Displaying publications 81 - 100 of 648 in total

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  1. Kabyl A, Yang M, Shah D, Ahmad A
    Int J Environ Res Public Health, 2022 Nov 17;19(22).
    PMID: 36429909 DOI: 10.3390/ijerph192215190
    Oil spills are environmental pollution events that occur due to natural disasters or human activities, resulting in a liquid petroleum hydrocarbon release in the environment, especially into the marine ecosystem. Once oil spills happen, they cause detrimental consequences to the environment, living organisms, and humans. Although there are increasing oil and gas activities in the Arctic region, which is abundant with undiscovered oil and gas resources, the harsh environmental conditions of the region, such as the ice coverage, cold temperatures, long periods of darkness, and its remoteness, pose significant challenges to managing the risk of accidental oil spills in ice-infested waters. In this paper, a bibliometric analysis has been applied to study the global work on oil spill research in ice-infested waters. The paper aims to present an overview of the available oil spill response methods in ice-infested waters, identify the current trends of the research on oil spills in ice-infested waters, and determine the challenges with the future research directions based on the bibliometric analysis. The analysis includes a total number of 77 articles that have been published in this research field which were available in the Scopus database, involving 193 authors from 17 countries dating from 1960 to September 2022. During the bibliometric analysis, the top five most productive authors and countries as well as the most cited publications on oil spills in ice-infested waters have been identified; the authors' cooperation network and the cooperation network between the countries in oil spills research in ice-infested waters have been created; a co-citation analysis and a terms analysis have been performed to identify the popular terms and topics. For future directions, it is recommended for researchers (1) to study real oil spills as much as possible to obtain a good overview through replication under different situations; (2) to develop a new technique for the careful examination and management of the potential risks; (3) to study oil separation from the recovered oil-ice mixture.
  2. Nazar M, Ahmad A, Hussain SMS, Moniruzzaman M
    Mar Pollut Bull, 2024 Apr 02;202:116311.
    PMID: 38574502 DOI: 10.1016/j.marpolbul.2024.116311
    The synthesis of new surfactants helps to mitigate the environmental and financial effects of oil spills by providing efficient cleanup options. Herein, this study provides the development of a binary mixture of Span 80 and Choline myristate [Cho][Mys], a surface-active ionic liquid (SAIL) as green dispersant for oil spill remediation. The synergistic interaction at a 60:40 (w/w) ratio significantly lowered the critical micelle concentration (cmc) to 0.029 mM. Dispersion efficiency tests with Arab crude oil showed optimal performance at a 60:40 ratio of Span 80 and [Cho][Mys] (1:25 dispersant to oil ratio, v/v), achieving 81.16 % dispersion effectiveness in the baffled flask test. The binary mixture demonstrated superior emulsion stability (6 h) and the lowest interfacial tension (1.12 mN/m). Acute toxicity experiments revealed the dispersant's practical non-toxicity with an LC50 value of 600 mg/L. Overall, this environmentally benign surfactant combination shows promise as a safe and effective oil spill dispersant.
  3. Benhamou K, Kaddami H, Magnin A, Dufresne A, Ahmad A
    Carbohydr Polym, 2015 May 20;122:202-11.
    PMID: 25817660 DOI: 10.1016/j.carbpol.2014.12.081
    Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation. Two types of nanofiber were used: cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs). The mechanical and thermal properties of the nanocomposite films were studied by DMA, DSC, and tensile tests and the morphology was investigated by SEM. Bionanocomposites presented good mechanical properties in comparison to neat PU. While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix. Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
  4. Ahmad A, Omar KM, Alahmadi AA, Rizg WY, Bairwan RD, Abdul Khalil HPS
    Int J Biol Macromol, 2024 Feb;258(Pt 1):128746.
    PMID: 38104681 DOI: 10.1016/j.ijbiomac.2023.128746
    Due to growing environmental concerns for better waste management, this study proposes developing a composite aerogel using cellulose nanofibers (CNF) and spent coffee grounds (SCG) through an eco-friendly method for efficient methylene blue (MB) adsorption. Adding SCG to the CNF aerogel altered the physical properties: it increases the volume (4.14 cm3 to 5.25 cm3) and density (0.018 to 0.022 g/cm3) but decrease the water adsorption capacity (2064 % to 1635 %). FTIR spectrum showed distinct functional groups in both all aerogels, showing hydroxyl, glyosidic bonds, and aromatic compounds. Additionally, SCG improved thermal stability of the aerogels. In term of adsorption efficacy, CNF-SCG40% aerogel as exceptionally well. According to Langmuir isotherm models, the adsorption of MB happened in a monolayer, with CNF-SCG40% showing a maximum adsorption capacity of 113.64 mg/g, surpassing CNF aerogel (58.82 mg/g). The study identified that the pseudo-second-order model effectively depicted the adsorption process, indicating a chemical-like interaction. This investigation successfully produced a single-use composite aerogel composed of CNF and SCG using an eco-friendly approach, efficiently adsorbing MB. By utilizing cost-effective materials and eco-friendly methods, this approach offers a sustainable solution for waste management, contributes to an eco-friendly industrial environment, and reduces production expenses and management costs.
  5. Ahmad A, Ghufran R, Al-Hosni TK
    J Environ Health Sci Eng, 2019 Dec;17(2):1195-1203.
    PMID: 32030185 DOI: 10.1007/s40201-019-00434-2
    To investigate the interaction of zinc oxide nanoparticles (ZnO NPs) with fly ash soil (FAS) for the reduction of metals from FAS by Parthenium hysterophorus were studied. The average accumulation of metals by P. hysterophorus stem were Fe 79.6%; Zn 88.5%; Cu 67.5%; Pb 93.6%; Ni 43.5% and Hg 39.4% at 5.5 g ZnO NP. The concentration of ZnO NP at 1.5 g did not affect the metals accumulation, however at 5.5 g ZnO NP showed highest metal reduction was 96.7% and at 10.5-15.5 g ZnO NP of 19.8%. The metal reduction rate was R
    max
    for Fe 16.4; Zn 21.1; Pb 41.9; Hg 19.1 was higher than Ni 6.4 and Cu 11.3 from the FAS at 5.5 g ZnO NP whereas, the reduction rate of Pb showed highest. With doses of 5.5 g ZnO NP the biomass increased upto 78%; the metal reduced upto 98.7% with the share of 100% ZnO NP from FAS. Further investigation with phytotoxicity the plant reactive oxygen species (ROS) production were affected due was mainly due to the recovery of metals from FAS (R2 = 0.99).
  6. Iqbal MO, Naeem M, Mumtaz A, Ahmed MM, Ahmad A, Riaz R, et al.
    Am J Transl Res, 2022;14(10):7178-7188.
    PMID: 36398251
    OBJECTIVE: Jatropha mollissima is one of the most valuable medicinal plants used for the treatment of hepatic disorders. It is evident that 500 mg/kg of sodium valproate causes the hepatotoxicity, ototoxicity, gastrotoxicity, bone marrow suppression, and inflammation. This study was designed to explore the medicinal uses of Jatropha mollissima in hepatic disorders.

