Displaying publications 21 - 40 of 68 in total

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  1. In vitro antimicrobial activity against Pseudomonas aeruginosa and acute oral toxicity of marine algae Gracilaria changii
    Sasidharan S, Darah I, Noordin MK
    N Biotechnol, 2010 Sep 30;27(4):390-6.
    PMID: 20170762 DOI: 10.1016/j.nbt.2010.02.002
    Methanol extract of the Gracilaria changii has been screened for antimicrobial activity against Pseudomonas aeruginosa. Antimicrobial activities were carried out using disc diffusion assay and broth dilution method against P. aeruginosa. The methanol extract of G. changii showed a good antimicrobial activity against P. aeruginosa with MIC (Minimum Inhibitory Concentration) value of 6.25mg/ml. Exposure of P. aeruginosa cells to 6.25mg/ml of methanol extract of G. changii resulted in complete inhibition of the bacterial cells. The main abnormalities noted via SEM and TEM studies were the alterations in morphology and cytology of the bacterial cells. The main reason for this deterioration was discussed. The effect of the methanol extract on the growth profile for the bacteria was also done and confirmed the bactericidal effect of the G. changii methanol extract on P. aeruginosa by changing the normal growth profile of P. aeruginosa. In an acute toxicity study using mice, the median lethal dose (LD(50)) of the extract was greater than 2000 mg/kg, and we found no pathological changes in macroscopic examination by necropsy of mice treated with extract. We conclude that G. changii might be safely used as an antimicrobial agent.
    Matched MeSH terms: Complex Mixtures/toxicity
  2. Fulltext Authentication of Nigella sativa seed oil in binary and ternary mixtures with corn oil and soybean oil using FTIR spectroscopy coupled with partial least square
    Rohman A, Ariani R
    ScientificWorldJournal, 2013;2013:740142.
    PMID: 24319381 DOI: 10.1155/2013/740142
    Fourier transform infrared spectroscopy (FTIR) combined with multivariate calibration of partial least square (PLS) was developed and optimized for the analysis of Nigella seed oil (NSO) in binary and ternary mixtures with corn oil (CO) and soybean oil (SO). Based on PLS modeling performed, quantitative analysis of NSO in binary mixtures with CO carried out using the second derivative FTIR spectra at combined frequencies of 2977-3028, 1666-1739, and 740-1446 cm(-1) revealed the highest value of coefficient of determination (R (2), 0.9984) and the lowest value of root mean square error of calibration (RMSEC, 1.34% v/v). NSO in binary mixtures with SO is successfully determined at the combined frequencies of 2985-3024 and 752-1755 cm(-1) using the first derivative FTIR spectra with R (2) and RMSEC values of 0.9970 and 0.47% v/v, respectively. Meanwhile, the second derivative FTIR spectra at the combined frequencies of 2977-3028 cm(-1), 1666-1739 cm(-1), and 740-1446 cm(-1) were selected for quantitative analysis of NSO in ternary mixture with CO and SO with R (2) and RMSEC values of 0.9993 and 0.86% v/v, respectively. The results showed that FTIR spectrophotometry is an accurate technique for the quantitative analysis of NSO in binary and ternary mixtures with CO and SO.
    Matched MeSH terms: Complex Mixtures/analysis
  3. Genotoxic effect of Catha edulis (khat) crude extract after sub-chronic administration in rats
    Al-Zubairi A, Ismail P, Pei Pei C, Rahmat A
    Environ Toxicol Pharmacol, 2008 May;25(3):298-303.
    PMID: 21783866 DOI: 10.1016/j.etap.2007.10.032
    The aim of this study was to evaluate the genotoxic effects of a crude extract of khat (Catha edulis, Forsk) leaves in rats. Two groups were fed khat crude extract, 1000 and 2000mg/kg body weight, for 90 days and were compared with a control group. The alkaline (pH>13) version of comet assay was used in this study. However, no previous published work has been undertaken and showed the effect of khat on DNA migration in the comet assay. To compare the comet assay results with another genetic endpoint, blood samples were analyzed for chromosomal aberrations. These results showed no DNA damage detected using comet assay in both the khat treated groups, while the results of chromosomal aberrations assay showed a significant increase (P<0.05) in the 2000mg/kg body weight treated group compared to the control group.
