Displaying publications 61 - 80 of 232 in total

Abstract:
Sort:
  1. Erythrocyte Binding Activity of PkDBPαII of Plasmodium knowlesi Isolated from High and Low Parasitemia Cases
    Mohd Bukhari FD, Lau YL, Fong MY
    Am J Trop Med Hyg, 2020 Dec 14.
    PMID: 33319732 DOI: 10.4269/ajtmh.20-0797
    Invasion of Plasmodium knowlesi merozoite into human erythrocytes involves molecular interaction between the parasite's Duffy binding protein (PkDBPαII) and the Duffy antigen receptor for chemokines on the erythrocytes. This study investigates the binding activity of human erythrocyte with PkDBPαII of P. knowlesi isolates from high and low parasitemic patients in an erythrocyte binding assay. The binding activity was determined by counting the number and measuring the size of rosettes formed in the assay. The protein PkDBPαII of P. knowlesi isolated from low parasitemia cases produced significantly higher number of rosettes with human erythrocytes than high parasitemia case isolates (65.5 ± 12.9 and 17.2 ± 5.5, respectively). Interestingly, PkDBPαII of isolates from high parasitemia cases formed significantly larger rosettes with human erythrocytes than PkDBPαII of isolates from low parasitemia cases (18,000 ± 13,000 µm2 and 1,315 ± 623 µm2, respectively).
    Matched MeSH terms: Parasites
  2. The prevalence and trends of canine heartworm (Dirofilaria immitis) in Kuala Lumpur, Malaysia (1970-2018)
    Kunathasan Chelliah M, Šlapeta J
    Vet Parasitol Reg Stud Reports, 2019 04;16:100272.
    PMID: 31027591 DOI: 10.1016/j.vprsr.2019.100272
    Malaysia is considered a hyperendemic area for canine heartworm (Dirofilaria immitis) due to its favorable climate for the completion of the parasite life cycle. This study provides an updated prevalence data on D. immitis in owned dogs from Kuala Lumpur, Malaysia and compares the trends of D. immitis in Malaysia. In the period between December 2017 and June 2018, 3.85% (5/130) dog blood samples tested positive for the presence of D. immitis antigen. A majority of the tested dogs (122/130) were not on rigorous heartworm prevention. After collating and analyzing information from 10 historical studies (1970-2017), we identified a significant decline in prevalence of D. immitis antigen in Malaysia, after the year 2000. Historically, the prevalence of D. immits antigen in owned dogs was significantly lower than the prevalence seen in stray dogs in Malaysia. This study demonstrates that D. immitis remains active in Kuala Lumpur, implying that accurate compliance of heartworm prevention is essential in Malaysia.
    Matched MeSH terms: Parasites
  3. A myopathy associated with protozoan schizonts in chickens in commercial farms in peninsular Malaysia
    Opitz HM, Jakob HJ, Wiensenhuetter E, Devi VV
    Avian Pathol, 1982;11(3):527-34.
    PMID: 18770216
    A myopathy associated with elongated intramuscular protozoan schizonts of uncertain classification was observed in chickens in commercial farms. Of 152 affected fowls originating from 21 flocks in 12 farms, 149 were 24 weeks of age or older and 136 were broiler breeder birds. Both sexes were affected. The disease was only observed during the months of October, November and December, 1976 and 1977. The monthly mortality rate in affected adult flocks rose by 0.5% to 4% and the egg production declined by 5% to 15% during this period. Most affected birds were in good body condition or overweight. Gross lesions were usually present in all skeletal muscles and the cardiac muscle. They resembled nutritional myopathy, sarcosporidiosis, leucocytozoonosis or haemorrhagic syndrome. Microscopically visible elongated schizonts were demonstrated in skeletal muscles and the cardiac muscle in 49 of 55 birds examined histologically. The possible aetiology with respect to known parasites of muscles in fowls is discussed.
