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  1. Shahrin AA, Ghani SHA, Norman NH
    Korean J Orthod, 2021 Mar 25;51(2):86-94.
    PMID: 33678624 DOI: 10.4041/kjod.2021.51.2.86
    Objective: This study aimed to investigate the effect of micro-osteoperforations (MOPs) on external apical root resorption (EARR) during the initial orthodontic alignment phase of maxillary anterior crowding.

    Methods: Thirty patients (25 females, 5 males; mean age, 22.66 ± 3.27 years) who presented with moderate crowding of the upper labial segment and underwent extraction-based fixed appliance treatment were recruited. They were randomly allocated to receive adjunctive therapy with MOPs (n = 15) or treatment with fixed appliances only (control group; n = 15). EARR was measured from long-cone periapical radiographs taken at the start and the sixth month of treatment. A correction factor for the enlargement difference was used to calculate EARR. Data were analyzed with descriptive statistics and repeated-measures analysis of variance.

    Results: The mean root lengths of 168 teeth were measured and showed no statistically significant difference (p > 0.05) after six months of fixed appliance treatment in the MOP (mean difference [MD] = 0.13 mm; 95% confidence interval [CI] = -0.10-0.35) and control group (MD = 0.14 mm; 95% CI = -0.10-0.37). Most of the roots in the MOP and control groups (42.86% and 52.38%, respectively) showed only mild resorption. Less than 8% of the roots in both groups (7.14% in the MOP group and 4.76% in the control group) showed moderate resorption.

    Conclusions: Acceleration of orthodontic tooth movement with adjunctive MOPs therapy during the alignment phase does not exacerbate EARR in patients with moderate crowding of the upper labial segment in comparison with controls.

    Matched MeSH terms: Female
  2. Tan LT, Nathan AM, Jayanath S, Eg KP, Thavagnanam S, Lum LCS, et al.
    Pediatr Pulmonol, 2020 12;55(12):3477-3486.
    PMID: 33002341 DOI: 10.1002/ppul.25083
    BACKGROUND: Provision of home mechanical ventilation (HMV) to children with chronic respiratory insufficiency enhances growth and quality of life. The hypothesis was that health-related quality of life (HRQoL) and the development of these children were poorer than in healthy children.

    OBJECTIVES: To determine the HRQoL and developmental outcome of children on HMV.

    METHODS: This cross-sectional study used the TNO-AZL Preschool children's Quality Of Life (TAPQOL; <5 years old) and Health Utilities Index (HUI) 2/3 (≥5 years old) to assess the quality of life and the Schedule of Growing Skills-II to assess development. Instruments were used on children currently or previously on HMV (≥3 months) and compared with age and sex-matched controls.

    RESULTS: Sixty-five patients and 130 controls were recruited. Patients' median (interquartile range) age was 3.12 (1.65, 5.81) years. Patients had significantly lower TAPQOL scores in the domains of lung, liveliness, positive mood, social functioning, motor functioning, and communication, and lower HUI 2/3 scores in hearing, sensation, pain, speech, mobility, ambulatory, dexterity, and self-care domains. The developmental outcome of patients was poorer in all domains. However, patients had fewer behavioral problems. Those with respiratory tract disease and without comorbidities had better HRQoL and developmental scores. Having a parent as the primary caregiver was associated with better speech and language skills.

    CONCLUSIONS: HRQoL and the developmental outcome are lower in children on HMV compared to controls. Children with respiratory tract disease and without comorbidities have a better outcome. Parents play a crucial role in the acquisition of speech.

