):e22465.
    PMID: 33720036 DOI: 10.2196/22465
    BACKGROUND: Hypertension is the root cause of many chronic diseases. Lifestyle changes (ie, dietary alterations and physical activity) are seen to be an important step in the prevention and treatment of hypertension. Educating people through web-based interventional programs could offer an effective solution and help these patients with hypertension in the existing health care scenario.

    OBJECTIVE: In this study, the researchers conducted a scoping literature review of the web-based dietary changes and physical activity-related intervention programs designed for the patients with hypertension and identified the methodologies, effectiveness, protocols, and theories, which could affect and improve existing clinical activities.

    METHODS: This review followed the scoping review methodology to identify and process the peer-reviewed studies published between 2010 and 2020. The literature searches were conducted on the following electronic databases: PubMed, Web of Science, MEDLINE (Medical Literature Analysis and Retrieval System Online), ScienceDirect, Scopus, and Google Scholar. By using relevant search terms, studies were included if they offered information related to the web-based intervention tools, specifically dietary and physical activity intervention for patients with hypertension. Studies written or translated in English language and published within the date range (January 2010 to March 2020) were included.

    RESULTS: Overall, 1441 articles were initially identified. The reviewers included 35 articles after removing duplicates and screening titles. Only 21 articles were assessed for full review, and 15 were kept for analysis. The researchers selected 15 web-based intervention articles published on the topic of hypertension from 7 countries. A few of these 15 web-based tools (4, 27%) included more than 3 functions and provided a lot of important information (such as appointments, health records, or viewable care). Several tools were standalone tools (11, 73%), while most of the tools supported communication intervention-related lifestyle or behavioral changes (13, 87%) and medication adherence (6, 40%). It was found that physicians (9, 60%), allied health professionals (5, 33%), and nurses (5, 33%) were the health care providers who generally used these tools for communicating with their patients. More than half of the above tools (10, 67%) were assessed by different researchers in randomized controlled trials, while 5 tools (33%) were investigated in nonrandomized studies.

    CONCLUSIONS: We identified many web-based intervention programs for patients with hypertension from the literature databases. The findings indicate that numerous benefits can be derived after using a web-based dietary and physical activity intervention program for hypertension focusing on lifestyle changes. However, developers need to consider the preferences of the patients with regard to the information or the design features while developing or modifying web-based educational websites. These tools could be used for designing a patient-tailored website intervention program that is based on diet and physical activities for patients with hypertension.

  15. Suppiah J, Md Sani SS, Hassan SS, Nadzar NIF, Ibrahim N', Thayan R, et al.
    Virus Genes, 2025 Feb;61(1):26-37.
    PMID: 39397194 DOI: 10.1007/s11262-024-02114-2
    Dengue virus hijacks host cell mechanisms and immune responses in order to replicate efficiently. The interaction between the host and the virus affects the host's gene expression, which remains largely unexplored. This pilot study aimed to profile the host transcriptome as a potential strategy for identifying specific biomarkers for dengue prediction and detection. High-throughput RNA sequencing (RNA-seq) was employed to generate host transcriptome profiles in 16 dengue patients and 10 healthy controls. Differentially expressed genes (DEGs) were identified in patients with severe dengue and those with dengue with warning signs compared to healthy individuals. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to elucidate the functions of upregulated and downregulated genes. Compared to healthy controls, 6466 genes were significantly differentially expressed (p 
  16. Khairulmunir M, Gani M, Mohd-Ridwan AR, Karuppannan KV, Abdul-Latiff MAB, Md-Zain BM
    Mol Biol Rep, 2024 Jun 14;51(1):742.
    PMID: 38874703 DOI: 10.1007/s11033-024-09642-y
    BACKGROUND: Enrichment activities may influence the microbiomes of captive tigers', affecting their health, digestion, and behavior. Currently, there are few studies that address the impact of enrichment activity on tigers' health. This study aimed to determine the diversity of the gut microbiome in captive Malayan tigers at Zoo Melaka and Night Safari during the environmental enrichment phase using a metabarcoding approach.

    METHODS AND RESULTS: This study utilized different enrichment activities which catered for food, sensory, and cognitive enrichment. Eleven fresh fecal samples from captive Malayan tigers at Zoo Melaka and Night Safari were collected under different conditions. All samples were extracted and 16S rRNA V3-V4 region amplicon sequencing was used to characterize the gut microbiome of captive Malayan tigers subjected to various enrichment activities. Firmicutes, Actinobacteriota, and Fusobacteriota were the dominant phyla observed in the gut microbiome of captive Malayan tigers during enrichment activities. This study revealed β-diversity significantly varied between normal and enrichment phase, however no significant differences were observed in α-diversity. This study demonstrates that environmental enrichment improves the gut microbiome of Malayan tigers because gut microbes such as Lachnoclostridium, which has anti-inflammatory effects and helps maintain homeostasis, and Romboutsia, which has a probiotic effect on the gut microbiome.

    CONCLUSIONS: This study provides valuable insights into the effects of enrichment activities on the gut microbiome of captive Malayan tigers, offering guidance for enhancing captive management practices aimed at promoting the health and well-being of Malayan tiger in captivity.

