The treatment of skin and soft tissue infections caused by methicillin-resistant Staphylococcus aureus (MRSA) remains a challenge, partly due to localization of the bacteria inside the host's cells, where antimicrobial penetration and efficacy is limited. We formulated the cationic polymer polyhexamethylene biguanide (PHMB) with the topical antibiotic nadifloxacin and tested the activities against intracellular MRSA in infected keratinocytes. The PHMB/nadifloxacin nanoparticles displayed a size of 291.3 ± 89.6 nm, polydispersity index of 0.35 ± 0.04, zeta potential of +20.2 ± 4.8 mV, and drug encapsulation efficiency of 58.25 ± 3.4%. The nanoparticles killed intracellular MRSA, and relative to free polymer or drugs used separately or together, the nanoparticles displayed reduced toxicity and improved host cell recovery. Together, these findings show that PHMB/nadifloxacin nanoparticles are effective against intracellular bacteria and could be further developed for the treatment of skin and soft tissue infections.
We have determined the protective effects of Thymus serpyllum (TS) extract and nanoparticle-loaded TS on hydrogen peroxide-induced cell death of mesenchymal stromal cells (MSCs) in vitro. Gas chromatography-mass spectroscopy confirmed the spectrum of active components in the extract. Out of the three different extracts, the hexane extract showed significant free radical scavenging activity. Treatment of MSCs with H2O2 (hydrogen peroxide) significantly increased intracellular cell death; however, pretreatment with TS extract and nanoparticle-loaded TS (200 μg/ml) suppressed H2O2-induced elevation of Cyt-c and MMP13 and increased the survival rates of MSCs. H2O2-induced (0.1 mM) changes in cytokines were attenuated in the extract and nanoparticles by pretreatment and cotreatment at two time points (p < 0.05). H2O2 increased cell apoptosis. In contrast, treatment with nanoparticle-loaded TS suppressed the percentage of apoptosis considerably (p < 0.05). Therefore, TS may be considered as a potential candidate for enhancing the effectiveness of MSC transplantation in cell therapy.
The aim was to explore the utilization of oil palm mesocarp fiber (OPMF) as a source for the production of cellulose nanocrystals (CNC). OPMF was first treated with alkali and then bleached before the production of CNC by acid hydrolysis (H₂SO₄). The produced materials were characterized using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), a scanning electron microscope (SEM) and a transmission electron microscope (TEM). It was proven that acid hydrolysis can increase the crystallinity of bleached OPMF and reduce the dimension of cellulose to nano scale. Changes in the peaks of the FTIR spectrum at 2852 (C-H stretching), 1732 (C=O stretching) and 1234 cm-1 (C-O stretching) indicated that the alkali treatment completely removed hemicelluloses and lignin from the fiber surface. This can be seen from the thermogram obtained from the TGA characterization. Morphological characterization clearly showed the formation of rod-shaped CNCs. The promising results prove that OPMF is a valuable source for the production of CNC.
Diosgenin encapsulated PCL-Pluronic nanoparticles (PCL-F68-D-NPs) were developed using the nanoprecipitation method to improve performance in brain cancer (glioblastoma) therapy. The nanoparticles were characterized by dynamic light scattering (DLS)/Zeta potential, Fourier-transform infrared (FTIR) spectra, X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Transmission electron microscopy (TEM). The encapsulation efficiency, loading efficiency, and yield were calculated. The in vitro release rate was determined, and the kinetic model of diosgenin release was plotted and ascertained. The cytotoxicity was checked by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)assay against U87-MG cells (glioblastoma cell lines). The obtained nanoparticles demonstrated good size distribution, stability, morphology, chemical, and mechanical properties. The nanoparticles also possessed high encapsulation efficiency, loading efficiency, and yield. The release rate of Diosgenin was shown in a sustained manner. The in vitro cytotoxicity of PCL-F68-D-NPs showed higher toxicity against U87-MG cells than free Diosgenin.
