Displaying publications 481 - 500 of 759 in total

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  1. Beng TS, Xin CA, Ying YK, Khuen LP, Yee A, Zainuddin SI, et al.
    J Palliat Care, 2020 Aug 14.
    PMID: 32791956 DOI: 10.1177/0825859720948976
    BACKGROUND: Hope is a positive coping mechanism that is important at all stages of illness, more so for palliative care patients.

    PURPOSE: To explore the experiences of hope of palliative care patients.

    METHODS: A qualitative study was conducted at University of Malaya, Kuala Lumpur, Malaysia. Adult palliative care in-patients were recruited and interviewed with semi structured questions on hope. Transcripts from the interviews were thematically analyzed with qualitative data management software NVIVO.

    FINDINGS: 20 palliative care patients participated in the study. The themes generated from thematic analysis were (1) The notions of hope, (2) The sources and barriers of hope and (3) The contents of hope.

    CONCLUSION: Hope is an ever-present source of energy that gives people strength to carry on even in the most adverse situations. Understanding hope from the palliative care perspective may allow healthcare providers to develop strategies to better foster hope in the terminally ill.

    Matched MeSH terms: Software
  2. Sultan T, Cheah CW, Ibrahim NB, Asif MK, Vaithilingam RD
    J Dent, 2020 Oct;101:103455.
    PMID: 32828845 DOI: 10.1016/j.jdent.2020.103455
    OBJECTIVES: This clinical study assessed and compared the linear and volumetric changes of extraction sockets grafted with a combination of Platelet-Rich Fibrin (PRF) and Calcium Sulfate (CS) (PRF-CS), and extraction sockets grafted with a combination of PRF and xenograft (X) (PRF-X).

    METHODS: Five single maxillary premolar extraction sockets received PRF-CS grafts and five single maxillary premolar sockets received PRF-X grafts. Linear (horizontal and vertical) measurements were accomplished using Cone Beam Computed Tomography (CBCT) images and volumetric changes were assessed using MIMICS software. Soft tissue level changes were measured using Stonecast models. All measurements were recorded at baseline (before extraction) and at 5-months post-extraction.

    RESULTS: Significant reduction in vertical and horizontal dimensions were observed in both groups except for distal bone height (DBH = 0.44 ± 0.45 mm, p = 0.09) and palatal bone height (PBH = 0.39 ± 0.34 mm, p = 0.06) in PRF-X group. PRF-CS group demonstrated mean horizontal shrinkage of 1.27 ± 0.82 mm (p = 0.02), when compared with PRF-X group (1.40 ± 0.85 mm, p = 0.02). Vertical resorption for mesial bone height (MBH = 0.56 ± 0.25 mm, p = 0.008), buccal bone height (BBH = 1.62 ± 0.91 mm, p = 0.01) and palatal bone height (PBH = 1.39 ± 0.87 mm, p = 0.02) in PRF-CS group was more than resorption in PRF-X group (MBH = 0.28 ± 0.14 mm, p = 0.01, BBH = 0.63 ± 0.39 mm, p = 0.02 and PBH = 0.39 ± 0.34 mm, p = 0.06). Volumetric bone resorption was significant within both groups (PRF-CS = 168.33 ± 63.68 mm3, p = 0.004; PRF-X = 102.88 ± 32.93 mm3, p = 0.002), though not significant (p = 0.08) when compared between groups. In PRF-X group, the distal soft tissue level (DSH = 1.00 ± 0.50 mm, p = 0.03) demonstrated almost 2 times more reduction when compared with PRF-CS group (DSH = 1.00 ± 1.00 mm, 0.08). The reduction of the buccal soft tissue level was pronounced in PRF-CS group (BSH = 2.00 ± 2.00 mm, p = 0.06) when compared with PRF-X group (BSH = 1.00 ± 1.50 mm, p = 0.05).

    CONCLUSIONS: PRF-CS grafted sites showed no significant difference with PRF-X grafted sites in linear and volumetric dimensional changes and might show clinical benefits for socket augmentation. The study is officially registered with ClinicalTrials.gov Registration (NCT03851289).

