MyMedR

Displaying publications 1081 - 1100 of 1777 in total

Abstract:

Sort:

Development and application of dot-enzyme-linked immunosorbent (dot-ELISA) assay for detection of Brucella melitensis and evaluation of the shedding pattern in infected goats

Onilude OM, Mohd Yusoff S, Emikpe BO, Tanko P, Shahrom SM, Effendy M

J Immunoassay Immunochem, 2017;38(1):82-99.
PMID: 27494045 DOI: 10.1080/15321819.2016.1220395

Early and accurate diagnosis of Brucella melitensis is essential for the treatment and control of brucellosis both in animals and humans. The thrust for the development of a rapid diagnostic technique to overcome the limitations of conventional microbiological and serological tests brought about this investigation on the development and application of dot-ELISA for antigen and antibody detection in infected goats. Fifteen apparently healthy Boer aged 2-3 years which tested negative for brucellosis using PCR and ELISA, were grouped into A (10 goats infected intraocularly with 10(7) CFU of B. melitensis) and B (5 goats) as control. Discharges (ocular, nasal, and vaginal) and blood were collected at days 3, 7, 10, 14, weekly until 42 post-infection (pi) for dot-ELISA, PCR, and RBPT. Dot-ELISA detected B. melitensis antigen and antibody in group A at day 3 and 7 pi, respectively with adequate sensitivity and specificity relative to PCR and RBPT. The bacteria shedding detected from discharges at day 3 pi in the nasal and ocular route with dot-ELISA. Group B were consistently negative. Values such as speed, simplicity, field adaptability, high sensitivity, and specificity make dot-ELISA a rapid and adequate technique for diagnosis of brucellosis in B. melitensis infected goats within few hours.
Improved collagen extraction from jellyfish (Acromitus hardenbergi) with increased physical-induced solubilization processes

Khong NMH, Yusoff FM, Jamilah B, Basri M, Maznah I, Chan KW, et al.

Food Chem, 2018 Jun 15;251:41-50.
PMID: 29426422 DOI: 10.1016/j.foodchem.2017.12.083

Efficiency and effectiveness of collagen extraction process contribute to huge impacts to the quality, supply and cost of the collagen produced. Jellyfish is a potential sustainable source of collagen where their applications are not limited by religious constraints and threats of transmittable diseases. The present study compared the extraction yield, physico-chemical properties and toxicology in vitro of collagens obtained by the conventional acid-assisted and pepsin-assisted extraction to an improved physical-aided extraction process. By increasing physical intervention, the production yield increased significantly compared to the conventional extraction processes (p  .05) while retaining high molecular weight distributions and polypeptide profiles similar to those extracted using only acid. Moreover, they exhibited better appearance, instrumental colour and were found to be non-toxic in vitro and free of heavy metal contamination.
Fulltext A 52-month follow-up on the transvaginal mesh surgery in vaginal cuff eversion

Lo TS, Yusoff FM, Kao CC, Jaili S, Uy Patrimonio MC

Taiwan J Obstet Gynecol, 2017 Jun;56(3):346-352.
PMID: 28600046 DOI: 10.1016/j.tjog.2017.04.015

OBJECTIVE: Transvaginal mesh anterior-posterior (TVM-AP) provides better cure rates in the surgical treatment of vaginal cuff eversion than anterior transvaginal mesh combined with sacrospinous ligament fixation (TVM-A). We determine the outcomes after TVM-A and TVM-AP surgeries in advanced vaginal cuff prolapse.
MATERIALS AND METHODS: The charts of 796 women who underwent pelvic organ prolapse (POP) surgery from July 2006 to January 2012 in Chang Gung Memorial Hospitals were reviewed. We included women who presented with advanced cuff eversion and treated with TVM surgery. Data were analysed after three years post-surgery. Descriptive statistics were used for demographic and perioperative data. The paired-samples t test was used for comparison of preoperative and postoperative continuous data. The outcomes measured were objective cure (POP-Q stage ≤ 1) and subjective cure (negative response to question 2 and 3 on POPDI-6).
RESULTS: A total of 97 patients was analysed. 61 patients had TVM-A and 36 patients had TVM-AP insertion. Mean follow-up was 52 months. The objective cure rate for TVM-AP was significantly higher than TVM-A, 94.4% versus 80.3%. TVM-AP also showed a higher subjective cure rate (91.7%) though there was no significant difference from TVM-A (p = 0.260). The mesh extrusion rate was low at 3.1% with no major complications seen. In TVM-A the blood loss was lesser and the operation time was shorter.
CONCLUSION: TVM-AP showed better objective cure rate than TVM-A at 52 months. However, TVM-A is less invasive in comparison with an acceptably good cure rates.
Fulltext Corrigendum to "Safety and Clinical Usage of Newcastle Disease Virus in Cancer Therapy"

Lam HY, Yeap SK, Pirozyan MR, Omar AR, Yusoff K, Abd-Aziz S, et al.

Biomed Res Int, 2017;2017:4529437.
PMID: 29333441 DOI: 10.1155/2017/4529437

[This corrects the article DOI: 10.1155/2011/718710.].
Successful sperm cryopreservation of the brown-marbled grouper, Epinephelus fuscoguttatus using propylene glycol as cryoprotectant

Yusoff M, Hassan BN, Ikhwanuddin M, Sheriff SM, Hashim F, Mustafa S, et al.