Introduction: Application of nano-engineered fingerprint dusting powders has been a recent trend to achieve latent fingermark development with superior ridge clarity. As such, efforts have been made to utilise natural resources to increase the sustainability of these emerging nano-engineered powders. Lithium-doped zinc oxide, primarily used as white pigments, have been previously applied to latent fingermarks with success. In the current study, nanostruc- tured zinc oxide, synthesised using neem extract as the reducing agent, was evaluated for fingermark development on non-porous surfaces. Methods: The reduction of zinc nitrate hexahydrate was facilitated by neem extract, pre- pared by boiling neem leaves in distilled water. The thick yellow paste recovered was calcined in the furnace to produce a light yellow powder. Physicochemical composition of the powder was determined using microscopic and spectroscopic instruments. The effectiveness of the powder was tested on natural fingermark deposited on several non-porous surfaces. Results: Nanostructured zinc oxide with particle size ranging in between 1 to 3 µm consisting of highly aggregated spherical particle with less than 100 nm dimensions were synthesised. Developed fingermarks revealed excellent ridge details and contrast on dark coloured surfaces. Studying the fingermark closely under scan- ning electron microscope displayed selective distribution of particle on the ridges of the fingermark residue and very minimal deposition on the fingermark valleys. Conclusion: Nanostructured zinc oxide fabricated using green chem- istry approach can be applied for the development of fingermark. Nevertheless, future works can be undertaken to enhance particle dispersity and to confer strong photoluminescence to the zinc oxide nanoparticles.
Asymmetric polyphenylsulfone (PPSU) membranes were fabricated by a non-solvent induced phase inversion method. Glycerin and silica nanoparticles were added into the polymer solution to investigate their effects on the material properties and gas separation performance of prepared membranes. The morphology and structure of PPSU membranes were analyzed by scanning electron microscopy (SEM), the surface roughness of the selective layer was analyzed by atomic force microscopy (AFM), and the surface free energy was calculated based on the contact angle measurements by using various solvents. The gas separation performance of PPSU membranes was estimated by measuring the permeability of CO2 and CH4. The addition of glycerin as a nonsolvent into the polymer solution changed the cross-section structure from finger-like structure into sponge-like structure due to the delayed liquid-liquid demixing process, which was confirmed by SEM analysis. The incorporation of silica nanoparticles into PPSU membranes slightly increased the hydrophilicity, which was confirmed by water contact angle results. PPSU membrane fabricated from the polymer solution containing 10 wt.% glycerin showed the best CO2/CH4 selectivity of 3.86 and the CO2 permeability of 1044.01 Barrer. Mixed matrix PPSU membrane containing 0.1 wt.% silica nanoparticles showed the CO2/CH4 selectivity of 3.16 and the CO2 permeability of 1202.77 Barrer.
Colorectal cancer (CRC) is a global public health issue which poses a substantial humanistic and economic burden on patients, healthcare systems and society. In recent years, intestinal dysbiosis has been suggested to be involved in the pathogenesis of CRC, with specific pathogens exhibiting oncogenic potentials such as Fusobacterium nucleatum, Escherichia coli and enterotoxigenic Bacteroides fragilis having been found to contribute to CRC development. More recently, it has been shown that initiation of CRC development by these microorganisms requires the formation of biofilms. Gut microbial biofilm forms in the inner colonic mucus layer and is composed of polymicrobial communities. Biofilm results in the redistribution of colonic epithelial cell E-cadherin, increases permeability of the gut and causes a loss of function of the intestinal barrier, all of which enhance intestinal dysbiosis. This literature review aims to compile the various strategies that target these pathogenic biofilms and could potentially play a role in the prevention of CRC. We explore the potential use of natural products, silver nanoparticles, upconverting nanoparticles, thiosalicylate complexes, anti-rheumatic agent (Auranofin), probiotics and quorum-sensing inhibitors as strategies to hinder colon carcinogenesis via targeting colon-associated biofilms.