    Matched MeSH terms: Software
  3. Azi Simon Onyema, Leslie Than Thian Lung, Suresh Kumar, Rukman Awang Hamat
    MyJurnal
    Introduction: Group A streptococcus (GAS) is responsible for high morbidity and mortality globally. Hence, the need to develop sensitive, reliable and cost- effective method of detection is crucial. In this study, we developed a visual detection method for the common virulence gene, streptococcal pyrogenic exotoxin B (speB) involved in invasive GAS diseases using loop-mediated isothermal amplification (LAMP) with fluorescent detection dye (calcein). Meth-ods: The LAMP reaction was optimized at 63°C for 35 minutes using five sets of primer designed with LAMP primer V5 software. When the dye was added prior to amplification, samples with speB DNA developed a characteristic green color after the reaction, but no color reactions were observed in samples with DNAs of non-GAS isolates. De-tection of speB by LAMP assay was done among 43 clinical isolates of blood, pus, wound, tissue and throat samples and ATCCs for controls. Our findings were further reconfirmed by subjecting the LAMP products to 0.5% gel electro-phoresis. Results: The detection limit of this LAMP assay for speB was 10-7 ng/μl of genomic DNA per reaction, which was 10,000-fold more sensitive than conventional PCR 10-3 ng/μl. All 100 % samples were positive for speB gene by LAMP, and 93% by conventional PCR method. Conclusion: LAMP assay could offer remarkably high sensitivity, specificity, repeatability, reliability, affordability, and visibility; it is appropriate for rapid detection of speB in Group A streptococci (GAS) as a point of care testing.
    Matched MeSH terms: Software
  4. Harnois M, Himdi M, Yong WY, Rahim SKA, Tekkouk K, Cheval N
    Sci Rep, 2020 Feb 03;10(1):1714.
    PMID: 32015444 DOI: 10.1038/s41598-020-58657-5
    Manufacturing an array of high-quality metallic pattern layers on a dielectric substrate remains a major challenge in the development of flexible and 3-D frequency selective surfaces (FSS). This paper proposes an improved fabrication solution for the 3-D FSS based on water transfer printing (WTP) technology. The main advantages of the proposed solution are its ability to transform complicated 2-D planar FSS patterns into 3-D structures while improving both manufacturing quality and production costs. WTP technology makes use of water surface tension to keep the thin metallic patterns of the proposed FSS floating flat with the absence of a solid planar substrate. This feature enables these metallic FSS patterns to be transferred onto 3-D structures through a dipping process. To test the effectiveness of the proposed technique, the FSS was designed using computer simulation software Microwave Studio to obtain the numerical performance of the FSS structure. The WTP technology was then used to fabricate the proposed FSS prototype before its performance was tested experimentally. The measurement results agreed well with the numerical results, indicating the proposed manufacturing solution would support the development of complicated 3-D electronics devices, such as conformal antenna arrays and metamaterials.
    Matched MeSH terms: Software
  5. Momtaz YA, Mollaei P, Taheri-Tanjani P
    Curr Aging Sci, 2021 Feb 08.
    PMID: 33563165 DOI: 10.2174/1874609814666210208180051
    INTRODUCTION: One of the consequences of aging is the prevalence of chronic and age-related diseases, such as dementia. Caring for patients with dementia has a negative impact on the caregiver's well-being. This study aimed to examine the impact of cyberspace-based education on the well-being of caregivers of demented elderly people.

    METHODS: This experimental study was done on a sample of 86 caregivers of elderly with dementia in 2018. The study sample was selected from memory clinic of Taleghani Hospital and randomly assigned into groups (intervention n = 43, control n = 43 groups). The well-being was measured using the World Health Organization - Five Well-Being Index (WHO-5), before and two months after the intervention. Cyberspace-based educational intervention was conducted for one month. The SPSS software version 23 was employed in data analysis.