    Matched MeSH terms: Female
  3. Gan F, Vikneswaran V, Yu KK
    Med J Malaysia, 2021 03;76(2):273-274.
    PMID: 33742646
    A 32-year-old, gravida 2 para 0+1, was managed in Selayang Hospital, Selangor for uterine fibroids in pregnancy and placenta previa major. The lady went into preterm labour at 33 weeks, requiring emergency Caesarean section. Intraoperatively, we found a thinned-out bulge between the intramural uterine fibroids at the posterior uterine wall, which then perforated and was repaired. Persistent bleeding post operatively led to relaparotomy and hysterectomy. Histology of the uterus reported arteriovenous malformation (AVM). We postulate the possibility of these lesions coexisting with uterine fibroids. Screening for uterine AVMs in patients with fibroids may lead to early detection with option of embolization; deferring the need for hysterectomy.
    Matched MeSH terms: Female
  4. Ehab Helmy Abdel Malek Fahmy, Constance Liew Sat Lin, Alvin Oliver Payus, Rhanye Mac Guad, May Zaw Soe, Satishratnam Nagaratnam, et al.
    MyJurnal
    Dysmenorrhea is one of the leading causes of pelvic pain and menstrual disorder among women during childbearing age. The burden of dysmenorrhea is greater than any other gynaecological complaint. Some women have severe dysmenorrhea which renders them incapacitated for days each menstrual cycle requiring absence from study or duty, frequently requiring pain killer, restriction of daily performance, poor sleep, negative moods such as anxiety and depression. A 31-year-old female presented with severe dysmenorrhea and heavy menstrual bleeding (HMB) as a cause of multiple uterine fibroids, underwent surgeries to remove 100 fibroids from her uterus which has improved her quality of life, eliminating her dysmenorrhea and menstrual abnormalities
    Matched MeSH terms: Female
  5. Ng TK
    Asian J Psychiatr, 2010 Jun;3(2):76-7.
    PMID: 23051195 DOI: 10.1016/j.ajp.2010.03.008
    Study period: 1 February 2008 till 1 August 2008
    Study participants: 110 type 2 diabetes patients, 40% male, 96% malay
    Study design: Cross-sectional study
    Questionnaire: Beck depression inventory II (BDI-II) in Malay version
    Prevalence of depression: 22%
    Study site: Outpatient clinic, Hospital Sik, Kedah, Malaysia
    Matched MeSH terms: Female
  6. Dahlan SA, Mohamed M
    MyJurnal
    ABSTRACTS FOR INTERNATIONAL FAMILY HEALTH CONFERENCE 2019 (I-FaH 2019). Health Intervention Towards Community Wellness, Held at Oriental Crystal Hotel, Kajang, Selangor, Malaysia. 4-5th September, 2019
    Introduction: In Malaysia, the proportion of safe deliveries remains above 98% since 2010. Safe delivery is defined as deliveries conducted by trained personnel; either doctors, nurses with midwifery training or community nurses. Despite the good coverage of trained personnel and continuous effort to promote birth preparedness among antenatal mothers, unsafe delivery still occur in Malaysia and reported as 0.5% in NHMS 2016.
    Methods: A retrospective analysis on the characteristics of unsafe deliveries from the national surveillance data for the period of 2015 to 2017 was carried out. Characteristics of mothers are extracted from the reports of unsafe delivery investigation by Ministry of Health Malaysia and descriptive analyses are carried out by year for trending.
    Results: Total of 985 unsafe deliver-ies were reported and investigated in 2015, 1201 in 2016 and 1045 in 2017. About 37.7% to 49.9% (n=453-521) of these deliveries were among Malaysians followed by 22.9% to 40% (n=239-394) among non-citizen without identification document and 11.1% to 27.6% (109-332 deliveries) among non-citizen with identification document. It is commonly reported among mothers aged 26 to 35 years old (38.6% to 43.4%; n=454-463) and multiparous (47.3% to 52.0%; n=494-512). About 25.5% to 35.4% (n=251-370) of these mothers never attended antenatal check-up. Five main reported reasons which had contributed to the unsafe deliveries in 2015 to 2017 were; invalid identification documents, financial constraints, transportation problems, far distance from health facilities and mother’s personal choice.
    Conclusion: These findings translate into possible intervention targeted the above population on unsafe deliveries. Multi-sectoral strategies and inter-agency partnership are needed in strengthening the intervention.
    Matched MeSH terms: Female
  7. Ariaratnam S, Rodzlan Hasani WS, Krishnapillai AD, Abd Hamid HA, Jane Ling MY, Ho BK, et al.
    PLoS One, 2020;15(9):e0238566.
    PMID: 32915860 DOI: 10.1371/journal.pone.0238566
    BACKGROUND: Obesity is a crucial public health problem and is rising globally. This study was conducted to determine the prevalence of obesity and its associated factors among the elderly in Malaysia.
    METHODS: Data were obtained from the National Health and Morbidity Survey (NHMS) 2015. This was a cross sectional population-based study using a two stage stratified random sampling design. Elderly population aged 60 years and above was selected. Data were collected using pre-tested self-administered questionnaire in the form of sociodemographic profile, non-communicable diseases (NCD) comorbidities (status on hypertension, diabetes and hypercholesterolemia) and NCD risk factors (current smoker and physical activity). Obesity has been defined using the World Health Organization (WHO) Body Mass Index (BMI) guideline, 1998. A complex sampling design analysis was used for the descriptive statistics. The associated risk factors for obesity were identified using Multiple Logistic Regression analysis.