    Matched MeSH terms: Complex Mixtures
  4. Fulltext Classification of different pineapple varieties grown in Malaysia based on volatile fingerprinting and sensory analysis
    Lasekan O, Hussein FK
    Chem Cent J, 2018 Dec 19;12(1):140.
    PMID: 30569201 DOI: 10.1186/s13065-018-0505-3
    BACKGROUND: Pineapple is highly relished for its attractive sweet flavour and it is widely consumed in both fresh and canned forms. Pineapple flavour is a blend of a number of volatile and non-volatile compounds that are present in small amounts and in complex mixtures. The aroma compounds composition may be used for purposes of quality control as well as for authentication and classification of pineapple varieties.

    RESULTS: The key volatile compounds and aroma profile of six pineapple varieties grown in Malaysia were investigated by gas chromatography-olfactometry (GC-O), gas-chromatography-mass spectrometry and qualitative descriptive sensory analysis. A total of 59 compounds were determined by GC-O and aroma extract dilution analysis. Among these compounds, methyl-2-methylbutanoate, methyl hexanoate, methyl-3-(methylthiol)-propanoate, methyl octanoate, 2,5-dimethyl-4-methoxy-3(2H)-furanone, δ-octalactone, 2-methoxy-4-vinyl phenol, and δ-undecalactone contributed greatly to the aroma quality of the pineapple varieties, due to their high flavour dilution factor. The aroma of the pineapples was described by seven sensory terms as sweet, floral, fruity, fresh, green, woody and apple-like.

    CONCLUSION: Inter-relationship between the aroma-active compounds and the pineapples revealed that 'Moris' and 'MD2' covaried majorly with the fruity esters, and the other varieties correlated with lesser numbers of the fruity esters. Hierarchical cluster analysis (HCA) was used to establish similarities among the pineapples and the results revealed three main groups of pineapples.

    Matched MeSH terms: Complex Mixtures
  5. Fulltext Phytochemical screening and larvicidal activity of murraya koenigii leaves ethanolic extract against aedes aegypti
    Norazsida Binti Ramli, Nur Elia Amira Mohd Roslin, Deny Susanti
    International Journal of Allied Health Sciences, 2018;2(1):129-139.
    MyJurnal
    World Health Organization (WHO) estimated over 100 million dengue infections to happen annually worldwide involving more than 2.5 billion people. Temephos or abate is a larvicide that has been used in vector control to eradicate mosquito larvae. Though practically low risk, there had been resistance problem reported with continuous use. This study seeks to find an effective and safer alternative to abate by assessing the use of ethanolic extract of Murraya koenigii leaves as larvicidal agent against Aedes aegypti. M.koenigii leaves were macerated for 3 days with absolute ethanol and evaporated using rotary vapor to produce the crude extract. The crude extract was subjected to phytochemical screening using standard qualitative method. For bioassay, the crude
    extract underwent a serial dilution to produce 3 concentrations of 100 ppm (C1), 50 ppm (C2) and 10 ppm (C3) with abate and absolute ethanol as negative and positive control respectively. Bioassay for larvicidal effect was conducted in accordance to WHO standard method. Phytochemical screening of ethanolic extract of M. koenigii leaves revealed the presence of alkaloid, steroid and saponin. The bioassay shows that after 24 hours, the mortality rate of C1, C2 and C3 larvae were 100%, 38% and 0% and when further extended to 48 hours, the rate increased to 100% and 46% for C2 and C3 respectively. The LC50 and LC99 post 24 hours were 54.489 ppm and 93.961 ppm respectively whilst at post 48 hours, the LC50 and LC99 were 10.263 ppm and 16.176 ppm respectively. The results show that up to 48 hours duration of exposure, the mortality
    rate increase whilst the lethal concentration (LC50 and LC99) decreases. Upon examination on larvae deformities at post 24 and 48 hours, all test concentrations and negative control exhibit normal morphology. Positive control, however, exhibit deformities characterized by twisted and fragmented insides. When statistically analyzed, C1 larvicidal activity was proven comparable with abate at 24 hours while C2 needed 48 hours exposure to be on par. Based on the results, it could be argued that the ethanolic extract of M.koenigii leaves does hold promising value to be further developed as larvicidal.