    Matched MeSH terms: Parasites
  4. Fulltext COPEPOD AND NEMATODE PARASITES OF THE THREE-SPOTTED DAMSELFISH Dascyllus trimaculatus FROM BIDONGISLAND, TERENGGANU
    PEI YING LEE, YUSRI YUSOF, MELISSA BEATA MARTIN
    UMT Journal of Undergraduate Research, 2021;3(1):7-16.
    MyJurnal
    This study focuses on the parasites in Dascyllus trimaculatus fish in Bidong Island. Though D. trimaculatus is a common reef fish, there is lack on parasites studies of this fish in Malaysia. The objectives of this study are to identify metazoan parasites and analyse the prevalence and mean intensity the parasites in D. trimaculatus. Sampling was randomly conducted on a coral colony of 100 meters within a 15m depth at Pantai Pasir Cina,BidongIsland.ThisresearchresultedintheidentificationofacopepodLernaeocera branchialis and a nematode from the family Camallanidae. The copepods collected in this study infected 20 out of 42 D. trimaculatus specimens, whereas the nematode-like parasites collected infected 18 of out of 42 D. trimaculatus. All copepods found in the gills of D. trimaculatus had a prevalence of 4.95 and mean intensity of 47.62%, while the nematode-like parasite, which were found in the brain, had 2.72 prevalence and 42.86% mean intensity. This elucidates that both parasites are categorised as having light levelbut common frequency of infection, and the current IUCN status of D. trimaculatus has not been reported to be harmedby parasites. Though the mean intensity of both parasites isnot harmful, the prevalence are concerning and might increase in the future, with further incorporation and monitoring of climate change factors that may affect the damselfish.
    Matched MeSH terms: Parasites
  5. Fulltext Helminthic parasites of scavenging chickens (Gallus domesticus) from villages in Penang Island, Malaysia
    Abdul Wahab Rahman, Hasber Salim, Mohd Shafiq Ghause
    Trop Life Sci Res, 2009;20(1):-.
    MyJurnal
    Sixty scavenging chickens from villages in Penang were examined for their state of nutrition or body condition and the presence of helminthic parasites. The majority of the chickens were of poor body condition. More than 66.7% (females) and 73.3% (males) belonged to the ‘below normal’ category of general body condition. A total of eight different helminth species were recovered from the trachea and the gastrointestinal tract of the various chickens. Four of the species were nematodes and the rest were cestodes. No trematodes were recovered from the chickens. The highest mean worm burden was recorded for the cestode Raillietina echinobothrida while the lowest was the nematode, Ascaridia galli.
    Matched MeSH terms: Parasites
  6. Fulltext The effect of macrocyclic lactones-ivermectin exposure on egg hatching and larval development of Caenorhabditis elegans
    Mariani Mohd Zain, Zary Shariman Yahaya, Nik Ahmad Irwan Izzauddin Nik Him
    Trop Life Sci Res, 2016;27(11):3-8.
    MyJurnal
    To date, the ivermectin resistance in nematode parasites has been reported
    and many studies are carried out to determine the causes of this problem. A free-living
    Caenorhabditis elegans is used as a model system for this study to investigate the
    response of C. elegans to ivermectin exposure by using larval development assay. Worms
    were exposed to ivermectin at concentration from 1 ng/mL to 10 ng/mL and dimethyl
    sulphoxide (DMSO) as a control. The developments of the worms were monitored for 24,
    48, 72, and 96 hours until the worms become adults. Results indicated that worms’ growth
    began to be affected by ivermectin at a concentration of 5 ng/mL, while at the
    concentration of 6, 7, 8, 9, and 10 ng/mL, the growth of worms were inhibited compared to
    control worms. Further study of the protein expression in C. elegans should be done to
    investigate the up-regulated and down-regulated proteins involve in ivermectin resistance.