    RESULTS: The mean age of the caregivers in the intervention and control groups were (M = 51.95, SD = 10.90) and (M = 51.36, SD = 15.12) respectively. No significant difference was found between two groups in terms of age, gender and level of education. The results of analysis showed that while the well-being of the intervention group was significantly increased (t (38) = -11.38, P<0.001) the well-being in the control group was significantly reduced ( t(36) =4.71 , P<0.001).

    CONCLUSION: The findings showed that cyberspace-based education can improve the well-being of caregivers of the elderly with dementia.

    Matched MeSH terms: Software
  6. Etajuri EA, Suliman E, Mahmood WAA, Ibrahim N, Buzayan M, Mohd NR
    Dent Med Probl, 2021 1 16;57(4):359-362.
    PMID: 33448161 DOI: 10.17219/dmp/123976
    BACKGROUND: There is very little literature available on the reliability of the rapid prototyping technology in the production of three-dimension (3D)-printed surgical guides for accurate implant placement.

    OBJECTIVES: The aim of the study was to evaluate the deviation of implant placement performed with a surgical guide fabricated by means of the rapid prototyping technique (the PolyJet™ technology).

    MATERIAL AND METHODS: Twenty sheep mandibles were used in the study. Pre-surgical cone-beam computed tomography (CBCT) scans were acquired for the mandibles by using the Kodak 9000 3D cone-beam system. Two implants with dimensions of 4 mm in diameter and 10 mm in length were virtually planned on the 3D models of each mandible by using the Mimics software, v. 16.0. Twenty surgical guides were designed and printed using the PolyJet technology. A total of 40 implants were placed using the surgical guides, 1 on each side of the mandible (2 implants per mandible). The post-surgical CBCT scans of the mandibles were performed and superimposed on the pre-surgical CBCT scans. The amount of deviation between the virtually planned placement and the actual implant placement was measured, and a descriptive analysis was done.

    RESULTS: The results showed that the mean deviation at the implant coronal position was 1.82 ±0.74 mm, the mean deviation at the implant apex was 1.54 ±0.88 mm, the mean depth deviation was 0.44 ±0.32 mm, and the mean angular deviation was 3.01 ±1.98°.

    CONCLUSIONS: The deviation of dental implant placement performed with a 3D-printed surgical guide (the PolyJet technology) is within the acceptable 2-millimeter limit reported in the literature.

    Matched MeSH terms: Software
  7. Nur Atikah Asman, Suriyea Tanbin, Fazia Adyani Ahmad Fuad
    MyJurnal
    Dengue disease, which is caused by dengue virus (DENV) has been a major worldwide concern, with increased number of cases each year. Currently, there are no specific medications to treat the disease. Hence, there is a dire need to develop novel drugs for disease treatment. Glycolysis is a metabolic pathway that serves as the main source of energy for DENV replication and targeting the pathway is one of the ideal approach to discover new anti-DENV drugs. This paper focuses on the inhibition of human hexokinase isoform 2 (HK2) enzyme, which is one of the important enzymes in glycolysis, in the quest to disrupt DENV replication. In order to search for potential inhibitors, two methods were conducted, which are ligand-based screening and structure-based screening approaches. Docking of Daidzin , which was derived from Kudzu, a Japanese plant, into the active site of HK2 has shown the nearest binding affinity score (-7.94 kcal/mol) to glucose‘s (GLC), which is -8.15 kcal/mol. Meanwhile, Ethyl (2R)-2-[[3-[2-[(4-methylbenzoyl) amino]ethyl]-[1,2,4]triazolo[4,3-b]pyridazin-6-yl]sulfanyl]butanoate (Ethyl 2(R)), a compound which is the analogue of ((22E, 24R)-6β-methoxyergosta-7, 9(11), 22-triene-3β,5α-diol) or compound 2 from Ganoderma sinense obtained from ligand-based screening was also docked into the binding site of HK2, showing a binding affinity score of -8.43 kcal/mol. Both docking was conducted by using AutoDock4 software at virtual screening phase. These compounds were further analysed in an inhibition assay to determine the effects of these potential naturally-derived inhibitors on the activity of HK2. The outcome from the inhibition studies showed that both compounds exhibited substantial inhibition on the activity of HK2 enzyme, where Daidzin, at 0.5 mM, resulted in HK2 remaining activity of 87.28%, while Ethyl (2R) resulted in 70.09% of HK2 remaining activity at 0.5 mM concentration. The results also indicate that as the concentration of these compounds increased, the percentage of remaining enzyme activity decreased. In conclusion, this study has served as a platform for the development of anti-dengue drugs based on naturally-derived compounds, which is anticipated to be a safer option for dengue treatment.
    Matched MeSH terms: Software
  8. Ibrahim M, Abdul Azziz SSS, Wong CF, Bakri YM, Abdullah F
    Curr Comput Aided Drug Des, 2020;16(6):698-706.
    PMID: 31648647 DOI: 10.2174/1573409915666191015112320
    BACKGROUND: Obesity is one serious health condition that contributes to various chronic diseases. The inhibition of pancreatic lipase is a promising treatment for obesity.