    Matched MeSH terms: Complex Mixtures
  6. Fulltext The antimicrobial activities of fig (Ficus Carica L.) leaves extract against staphylococcus aureus and escherechia coli
    Amirah Nadiah Ali, Mohd Syahmi Salleh, Ahmad Fahmi Harun, Muhamad Ashraf Rostam
    International Journal of Allied Health Sciences, 2018;2(1):273-284.
    MyJurnal
    Increasing risk of antibiotic resistance of pathogenic bacteria has led to the exploration of alternative antibiotics derived from leaves of medicinal plants such as the fig (Ficus carica L.). The aim of this study was to determine the antimicrobial activity of the methanolic extract of fig leaves grown under Malaysian tropical environment against pathogenic bacteria linked to antibiotic resistance namely the Staphylococcus aureus and Escherechia coli. The methanolic extraction was performed by using soxhlet apparatus. The disc diffusion method was used to measure inhibition zone diameter on the Mueller-Hinton agar plate. Staphylococcus aureus displayed the highest diameter of inhibition zone against the extract at concentration of 900 mg/ml whilst Escherechia coli displayed the highest diameter of inhibition zone against both the 100% crude extract and 700 mg/ml, respectively. This study therefore highlighted the potential of developing alternative antibiotics derived from the methalonic extract of locally grown fig plant.
    Matched MeSH terms: Complex Mixtures
  7. Immunosuppressive and hepatoprotective potential of Sarcococca saligna and its biomarker components
    Ali H, Musharraf SG, Iqbal N, Adhikari A, Abdalla OM, Ahmed Mesaik M, et al.
    Int Immunopharmacol, 2015 Sep;28(1):235-43.
    PMID: 26093268 DOI: 10.1016/j.intimp.2015.06.009
    Sarcococca saligna methanolic extract, fractions and isolated pure compounds saracocine (1), saracodine (2), pachyximine-A (3) and terminaline (4) were found to possess potent immunosuppressive activities. The fractions and compounds were tested in-vitro for their effects on human T-cell proliferation, and cytokine (IL-2) production. All the fractions, sub-fractions and purified compounds showed significant suppressive effect on IL-2 production in a dose-dependent manner. They also exhibited a suppressive effect on the phytohemagglutinin-stimulated T-cell proliferation. None of the extracts and purified compounds showed any cytotoxicity effects on the 3T3 mice fibroblast cell line. The crude extract, DCM fraction (pH9), DCM fractions (pH7) and one of the steroidal alkaloids (terminaline) were checked in-vivo for their hepato-protective potential against CCl4-induced liver injury. In in-vivo experiments, the basic and neutral DCM fractions and terminaline (4) significantly reduced inflammation in the liver. DCM fraction (pH9), DCM fractions (pH7) and compound 4 reduced the serum enzyme levels (ALT, AST, and ALP) down to control levels despite CCl4 treatment. They also reduced the CCl4-induced damaged area to almost zero as assessed by histopathology. The pale necrotic areas and mixed inflammatory infiltrate which are seen after CCl4 treatment were absent in the cases of basic, neutral fractions and terminaline treatment. These hepato-protective effects were better than the positive control silymarin. Our results suggest the therapeutic effect of S. saligna extract, fractions and bioactive steroidal alkaloids against CCl4-induced liver injury in vivo and their immunosuppressive function in vitro.
    Matched MeSH terms: Complex Mixtures
  8. Fulltext PHYTOCHEMICAL SCREENING AND EVALUATION OF THE ENZYMATIC ANTIOXIDANT ACTIVITIES OF SELECTED Mangifera spp. LEAVES (ANACARDIACEAE)
    MISA HARSRINURI RAIHANI SAIRUN, NORHAYATI YUSUF, NORHAYATI YUSUF, NURUL HUDA ABDUL WAHAB, NURUL HUDA ABDUL WAHAB
    UMT Journal of Undergraduate Research, 2020;2(4):1-8.
