The first study of charm quark diffusion with respect to the jet axis in heavy ion collisions is presented. The measurement is performed using jets with p_{T}^{jet}>60 GeV/c and D^{0} mesons with p_{T}^{D}>4 GeV/c in lead-lead (Pb-Pb) and proton-proton (pp) collisions at a nucleon-nucleon center-of-mass energy of sqrt[s_{NN}]=5.02 TeV, recorded by the CMS detector at the LHC. The radial distribution of D^{0} mesons with respect to the jet axis is sensitive to the production mechanisms of the meson, as well as to the energy loss and diffusion processes undergone by its parent parton inside the strongly interacting medium produced in Pb-Pb collisions. When compared to Monte Carlo event generators, the radial distribution in pp collisions is found to be well described by pythia, while the slope of the distribution predicted by sherpa is steeper than that of the data. In Pb-Pb collisions, compared to the pp results, the D^{0} meson distribution for 4
The first observation of the tt[over ¯]H process in a single Higgs boson decay channel with the full reconstruction of the final state (H→γγ) is presented, with a significance of 6.6 standard deviations (σ). The CP structure of Higgs boson couplings to fermions is measured, resulting in an exclusion of the pure CP-odd structure of the top Yukawa coupling at 3.2σ. The measurements are based on a sample of proton-proton collisions at a center-of-mass energy sqrt[s]=13 TeV collected by the CMS detector at the LHC, corresponding to an integrated luminosity of 137 fb^{-1}. The cross section times branching fraction of the tt[over ¯]H process is measured to be σ_{tt[over ¯]H}B_{γγ}=1.56_{-0.32}^{+0.34} fb, which is compatible with the standard model prediction of 1.13_{-0.11}^{+0.08} fb. The fractional contribution of the CP-odd component is measured to be f_{CP}^{Htt}=0.00±0.33.
In the planned research work, the nucleophilic substitution reaction of 1-[(E)-3-phenyl-2-propenyl]piperazine (1) was carried out with different sulfonyl chlorides (2a-g) at pH 9-10 to synthesize its different N-sulfonated derivatives (3a-g). The structures of the synthesized compounds were characterized by their proton-nuclear magnetic resonance (1H-NMR), carbon-nuclear magnetic resonance (13C-NMR) and Infra Red (IR) spectral data, along with CHN analysis. The inhibition potential of the synthesized molecules was ascertained against two bacterial pathogenic strains i.e. Bacillus subtilis and Escherichia coli. It was inferred from the results that some of the compounds were very suitable inhibitors of these bacterial strains. Moreover, their cytotoxicity was also profiled and it was outcome that most of these molecules possessed moderate cytotoxicity.
The polarizations of promptly produced χ_{c1} and χ_{c2} mesons are studied using data collected by the CMS experiment at the LHC, in proton-proton collisions at sqrt[s]=8 TeV. The χ_{c} states are reconstructed via their radiative decays χ_{c}→J/ψγ, with the photons being measured through conversions to e^{+}e^{-}, which allows the two states to be well resolved. The polarizations are measured in the helicity frame, through the analysis of the χ_{c2} to χ_{c1} yield ratio as a function of the polar or azimuthal angle of the positive muon emitted in the J/ψ→μ^{+}μ^{-} decay, in three bins of J/ψ transverse momentum. While no differences are seen between the two states in terms of azimuthal decay angle distributions, they are observed to have significantly different polar anisotropies. The measurement favors a scenario where at least one of the two states is strongly polarized along the helicity quantization axis, in agreement with nonrelativistic quantum chromodynamics predictions. This is the first measurement of significantly polarized quarkonia produced at high transverse momentum.
A search is presented for a narrow resonance decaying to a pair of oppositely charged muons using sqrt[s]=13 TeV proton-proton collision data recorded at the LHC. In the 45-75 and 110-200 GeV resonance mass ranges, the search is based on conventional triggering and event reconstruction techniques. In the 11.5-45 GeV mass range, the search uses data collected with dimuon triggers with low transverse momentum thresholds, recorded at high rate by storing a reduced amount of trigger-level information. The data correspond to integrated luminosities of 137 and 96.6 fb^{-1} for conventional and high-rate triggering, respectively. No significant resonant peaks are observed in the probed mass ranges. The search sets the most stringent constraints to date on a dark photon in the ∼30-75 and 110-200 GeV mass ranges.
Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide. Because of its high recurrence rate and heterogeneity, effective treatment for advanced stage of HCC is currently lacking. There are accumulating evidences showing the therapeutic potential of pharmacologic vitamin C (VC) on HCC. However, the metabolic basis underlying the anticancer property of VC remains to be elucidated. In this study, we used a high-resolution proton nuclear magnetic resonance-based metabolomics technique to assess the global metabolic changes in HCC cells following VC treatment. In addition, the HCC cells were also treated with oxaliplatin (OXA) to explore the potential synergistic effect induced by the combined VC and OXA treatment. The current metabolomics data suggested different mechanisms of OXA and VC in modulating cell growth and metabolism. In general, VC treatment led to inhibition of energy metabolism via NAD+ depletion and amino acid deprivation. On the other hand, OXA caused significant perturbation in phospholipid biosynthesis and phosphatidylcholine biosynthesis pathways. The current results highlighted glutathione metabolism, and pathways related to succinate and choline may play central roles in conferring the combined effect between OXA and VC. Taken together, this study provided metabolic evidence of VC and OXA in treating HCC and may contribute toward the potential application of combined VC and OXA as complementary HCC therapies.
A new 2-arylbenzofuran, sesbagrandiflorain C (1), together with four known compounds, 2-(3,4-dihydroxy-2-methoxyphenyl)-4-hydroxy-6-methoxybenzofuran-3-carbaldehyde (2), 2-(4-hydroxy-2-methoxyphenyl)-5,6-dimethoxybenzofuran-3-carboxaldehyde (3), sesbagrandiflorain A (4) and sesbagrandiflorain B (5), have been isolated from the stem bark of an Indonesian plant, Sesbania grandiflora (L.) Pers. The chemical structure of compound 1 was elucidated by UV, IR, MS, and NMR spectroscopic techniques. The proton and carbon NMR resonances of 1 were also compared with the predicted chemical shifts obtained from DFT quantum mechanical calculations with Gaussian. None of the compounds showed antibacterial activity against Bacillus subtilis, Escherichia coli, Mycobacterium smegmatis, Pseudomonas aeruginosa, and Staphylococcus aureus in an agar diffusion assay. However, sesbagrandiflorains A (4) and B (5) exhibited moderate activity against Mycobacterium tuberculosis H37Rv. In addition, compounds 1 - 5 have moderate cytotoxicity against HeLa, HepG2, and MCF-7 cancer cell lines.
The first search for supersymmetry in events with an experimental signature of one soft, hadronically decaying τ lepton, one energetic jet from initial-state radiation, and large transverse momentum imbalance is presented. These event signatures are consistent with direct or indirect production of scalar τ leptons (τ[over ˜]) in supersymmetric models that exhibit coannihilation between the τ[over ˜] and the lightest neutralino (χ[over ˜]_{1}^{0}), and that could generate the observed relic density of dark matter. The data correspond to an integrated luminosity of 77.2 fb^{-1} of proton-proton collisions at sqrt[s]=13 TeV collected with the CMS detector at the LHC in 2016 and 2017. The results are interpreted in a supersymmetric scenario with a small mass difference (Δm) between the chargino (χ[over ˜]_{1}^{±}) or next-to-lightest neutralino (χ[over ˜]_{2}^{0}), and the χ[over ˜]_{1}^{0}. The mass of the τ[over ˜] is assumed to be the average of the χ[over ˜]_{1}^{±} and χ[over ˜]_{1}^{0} masses. The data are consistent with standard model background predictions. Upper limits at 95% confidence level are set on the sum of the χ[over ˜]_{1}^{±}, χ[over ˜]_{2}^{0}, and τ[over ˜] production cross sections for Δm(χ[over ˜]_{1}^{±},χ[over ˜]_{1}^{0})=50 GeV, resulting in a lower limit of 290 GeV on the mass of the χ[over ˜]_{1}^{±}, which is the most stringent to date and surpasses the bounds from the LEP experiments.