    RESULTS: A total of 3794 respondents were included in this study. The prevalence of obesity among Malaysian elderly was 30.2%. The prevalence of obesity among the elderly was significantly higher in females, respondents from urban areas and Indians. Approximately one third of the elderly with non-communicable diseases such as hypertension (33.1%) and diabetes (38.8%), respectively, were obese. Among elderly who were obese, majority of them (89.8%) had at least one NCD. The prevalence of obesity was 16.8% among current smokers (aOR 0.59). Multiple logistic regression analysis revealed that elderly females (aOR [adjusted odds ratio] 1.52), having secondary education (aOR 1.96) with household income of RM 3000 and above (aOR 1.57) as well as being hypertensive (aOR 1.61) and diabetic (aOR 1.50) were more likely to be obese. In contrast, the Chinese elderly respondents (aOR 0.62) and current smokers (aOR 0.59) were less likely to be obese. There were no significant associations of obesity with hypercholesterolemia or with physical activity.
    CONCLUSIONS: A substantial proportion of Malaysian elderly were obese, and factors associated with obesity among them were being female, having secondary education with a household income of RM 3000 and above and being hypertensive or diabetic. Enhanced health promotion and education should be targeted at younger people in order to prevent obesity in the later years.
    Study name: National Health and Morbidity Survey (NHMS-2015)
    Matched MeSH terms: Female
  8. Leong Joyce W.S, Intan Nadia Mohd Zukri, Siew Mooi Ching, Navin Kumar Devaraj
    MyJurnal
    Introduction: Falls among the elderly can be associated with serious complications such as fractures, injuries and death. This study aims to ascertain the factors associated with falls among the elderly patients attending a government clinic located in Kuala Lumpur. Method: This was a cross-sectional study using a convenience sampling method. Data collection in 2017 from 322 elderly who attended the above clinic. A modified assisted self-administered ques- tionnaire was used that contained the socio-demographic data, falls profile as well as extrinsic and intrinsic factors associated with falls. Analysis was done with SPSS v20.0. Results: 120 (37.27%) elderly reported falls in the past one year. The majority who had falls were females (n=76, 41.8%) and between the age of 80-89 years old (n=29, 44.6%). Malay ethnicity group, reported more falls compared to other ethnicities (n=93,44.5%). Significant associations were found between age, ethnicity and history of falls with falling (p
    Matched MeSH terms: Female
  9. Mohamed Faizal Sikkandar, Shalimar Abdullah, Rajesh Singh, Parminder Singh Gill, Nur Azuatul Akmal Kamaludin, Tan Jin Aun, et al.
    MyJurnal
    Introduction: Compression of the median nerve in pregnancy is thought to be due to fluid retention within the carpal tunnel space. We aim to discover the cause of carpal tunnel syndrome (CTS) in pregnancy using high resonance ul- trasonography. Methods: This is a cross-sectional study where obstetric patients were screened for CTS and subjected to a non invasive ultrasonic imaging. Results: A total of 63 patients were seen with 25 diagnosed to have CTS (39.7%) and 38 patients had none (60.3%) based on a screening tool. Age ranged from 20-42 years old with the highest range in the 28-30 year old group (34.9%). In patients with CTS, the cross sectional area of the median nerve inside the tunnel was a mean of 0.908 cm ie larger, while non-CTS patients had a mean of 0.797 cm inside the tunnel. The transverse carpal ligament (TCL) measured a mean of 0.0988 cm in the CTS group (ie thinner) and 0.1058 cm in the non-CTS group. Median nerve mobility at equal to or less than one tendon width was 80% in pregnant women with CTS and 92.1% for those without. No fluid was present within the carpal tunnel of all patients. The results were sta- tistically not significant. Conclusion: Ultrasonographic evidence in pregnant women with CTS shows a larger median nerve, a more mobile median nerve and a less thick transverse carpal ligament. There is absence of fluid retention and synovitis ruling out extrinsic compression of the median nerve as cause of CTS in pregnancy.
    Matched MeSH terms: Female
  10. Siti Norashikin Mohd Tambeh, Sumitabha Ghosh, Mohd Hamim Rajikin
    MyJurnal
    Introduction: The present study aims to investigate the effects of nicotine on foetal loss,
    postnatal growth and corresponding levels of oestrogen and progesterone in pregnant rats.
    Methods: Subcutaneous injection of nicotine tartrate (7.5 mg/kg/day) was administered to
    groups of pregnant rats; with treatment scheduled from day 1 through day 5, day 5 through
    day 9 or day 1 through day 9 of pregnancy. On day 10 of pregnancy, laparotomy was
    performed to count the number of blastocyst implantation sites. During parturition, the
    number of viable pups was recounted and statistically compared with the controls. One