    MyJurnal
    A study was conducted on the chemical and biological properties of three different species of Mangifera i.e. Mangifera pajang, M. indica L., and M. kemanga leaves obtained from Pitas, Sabah. The aims of this study were to determine the presence of secondary metabolites as well as the antioxidative activities especially the catalase (CAT) and guiacol peroxidase specific activities (gPOD) in the leaves part of these three species. The extraction of these samples was carried out using three different polarities of solvents: hexane, ethyl acetate, and methanol. The total percentage of the crude extract of is 7.30% for M. pajang, 12.87% for M. indica and 7.98% for M. kemanga. Phytochemical screening was performed with various tests for each of the crude extracts. The results showed that these three species gave positive results for alkaloids, saponins, flavonoids, phenols, carbohydrates, phytosterols, and tannins metabolites. Based on the tests, CAT specific activities were significantly higher in the leaves of M. pajang with 4.35 ± 1.18 units/mg protein compared to M. indica L. and M. kemanga. The guaiacol peroxidase (gPOD) specific activities showed that M. indica L. has the highest activity with the value of 0.0047 ± 0.0004 units/mg protein.
    Matched MeSH terms: Complex Mixtures
  9. Fulltext Characteristics of threadfin bream (nemipterus japonicas) hydrolysate produced using bilimbi (averrhoa bilimbi l.) protease and alcalase
    Normah, I., Nur Anati, J.
    International Food Research Journal, 2015;22(6):2259-2266.
    MyJurnal
    Threadfin bream (Nemipterus japonicas) muscle was hydrolysed using protease extracted from
    bilimbi (Averrhoa bilimbi L.) fruit. This study was performed in order to compare the efficiency of bilimbi protease in producing threadfin bream protein hydrolysate with the commercial protease; alcalase 2.4 L. Initially, protease was extracted and then purified using 40% ammonium sulfate precipitation method. The proteolytic activity of the crude extract and purified protease was determined. Precipitation using 40% ammonium sulfate resulted in bilimbi protease specific activity of 2.36 U/mg and 23.13% recovery. Threadfin bream hydrolysate was prepared based on the pH-stat method by hydrolysis for 2 hrs. Hydrolysis using bilimbi protease produced 34.76% degree of hydrolysis (DH) and 3.75% yield while hydrolysis using alcalase resulted in 86.6% DH with 22.78% yield. Alcalase hydrolysate showed higher solubility than bilimbi protease hydrolysate at pH 7 with 70.87 and 32.16% solubility, respectively. Results also showed that protein content of threadfin bream hydrolysate produced using alcalase was higher (86.86%) than those produced using bilimbi protease (22.12%). However, both hydrolysates showed low moisture content between 3.93 to 7.00%. The molecular weight distribution analysis using SDS–PAGE indicated the distribution of smaller peptides especially in alcalase hydrolysate. Overall, the results showed that alcalase is more efficient enzyme choice than bilimbi protease for preparing threadfin bream hydrolysates. However, both hydrolysates could play an important role thus contribute to the food industry.
    Matched MeSH terms: Complex Mixtures
  10. Fulltext Characterization and purification of protease extracted from two maturity stages of ‘noni’ (Morinda citrifolia L.) fruit
    Normah Ismail, Nurulain Abd Razak
    Scientific Research Journal, 2014;11(2):0-0.