  17. Ab Rahman N, Chellapan K, Ong PY, Adnan A, Md Din N
    Retina, 2025 Feb 01;45(2):310-317.
    PMID: 39442016 DOI: 10.1097/IAE.0000000000004297
    PURPOSE: To evaluate systemic vascular fitness measured by finger photoplethysmography in diabetic retinopathy (DR).

    METHODS: This was a cross-sectional observational study on patients with Type II diabetes mellitus from October 2020 to May 2021. Data collected include systolic/diastolic blood pressure, visual acuity, glycated hemoglobin, and central macular thickness. Diabetic retinopathy severity was categorized using the Early Treatment Diabetic Retinopathy Study classification. Photoplethysmography signals were acquired using pulse-oximeter modules (OEM-60; Dolphin Medical, Inc) measured for 90 seconds at 275 Hz sampling rate and 16-bit resolution, which records photoplethysmography fitness index, vascular risk prediction index, and vascular age.

    RESULTS: One hundred and forty-one patients were equally distributed into six DR categories. Mean age was 58.8 ± 9.9 years, with female-to-male ratio of 1.27. There were significant differences in mean systolic (125.5 ± 10.0 mmHg, P = 0.007) and diastolic blood pressure (80.0 ± 8.8 mmHg), mean glycated hemoglobin (7.6 ± 1.9%, P = 0.005), median log unit of minimal angle of resolution (0.3, interquartile range: 0.2-0.5, P < 0.001), and central macular thickness ( P = 0.003) across DR severity. Significant differences were also seen in photoplethysmography fitness index ( P = 0.001), vascular risk prediction index ( P < 0.001), and vascular age ( P = 0.001), with poorer values in severe compared with mild/moderate DR. After adjusting for age, blood pressure, and glycated hemoglobin, photoplethysmography fitness reduces by 3.3% (regression coefficient, b = -3.27, P < 0.001), vascular age increases by 2.5 years ( b = 2.54, P = 0.002), and vascular risk prediction index increases by 3.1 ( b = 3.08, P < 0.001) with every DR worsening.

    CONCLUSION: More severe DR stages were associated with poorer photoplethysmography vascular markers.

  18. Romaino SM, Fazly-Ann ZA, Loo SS, Hafiz MM, Hafiz MD, Iswadi MI, et al.
    Genet. Mol. Res., 2014;13(1):406-14.
    PMID: 24535867 DOI: 10.4238/2014.January.21.8
    Mitochondrial DNA (mtDNA) is a useful genetic marker that can be used for species identification. The cytochrome b (Cyt b) gene is a suitable mtDNA candidate gene for use in phylogenetic analyses due to its sequence variability, which makes it appropriate for comparisons at the subspecies, species, and genus levels. This study was conducted to develop a rapid molecular method for species identification of Malayan gaur (Bos gaurus hubbacki), Kedah-Kelantan (KK) (Bos indicus), and Bali (Bos javanicus) cattle in Malaysia. DNA was extracted from blood samples of 8 Malayan gaurs, 30 KK, and 28 Bali cattle. A set of both specific and universal primers for the Cyt b gene were used in PCR amplification. DNA sequences obtained were then analyzed using BioEdit and Restriction Mapper softwares. The PCR products obtained from Cyt b gene amplification were then subjected to restriction enzyme digestion. The amplification, using both specific and universal primers, produced a 154- and a 603-bp fragment, respectively, in all three species. Two restriction enzymes, NlaIV and SspI, were used to obtain specific restriction profiles that allowed direct identification of Malayan gaur, KK, and Bali cattle. Our findings indicate that all three species can be identified separately using a combination of universal primers and the restriction enzyme SspI.
  19. Mimi O, Tong SF, Nordin S, Teng CL, Khoo EM, Abdul-Rahman A, et al.
    Malays Fam Physician, 2011;6(1):19-25.
    PMID: 25606215 MyJurnal
    OBJECTIVES: To compare the morbidity patterns in public and private primary care clinics; determine patients' reasons for encounter (RFE) and diagnoses using the ICPC-2, and compare ten commonest diagnoses and RFEs.
    METHODS: A cross-sectional study on randomly selected clinics was conducted nationwide. Doctors completed the Patient Encounter Record (PER) for systematically selected encounters for a week.
    RESULTS: Response rate was 82.0% (public clinic) and 33% (private clinic) with 4262 encounters and 7280 RFE. Overall, the three commonest disease categories encountered were respiratory (37.2%), general and unspecified (29.5%), and cardiovascular diseases (22.2%). Public and private clinics handled 27% versus 50% acute cases and 20.0% versus 3.1% chronic cases i.e. 33.7 and 5.6 chronic diseases per 100 RFE respectively.
    CONCLUSION: Doctors in public clinics saw more chronic and complex diseases as well as pregnancy related complaints and follow-up cases while in private clinics more acute and minor illnesses were seen. Health services should be integrated and support given to co-manage chronic diseases in both sectors.
    KEYWORDS: Malaysia; Primary practice; delivery of health care; morbidity pattern; reasons for encounter
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