    METHODS: Hepatotoxicity was induced in Wister albino rats by injecting sodium valproate at the rate of 500 mg/kg once daily for fourteen days. Six male rats, each weighing 220-270 g, were placed into four separate groups for the study. The first group was treated with normal saline. Treatment of the second group was carried out by SVP for four days consecutively together with saline for three weeks. Group three and four were treated with sodium valproate and Jm hydroalcoholic extract applied in the concentrations of the 200 mg/kg and 400 mg/kg for the period of the three weeks. Phytochemical screening and HPLC analysis were conducted to identify the phytochemical nature and polyphenols in extract, respectively. DPPH, SOD, and NO tests were performed to measure the antioxidant activity.

    RESULTS: With the initial dose of treatments to rats, anatomic, physiological, or histopathologic abnormalities were detected. After three weeks, extract of Jatropha mollissima was used to treat the valproic acid-induced hepatotoxicity (P < 0.05).

    CONCLUSION: It was concluded that sodium valproate (SVP) and Jm extract were administered together. The hepatoprotective effects were extraordinarily high, with high concentrations of 400 mg/kg.

  7. Wang PS, Ahmad A, Nazar M, Rahmah AU, Moniruzzaman M
    Molecules, 2023 Aug 01;28(15).
    PMID: 37570764 DOI: 10.3390/molecules28155794
    Oil spill remediation plays a vital role in mitigating the environmental impacts caused by oil spills. The chemical method is one of the widely recognized approaches in chemical surfactants. However, the most commonly used chemical surfactants are toxic and non-biodegradable. Herein, two biocompatible and biodegradable surfactants were synthesized from orange peel using the ionic liquid 1-butyl-3-methylimidazolium chloride (BMIMCl) and organic solvent dimethylacetamide (CH3CN(CH3)2) as reaction media. The acronyms SOPIL and SOPOS refer to the surfactants prepared with BMIMCl and dimethylacetamide, respectively. The surface tension, dispersant effectiveness, optical microscopy, and emulsion stability test were conducted to examine the comparative performance of the synthesized surfactants. The Baffled flask test (BFT) was carried out to determine the dispersion effectiveness. The toxicity test was performed against zebrafish (Danio rerio), whereas the closed bottle test (CBT) evaluated biodegradability. The results revealed that the critical micelle concentration (CMC) value of SOPIL was lower (8.57 mg/L) than that of SOPOS (9.42 mg/L). The dispersion effectiveness values for SOPIL and SOPOS were 69.78% and 40.30%, respectively. The acute toxicity test demonstrated that SOPIL was 'practically non-toxic' with a median lethal concentration of more than 1000 mg/L after 96 h. The biodegradation rate was recorded as higher than 60% for both surfactants within 28 days, demonstrating their readily biodegradable nature. Considering these attributes, biocompatible and biodegradable surfactants derived from orange peel emerge as a promising and sustainable alternative for oil spill remediation.
  8. Um Min Allah N, Berahim Z, Ahmad A, Kannan TP
    Tissue Eng Regen Med, 2017 Oct;14(5):495-505.
    PMID: 30603504 DOI: 10.1007/s13770-017-0065-y
    Advancement in cell culture protocols, multidisciplinary research approach, and the need of clinical implication to reconstruct damaged or diseased tissues has led to the establishment of three-dimensional (3D) test systems for regeneration and repair. Regenerative therapies, including dental tissue engineering, have been pursued as a new prospect to repair and rebuild the diseased/lost oral tissues. Interactions between the different cell types, growth factors, and extracellular matrix components involved in angiogenesis are vital in the mechanisms of new vessel formation for tissue regeneration. In vitro pre-vascularization is one of the leading scopes in the tissue-engineering field. Vascularization strategies that are associated with co-culture systems have proved that there is communication between different cell types with mutual beneficial effects in vascularization and tissue regeneration in two-dimensional or 3D cultures. Endothelial cells with different cell populations, including osteoblasts, smooth muscle cells, and fibroblasts in a co-culture have shown their ability to advocate pre-vascularization. In this review, a co-culture perspective of human gingival fibroblasts and vascular endothelial cells is discussed with the main focus on vascularization and future perspective of this model in regeneration and repair.
  9. Magurran AE, Khachonpisitsak S, Ahmad AB
    J Fish Biol, 2011 Dec;79(6):1393-412.
    PMID: 22136233 DOI: 10.1111/j.1095-8649.2011.03091.x
    For over 150 years, ecologists have been striving to explain fundamental patterns of biological diversity, such as the observation that communities invariably consist of common and rare species, and to unravel the processes that underpin these patterns. This task is increasingly urgent given the accelerating loss of biological diversity. Although fishes are the most diverse vertebrate taxon and fish communities occur in a wide range of habitats, they have been relatively little studied in the quest to elucidate the processes that shape patterns of biological diversity. Here, some of the topics that investigations of fish assemblages can illuminate are highlighted. These include the characteristics of ecological communities and the role that dispersal limitation plays in structuring them, the distinction between core and occasional species, the insights that evaluating abundance in different currencies can bring and the assessment of community capacity. Questions are identified that future investigations of fish communities might tackle and a case study of a biodiverse ecoregion (Thailand and Peninsula Malaysia) is used to illustrate the need for better links between these ecological questions and effective conservation practice.