    MyJurnal
    Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5). The crude extract was partially purified by acetone precipitation method followed by dialysis, gel filtration chromatography and freeze drying. Protein concentrations, proteolytic activity, molecular weight distribution, pH stability, temperature stability and storage efficiency of the resulting protease were evaluated. The unripe and ripe noni fruit contains 0.65 and 0.35% protein, respectively. Molecular weight of the proteases from both stages ranged approximately between 3 to 28 kDa based on the SDS-PAGE results. The optimum activity were at pH 7s and 6, temperatures of 40 and 50°C, respectively for proteases obtained from the unripe and ripe fruit. Analysis from the freeze dried protease indicated that protease from ripe noni fruits had higher protein concentration and specific activity compared to those from unripe fruit. However, it is more sensitive to pH and temperature and less stable during storage as it shows lower proteolytic activity compared to protease from unripe fruit. Based on its high proteolytic activity reaching up to 70.31 U/mg and storage stability (30% lost of activity), noni fruit could be an alternative source of plant protease.
    Matched MeSH terms: Complex Mixtures
  11. Fulltext In vitro antiplasmodial properties of selected plants of Sabah
    Khozirah, S., Rain, A. Noor, Siti Najila, M.J., Rohaya, C., Rosilawati, M., Nuziah, H., et al.
    Pertanika Journal of Science & Technology, 2011;19(1):11-17.
    MyJurnal
    The antiplasmodial activity of the crude extracts of thirty plant species collected from Sabah was evaluated using chloroquine-sensitive strain (D10) and chloroquine-resistant strain (Gombak A) of Plasmodium falciparum. Significant activities were observed for the bark extract of Polyalthia insignis (IC50 3.89 and 11.89 mg/ml against Gombak A and D10, respectively), the leaf extracts of Kopsia dasyrachis (4.62 mg/ml against Gombak A) and Litsea elliptibacea (IC50 8.88 mg/ml against Gombak A), as well as the leaf and bark extracts of Neouvaria acuminatissima (IC50 6.90-10.08 and 0.69 mg/ml against Gombak A and D10, respectively), and the bark extract of Polyalthia microtus(IC50 9.0 and 12.12 mg/ml against Gombak A and D10, respectively).
    Matched MeSH terms: Complex Mixtures
  12. Chemical components of marine sponge derived fungus fusarium proliferatum collected from Pulau Tinggi, Malaysia
    Mosadeghzad Z, Zuriati Zakaria, Asmat A, Gires U, Wickneswari R, Pittayakhajonwut P, et al.
    Sains Malaysiana, 2012;41:333-337.
    Marine fungus Fusarium proliferatum derived from marine sponge collected along Pulau Tinggi, Malaysia was cultivated on Potato Dextrose Broth and incubated for 7 days at 30oC. The liquid cultures were then extracted using ethyl acetate. The crude extract was investigated for its anti-microbial activity and was passed through Sephadex column and the fractions were collected. Reverse phase HPLC was used to monitor the component of crude extract. HPLC guided purification of crude extract resulted in the isolation of linoleic acid, 4-hydroxy phenethyl alcohol, 2,5-furandimethanol and adenosine. Their structures were elucidated by spectroscopic methods.
    Matched MeSH terms: Complex Mixtures
  13. Antioxidant activity of methanol extract of tinospora crispa and tabernaemontana corymbosa
    Heida Nadia Zulkefli, Jamaludin Mohamad, Nurhayati Zainal Abidin
    Sains Malaysiana, 2013;42:697-706.
    Tinospora crispa and Tabernaemontana corymbosa have been used traditionally to treat fever, diabetes, rheumatism and sinusitis. The objective of this study was to evaluate the antioxidant activity of Tinospora crispa and Tabernaemontana corymbosa. The presence of apigenin and magnoflorine was detected using LCMS/MS in Tinospora crispa (Patawali) whereas appararicine, voafinine, conodusarine, conodurine, voacamine and voacangine were detected in Tabernaemontana corymbosa (Susur kelapa) methanol extract. The stem extract of Tinospora crispa showed high antioxidant activity in the following order: DPPH radical scavenging, reducing power and metal chelating assay (98.8%, 0.957, 81.97%) than Tabernaemontana corymbosa of leaves (90.04%, 0.652, 69.64%), stem (82.78%, 0.819, 36.70%) and root extracts (63.25%, 0.469, 51.56%), respectively. The high antioxidant activity in the stem extract of Tinospora crispa is due to the presence of apigenin and magnoflorine. The high antioxidant activity in Tabernaemontana corymbosa extract is due to its high phenol contents. There were significant linear positive correlation (r=0.788, p<0.001, r2=0.621) between the total phenolic content and DPPH free radical scavenging assay in the crude extracts of Tinospora crispa and Tabernaemontana corymbosa. Meanwhile, a significant moderate positive correlation was observed between the total phenolic content and ferric reducing power assay (r= 0.556, p<0.05, r2= 0.309). However, there was no significant difference in the correlation coefficient of total phenolic content and metal chelating assay.