    Matched MeSH terms: Parasites
  7. Fulltext New record of a phoretic flea associated with earwigs (Dermaptera, Arixeniidae) and a redescription of the bat flea Lagaropsylla signata (Siphonaptera, Ischnopsyllidae)
    Hastriter MW, Miller KB, Svenson GJ, Martin GJ, Whiting MF
    Zookeys, 2017.
    PMID: 28331409 DOI: 10.3897/zookeys.657.11095
    Lagaropsylla signata (Wahlgren, 1903), previously known only from the Island of Java, Indonesia is redescribed and reported for the first time in Deer Cave, Gunung Mulu National Park, Sarawak, Malaysia (west coast of Borneo). Many were found clinging to the earwig Arixenia esau Jordan, 1909. A similar account of a phoretic flea (Lagaropsylla turba Smit, 1958) on the same species of cave-dwelling earwig has been reported in peninsular Malaysia in a well-documented association with the hairless naked bulldog bat, Cheiromeles torquatus Horsfield, 1824. The association of Lagaropsylla signata with Arixenia esau is parallel to the evolution and co-existence with bats in Deer Cave just as in the case of Lagaropsylla turba, Arixenia esau, and Cheiromeles torquatus. The evidence suggests that Lagaropsylla turba and Lagaropsylla signata are obligate phoretic parasites whose survival depends on Arixenia esau to access a bat host. Arixenia esau is reported for the first time in Deer Cave and the occurrence of Lagaropsylla signata on the island of Borneo represented a new record, previously being found only on the island of Java. Images of Lagaropsylla signata attached to Arixenia esau are provided. Xeniaria jacobsoni (Burr, 1912), often associated with Arixenia esau in other geographical areas, was not present in the material examined from Deer Cave. The natural history of the earwig genera Arixenia Jordan, 1909 and Xeniaria Maa, 1974 are discussed and summarized relative to their associations with phoretic fleas and their bat hosts.
    Matched MeSH terms: Parasites
  8. Fulltext Maggots from tracheostoma
    Periasamy, Chentilnathan, Irfan Mohamad, Khairul Bariah Johan, Nik Fariza Husna Nik Hassan
    Archives of Orofacial Sciences, 2013;8(1):41-43.
    MyJurnal
    Tracheostomy is a life saving surgical procedure performed to maintain upper airway ventilation. The
    indications include providing relief of upper airway obstruction, to replace endotracheal intubation in patients needing prolonged assisted ventilation, protection of tracheobronchial tree and facilitates tracheobronchial toilet.More importantly, post operative tracheostomy care is very crucial for the success of the treatment and avoiding complications. We report a rare case of an obligate parasite which had infested the tracheal stoma due to poorhygiene.
    Matched MeSH terms: Parasites
  9. Fulltext Immunogenicity of the merozoite surface protein-1 (MSP-1) of human Plasmodium sp.
    Cheong, F.W., Lau, Y.L., Fong, M.Y.
    JUMMEC, 2015;18(2):1-7.
    MyJurnal
    Malaria is a major cause of mortality and morbidity globally. Great efforts have been made in the prevention and the elimination of malaria, especially in controlling the malaria vector, the mosquito. Another promising approach would be the development of malaria vaccines. Malaria vaccine studies can be focused on the pre-erythrocytic-stage antigens and the blood-stage antigens, and on the transmission blocking agents targeting the malaria gametocytes. The blood-stage antigens are the leading candidates in malaria vaccine development, as the blood-stage parasites are responsible for causing symptomatic malaria. Human acquired immunity largely targets on blood-stage antigens. This review focuses on one of the most extensively studied blood-stage antigen, the merozoite surface protein-1 (MSP-1), specifically on its evaluation and immunogenicity in rodents and primate models, and its safety and immunogenicity in human clinical trials.
    Matched MeSH terms: Parasites
  10. Fulltext Plasmodium knowlesi malaria: current research perspectives
    Amir A, Cheong FW, de Silva JR, Liew JWK, Lau YL
    Infect Drug Resist, 2018;11:1145-1155.