This work aims to develop corn starch/chitosan nanoparticles/thymol (CS/CNP/Thy) bio-nanocomposite films as potential food packaging materials that can enhance the shelf life of food. CS/CNP/Thy bio-nanocomposite films were prepared by the addition of different concentrations of thymol (0, 1.5, 3.0, 4.5 w/w%) using a solvent casting method. The resulting films were characterized in terms of optical, mechanical, and water vapor permeability (WVP) properties. The addition of thymol was found to reduce the tensile strength (TS), elongation at break (EAB), and Young's modulus (YM) of the films. Generally, the increment in the concentration of thymol did not significantly affect the TS, EAB, and YM values. The addition of 1.5 w/w% thymol increased the WVP of the films but the WVP reduced with the increase in thymol concentrations. CS/CNP/Thy-3% bio-nanocomposite films demonstrated the potential to lengthen the shelf life of cherry tomatoes packed with the films, whereby the cherry tomatoes exhibited no significant changes in firmness and the lowest weight loss. In addition, no mold growth was observed on the sliced cherry tomatoes that were in direct contact with the films during 7 days of storage, proving the promising application of the films as active food packaging materials.
Carbon nanoparticles have been widely used in various applications. However, they are commonly known to have low dispersibility and chemical inertness which limit their practical ability in medical or biological area. Some studies have been performed to modify carbon nanoparticles such as carbon nanotubes using ultraviolet (UV)-Ozone system. However, little is known on the effects of such system towards other types of carbon nanoparticles such as mesoporous hollow carbon nanoparticles (MHCNs). Thus, in this study, improvement of MHCNs physiochemical properties have been studied using UV-Ozone treatment for the first time. The treatment was conducted in water as dispersant agent at ozone flowrate of 1.0 L/min and exposure time of 45 min. SEM images observed that MHCNs morphology and surface structure remain intact after the treatment. Observations on the dispersibility of MHCNs in phosphate buffered saline (PBS) solution shows that the dispersibility was improved compared to the untreated ones. This was supported by the low Z-average and PDI values of treated MHCNs obtained at ~400 nm and 0.2, respectively when compared to the untreated MHCNs which was obtained at 970 nm and 0.417, respectively. Thermogravimetric analysis (TGA) showed an increased in weight loss of treated MHCNs at the lower temperature compared to untreated MHCNs. Results from Fourier Transform Infrared (FTIR) showed an increase number of new functional groups that includes carboxylic acid group presence at the surface of treated MHCNs which contributes to the improvement of their dispersibility, thermal properties and chemical functionality. These findings opened a new possibility of using UV-Ozone treatment to improve physicochemical properties of MHCNs for medical area such as in drug delivery application in addition to their excellent storage and carrier system.
Impacts of gold nanoparticles on MHD Poiseuille flow of nanofluid in a porous medium are studied. Mixed convection is induced due to external pressure gradient and buoyancy force. Additional effects of thermal radiation, chemical reaction and thermal diffusion are also considered. Gold nanoparticles of cylindrical shape are considered in kerosene oil taken as conventional base fluid. However, for comparison, four other types of nanoparticles (silver, copper, alumina and magnetite) are also considered. The problem is modeled in terms of partial differential equations with suitable boundary conditions and then computed by perturbation technique. Exact expressions for velocity and temperature are obtained. Graphical results are mapped in order to tackle the physics of the embedded parameters. This study mainly focuses on gold nanoparticles; however, for the sake of comparison, four other types of nanoparticles namely silver, copper, alumina and magnetite are analyzed for the heat transfer rate. The obtained results show that metals have higher rate of heat transfer than metal oxides. Gold nanoparticles have the highest rate of heat transfer followed by alumina and magnetite. Porosity and magnetic field have opposite effects on velocity.
Friction and wear are the main factors in the failure of the piston in automobile engines. The objective of this work was to improve the tribological behaviour and lubricant properties using hybrid Cellulose Nanocrystal (CNC) and Copper (II) oxide nanoparticles blended with SAE 40 as a base fluid. The two-step method was used in the hybrid nanofluid preparation. Three different concentrations were prepared in a range of 0.1% to 0.5%. Kinematic viscosity and viscosity index were also identified. The friction and wear behavior were evaluated using a tribometer based on ASTM G181. The CNC-CuO nano lubricant shows a significant improvement in term of viscosity index by 44.3-47.12% while for friction, the coefficient of friction (COF) decreases by 1.5%, respectively, during high and low-speed loads (boundary regime), and 30.95% during a high-speed, and low load (mixed regime). The wear morphologies results also show that a smoother surface was obtained after using CNC-CuO nano lubricant compared to SAE 40.