    Matched MeSH terms: Complex Mixtures
  14. Fulltext Effect of dried and extrudate of bitter gourd fruit on epithelial microflora in raw chicken legs meat
    Rukayadi, Y., Abdulkarim, S.M., Sulaiman, R., Abdelkarim, H.
    International Food Research Journal, 2017;24(5):2253-2258.
    MyJurnal
    Plants have been used recently to eliminate bacterial growth in food products. This study was undertaken to test the in vitro sanitizing effect of crude extract from bitter gourd (BG) fruit on the growth of native microorganisms in raw chicken leg meat. Hot air dried BG and extrudate extracts at 1% concentration and exposure times of (5, 10 and 15 min) were used to treat the samples using dilution method. Results showed that BG extrudate had a slightly stronger bactericidal activity against the microflora than the B.G. hot air drying treatment, especially, on E. coli at all exposure time. Overall, there is no significant difference between the treatments; Total Plate Count (TPC), Escherichia coli, Bacillus cereus, Staphylococcus aureus. The best reduction time of microflora by hot air dried extract was at (15 min) except for B. cereus was at (5 min) and for extrudate extract was at (5 min) except for E. coli was at (10 min). In conclusion, bitter gourd extract could be used as an important natural sanitizer for rinsing raw food matrials such chicken meat.
    Matched MeSH terms: Complex Mixtures
  15. Antioxidant and Wound Healing Activity of Polyherbal Fractions of Clinacanthus nutans and Elephantopus scaber
    Aslam MS, Ahmad MS, Mamat AS, Ahmad MZ, Salam F
    Evid Based Complement Alternat Med, 2016;2016:4685246.
    PMID: 27528881 DOI: 10.1155/2016/4685246
    Elephantopus scaber and Clinacanthus nutans are traditionally used as wound healing herb. The objective of the present study is to develop a new polyherbal formulation, by comparison, the herbal combination of Elephantopus scaber and Clinacanthus nutans as an in vitro antioxidant activity with their individual herbal activity followed by fractionation of polyherbal formulation for in vivo wound healing activities and identification of bioactive compounds from their active fractions. Antioxidant activity was performed in vitro by DPPH scavenging antioxidant activity followed by in vivo wound healing activities using excision wound model, incision wound model, and burn wound model. Toxicity of the fractions of the polyherbal formulation was performed by a dermal toxicity test. The result showed that Elephantopus scaber crude extract on the basis of EC50 performs a much faster action (15.67 μg/mL) but with less % inhibition (87.66%) as compared to the combination of the new polyherbal formulation of crude extract (30 μg/mL). The polyherbal formulation has the highest % inhibition (89.49%) at the same dose as compared to Elephantopus scaber (87.66%). In comparison among all crude and fractions of new polyherbal formulation, it was found that the ethyl acetate fraction of polyherbal formulation has the fastest activity (EC50 14.83 μg/mL) with % inhibition (89.28%). Furthermore, during evaluation of wound contraction on excision and incision wound model, ethyl acetate fraction possesses the highest activity with (P < 0.001) and (P < 0.0001), respectively. During burn wound model, aqueous fraction (P < 0.001) possesses the highest activity followed by an ethyl acetate fraction (P < 0.0001). LC-MS analysis discovered the presence of several flavonoid-based compounds that work synergistically with sesquiterpene lactone and other bioactive compounds. In conclusion, flavonoid increases the antioxidant activity that surges the rate of wound contraction and works synergistically with other bioactive compounds.