    PMID: 30127631 DOI: 10.2147/IDR.S148664
    Originally known to cause simian malaria, Plasmodium knowlesi is now known as the fifth human malaria species. Since the publishing of a report that largely focused on human knowlesi cases in Sarawak in 2004, many more human cases have been reported in nearly all of the countries in Southeast Asia and in travelers returning from these countries. The zoonotic nature of this infection hinders malaria elimination efforts. In order to grasp the current perspective of knowlesi malaria, this literature review explores the different aspects of the disease including risk factors, diagnosis, treatment, and molecular and functional studies. Current studies do not provide sufficient data for an effective control program. Therefore, future direction for knowlesi research is highlighted here with a final aim of controlling, if not eliminating, the parasite.
    Matched MeSH terms: Parasites
  11. Fauna diversity in pitcher plants at Setiam Hill, Bintulu, Sarawak, Malaysia
    Marina M, John Keen C, Caroline B, Afsar J
    Sains Malaysiana, 2018;47:19-25.
    A study was carried out to determine the diversity and enumerate the fauna species related to five pitcher plant species at a selected area in Bukit Setiam Forest, Tatau, Bintulu, Sarawak, Malaysia. At the end of the study, six insect orders together with nematodes and Araneae were detected with different existence abundances and diversity. From the 901 total fauna trapped, 58.82% belonged to the order Hymenoptera, mainly of the ant species, followed by Nematodes (21.64%), Diptera (15.87%), Coleoptera (1.66%), Hemiptera (0.89%), Blattaria (0.44%) and finally, Lepidoptera (0.33%) and Araneae (0.33%). Significant differences (p<0.05) in the composition of insect trapped in pitcher plants were observed for the order Hymenoptera, Diptera, Lepidoptera, Hemiptera and even Nematodes. Meanwhile, no significant difference was observed for Coleoptera, Blattaria and Araneae. There is a strong relationship between fauna and Nepenthes pitcher either as a prey, predator, a mutualistic relationship or parasites or also for a habitat to live or to reproduce.
    Matched MeSH terms: Parasites
  12. Effects of medicated and non-medicated multi-nutrient block supplementation on gastrointestinal parasite infestation and blood hematological parameters of lactating saanen goats
    Mira Panadi, Nor Dini Rusli, Khairiyah Mat, Wan Zahari Mohamed
    Sains Malaysiana, 2018;47:1447-1453.
    In a 90-day feeding trial, twenty four (24) Saanen lactating does were assigned to four treatment group. The treatments
    were feeding: basal diet only as a control (T1), basal diet with urea molasses multi-nutrient block (UMMB) (T2), basal
    diet with medicated urea molasses multi-nutrient block (MUMB) (T3) and basal diet with commercial mineral block (CMB)
    (T4). There were significant differences (p<0.05) between T2, T3 and T4 on the fecal egg count (FEC). Animals in T2
    and T3 showed moderate level of parasite infestation i.e. at 750 epg and 950 epg, respectively, while animals in T1 and
    T4 showed severe parasite load at 4917 epg and 1850 epg, respectively. There were no significant differences (p>0.05)
    between treatments on WBC, LYM, MON, GRA, RBC, HCT, MCV, MCH, PLT, MPV and PCT. However, significant effects (p<0.05)
    on HBG, MCHC, RDW and PDW were observed in T2 and T3. This research showed that UMMB and MUMB were effective
    in controlling parasite infestation in Saanen lactating dairy goats apart from improving their blood hematological
    parameters. Comparison with CMB showed that it is practical to be used for parasite control.
    Matched MeSH terms: Parasites
  13. Comparison of prevalence of intestinal parasitic infections in school children in Kuala Lumpur
    Abdulla MA, Khairul Anuar A, Khalifa S, Salmah I, Suzainur KAR, Mohamad Nazmul HM, et al.
    JUMMEC, 2002;7:118-121.