Chitosan nanoparticles are exhalation prone and agglomerative to pulmonary inhalation. Blending nanoparticles with lactose microparticles (∼5 µm) could mutually reduce their agglomeration through surface adsorption phenomenon. The chitosan nanoparticles of varying size, size distribution, zeta potential, crystallinity, shape and surface roughness were prepared by spray drying technique as a function of chitosan, surfactant and processing conditions. Lactose-polyethylene glycol 3000 (PEG3000) microparticles were similarly prepared. The chitosan nanoparticles, physically blended with fine lactose-PEG3000 microparticles, exhibited a comparable inhalation performance with the commercial dry powder inhaler products (fine particle fraction between 20% and 30%). Cascade impactor analysis indicated that the aerosolization and inhalation performance of chitosan nanoparticles was promoted by their higher zeta potential and circularity, and larger size attributes of which led to reduced inter-nanoparticulate aggregation and favored nanoparticles interacting with lactose-PEG3000 micropaticles that aided their delivery into deep and peripheral lungs.
Synthesis of nanoparticles by using plant have sparked interest among researchers due to environmentally safe, inexpensive and simple method to compare with chemical method. Use of plant in synthesis zinc oxide nanoparticles (ZnO NPs) that act as reducing and capping agent are more recommended, due to high production of product and rate of synthesis is faster than using microorganism. This study focus on the synthesis of ZnO NPs by using leaf extract of aloe vera (Aloe bardenisis miller) with different concentration (30%, 40% and 50%) and various calcination temperature which are 500 ˚C, 700 ˚C and 900 ˚C for 4 hours. Fourier – transform infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), scanning electron microscopy (SEM), X-ray Diffraction (XRD) and Brunauer-Emmet and Teller (BET) were used to characterize the prepared samples. FTIR spectra showed present wavenumber in between 400-500 cm-1 indicated the presence of Zn-O stretch. Powder XRD pattern confirmed the hexagonal wurtzite structure with average particles size from 24.19 nm to 67.69 nm for all concentration and temperature by using Scherer’s equation. For SEM analysis the images show irregular shape for concentrations 30% and 50% with size range from 500 nm to 900 nm while for concentration 40% cubic shape was observe with size range from 140 nm to 900 nm. All characterize show that formation of ZnO NPs depend on the concentration and calcination temperature. Sample 30% and 50% ZnO NPs was applied in lithium battery at voltage from 0.01 to 3. 1.2 mAhg-1 was recorded for sample 30% ZnO NPs while 100 mAhg-1.
Semiconductor oxides such as titanium dioxide (TiO2) and zinc oxide (ZnO) are used as the photocatalyst for removing contaminants. In addition, TiO2 and ZnO nanoparticles in the suspension form makes it difficult to be recovered and recycled. This study was conducted to investigate the efficiency of immobilizing TiO2 and ZnO nanoparticles in epoxy beads. The immobilization process using different ratios of photocatalysts TiO2/ZnO (1:0, 3:1, 1:1, 1:3 and 0:1) fixed on epoxy material. These epoxy beads were used for dye removal in photocatalysis using methylene blue (MB) solution at a concentration of 10mg/L. Besides, epoxy beads also characterized using scanning electron microscope (SEM), attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy and thermogravimetric analysis (TGA). The results showed that the highly recommended epoxy bead is 3:1 ratio of TiO2/ZnO because it has good performance in dye degradation that proved from reducing concentration of MB to 2.4mg/L (76%). However, TiO2/ZnO characterization of 3:1 by SEM show on the surface the particle are found to be spherical in shape which is relatively high efficiency for the degradation, ATR-FTIR pattern in broad band 4000 cm-1 - 400cm-1 which correspond to hydroxyl stretching to be adsorbed at peak (474.49 cm-1 - 3722.61cm-1) respectively to the optimum for the degradation and TGA rate of change are 5mg to 2.5mg that residue (49.78%) due to decomposition or oxidation from mass loss. These findings are very effective and economical technique to be cost saving and highly efficient photocatalyst.