    OBJECTIVE: The present study was designed to investigate anti-porcine pancreatic lipase effect of isolated compounds from Aquilaria subintegra and its mechanism.

    METHODS: Compounds were isolated with serial column chromatography and their structure were identified using spectroscopic methods. Isolated compounds were tested for anti-lipase potential activity using colorimetric assay. The prediction of energy binding between isolated compounds and enzyme was described using YASARA software.

    RESULTS: Four compounds were successfully isolated from the bark of A. subintegra, namely, 5- hydroxy-7,4'-dimethoxyflavone, luteolin-7,3',4'-trimethyl ether, 5,3'-dihydroxy-7,4'-dimethoxyflavone and β-sitosterol. The results indicated that all compounds displayed promising pancreatic lipase inhibitory activity ranging between of 6% to 53% inhibition. Compound 5-hydroxy-7,4'- dimethoxyflavone was a competitive inhibitor and decreases the enzyme catalysis. Meanwhile, β- sitosterol was a non- competitive inhibitor since the latter was bind allosterically toward enzyme.

    CONCLUSION: This finding is significant for further investigation of bioactive compounds from A. subintegra on animal study.

    Matched MeSH terms: Software
  9. Azlan Jaafar, Wan Mohamad Nasir, Nazirah Ab Mumin, Nur Najwa Athirah Elias, Muhaimin Afnan Mohd Sabri
    MyJurnal
    One of the roles expected of dental personnel is to promote dental seeking behaviour to the public. It is to ensure that adults maintain an optimum number of teeth for overall health and quality of life by enabling them to eat, speak and socialise without pain, discomfort or embarrassment. The objective of this study was to determine the reasons for seeking dental care and the factors that influence this decision among adult patients. This was a retrospective study of clinical records among patients of 18 years old and above who attended Universiti Sains Islam Malaysia (USIM) Dental Polyclinic, an academic dental centre, for the first time from January 2015 to December 2016. Socio-demographic data on age, gender and occupation as well as the reason for the visit were retrieved from the clinical records. Analysis of data was conducted using SPSS software version 21.0 and the pvalue was set at p
    Matched MeSH terms: Software
  10. Muhammadazril Mohd Saad, Nur Amin Abd Rahman, Khairani Idah Mokhtar, Noraini Abu Bakar, Azrul Fazwan Kharuddin, Wan Rohani Wan Taib
    MyJurnal
    Polymorphism in PAX9 (rs8004560), a gene responsible for craniofacial and tooth development, is often associated with Class II/Div2 malocclusion. This study aimed to detect the presence of PAX9 SNP (rs8004560) and to determine its genotype and allele distribution in Class II skeletal base malocclusion, contributed by retrognathic mandible, in the local Malaysian population. The association of PAX9 SNP (rs8004560) with Class II skeletal base malocclusion was also determined. A case control study was performed on 30 samples; 15 from Class II skeletal base malocclusion, and 15 from Class I skeletal base subject as control. Cephalometric measurements were performed prior to saliva samples collection. Genomic DNA was extracted from unstimulated saliva of all subjects, and the DNA was amplified using specific primers for marker rs8004560, followed by genotyping by sequencing. SHEsis online software was used to analyse Hardy-Weinberg Equilibrium (HWE) for cases and controls. Allelic and genotypic frequencies were compared between cases and controls. Significant difference in allele frequency was observed within the group whereby G allele was over-represented in the analysed population (p0.05). Although no genetic association between PAX9 SNP (rs8004560) with Class II skeletal base malocclusion was observed, significant difference in allele frequency observed might provide some indication in the involvement of PAX9 polymorphism in Class II skeletal base malocclusion contributed by retrognathic mandible. Further research utilising larger sample size will be required in order to determine the role of PAX9 gene in the aetiology of Class II skeletal base malocclusion observed in the local Malaysian population.
    Matched MeSH terms: Software
  11. Abu HB, Ludin SBM, Sowtali SNB
    J Public Health Res, 2021 Apr 14;10(2).
    PMID: 33855404 DOI: 10.4081/jphr.2021.2206
    BACKGROUND: This preliminary finding from a qualitative study examined the process of self-efficacy for the development of physical activity during myocardial infarction (MI) after recovery. A combination of healthy behaviors, including physical activity is the secondary prevention recommended to reduce the risk of recurrent MI. This study aims to understand how self-efficacy for physical activity is developed in a patient after MI by examining their perceptions and personal adherence to physical activity.