    group of rats which received nicotine from day 1 through day 9 of pregnancy was sacrificed
    on day 16 of pregnancy, and circulating levels of oestrogen and progesterone were
    measured. Upon delivery, the birth weight of the pups was measured, and their weights were
    recorded until weaning. Results: There was a significant increase in foetal loss particularly in
    rats which received nicotine from day 5 through day 9 and from day 1 through day 9 of
    pregnancy. There was also significantly lower birth weight of pups in all groups; however,
    this pattern did not continue until weaning. Plasma oestrogen level was significantly elevated
    with a significant decrease in the plasma progesterone level. Conclusions: Nicotine
    administration during pregnancy showed an increase in foetal loss with a corresponding
    increase in oestrogen and decrease in progesterone levels. Although the birth weight of the
    pups was low, there was catch-up growth in the pups.
    Matched MeSH terms: Female
  11. Noor Azura Noor Mohamad, Suzanna Daud, Sun Ta Thow, Darminder Singh Chopra, Bahiyah Abdullah
    MyJurnal
    Heterotopic interstitial pregnancy in natural conception is very rare. Definitive diagnosis is
    made by systematic pelvic ultrasound. Simultaneous viable conception observed in both intraand extra-uterine is pathognomonic of heterotopic interstitial pregnancy. We report a 34-yearold woman primigravida at 10 weeks’ amenorrhoea who underwent evacuation of retained
    product of conception for missed miscarriage. Intraoperatively, minimal product of conception
    was retrieved. Bedside pelvic ultrasound showed cystic mass at right superolateral part of
    uterine fundus suspicious of interstitial pregnancy. Cornual resection was performed via
    laparotomy. Histopathological examination showed presence of product of conception and
    interstitial pregnancy. Serum β-human chorionic gonadotropin dropped from 10,027 IU/l on day
    one post-ERPC to 210 IU/l at day three post-cornual resection. This case report highlighted
    the challenge in diagnosing heterotopic interstitial pregnancy conceived naturally. Timely
    diagnosis may avert dire consequences of massive haemorrhage from ruptured uterine cornua
    Matched MeSH terms: Female
  12. Liyana Dhamirah Aminuddin, Sabrina Ab Wahab, Suhaili Shariffudin, Tarita Tai
    MyJurnal
    Patients with lung cancer may present with respiratory and systemic symptoms. However,
    cutaneous metastases from primary lung cancer is a rare phenomenon, especially in women,
    that signifies a poor prognosis. This paper reported a case regarding a 71-year-old woman
    who was first presented with a cutaneous nodule over the year. Her condition was further
    progressed to multiple lesions on the back and abdomen, dyspnoea, haemoptysis and weight
    loss. The results of the skin lesion biopsy exhibited metastatic lung adenocarcinoma with
    positive immunohistochemistry for thyroid transcription factor 1 (TTF1) and cytokeratin 7 (CK7). Computed tomography (CT) scan was conducted, and it revealed a left upper lobe lung
    mass. The patient was subsequently scheduled for additional management, but she had
    succumbed to complications of pulmonary embolism before the necessary interventions could
    be provided. In this particular case presentation, the biopsy of cutaneous lesions obtained had
    revealed an undiagnosed primary malignancy.
    Matched MeSH terms: Female
  13. Sedigheh Moghadam, Hairul Anuar Hashim
    MyJurnal
    Introduction: Regular aerobic exercise and low-fat milk consumption can improve certain cognitive functions and reducing emotional distress. However, the impacts of combining these modalities are less explored. Thus, the present study examined the effects of combining aerobic exercise and low-fat milk intake among sedentary female students. Methods: Using a nonrandomized pretest – posttest with a control group research design, 85 sedentary females aged 16 years old were assigned into either milk (n = 21), exercise (n = 22), combined low fat milk and exercise (n = 23), or control (n = 19) groups. One serving of low-fat milk was provided to the students during each school day, and a 1-hour supervised step aerobics exercise was conducted twice per week for 3 months. Emotional distress and sus- tained attention were measured at baseline, 6th and 12th weeks after the intervention. A mixed factorial ANOVA was used to analyse the data. Results: The results revealed significantly less emotional distress in the combined (p < 0.01) and exercise groups (p < 0.05) compared with the control group after 12 weeks. Additionally, significant reductions were observed in the total time taken and errors of omission for both digits 6 and 9 of the Digit Vigilance Test in the combined group (p < 0.05, p < 0.001) compared with the control group. Conclusions: The results showed that low fat milk alone did not provide any additional benefits related to distress regulation, but the combination of exercise and low-fat milk contributed to improving sustained attention.
    Matched MeSH terms: Female
  14. Muhammad Wafiuddin, Ahmad Faizal Roslan, Ahmad Hafiz Zulkifly
    MyJurnal