    A comparative study was carried out to identify the prevalence of intestinal parasitic infestation among students in 3 different types of primary school localed in Ampang area, Kuala Lumpur. A survey of infection rate was done from April 10 June 2001. Stool samples were randomly collected from one·hundred and eleven schoolchildren aged 9-12 years were examined for intestinal parasites, using a light microscope (or direct smear and after concentration for formol-either method. It was found that an overall prevalence of intestinal parasite infection of schoolchildren was 51%. The infection rate of males and females was (60.3%) and (29.2%) respectively. Prevalence seems to be higher in the younger age groups. Also, the usual intestinal parasites were found more commonly in local and foreign schools than international schooL The local school had the highest prevalence (62%), followed by the foreign school (52%) and international school (38.9%). The prevalence in the international school was much lower compared to the local and foreign schools. KEYWORDS: Intestinal parasites, faecal samples, prevalence, schoolchildren
    Matched MeSH terms: Parasites
  14. Fulltext Infection of army ant pupae by two new parasitoid mites (Mesostigmata: Uropodina)
    Brückner A, Klompen H, Bruce AI, Hashim R, von Beeren C
    PeerJ, 2017;5:e3870.
    PMID: 29038753 DOI: 10.7717/peerj.3870
    A great variety of parasites and parasitoids exploit ant societies. Among them are the Mesostigmata mites, a particularly common and diverse group of ant-associated arthropods. While parasitism is ubiquitous in Mesostigmata, parasitoidism has only been described in the genus Macrodinychus. Yet information about the basic biology of most Macrodinychus species is lacking. Out of 24 formally described species, information about basic life-history traits is only available for three species. Here we formally describe two new Macrodinychus species, i.e. Macrodinychus hilpertae and Macrodinychus derbyensis. In both species, immature stages developed as ecto-parasitoids on ant pupae of the South-East Asian army ant Leptogenys distinguenda. By piercing the developing ant with their chelicera, the mites apparently suck ant hemolymph, ultimately killing host individuals. We compare infection rates among all studied Macrodinychus species and discuss possible host countermeasures against parasitoidism. The cryptic lifestyle of living inside ant nests has certainly hampered the scientific discovery of Macrodinychus mites and we expect that many more macrodinychid species await scientific discovery and description.
    Matched MeSH terms: Parasites
  15. Fulltext Migrant Workers in Malaysia: Current Implications of Sociodemographic and Environmental Characteristics in the Transmission of Intestinal Parasitic Infections
    Sahimin N, Lim YA, Ariffin F, Behnke JM, Lewis JW, Mohd Zain SN
    PLoS Negl Trop Dis, 2016 Nov;10(11):e0005110.
    PMID: 27806046 DOI: 10.1371/journal.pntd.0005110
    A cross-sectional study of intestinal parasitic infections amongst migrant workers in Malaysia was conducted. A total of 388 workers were recruited from five sectors including manufacturing, construction, plantation, domestic and food services. The majority were recruited from Indonesia (n = 167, 43.3%), followed by Nepal (n = 81, 20.9%), Bangladesh (n = 70, 18%), India (n = 47, 12.1%) and Myanmar (n = 23, 5.9.2%). A total of four nematode species (Ascaris lumbricoides, Trichuris trichiura, Enterobius vermicularis and hookworms), one cestode (Hymenolepis nana) and three protozoan species (Entamoeba histolytica/dispar, Giardia sp. and Cryptosporidium spp.) were identified. High prevalence of infections with A. lumbricoides (43.3%) was recorded followed by hookworms (13.1%), E. histolytica/dispar (11.6%), Giardia sp. (10.8%), T. trichura (9.5%), Cryptosporodium spp. (3.1%), H. nana (1.8%) and E. vermicularis (0.5%). Infections were significantly influenced by socio-demographic (nationality), and environmental characteristics (length of working years in the country, employment sector and educational level). Up to 84.0% of migrant workers from Nepal and 83.0% from India were infected with intestinal parasites, with the ascarid nematode A. lumbricoides occurring in 72.8% of the Nepalese and 68.1% of the Indian population. In addition, workers with an employment history of less than a year or newly arrived in Malaysia were most likely to show high levels of infection as prevalence of workers infected with A. lumbricoides was reduced from 58.2% to 35.4% following a year's residence. These findings suggest that improvement is warranted in public health and should include mandatory medical screening upon entry into the country.