Plastics packaging is non-biodegradable and risks to human health and environmental pollution. In contrast, gelatin-based film lack of desired mechanical, thermal and water vapour barrier properties. Thus, this study aimed to investigate the mechanical and physical properties of bio-nanocomposite films based on chicken skin gelatin with different concentration of chitosan nanoparticles (CSNPs). Gelatin/CSNPs film solutions with different CSNPs concentration (0-8%, w/w) were stirred at 45oC for 30 min and oven-dried at 45oC. Film characterization determination includes tensile strength (TS), elongation at break (EAB), Young’s modulus (YM), water solubility, water vapour permeability (WVP), film morphology and melting temperature (Tm). Results of the study indicated that incorporation of CSNPs significantly influenced film properties. The addition of CSNPs increased the TS and YM value, which lead to stronger films than the pure chicken skin gelatin films. However the addition of CSNPs decreased the EAB value. Furthermore, WVP and water solubility significantly decreased (p < 0.05) by the addition of 6% CSNPs. Morphology images showed that increased CSNPs reduced the film’s amorphous character, especially in high level, in which higher CSNPs (8%) resulted in the aggregation of particles in the composites. The nano-reinforcement films showed higher thermal stability as compared to pure chicken skin gelatin films. In conclusion, the film with 6% CSNPs showed the best formulation, as it demonstrated high in TS, YM and Tm value, while low in EAB, water solubility and WVP value compared to other films. The results presented in this study showed the feasibility of using bio-nanocomposite technology to improve the properties of biopolymer films based on chicken skin gelatin.
Over recent years, keratin has gained great popularity due to its exceptional biocompatible and biodegradable nature. It has shown promising results in various industries like poultry, textile, agriculture, cosmetics, and pharmaceutical. Keratin is a multipurpose biopolymer that has been used in the production of fibrous composites, and with necessary modifications, it can be developed into gels, films, nanoparticles, and microparticles. Its stability against enzymatic degradation and unique biocompatibility has found their way into biomedical applications and regenerative medicine. This review discusses the structure of keratin, its classification and its properties. It also covers various methods by which keratin is extracted like chemical hydrolysis, enzymatic and microbial treatment, dissolution in ionic liquids, microwave irradiation, steam explosion technique, and thermal hydrolysis or superheated process. Special emphasis is placed on its utilisation in the form of hydrogels, films, fibres, sponges, and scaffolds in various biotechnological and industrial sectors. The present review can be noteworthy for the researchers working on natural protein and related usage.
A rapid, sustainable, and ecologically sound approach is urgently needed for the production of semiconductor nanomaterials. CuSe nanoparticles (NPs) were synthesized via a microwave-assisted technique using CuCl2·2H2O and Na2SeO3 as the starting materials. The role of the irradiation time was considered as the primary concern to regulate the size and possibly the shape of the synthesized nanoparticles. A range of characterization techniques was used to elucidate the structural and optical properties of the fabricated nanoparticles, which included X-ray diffraction, energy-dispersive X-ray spectroscopy (EDX), atomic force microscopy, field emission scanning electron microscopy, Raman spectroscopy (Raman), UV-Visible diffuse reflectance spectroscopy (DRS), and photoluminescence spectroscopy (PL). The mean crystallite size of the CuSe hexagonal (Klockmannite) crystal structure increased from 21.35 to 99.85 nm with the increase in irradiation time. At the same time, the microstrain and dislocation density decreased from 7.90 × 10-4 to 1.560 × 10-4 and 4.68 × 10-2 to 1.00 × 10-2 nm-2, respectively. Three Raman vibrational bands attributed to CuSe NPs have been identified in the Raman spectrum. Irradiation time was also seen to play a critical role in the NP optical band gap during the synthesis. The decrease in the optical band gap from 1.85 to 1.60 eV is attributed to the increase in the crystallite size when the irradiation time was increased. At 400 nm excitation wavelength, a strong orange emission centered at 610 nm was observed from the PL measurement. The PL intensity is found to increase with an increase in irradiation time, which is attributed to the improvement in crystallinity at higher irradiation time. Therefore, the results obtained in this study could be of great benefit in the field of photonics, solar cells, and optoelectronic applications.