    Tuberculosis (TB) is an ancient disease dated back from ancient Greece time. Once rare in developed countries, now it has re-emerged due to immigration and secondary immunodeficiency. A 27-year-old lady had left knee pain for the past 4 years, went for knee diagnostic arthroscopy procedure, and diagnosed as pigmented villonodular synovitis (PVNS) of the left knee. Despite regular analgesics and physiotherapy, patient symptoms worsen. Two years after the initial treatment, the patient went for a further workout and diagnosed as knee tuberculosis, commenced on anti-TB treatment, the patient still left complicated with a stiff knee and fixed flexion deformity. Identification of knee tuberculosis during the initial phase is crucial as late diagnosis and treatment will leave the patient with debilitating complications.
    Matched MeSH terms: Female
  15. Ernieenor FCL, Apanaskevich DA, Ernna G, Mariana A
    Exp Appl Acarol, 2020 Jan;80(1):137-149.
    PMID: 31832837 DOI: 10.1007/s10493-019-00439-4
    Identifying certain species of Dermacentor ticks in Malaysia is challenging as there is no comprehensive work on their systematics and lack of specific taxonomic keys. In this study, we described and characterized D. steini ticks collected from a forest reserve in the vicinity of the Forest Research Institute of Malaysia using integrated phenotypic and genotypic traits. In total two males and three females of questing D. steini ticks were morphologically identified using specific illustrated taxonomic keys based on their special characters. Further confirmation and characterization of the tick species were then examined using PCR, followed by sequencing partial mitochondrial 16S rDNA gene (mt-rrs). Clustering analysis based on mt-rrs was carried out by constructing neighbor-joining tree topology to clarify the genetic variation of local D. steini. Based on external morphological characterizations, all ticks were successfully identified down to the species as adult D. steini. The molecular traits based on phylogenetic tree provide very strong support for the monophyletic clade of D. steini including high percentages of similarity (97-100%) with available sequences in GenBank. Furthermore, a low intraspecific variation (4%) among the species of D. steini was observed but it was genetically different from other Dermacentor species with high interspecific value (8-15%). These findings produced the first genotypic data of D. steini using 16S rDNA gene which confirmed the presence of this species in Malaysia. Moreover, this study supports the taxonomic status of local D. steini and adds to the knowledge of accurate identification of ticks.
    Matched MeSH terms: Female
  16. Rathika Rajah, Alvin Oliver Payus, Norlaila Mustafa
    MyJurnal
    Antiphospholipid syndrome is a hypercoagulable autoimmune condition that predominantly affect the female and commonly manifest as arterio-venous thrombosis and recurrent miscarriage. Here, we present a unique case of a healthy young man who developed sudden onset of right leg swelling after exercise which was then found to be due to deep vein thrombosis via Doppler ultrasound. His blood investigations showed thrombocytopenia and prolonged coagulation profile. Therefore, antiphospholipid syndrome was suspected and later confirmed by positive autoim- mune antibodies. He was treated with long term moderate intensity oral warfarin. The objective of this case report is to share the uncommon occurrence of an unprovoked deep vein thrombosis secondary to antiphospholipid syn- drome in a healthy young man so that the possibility of deep vein thrombosis should be suspected in selected cases of non-resolving leg swelling after exercise as it can lead to fatal pulmonary embolism.
    Matched MeSH terms: Female
  17. Syarifah Mohd Rahim, Siti Norbaya Masri, Rosni Ibrahim
    MyJurnal
    Automated microbial detection system (AMDs) are design to detect early growth of bacterial and fungal. We herein report a rare case of false positive blood culture by AMDs in Plasmodium falciparum infection. A 41-year-old pre- viously healthy lady, with recent history of travelling to Lagos, Nigeria had presented to the casualty with history of fever and lethargy for three days. There was no malaria prophylaxis taken prior to the travelling history. Peripheral blood smear confirmed the presence of young trophozoite of Plasmodium falciparum with parasitemia of 7%. Con- current blood culture sent was positive, however all subcultures were negative for any growth. She was treated with intravenous artesunate however succumbed to death on the day of admission due to severe falciparum infection complicated with multiorgan failure and shock. The aim of this report is to highlight, the circumstances that can trigger the false positive AMDs detection and the possible underlying mechanism.
    Matched MeSH terms: Female
  18. Tamin NSI, Razalli KA, Sallahuddin SN, Chan HK, Hassan MRA
    Cancer Epidemiol, 2020 12;69:101829.
    PMID: 32998070 DOI: 10.1016/j.canep.2020.101829
    INTRODUCTION: The immunochemical fecal occult blood test (iFOBT) has been widely used for opportunistic colorectal cancer (CRC) screening in average-risk individuals seeking care from public health clinics in Malaysia. This study provides a 5-year outcome evaluation of such a practice.