    Matched MeSH terms: Parasites/classification; Parasites/genetics; Parasites/isolation & purification*
  16. Fulltext A Review of Quercus infectoria (Olivier) Galls as a Resource for Anti-parasitic Agents: In Vitro and In Vivo Studies
    Zin NNINM, Rahimi WNAWM, Bakar NA
    Malays J Med Sci, 2019 Nov;26(6):19-34.
    PMID: 31908584 MyJurnal DOI: 10.21315/mjms2019.26.6.3
    Parasitic diseases represent one of the causes for significant global economic, environmental and public health impacts. The efficacy of currently available anti-parasitic drugs has been threatened by the emergence of single drug- or multidrug-resistant parasite populations, vector threats and high cost of drug development. Therefore, the discovery of more potent anti-parasitic drugs coming from medicinal plants such as Quercus infectoria is seen as a major approach to tackle the problem. A systematic review was conducted to assess the efficacy of Q. infectoria in treating parasitic diseases both in vitro and in vivo due to the lack of such reviews on the anti-parasitic activities of this plant. This review consisted of intensive searches from three databases including PubMed, Science Direct and Scopus. Articles were selected throughout the years, limited to English language and fully documented. A total of 454 potential articles were identified, but only four articles were accepted to be evaluated based on inclusion and exclusion criteria. Although there were insufficient pieces of evidence to account for the efficacy of Q. infectoria against the parasites, this plant appears to have anti-leishmanial, anti-blastocystis and anti-amoebic activities. More studies in vitro and in vivo are warranted to further validate the anti-parasitic efficacy of Q. infectoria.
    Matched MeSH terms: Parasites
  17. Fulltext Attenuation of hydrogen peroxide and ferric reducing/antioxidant power serum levels in colorectal cancer patients with intestinal parasitic infection
    Chandramathi S, Suresh KG, Anita ZB, Kuppusamy UR
    Malays J Med Sci, 2009 Apr;16(2):15-20.
    PMID: 22589653 MyJurnal
    This study assessed several common oxidative indices in subjects infected with intestinal parasites, as well as in colorectal cancer (CRC) patients both with and without intestinal parasites.
    Matched MeSH terms: Parasites
  18. Waterborne parasites and physico-chemical assessment of selected lakes in Malaysia
    Onichandran S, Kumar T, Lim YA, Sawangjaroen N, Andiappan H, Salibay CC, et al.
    Parasitol Res, 2013 Dec;112(12):4185-91.
    PMID: 24046263 DOI: 10.1007/s00436-013-3610-1
    The objective of this study was to assess the physico-chemical parameters and waterborne parasites in selected recreational lakes from Malaysia. Samples were collected from seven stations of Recreational Lake A (RL-A) and six stations of Recreational Lake B (RL-B). The samples were processed to detect the presence of Giardia spp. and Cryptosporidium spp. using immunomagnetic separation kit, helminth eggs or ova by bright field microscopy and Acanthamoeba spp. by cultivation in non-nutrient agar. Chemical parameters such as ammonia, chlorine, fluoride, nitrate and nitrite and physical parameters such as dissolved oxygen, electrical conductivity, pH, salinity, temperature and total dissolved solid were also measured. Both lakes were freshwater with salinity ranging from 0.05 to 0.09 ppt. Most stations of these lakes were contaminated with Cryptosporidium spp., Giardia spp., Ascaris spp. and hookworm. Schistosoma spp. was found in RL-B only, while Acanthamoeba spp. was found in all stations. Of all sampling sites, station 5 of RL-B is the most contaminated. Linear regression and correlation analysis revealed that Giardia spp. and Schistosoma spp. showed a significant negative correlation with turbidity (p 
    Matched MeSH terms: Parasites/isolation & purification*
  19. Fulltext Survival Rate and Transcriptional Response upon Infection with the Generalist Parasite Beauveria bassiana in a World-Wide Sample of Drosophila melanogaster
    Paparazzo F, Tellier A, Stephan W, Hutter S
    PLoS One, 2015;10(7):e0132129.