    DESIGN AND METHODS: This was a qualitative study and data was collected through semi-structured in-depth recorded phone interviews with eight Malay male participants. They were screened using a questionnaire and participants that met the inclusion criteria were interviewed, and were admitted to National Heart Centre, Malaysia between January to June 2019 diagnosed with MI. The data collected were analysed using NVivo 12 software and thematic analysis was applied.

    RESULTS: Four preliminary themes emerged from the study: 1) beliefs in physical activity; 2) healthy lifestyle: new normal or same old habit; 3) factors determining participation in pa; and 4) physical activity adherence strategies.

    CONCLUSIONS: The results of the studies showed that participants understand the need to maintain physical activity, which helps to maintain a healthy life after MI and prevent recurrent infarction. Strategies for developing self-efficacy for physical activity were also discussed. The need to understand that maintaining physical activity as well as adopting a new normal of healthy habit after MI is crucial in order to maintain the health and prevent recurrence of MI.

    Matched MeSH terms: Software
  12. Syarifah SM, Mohd Kassim AS, Mohd Aripin A, Chan CM, Zainulabidin MH, Ishak N, et al.
    Data Brief, 2021 Jun;36:107030.
    PMID: 34026964 DOI: 10.1016/j.dib.2021.107030
    This article presents experimental data on oil palm biomass (oil palm leaves, oil palm trunk and empty fruit bunch) handsheet production characterization by biodelignification treatment using Bacillus cereus extracted from termite gut (Coptotermus curvignathus). It associates the lignocellulose chemical composition obtained via technical association pulp and paper industry TAPPI T 222 om-02 testing on lignin content reduction determination, holocellulose and hemicellulose content determination (Kurscher-Hoffner method). Several data obtained for handsheet characterization presents brightness, opacity, contrast ratio, din transparency, thickness, bursting and tearing indexes are collected. Handsheet surface morphology was also observed on ratio of gaps differences between fiber bonding conducted using scanning electron microscope (SEM) and ImageJ software. The raw data findings supplement chemical composition analysis for both untreated and treated substrates on handsheet quality performance check as presented in the research article "Bio-Mechanical Pulping of Bacteria Pre-Treatment on Oil Palm Biomass for Handsheet Production" [1]. For understanding correlations into the difference among lignocellulose content composition which affect the handsheet formation and mechanical strength refer to article from this research [1]. This dataset is made publicly available for optimizing alternative waste material reuse in the pulp and paper industrial section.
    Matched MeSH terms: Software
  13. Melinda, Mei Lin Lau, Edmund, Sim Ui Hang
    MyJurnal
    Association between the expression of ribosomal protein (RP) genes and cancer is widely known. More specifically, the extra-ribosomal functions of RPs have been linked to carcinogenesis. The ribosomal protein gene, eL14 has been reported to be associated with malignancy of the colorectum, albeit of mechanism yet unclear. Its expression in cells derived from different tissue origin of colorectal carcinoma (CRC) has never been explored. Therefore, this study aims to comparatively analyse the expression pattern of eL14 between two different CRC cell lines (DLD-1 and HCT116). It involved a conventional