In the current research work, different N-(substituted-phenyl)-4-{(4-[(E)-3-phenyl-2-propenyl]-1-piperazinyl}butanamides have been synthesized according to the protocol described in scheme 1. The synthesis was initiated by reacting various substituted anilines (1a-e) with 4-chlorobutanoyl chloride (2) in aqueous basic medium to give various electrophiles, 4-chloro-N-(substituted-phenyl)butanamides (3a-e). These electrophiles were then coupled with 1-[(E)-3-phenyl-2-propenyl]piperazine (4) in polar aprotic medium to attain the targeted N-(substituted-phenyl)-4-{(4-[(E)-3-phenyl-2-propenyl]-1-piperazinyl}butanamides (5a-e). The structures of all derivatives were identified and characterized by proton-nuclear magnetic resonance (1H NMR), carbon-nuclear magnetic resonance (13C NMR) and Infra-Red (IR) spectral data along with CHN analysis. The in vitro inhibitory potential of these butanamides was evaluated against Mushroom tyrosinase, whereby all compounds were found to be biologically active. Among them, 5b exhibited highest inhibitory potential with IC50 value of 0.013 ± 0.001 µM. The same compound 5b was also assayed through in vivo approach, and it was explored that it significantly reduced the pigments in zebrafish. The in silico studies were also in agreement with aforesaid results. Moreover, these molecules were profiled for their cytotoxicity through hemolytic activity, and it was found that except 5e, all other compounds showed minimal toxicity. The compound 5a also exhibited comparable results. Hence, some of these compounds might be worthy candidates for the formulation and development of depigmentation drugs with minimum side effects.
Results are reported from a search for new particles that decay into a photon and two gluons, in events with jets. Novel jet substructure techniques are developed that allow photons to be identified in an environment densely populated with hadrons. The analyzed proton-proton collision data were collected by the CMS experiment at the LHC, in 2016 at sqrt[s]=13 TeV, and correspond to an integrated luminosity of 35.9 fb^{-1}. The spectra of total transverse hadronic energy of candidate events are examined for deviations from the standard model predictions. No statistically significant excess is observed over the expected background. The first cross section limits on new physics processes resulting in such events are set. The results are interpreted as upper limits on the rate of gluino pair production, utilizing a simplified stealth supersymmetry model. The excluded gluino masses extend up to 1.7 TeV, for a neutralino mass of 200 GeV and exceed previous mass constraints set by analyses targeting events with isolated photons.
A search for narrow low-mass resonances decaying to quark-antiquark pairs is presented. The search is based on proton-proton collision events collected at 13 TeV by the CMS detector at the CERN LHC. The data sample corresponds to an integrated luminosity of 35.9 fb^{-1}, recorded in 2016. The search considers the case where the resonance has high transverse momentum due to initial-state radiation of a hard photon. To study this process, the decay products of the resonance are reconstructed as a single large-radius jet with two-pronged substructure. The signal would be identified as a localized excess in the jet invariant mass spectrum. No evidence for such a resonance is observed in the mass range 10 to 125 GeV. Upper limits at the 95% confidence level are set on the coupling strength of resonances decaying to quark pairs. The results obtained with this photon trigger strategy provide the first direct constraints on quark-antiquark resonance masses below 50 GeV obtained at a hadron collider.
In this report, a facile solution casting technique was used to fabricate polymer blend electrolytes of chitosan (CS):poly (ethylene oxide) (PEO):NH4SCN with high electrochemical stability (2.43V). Fourier transform infrared (FTIR) spectroscopy was used to investigate the polymer electrolyte formation. For the electrochemical property analysis, cyclic voltammetry (CV), linear sweep voltammetry (LSV), and electrochemical impedance spectroscopy (EIS) techniques were carried out. Referring to the FTIR spectra, a complex formation between the added salt and CS:PEO was deduced by considering the decreasing and shifting of FTIR bands intensity in terms of functional groups. The CS:PEO:NH4SCN electrolyte was found to be electrochemically stable as the applied voltage linearly swept up to 2.43V. The cyclic voltammogram has presented a wide potential window without showing any sign of redox peaks on the electrode surface. The proved mechanisms of charge storage in these fabricated systems were found to be double layer charging. The EIS analysis showed the existence of bulk resistance, wherein the semicircle diameter decreased with increasing salt concentration. The calculated maximum DC conductivity value was observed to be 2.11 × 10-4 S/cm for CS:PEO incorporated with 40 wt% of NH4SCN salt. The charged species in CS:PEO:NH4SCN electrolytes were considered to be predominantly ionic in nature. This was verified from transference number analysis (TNM), in which ion and electron transference numbers were found to be tion = 0.954 and tel = 0.045, respectively. The results obtained for both ion transference number and DC conductivity implied the possibility of fabricating electrolytes for electrochemical double layer capacitor (EDLC) device application. The specific capacitance of the fabricated EDLC was obtained from the area under the curve of the CV plot.