    PMID: 26154519 DOI: 10.1371/journal.pone.0132129
    The ability to cope with infection by a parasite is one of the major challenges for any host species and is a major driver of evolution. Parasite pressure differs between habitats. It is thought to be higher in tropical regions compared to temporal ones. We infected Drosophila melanogaster from two tropical (Malaysia and Zimbabwe) and two temperate populations (the Netherlands and North Carolina) with the generalist entomopathogenic fungus Beauveria bassiana to examine if adaptation to local parasite pressures led to differences in resistance. Contrary to previous findings we observed increased survival in temperate populations. This, however, is not due to increased resistance to infection per se, but rather the consequence of a higher general vigor of the temperate populations. We also assessed transcriptional response to infection within these flies eight and 24 hours after infection. Only few genes were induced at the earlier time point, most of which are involved in detoxification. In contrast, we identified more than 4,000 genes that changed their expression state after 24 hours. This response was generally conserved over all populations with only few genes being uniquely regulated in the temperate populations. We furthermore found that the American population was transcriptionally highly diverged from all other populations concerning basal levels of gene expression. This was particularly true for stress and immune response genes, which might be the genetic basis for their elevated vigor.
    Matched MeSH terms: Parasites/physiology*
  20. Antimicrobial properties of multifunctional polypyrrole-cobalt oxide-silver nanocomposite against pathogenic bacteria and parasite
    Masri A, Abdelnasir S, Anwar A, Iqbal J, Numan A, Jagadish P, et al.
    Appl Microbiol Biotechnol, 2021 Apr;105(8):3315-3325.
    PMID: 33797573 DOI: 10.1007/s00253-021-11221-1
    BACKGROUND: Conducting polymer based nanocomposites are known to be effective against pathogens. Herein, we report the antimicrobial properties of multifunctional polypyrrole-cobalt oxide-silver nanocomposite (PPy-Co3O4-AgNPs) for the first time. Antibacterial activities were tested against multi-drug-resistant Gram-negative Escherichia coli (E. coli) and Gram-positive methicillin-resistant Staphylococcus aureus (MRSA) bacteria, while antiamoebic effects were assessed against opportunistic protist Acanthamoeba castellanii (A. castellanii).

    RESULTS: The ternary nanocomposite containing conducting polymer polypyrrole, cobalt oxide, and silver nanoparticles showed potent antimicrobial effects against these pathogens. The antibacterial assay showed that PPy-Co3O4-AgNPs exhibited significant bactericidal activity against neuropathogenic E. coli K1 at only 8 μg/mL as compared to individual components of the nanocomposite, whereas a 70 % inhibition of A. castellanii viability was observed at 50 μg/mL. Moreover, PPy-Co3O4-AgNPs were found to have minimal cytotoxicity against human keratinocytes HaCaT cells in vitro even at higher concentration (50 μg/mL), and also reduced the microbes-mediated cytopathogenicity against host cells.

    CONCLUSION: These results demonstrate that PPy-Co3O4-AgNPs hold promise in the development of novel antimicrobial nanomaterials for biomedical applications.

    KEY POINTS: •Synthesis of polypyrrole-cobalt oxide-silver (PPy-Co3O4-AgNPs) nanocomposite. •Antimicrobial activity of nanocomposite. •PPy-Co3O4-AgNPs hold promise for biomedical applications.

    Matched MeSH terms: Parasites*
Related Terms
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links