gene expression analysis, the Reverse-Transcriptase PCR (RT-PCR) assays. Products of RT-PCR assay were resolved via an agarose gel electrophoresis method, and band intensities of amplicons were documented and quantified using TotalLab Quant software. We observed differential expression patterns of eL14 between DLD-1 and HCT116 cells, but statistical analysis revealed insignificant differences. Therefore, the relevance of eL14 as a biomarker to distinguish between different colorectal cancer cells is suggestive but not conclusive.
    Matched MeSH terms: Software
  14. Yahya N, Akhtar MN, Nasir N, Shafie A, Jabeli MS, Koziol K
    J Nanosci Nanotechnol, 2012 Oct;12(10):8100-9.
    PMID: 23421185
    In seabed logging the magnitude of electromagnetic (EM) waves for the detection of a hydrocarbon reservoir in the marine environment is very important. Having a strong EM source for exploration target 4000 m below the sea floor is a very challenging task. A new carbon nanotubes (CNT) fibres/aluminium based EM transmitter is developed and NiZn ferrite as magnetic feeders was used in a scaled tank to evaluate the presence of oil. Resistive scaled tank experiments with a scale factor of 2000 were carried out. X-ray Diffraction (XRD), Raman Spectroscopy and Field Emission Scanning Electron Microscope (FESEM) were done to characterize the synthesized magnetic feeders. Single phase Ni0.76Mg0.04Zn0.2Fe2O4, obtained by the sol-gel method and sintered at 700 degrees C in air, has a [311] major peak. FESEM results show nanoparticles with average diameters of 17-45 nm. Samples which have a high Q-factor (approximately 50) was used as magnetic feeders for the EM transmitter. The magnitude of the EM waves of this new EM transmitter increases up to 400%. A curve fitting method using MATLAB software was done to evaluate the performance of the new EM transmitter. The correlation value with CNT fibres/aluminium-NiZnFe2O4 base transmitter shows a 152.5% increase of the magnetic field strength in the presence of oil. Modelling of the scale tank which replicates the marine environment was done using the Finite Element Method (FEM). In conclusion, FEM was able to delineate the presence of oil with greater magnitude of E-field (16.89%) and the B field (4.20%) due to the new EM transmitter.
    Matched MeSH terms: Software
  15. Muhammad Najmi Mohammad Fauzi, Aisyah Aqilah Abu Bakar, Liyana Amalina Adnan, Tg Ainul Farha Tg Abdul Rahman, A’wani Aziz Nurdalila
    MyJurnal
    Bioinformatics tool is a software program made to extract meaningful information from the mass of molecular biology or biological databases and carry out sequence or structural analysis. The method of determining the order of nucleotides within a deoxyribonucleic acid (DNA) molecule is known as DNA sequencing. This analysis is meant to be run to the commercialized or factorymade goat's milk (pasteurised) from various states in Malaysia to identify the milk's authenticity, either it is pure or mixed with other foreign substances from other animals. The main objective is to compare DNA sequences of commercialized and raw goat's milk (handmilking and non-pasteurised). To achieve this, we used ClustalX to align and compare the obtained DNA from both milk samples. The sequences will be aligned using ClustalX software. ClustalX is a provider of an automated system for performing multiple alignments of sequences and profiles and evaluating the outcomes. The usage of ClustalX is helpful as it is cost-effective, user-friendly, and showing a high accuracy of the analysis.