    Matched MeSH terms: Complex Mixtures
  16. Fulltext Cytotoxic and Antifungal Activities of 5-Hydroxyramulosin, a Compound Produced by an Endophytic Fungus Isolated from Cinnamomum mollisimum
    Santiago C, Fitchett C, Munro MH, Jalil J, Santhanam J
    Evid Based Complement Alternat Med, 2012;2012:689310.
    PMID: 22454674 DOI: 10.1155/2012/689310
    An endophytic fungus isolated from the plant Cinnamomum mollissimum was investigated for the bioactivity of its metabolites. The fungus, similar to a Phoma sp., was cultured in potato dextrose broth for two weeks, followed by extraction with ethyl acetate. The crude extract obtained was fractionated by high-performance liquid chromatography. Both crude extract and fractions were assayed for cytotoxicity against P388 murine leukemic cells and inhibition of bacterial and fungal pathogens. The bioactive extract fraction was purified further and characterized by nuclear magnetic resonance, mass spectral and X-ray crystallography analysis. A polyketide compound, 5-hydroxyramulosin, was identified as the constituent of the bioactive fungal extract fraction. This compound inhibited the fungal pathogen Aspergillus niger (IC(50) 1.56 μg/mL) and was cytotoxic against murine leukemia cells (IC(50) 2.10 μg/mL). 5-Hydroxyramulosin was the major compound produced by the endophytic fungus. This research suggests that fungal endophytes are a good source of bioactive metabolites which have potential applications in medicine.
    Matched MeSH terms: Complex Mixtures
  17. Fulltext Effects of Flower and Fruit Extracts of Melastoma malabathricum Linn. on Growth of Pathogenic Bacteria: Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium
    Che Omar SN, Ong Abdullah J, Khairoji KA, Chin Chin S, Hamid M
    Evid Based Complement Alternat Med, 2013;2013:459089.
    PMID: 23662136 DOI: 10.1155/2013/459089
    Melastoma malabathricum Linn. is a shrub that comes with beautiful pink or purple flowers and has berries-like fruits rich in anthocyanins. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of the M. malabathricum Linn. flower and fruit crude extracts against Listeria monocytogenes IMR L55, Staphylococcus aureus IMR S244, Escherichia coli IMR E30, and Salmonella typhimurium IMR S100 using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). In addition, the growth of Listeria monocytogenes IMR L55 and Staphylococcus aureus IMR S244 grown in medium supplemented with the respective extracts at different temperatures (4°C, 25°C, and 37°C) and pHs (4, 6, 7, and 8) was determined.
    Matched MeSH terms: Complex Mixtures
  18. Fulltext Toxicological Features of Catha edulis (Khat) on Livers and Kidneys of Male and Female Sprague-Dawley Rats: A Subchronic Study
    Alsalahi A, Abdulla MA, Al-Mamary M, Noordin MI, Abdelwahab SI, Alabsi AM, et al.
    Evid Based Complement Alternat Med, 2012;2012:829401.
    PMID: 23259000 DOI: 10.1155/2012/829401
    Hepato- and nephrotoxicity of Khat consumption (Catha edulis Forskal) have been evoked. Therefore, this study was conducted to evaluate such possible hepatorenal toxicity in female and male Sprague-Dawley rats (SD rats) focusing primarily on liver and kidney. In addition, female and male rats were investigated separately. Accordingly, forty-eight SD-rats (100-120 g) were distributed randomly into four groups of males and female (n = 12). Normal controls (NCs) received distilled water, whereas test groups received 500 mg/kg (low dose (LD)), 1000 mg/kg (medium dose (MD)), or 2000 mg/kg (high dose (HD)) of crude extract of Catha edulis orally for 4 weeks. Then, physical, biochemical, hematological, and histological parameters were analyzed. Results in Khat-fed rats showed hepatic enlargement, abnormal findings in serum aspartate aminotransferase (AST), and alkaline phosphatase (ALP) of male and female SD-rats and serum albumin (A) and serum creatinine (Cr) of female as compared to controls. In addition, histopathological abnormalities confirmed hepatic and renal toxicities of Khat that were related to heavy Khat consumption. In summary, Khat could be associated with hepatic hypertrophy and hepatotoxicity in male and female SD-rats and nephrotoxicity only in female SD-rats.