  10. Aleid GM, Alshammari AS, Alomari AD, Ahmad A, Alaysuy O, Ibrahim MNM
    PMID: 37632620 DOI: 10.1007/s11356-023-29430-8
    The benthic microbial fuel cell (BMFC) is one of the most efficient types of bioelectrochemical fuel cell systems. Modern bioelectrochemical fuel cells have several drawbacks, including an unstable organic substrate and a microorganism-unfriendly atmosphere. The recent literature to encounter such issues is one of the emerging talks. Researchers are focusing on the utilization of biomass and waste to encounter such challenges and make the technique more feasible at the pilot scale. This study investigated the combination of local bakery waste as an organic substrate with lignocellulosic biomass material. The whole experiment was conducted for 45 days. At an external resistance of 1000 ῼ and an internal resistance of 677 ῼ, the power density was found to be 3.51 mW/m2. Similarly, for Pb2+, Cd2+, Cr3+, Ni2+, and Co2+, the degradation efficiency was 84.40%, 81.21%, 80%, 89.50%, and 86.0%, respectively. The bacterial identification results showed that Liquorilactobacillus nagelii, Proteus mirabilis, Pectobacterium punjabense, and Xenorhabdus thuongxuanensis are the most prominent species found on anode biofilm. The method of electron generation in this study, which includes the degradation of metal ions, is also well described. Lastly, optimising the parameters showed that pH 7 provides a feasible environment for operation. A few future suggestions for practical steps are enclosed for the research community.
  11. Ali SG, Jalal M, Ahmad H, Umar K, Ahmad A, Alshammari MB, et al.
    Molecules, 2022 Dec 08;27(24).
    PMID: 36557818 DOI: 10.3390/molecules27248685
    Antimicrobial resistance has posed a serious health concern worldwide, which is mainly due to the excessive use of antibiotics. In this study, gold nanoparticles synthesized from the plant Tinospora cordifolia were used against multidrug-resistant Pseudomonas aeruginosa. The active components involved in the reduction and stabilization of gold nanoparticles were revealed by gas chromatography-mass spectrophotometry(GC-MS) of the stem extract of Tinospora cordifolia. Gold nanoparticles (TG-AuNPs) were effective against P. aeruginosa at different concentrations (50,100, and 150 µg/mL). TG-AuNPs effectively reduced the pyocyanin level by 63.1% in PAO1 and by 68.7% in clinical isolates at 150 µg/mL; similarly, swarming and swimming motilities decreased by 53.1% and 53.8% for PAO1 and 66.6% and 52.8% in clinical isolates, respectively. Biofilm production was also reduced, and at a maximum concentration of 150 µg/mL of TG-AuNPs a 59.09% reduction inPAO1 and 64.7% reduction in clinical isolates were observed. Lower concentrations of TG-AuNPs (100 and 50 µg/mL) also reduced the pyocyanin, biofilm, swarming, and swimming. Phenotypically, the downregulation of exopolysaccharide secretion from P. aeruginosa due to TG-AuNPs was observed on Congo red agar plates.
  12. Akbar MA, Mohd Yusof NY, Tahir NI, Ahmad A, Usup G, Sahrani FK, et al.
    Mar Drugs, 2020 Feb 05;18(2).
    PMID: 32033403 DOI: 10.3390/md18020103
    Saxitoxin is an alkaloid neurotoxin originally isolated from the clam Saxidomus giganteus in 1957. This group of neurotoxins is produced by several species of freshwater cyanobacteria and marine dinoflagellates. The saxitoxin biosynthesis pathway was described for the first time in the 1980s and, since then, it was studied in more than seven cyanobacterial genera, comprising 26 genes that form a cluster ranging from 25.7 kb to 35 kb in sequence length. Due to the complexity of the genomic landscape, saxitoxin biosynthesis in dinoflagellates remains unknown. In order to reveal and understand the dynamics of the activity in such impressive unicellular organisms with a complex genome, a strategy that can carefully engage them in a systems view is necessary. Advances in omics technology (the collective tools of biological sciences) facilitated high-throughput studies of the genome, transcriptome, proteome, and metabolome of dinoflagellates. The omics approach was utilized to address saxitoxin-producing dinoflagellates in response to environmental stresses to improve understanding of dinoflagellates gene-environment interactions. Therefore, in this review, the progress in understanding dinoflagellate saxitoxin biosynthesis using an omics approach is emphasized. Further potential applications of metabolomics and genomics to unravel novel insights into saxitoxin biosynthesis in dinoflagellates are also reviewed.
  13. Ahmad A, Zulaily N, Abdul Manan NS, Shahril MR, Syed Saadun Tarek Wafa SW, Mohd Amin R, et al.
    BMC Public Health, 2017 01 05;17(1):9.
    PMID: 28056904 DOI: 10.1186/s12889-016-3911-2
    BACKGROUND: Body weight is highly associated with overall health status. Being severely thin or obese may impose the risk of many health problems. Early detection of body mass index (BMI) status may help to reduce the associated comorbidities. Although many studies in the literature have investigated the BMI of school adolescents in Malaysia, the data on status of body weight among school adolescents in suburban states like Terengganu is limited. This study aimed to describe the body weight status of the whole population of school adolescents in all seven districts in Terengganu, Malaysia.