    Matched MeSH terms: Software
  16. Sellvam D, Lau NS, Arip YM
    Trop Life Sci Res, 2018 Mar;29(1):37-50.
    PMID: 29644014 DOI: 10.21315/tlsr2018.29.1.3
    Malaysia is one of the countries that are loaded with mega biodiversity which includes microbial communities. Phages constitute the major component in the microbial communities and yet the numbers of discovered phages are just a minute fraction of its population in the biosphere. Taking into account of a huge numbers of waiting to be discovered phages, a new bacteriophage designated as Escherichia phage YD-2008.s was successfully isolated using Escherichia coli ATCC 11303 as the host. Phage YD-2008.s poses icosahedral head measured at 57nm in diameter with a long non-contractile flexible tail measured at 107nm; proving the phage as one of the members of Siphoviridae family under the order of Caudovirales. Genomic sequence analyses revealed phage YD-2008.s genome as linear dsDNA of 44,613 base pairs with 54.6% G+C content. Sixty-two open reading frames (ORFs) were identified on phage YD-2008.s full genome, using bioinformatics annotation software; Rapid Annotation using Subsystem Technology (RAST). Among the ORFs, twenty-eight of them code for functional proteins. Thirty two are classified as hypothetical proteins and there are two unidentified proteins. Even though majority of the coded putative proteins have high amino acids similarities to phages from the genus Hk578likevirus of the Siphoviridae family, yet phage YD-2008.s stands with its' own distinctiveness. Therefore, this is another new finding to Siphoviridae family as well as to the growing list of viruses in International Committee on Taxonomy of Viruses (ICTV) database.
    Matched MeSH terms: Software
  17. Sellvam, Dharmela, Yahya Mat Arip, Nyok, Sean Lau
    Trop Life Sci Res, 2018;29(1):37-50.
    MyJurnal
    Malaysia is one of the countries that are loaded with mega biodiversity which
    includes microbial communities. Phages constitute the major component in the microbial
    communities and yet the numbers of discovered phages are just a minute fraction of
    its population in the biosphere. Taking into account of a huge numbers of waiting to be
    discovered phages, a new bacteriophage designated as Escherichia phage YD-2008.s
    was successfully isolated using Escherichia coli ATCC 11303 as the host. Phage YD-2008.s poses icosahedral head measured at 57nm in diameter with a long non-contractile
    flexible tail measured at 107nm; proving the phage as one of the members of Siphoviridae
    family under the order of Caudovirales. Genomic sequence analyses revealed phage
    YD-2008.s genome as linear dsDNA of 44,613 base pairs with 54.6% G+C content.
    Sixty-two open reading frames (ORFs) were identified on phage YD-2008.s full genome,
    using bioinformatics annotation software; Rapid Annotation using Subsystem Technology
    (RAST). Among the ORFs, twenty-eight of them code for functional proteins. Thirty two are
    classified as hypothetical proteins and there are two unidentified proteins. Even though
    majority of the coded putative proteins have high amino acids similarities to phages from the
    genus Hk578likevirus of the Siphoviridae family, yet phage YD-2008.s stands with its’ own
    distinctiveness. Therefore, this is another new finding to Siphoviridae family as well as to the
    growing list of viruses in International Committee on Taxonomy of Viruses (ICTV) database.
    Matched MeSH terms: Software
  18. Sanus,M,A,, Nordin,M,A,, Rusli,M,R,, Mohamed,Z,N,h,
    Compendium of Oral Science, 2020;7(1):13-19.
    MyJurnal
    Abstract
    Objectives: This study aimed to assess intra- and inter-examiner reliability of International Caries Detection
    and Assessment System (ICDAS) and modified epidemiology ICDAS (MOD) code by undergraduate dental
    students with different clinical experiences.
    Methods: A total of 150 dental undergraduate students with varying clinical experiences (0, 1 and 2 years of
    clinical experience) were recruited. Participants received training through a theoretical lecture on ICDAS criteria
    by an experienced National Benchmark Group (NBG) examiner and underwent e-learning program prior to
    ICDAS calibration. Visual examination on extracted permanent teeth (N= 45) with different location and stages