    METHODS: The findings for a few outcome indicators, ranging from the iFOBT uptake to the CRC and polyp detection rates, were generated from the data contributed by 583 public health clinics between 2014 and 2018. The trends in their changes were also evaluated.

    RESULTS: The iFOBT uptake constantly increased over the years (p < 0.001), totaling 2.29 % (n = 127,957) as at 2018. Nearly 10 % (n = 11,872) of the individuals screened had a positive test result. Of those who underwent colonoscopy (n = 6,491), 4.04 % (n = 262) and 13.93 % (n = 904) were found to have CRC and polyps, respectively.

    CONCLUSION: An uptrend in the CRC screening uptake was witnessed following the introduction of the iFOBT in public health clinics.

    Matched MeSH terms: Female
  19. Samson KLI, Loh SP, Khor GL, Mohd Shariff Z, Yelland LN, Leemaqz S, et al.
    BMJ Open, 2020 02 05;10(2):e034598.
    PMID: 32029499 DOI: 10.1136/bmjopen-2019-034598
    INTRODUCTION: Folic acid (0.4 mg) taken prior to and during early pregnancy reduces the risk of neural tube defects (NTDs). Because these birth defects occur early in pregnancy, before women may know they are pregnant, many countries have mandated the addition of folic acid to food staples. In countries where fortification is not possible, and weekly iron folic acid programmes exist to reduce anaemia, the WHO recommends that 2.8 mg (7×0.4 mg) folic acid be given instead of the current weekly practice of 0.4 mg. Currently, there is a lack of evidence to support if the 2.8 mg folic acid per week dose is sufficient to raise erythrocyte folate concentrations to a level associated with a reduced risk of a NTD-affected pregnancy. We aim to conduct a three-arm randomised controlled trial to determine the effect of weekly folic acid with iron on erythrocyte folate, a biomarker of NTD risk.