Herbs that are usually recognized as medicinal plants are well known for their therapeutic effects and are traditionally used to treat numerous diseases, including aging. This study aimed to evaluate the metabolite variations among six selected herbs namely Curcurmalonga, Oenanthejavanica, Vitex negundo, Plucheaindica, Cosmoscaudatus and Persicariaminus using proton nuclear magnetic resonance (1H-NMR) coupled with multivariate data analysis (MVDA). The free radical scavenging activity of the extract was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) assay. The anti-aging property was characterized by anti-elastase and anti-collagenase inhibitory activities. The results revealed that P. minus showed the highest radical scavenging activities and anti-aging properties. The partial least squares (PLS) biplot indicated the presence of potent metabolites in P. minus such as quercetin, quercetin-3-O-rhamnoside (quercitrin), myricetin derivatives, catechin, isorhamnetin, astragalin and apigenin. It can be concluded that P. minus can be considered as a potential source for an anti-aging ingredient and also a good free radical eradicator. Therefore, P. minus could be used in future development in anti-aging researches and medicinal ingredient preparations.
The search for the mechanism of ribosomal peptide bond formation is still ongoing. Even though the actual mechanism of peptide bod formation is still unknown, the dominance of proton transfer in this reaction is known for certain. Therefore, it is vital to take the quantum mechanical effects on proton transfer reaction into consideration; the effects of which were neglected in all previous studies. In this study, we have taken such effects into consideration using a semi-classical approach to the overall reaction mechanism. The M06-2X density functional with the 6-31++G(d,p) basis set was used to calculate the energies of the critical points on the potential energy surface of the reaction mechanism, which are then used in transition state theory to calculate the classical reaction rate. The tunnelling contribution is then added to the classical part by calculating the transmission permeability and tunnelling constant of the reaction barrier, using the numerical integration over the Boltzmann distribution for the symmetrical Eckart potential. The results of this study, which accounts for quantum effects, indicates that the A2451 ribosomal residue induces proton tunnelling in a stepwise peptide bond formation.
One of the biggest challenges of using single-chamber microbial fuel cells (MFCs) that utilize proton-exchange membrane (PEM) air cathode for bioenergy recovery from recalcitrant organic compounds present in wastewater is mainly attributed to their high internal resistance in the anodic chamber of the single microbial fuel cell (MFC) configurations. The high internal resistance is due to the small surface area of the anode and cathode electrodes following membrane biofouling and pH splitting conditions as well as substrate and oxygen crossover through the membrane pores by diffusion. To address this issue, the fabrication of new PEM air-cathode single-chamber MFC configuration was investigated with inner channel flow open assembled with double PEM air cathodes (two oxygen reduction activity zones) coupled with spiral-anode MFC (2MA-CsS-AMFC). The effect of various proton-exchange membranes (PEMs), including Nafion 117 (N-117), Nafion 115 (N-115), and Nafion 212 (N-212) with respective thicknesses of 183, 127, and 50.08 μ, was separately incorporated into carbon cloth as PEM air-cathode electrode to evaluate their influences on the performance of the 2MA-CsS-AMFC configuration operated in fed-batch mode, while Azorubine dye was selected as the recalcitrant organic compound. The fed-batch test results showed that the 2MA-CsS-AMFC configuration with PEM N-115 operated at Azorubine dye concentration of 300 mg L-1 produced the highest power density of 1022.5 mW m-2 and open-circuit voltage (OCV) of 1.20 V coupled with enhanced dye removal (4.77 mg L h-1) compared to 2MA-CsS-AMFCs with PEMs N-117 and N-212 and those in previously published data. Interestingly, PEM 115 showed remarkable reduction in biofouling and pH splitting. Apart from that, mass transfer coefficient of PEM N-117 was the most permeable to oxygen (KO = 1.72 × 10-4 cm s-1) and PEM N-212 was the most permeable membrane to Azorubine (KA = 7.52 × 10-8 cm s-1), while PEM N-115 was the least permeable to both oxygen (KO = 1.54 × 10-4) and Azorubine (KA = 7.70 × 10-10). The results demonstrated that the 2MA-CsS-AMFC could be promising configuration for bioenergy recovery from wastewater treatment under various PEMs, while application of PEM N-115 produced the best performance compared to PEMs N-212 and N-117 and those in previous studies of membrane/membrane-less air-cathode single-chamber MFCs that consumed dye wastewater.