    of caries progression ranging from ICDAS scores 0 to 6, was performed using the ICDAS criteria. The
    assessments were repeated after one hour. The data were analysed to evaluate inter-examiner and
    intra-examiner reliability in the form of kappa scores using SPSS 23 Software.
    Results: Mean kappa values for intra- and inter-examiner reliability for ICDAS code, were between 0.41 to
    0.60, and 0.61 to 0.80 respectively. For MOD code, mean kappa values for intra- and inter-examiner reliability
    were between 0.61 to 0.80. Good intra-examiner agreement (>0.61) was observed in both ICDAS and MOD
    code for all groups.
    Conclusion: All students performed similar agreement, therefore, clinical experience within 2 years does not
    influence the performance of visual inspection in detecting caries using ICDAS. The results of the study shows
    that ICDAS and modified epidemiology ICDAS codes has good reproducibility and is feasible to be used as a
    tool in clinical practice as well as patient education.
    Matched MeSH terms: Software
  19. Altay V, Karahan F, Öztürk M, Hakeem KR, Ilhan E, Erayman M
    J Plant Res, 2016 Nov;129(6):1021-1032.
    PMID: 27655558
    This paper covers studies on the molecular and ecological aspects of G. glabra var. glandulifera, G. flavescens ssp. flavescens and G. echinata collected from Hatay (Turkey); with the aim to better understand their genetic variation and ecological requirements for possible conservation programs. The material including total genomic DNA was extracted by the CTAB, and for PCR reaction, a total of 14 SSR primers developed for Medicago truncatula were used. PCR amplifications were performed in a Multigen(®) Thermal Cycler. Soil samples were analysed for their texture, pH, total soluble salts, calcium carbonate, total N content, total phosphorus and organic matter content. In order to see the association between genetic, ecological and geographical data, a similarity matrix was generated. Genetic similarity distances between genotypes were correlated with those of Eucledian distances obtained from ecological and geographical data. Analysis of molecular variance (AMOVA) was performed using GenAlEx 6.5 software to determine variation among and within genetic variations. The genetic analysis showed that the highest expected heterozygosity values were obtained from G. glabra while the lowest were obtained from G. echinata. In general heterozygosity values were low, especially for G. echinata. Therefore, variation appears to be lower within each species than among three species. The physical and chemical analysis of soil and plant samples indicates that mineral accumulation in plants is substantially affected by the soil characteristics. There is a need for identification of better strategies for the improvement of varieties, especially for small farmers managing marginal soils. More studies should be conducted in order to safeguard these taxa, especially G. glabra var. glandulifera which is collected intensively due to its economic value, the same is true for endemic taxon G. flavescens ssp. flavescens.
    Matched MeSH terms: Software
  20. Patrick, G. B., Nishibuchi, M., Tunung, R., Son, R.
    MyJurnal
    A total of 32 clinical strains of Vibrio cholerae, including members of the 01 and 0139 serogroup
    were collected from Klang, Selangor; Penang Island; Samarahan, Sarawak and Miri, Sarawak in Malaysia. In general, all the isolates except the 0139 serotype expressed low resistance to all the antibiotics tested with their Multiple Antibiotic Resistance (MAR) indices ranged from 0.10 to 0.48. The presence of ctx gene that encoded the cholera toxin was confirmed in all these clinical isolates by polymerase chain reaction. The results from the RAPD-PCR were analyzed using the RAPDistance software (Version 1.04). From the dendrogram generated, two main groups were observed which were subdivided into two clusters each. The Selangor’s isolates and the 0139 Penang’s isolates formed one group whereas the Samarahan, Sarawak isolates and the Miri, Sarawak isolates made up the other group, thus delineating their different sources of origin based on their geographical location.
    Matched MeSH terms: Software
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