    Matched MeSH terms: Complex Mixtures
  19. Inhibition of Staphylococcus aureus by crude and fractionated extract from lactic acid bacteria
    Wong CB, Khoo BY, Sasidharan S, Piyawattanametha W, Kim SH, Khemthongcharoen N, et al.
    Benef Microbes, 2015 Mar;6(1):129-39.
    PMID: 25213027 DOI: 10.3920/BM2014.0021
    Increasing levels of antibiotic resistance by Staphylococcus aureus have posed a need to search for non-antibiotic alternatives. This study aimed to assess the inhibitory effects of crude and fractionated cell-free supernatants (CFS) of locally isolated lactic acid bacteria (LAB) against a clinical strain of S. aureus. A total of 42 LAB strains were isolated and identified from fresh vegetables, fresh fruits and fermented products prior to evaluation of inhibitory activities. CFS of LAB strains exhibiting a stronger inhibitive effect against S. aureus were fractionated into crude protein, polysaccharide and lipid fractions. Crude protein fractions showed greater inhibition against S. aureus compared to polysaccharide and lipid fractions, with a more prevalent effect from Lactobacillus plantarum 8513 and L. plantarum BT8513. Crude protein, polysaccharide and lipid fractions were also characterised with glycine, mannose and oleic acid being detected as the major component of each fraction, respectively. Scanning electron microscopy revealed roughed and wrinkled membrane morphology of S. aureus upon treatment with crude protein fractions of LAB, suggesting an inhibitory effect via the destruction of cellular membrane. This research illustrated the potential application of fractionated extracts from LAB to inhibit S. aureus for use in the food and health industry.
    Matched MeSH terms: Complex Mixtures/isolation & purification; Complex Mixtures/pharmacology*
  20. Fulltext In vitro antibacterial activity of Quercus infectoria gall extracts against multidrug resistant bacteria
    Wan Nor Amilah WA, Masrah M, Hasmah A, Noor Izani NJ
    Trop Biomed, 2014 Dec;31(4):680-8.
    PMID: 25776593 MyJurnal
    Antimicrobial activities of plants have long been evaluated for their promising use as antimicrobial agent and in minimizing the unwanted resistance effects of microorganisms. The study was conducted to evaluate the antibacterial activity of Quercus infectoria gall crude extracts against multidrug resistant (MDR) bacteria in vitro. The screening test was determined by disc diffusion technique using sterile filter paper discs impregnated with 1 mg/ disc (50 mg/ml) aqueous and ethanol extracts of Q. infectoria galls tested on five selected MDR bacterial strains. The minimum inhibitory concentration (MIC) was determined using the twofold serial micro dilution technique at concentration ranging from 5.00 mg/ml to 0.01 mg/ml. The minimum bactericidal concentration (MBC) was determined by sub culturing the microtitre wells showing no turbidity on the agar plate to obtain the MBC value. Both extracts showed substantial inhibitory effects against methicillin resistant coagulase negative Staphylococcus (MRCoNS) and methicillin resistant Staphylococcus aureus (MRSA). A slightly reduced inhibitory zone diameter was observed with MDR Acinetobacter sp. while no inhibitory effect was displayed among the extended spectrum beta lactamases (ESBL) K. pneumoniae and ESBL E. coli isolates. A significant difference in the zone sizes between both extracts was only observed in MRSA (p < 0.05). The MIC values ranged from 0.08 mg/ml to 0.63 mg/ml for aqueous and ethanol extracts against MRSA, MRCoNS and MDR Acinetobacter sp. while their MBC to MIC ratio values were 2 and less. The Q. infectoria gall extracts have shown very promising in vitro antibacterial activities and may be considered as a potentially good source of antimicrobial agent especially against MDR Gram positive bacteria.
    Matched MeSH terms: Complex Mixtures/isolation & purification; Complex Mixtures/pharmacology
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