Multidrug resistant bacteria create a challenging situation for society to treat infections. Multidrug resistance (MDR) is the reason for biofilm bacteria to cause chronic infection. Plant-based nanoparticles could be an alternative solution as potential drug candidates against these MDR bacteria, as many plants are well known for their antimicrobial activity against pathogenic microorganisms. Spondias mombin is a traditional plant which has already been used for medicinal purposes as every part of this plant has been proven to have its own medicinal values. In this research, the S. mombin extract was used to synthesise AgNPs. The synthesized AgNPs were characterized and further tested for their antibacterial, reactive oxygen species and cytotoxicity properties. The characterization results showed the synthesized AgNPs to be between 8 to 50 nm with -11.52 of zeta potential value. The existence of the silver element in the AgNPs was confirmed with the peaks obtained in the EDX spectrometry. Significant antibacterial activity was observed against selected biofilm-forming pathogenic bacteria. The cytotoxicity study with A. salina revealed the LC50 of synthesized AgNPs was at 0.81 mg/mL. Based on the ROS quantification, it was suggested that the ROS production, due to the interaction of AgNP with different bacterial cells, causes structural changes of the cell. This proves that the synthesized AgNPs could be an effective drug against multidrug resistant bacteria.
The effect of incorporating different loadings of oil palm bio-ash nanoparticles from agriculture waste on the properties of phenol-formaldehyde resin was investigated in this study. The bio-ash filler was used to enhance the performance of phenol-formaldehyde nanocomposites. Phenol-formaldehyde resin filled with oil palm bio-ash nanoparticles was prepared via the in-situ polymerization process to produce nanocomposites. The transmission electron microscope and particle size analyzer result revealed that oil palm bio-ash nanoparticles had a spherical geometry of 90 nm. Furthermore, X-ray diffraction results confirmed the formation of crystalline structure in oil palm bio-ash nanoparticles and phenol-formaldehyde nanocomposites. The thermogravimetric analysis indicated that the presence of oil palm bio-ash nanoparticles enhanced the thermal stability of the nanocomposites. The presence of oil palm bio-ash nanoparticles with 1% loading in phenol-formaldehyde resin enhanced the internal bonding strength of plywood composites. The scanning electron microscope image revealed that phenol-formaldehyde nanocomposites morphology had better uniform distribution and dispersion with 1% oil palm bio-ash nanoparticle loading than other phenol-formaldehyde nanocomposites produced. The nanocomposite has potential use in the development of particle and panel board for industrial applications.
In this work, a simple, co-precipitation technique was used to prepare un-doped, pure tin oxide (SnO₂). As synthesized SnO₂ nanoparticles were doped with Cu2+ ions. Detailed characterization was carried out to observe the crystalline phase, morphological features and chemical constituents with opto-electrical and magnetic properties of the synthesized nanoparticles (NPs). X-ray diffraction analysis showed the existence of crystalline, tetragonal structure of SnO₂. Both the sample synthesized here showed different crystalline morphology. The band gap energy (Eg) of the synthesized sample was estimated and it was found to decrease from 3.60 to 3.26 eV. The band gap energy reduced due to increase in Cu2+ dopant amount inside the SnO₂ lattice. Optical properties were analyzed using absorption spectra and Photoluminescence (PL) spectra. It was observed that Cu2+ ions incorporated SnO₂ NPs exhibited more degradation efficiencies for Rhodamine B (RhB) dye compared to un-doped sample under UV-Visible irradiation. The dielectric characteristics of un-doped, pure and Cu2+ incorporated SnO₂ nanoparticles were studied at different frequency region under different temperatures. The ac conductivity and impedance analysis of pure and Cu2+ incorporated SnO₂ nanoparticles was also studied. The magnetic properties of the synthesized samples were analysed. Both the sample showed ferromagnetic properties. The research indicated that the Cu2+ ions doping can make the sample a promising candidate for using in the field of optoelectronics, magneto electronics, and microwave devices.