    METHODS: Using a cross-sectional study design, body weight and height were measured, and BMI was calculated and classified using WHO BMI-for-age Z-score. Data was obtained using the National Fitness Standard (SEGAK) assessment, which was uploaded in a specific Health Monitoring System (HEMS).

    RESULTS: From a total of 62,567 school adolescents, 50.7% were boys and 49.3% were girls. Girls had significantly higher BMI than boys in age groups of 13 to 15 and 16 to 17 years old. Among boys and girls, there were significant differences in mean BMI of school adolescents between rural and urban school locations in all age groups (p 

  14. Samsuri NM, Zamzuri AK, Al-Mansoori MH, Ahmad A, Mahdi MA
    Opt Express, 2008 Oct 13;16(21):16475-80.
    PMID: 18852754
    We demonstrate an enhanced architecture of Brillouin-Erbium fiber laser utilizing the reverse-S-shaped fiber section as the coupling mechanism. The enhancement is made by locating a common section of Erbium-doped fiber next to the single-mode fiber to amplify the Brillouin pumps and the oscillating Stokes lines. The requirement of having two Erbium gain sections to enhance the multiple Brillouin Stokes lines generation is neglected by the proposed fiber laser structure. The mode competitions arise from the self-lasing cavity modes of the fiber laser are efficiently suppressed by the stronger pre-amplified Brillouin pump power before entering the single mode fiber section. The maximum output power of 20 mW is obtained from the proposed fiber laser with 10 laser lines that equally separated by 0.089 nm spacing.
  15. Zamzuri AK, Md Ali MI, Ahmad A, Mohamad R, Mahdi MA
    Opt Lett, 2006 Apr 01;31(7):918-20.
    PMID: 16599211
    We demonstrate a multiple-wavelength Brillouin comb laser with cooperative Rayleigh scattering that uses Raman amplification in dispersion-compensating fiber. The laser resonator is a linear cavity formed by reflector at each end of the dispersion-compensating fiber to improve the reflectivity of the Brillouin Stokes comb. Multiple Brillouin Stokes generation has been improved in terms of optical signal-to-noise ratio and power-level fluctuation between neighboring channels. Furthermore, the linewidth of the Brillouin Stokes is uniform within the laser output bandwidth.
  16. Ahmad A, Nazar Z, Swaminathan D
    Oral Health Prev Dent, 2018 7 22;16(3):281-289.
    PMID: 30027167 DOI: 10.3290/j.ohpd.a40759
    PURPOSE: To investigate the association between plasma CRP levels and periodontal disease during pregnancy.

    MATERIALS AND METHODS: Fifty-six pregnant women attending the Antenatal Clinic, UMMC for their first antenatal check-up consented and were recruited for this study: 28 subjects with diseased periodontium (test group) and 28 subjects with healthy periodontium (control). The test group underwent nonsurgical periodontal therapy and the control group was given oral hygiene education. Periodontal parameters and CRP levels were evaluated at baseline and 6 weeks. Pregnancy outcome data were recorded from the Antenatal Clinic, UMMC.

    RESULTS: Plasma CRP levels in the test group were statistically significantly elevated compared to the control group (8.55 ± 5.28 mg/l vs 5.66 ± 2.91 mg/l). After nonsurgical periodontal therapy, a statistically significant reduction in the CRP level in the test group (2.06 mg/l) along with statistically significant improvement in periodontal status in both groups was observed. The mean birth weight for infants of both groups showed no statistically significant difference.

    CONCLUSIONS: Plasma CRP levels in pregnant women with diseased periodontium were statistically significantly reduced after nonsurgical periodontal therapy. However, no association between CRP levels and adverse pregnancy outcome was observed.