    METHODS AND ANALYSIS: We will recruit non-pregnant women (n=300; 18-45 years) from Selangor, Malaysia. Women will be randomised to receive either 2.8, 0.4 or 0.0 (placebo) mg folic acid with 60 mg iron weekly for 16 weeks, followed by a 4-week washout period. The primary outcome will be erythrocyte folate concentration at 16 weeks and the mean concentration will be compared between randomised treatment groups (intention-to-treat) using a linear regression model adjusting for the baseline measure.

    ETHICS AND DISSEMINATION: Ethical approval was obtained from the University of British Columbia (H18-00768) and Universiti Putra Malaysia (JKEUPM-2018-255). The results of this trial will be presented at scientific conferences and published in peer-reviewed journals.

    TRIAL REGISTRATION NUMBERS: ACTRN12619000818134 and NMRR-19-119-45736.

    Matched MeSH terms: Female
  20. Ibrahim MA, Zulkifli SZ, Azmai MNA, Mohamat-Yusuff F, Ismail A
    Animals (Basel), 2021 Mar 12;11(3).
    PMID: 33809309 DOI: 10.3390/ani11030798
    Compound 3,4-dichloroaniline (3,4-DCA) is a metabolite of several urea herbicides and intermediate chemical of several industrial products. Moreover, 3,4-DCA has been frequently detected in aquatic ecosystems around the world. This aniline is more toxic than the parent chemicals, and it affects non-target organisms. This study evaluated a 21-day reproductive response of an emerging aquatic vertebrate model, Javanese medaka (Oryzias javanicus), exposed to 3,4-DCA. Fecundity and gonads histopathology were observed. The spawning rate and fertilisation reduced significantly in the highest exposed-group (250 µg/L). Gonadosomatic index (GSI) was significantly low in females exposed to 250 µg/L. No substantial structural alteration of male gonads. However, oocyte development and ovarian cell structure were disrupted in 250 µg/L exposed females. The gonadal developmental was not affected in the males; however, a significant reduction in the developmental of female gonads was observed at 250 µg/L. These results show that 3,4-DCA interfere with the reproduction of Javanese medaka through fecundity and alteration of gonadal tissues.
    Matched MeSH terms: Female
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