Bi-directional signaling involved in radiation-induced bystander effect (RIBE) between irradiated carcinoma cells and their surrounding non-irradiated normal cells is relevant to radiation cancer therapy. Using the SPICE-NIRS microbeam, we delivered 500 protons to A549-GFP lung carcinoma cells, stably expressing H2B-GFP, which were co-cultured with normal WI-38 cells. The level of γ-H2AX, a marker for DNA double-strand breaks (DSB), was subsequently measured up to 24-h post-irradiation in both targeted and bystander cells. As a result, inhibition of gap junction intercellular communication (GJIC) attenuated DSB repair in targeted A549-GFP cells, and suppressed RIBE in bystander WI-38 cells but not in distant A549-GFP cells. This suggests that GJIC plays a two-way role through propagating DNA damage effect between carcinoma to normal cells and reversing the bystander signaling, also called 'rescue effect' from bystander cells to irradiated cells, to enhance the DSB repair in targeted cells.
The title compound, 2C14H14N4O·H2O, comprises a neutral mol-ecule containing a central pyrazol-3-one ring flanked by an N-bound phenyl group and a C-bound 5-methyl-1H-pyrazol-3-yl group (at positions adjacent to the carbonyl substituent), its zwitterionic tautomer, whereby the N-bound proton of the central ring is now resident on the pendant ring, and a water mol-ecule of crystallization. Besides systematic variations in geometric parameters, the two independent organic mol-ecules have broadly similar conformations, as seen in the dihedral angle between the five-membered rings [9.72 (9)° for the neutral mol-ecule and 3.32 (9)° for the zwitterionic tautomer] and in the dihedral angles between the central and pendant five-membered rings [28.19 (8) and 20.96 (8)° (neutral mol-ecule); 11.33 (9) and 11.81 (9)°]. In the crystal, pyrazolyl-N-H⋯O(carbon-yl) and pyrazolium-N-H⋯N(pyrazol-yl) hydrogen bonds between the independent organic mol-ecules give rise to non-symmetric nine-membered {⋯HNNH⋯NC3O} and {⋯HNN⋯HNC3O} synthons, which differ in the positions of the N-bound H atoms. These aggregates are connected into a supra-molecular layer in the bc plane by water-O-H⋯N(pyrazolide), water-O-H⋯O(carbon-yl) and pyrazolyl-N-H⋯O(water) hydrogen bonding. The layers are linked into a three-dimensional architecture by methyl-C-H⋯π(phen-yl) inter-actions. The different inter-actions, in particular the weaker contacts, formed by the organic mol-ecules are clearly evident in the calculated Hirshfeld surfaces, and the calculated electrostatic potentials differentiate the tautomers.
For the first time, a search for the rare decay of the W boson to three charged pions has been performed. Proton-proton collision data recorded by the CMS experiment at a center-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 77.3 fb^{-1}, have been analyzed. No significant excess is observed above the background expectation. An upper limit of 1.01×10^{-6} is set at 95% confidence level on the branching fraction of the W boson to three charged pions. This provides a strong motivation for theoretical calculations of this branching fraction.
Signals consistent with the B_{c}^{+}(2S) and B_{c}^{*+}(2S) states are observed in proton-proton collisions at sqrt[s]=13 TeV, in an event sample corresponding to an integrated luminosity of 143 fb^{-1}, collected by the CMS experiment during the 2015-2018 LHC running periods. These excited b[over ¯]c states are observed in the B_{c}^{+}π^{+}π^{-} invariant mass spectrum, with the ground state B_{c}^{+} reconstructed through its decay to J/ψπ^{+}. The two states are reconstructed as two well-resolved peaks, separated in mass by 29.1±1.5(stat)±0.7(syst) MeV. The observation of two peaks, rather than one, is established with a significance exceeding five standard deviations. The mass of the B_{c}^{+}(2S) meson is measured to be 6871.0±1.2(stat)±0.8(syst)±0.8(B_{c}^{+}) MeV, where the last term corresponds to the uncertainty in the world-average B_{c}^{+} mass.
The observation of single top quark production in association with a Z boson and a quark (tZq) is reported. Events from proton-proton collisions at a center-of-mass energy of 13 TeV containing three charged leptons (either electrons or muons) and at least two jets are analyzed. The data were collected with the CMS detector in 2016 and 2017 and correspond to an integrated luminosity of 77.4fb^{-1}. The increased integrated luminosity, a multivariate lepton identification, and a redesigned analysis strategy improve significantly the sensitivity of the analysis compared to previous searches for tZq production. The tZq signal is observed with a significance well over 5 standard deviations. The measured tZq production cross section is σ(pp→tZq→tℓ^{+}ℓ^{-}q)=111±13(stat)_{-9}^{+11}(syst) fb, for dilepton invariant masses above 30 GeV, in agreement with the standard model expectation.