  17. Tumasyan A, Adam W, Andrejkovic JW, Bergauer T, Chatterjee S, Damanakis K, et al.
    Eur Phys J C Part Fields, 2023;83(7):587.
    PMID: 37440247 DOI: 10.1140/epjc/s10052-023-11630-8
    New sets of parameter tunes for two of the colour reconnection models, quantum chromodynamics-inspired and gluon-move, implemented in the pythia  8 event generator, are obtained based on the default CMS pythia  8 underlying-event tune, CP5. Measurements sensitive to the underlying event performed by the CMS experiment at centre-of-mass energies s=7 and 13TeV, and by the CDF experiment at 1.96TeV are used to constrain the parameters of colour reconnection models and multiple-parton interactions simultaneously. The new colour reconnection tunes are compared with various measurements at 1.96, 7, 8, and 13TeV including measurements of the underlying-event, strange-particle multiplicities, jet substructure observables, jet shapes, and colour flow in top quark pair (tt¯) events. The new tunes are also used to estimate the uncertainty related to colour reconnection modelling in the top quark mass measurement using the decay products of tt¯ events in the semileptonic channel at 13TeV.
  18. Mohamad NA, Nasef MM, Abdullah TAT, Ahmad A, Ting TM
    Environ Sci Pollut Res Int, 2023 Nov;30(55):116906-116920.
    PMID: 37121947 DOI: 10.1007/s11356-023-26913-6
    A series of fibrous aminated adsorbents for CO2 adsorption were prepared by covalent incorporation of poly (glycidyl methacrylate) (PGMA) by graft copolymerization of GMA onto electron beam (EB) irradiated polyethylenepolypropylene (PE/PP) fibrous sheets and subsequent amination with ethylenediamine (EDA), diethylenetriamine (DETA), or tetraethylenepentamine (TEPA). The physico-chemical properties of the adsorbents were evaluated using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA), X-ray diffraction (XRD), and Brunauer-Emmett-Teller (BET) analysis. All the adsorbents displayed typic primary and secondary amine features combined with a decrease in both of crystallinity and surface area of PE/PP, and such a decrease was higher in adsorbents with longer aliphatic chain of the amine. Of all adsorbents, TEPA-containing fibres showed the highest CO2 adsorption capacity and thus was further investigated for CO2 capture from CO2/CH4 mixtures of different gas ratios under various pressures and temperatures. The selectivity of CO2 over CH4 and equilibrium isotherms, kinetics, and thermodynamics of the adsorption on the fibrous aminated adsorbent were all investigated. The Sips model was found to best fit the isotherm of CO2 adsorption suggesting the presence of a combination of monolayer and multilayer adsorptions. The adsorption kinetic data was found to best fit Elovich model reflecting chemisorption. The ΔG°, ΔS°, and ΔH° showed positive values suggesting that the adsorption of CO2 on the present fibrous adsorbent was non-spontaneous with an increase in randomness implying that the process was endothermic. Overall, it can be suggested that PE/PP-g-PGMA/TEPA adsorbent has a strong potential for separation of CO2 from NG.
  19. Ahmad A, Ahmad AH, Dieng H, Satho T, Ahmad H, Aziz AT, et al.
    J Med Entomol, 2011 Nov;48(6):1236-46.
    PMID: 22238885
    There is accumulating evidence that criminals wrap dead bodies in an attempt to conceal evidence. To anticipate the forensic implications of this phenomenon, we examined whether flies that are naturally associated with cadavers exhibit a delay in attendance or differ in species composition and abundance patterns because of the presence of wrapping material. Wrapped and exposed carcasses of dead monkeys placed in an oil plantation in Kedah, Malaysia, were visited over 50 d. On daily visits to each of the six carcasses, visiting adult flies were sampled using hand nets. Flies of 12 families were encountered. Calliphoridae (Chrysomya rufifacies Macquart and C. megacephala (F.) was the most prevalent family, followed by Sphaeroceridae. Some families tended to be more abundant in WRCs (i.e., Calliphoridae, Muscidae, and Phoridae), whereas others (i.e., Piophilidae, Sepsidae, and Psychodidae) were more prevalent in exposed carcasses. Wrapping delayed the arrival of all fly species encountered, with delays varying from 1 to 13 d depending on species. Wrapping did not affect species composition of flies, but prolong the occurrence of some species. The results of the current study emphasize the need to take into consideration the presence of a wrap when estimating postmortem interval.
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