Cryobiology, 2018 04;81:168-173.
PMID: 29355519 DOI: 10.1016/j.cryobiol.2018.01.005

This study developed the cryopreservation of brown-marbled grouper spermatozoa for practical application. We examined 32 cryodiluents, developed from four types of cryoprotectants [propylene glycol (PG), dimethyl-sulphoxide (Me2SO), dimethyl-acetamide (DMA) and ethylene glycol (EG)] at four concentrations of 5, 10, 15 and 20% in combination with two extenders [Fetal bovine serum (FBS) and artificial seminal plasma (ASP). Cooling rates were examined by adjusting the height of straws (2.5-12.5 cm) from the liquid nitrogen (LN) vapor and cooled for 5 min before immersion into LN. DNA laddering was used to detect DNA damage in cryopreserved sperm. In fertilization trials, 0.5 g of eggs was mixed with cryopreserved sperm stored for 30 days in LN. The best motility of post-thaw sperm was achieved using 15% PG + 85% FBS (76.7 ± 8.8%); 10% PG + 90% FBS was also effective as cryodiluent. Generally, FBS gave better post-thaw motility compared to ASP. The optimum cooling rate was at 17.6 °C min-1 obtained by freezing at the height of 7.5 cm surface of LN. The results obtained showed that cryopreserved sperm of brown-marbled grouper suffered slight DNA fragmentation, which resulted in significantly lower motility. However, the fertilization (90.9 ± 0.5%), hatching (64.5 ± 4.1%) and deformity rates (3.8 ± 0.2%) obtained from cryopreserved sperm showed no significant difference with fresh sperm. These findings show that the developed protocol for cryopreservation of brown-marbled grouper sperm was viable and will be useful for successful breeding and seed production of brown-marbled grouper.
Floc behavior and removal mechanisms of cross-linked Durio zibethinus seed starch as a natural flocculant for landfill leachate coagulation-flocculation treatment

Yusoff MS, Aziz HA, Zamri MFMA, Suja' F, Abdullah AZ, Basri NEA

Waste Manag, 2018 Apr;74:362-372.
PMID: 29370968 DOI: 10.1016/j.wasman.2018.01.016

This study investigated the behavior and mechanisms of cross-linked Durio zibethinus seed starch (CDSS) flocculants for landfill leachate treatment. A physical-chemical treatment method of coagulation-flocculation process and starch modification were implemented in treating stabilized leachate from Matang Landfill, Perak, Malaysia. In practical, the removal performance of color, COD, suspended solid and turbidity for CDSS flocculants were evaluated by combining with primary coagulant of polyaluminium chloride (PAC). In this study, the application of crosslinking modification for Durio zibethinus seed waste starch flocculants showed good improvement. The impurities removal for colour, COD, suspended solid and turbidity were increased by the addition of CDSS flocculants. Furthermore, the average size of the floc was also increased from 60.24 µm to 89.5 µm. Despite, the addition of CDSS flocculants produced a reduction of PAC coagulant from 2700 mg/L to 2200 mg/L, with 500 mg/L reduction on the PAC dosage dependency. Therefore, these results affirmed the potentials of crosslinked modification for Durio zibethinus seed waste starch flocculants in landfill leachate treatment.
Environment-Modulated Crystallization of Cu2O and CuO Nanowires by Electrospinning and Their Charge Storage Properties

Harilal M, G Krishnan S, Pal B, Reddy MV, Ab Rahim MH, Yusoff MM, et al.

Langmuir, 2018 02 06;34(5):1873-1882.
PMID: 29345940 DOI: 10.1021/acs.langmuir.7b03576

This article reports the synthesis of cuprous oxide (Cu2O) and cupric oxide (CuO) nanowires by controlling the calcination environment of electrospun polymeric nanowires and their charge storage properties. The Cu2O nanowires showed higher surface area (86 m2 g-1) and pore size than the CuO nanowires (36 m2 g-1). Electrochemical analysis was carried out in 6 M KOH, and both the electrodes showed battery-type charge storage mechanism. The electrospun Cu2O electrodes delivered high discharge capacity (126 mA h g-1) than CuO (72 mA h g-1) at a current density of 2.4 mA cm-2. Electrochemical impedance spectroscopy measurements show almost similar charge-transfer resistance in Cu2O (1.2 Ω) and CuO (1.6 Ω); however, Cu2O showed an order of magnitude higher ion diffusion. The difference in charge storage between these electrodes is attributed to the difference in surface properties and charge kinetics at the electrode. The electrode also shows superior cyclic stability (98%) and Coulombic efficiency (98%) after 5000 cycles. Therefore, these materials could be acceptable choices as a battery-type or pseudocapacitive electrode in asymmetric supercapacitors.
A heat capacity model of T3/2 dependence for quantum dots

Roslee AE, Muzakir SK, Ismail J, Yusoff MM, Jose R

Phys Chem Chem Phys, 2016 Dec 21;19(1):408-418.
PMID: 27905607 DOI: 10.1039/c6cp07173b

This article addresses the heat capacity of quantum dots (QDs) using density functional theory (DFT). By analyzing the evolution of phonon density of states and heat capacity as CdSe clusters grow from a molecular cluster into larger quantum confined solids, we have shown that their heat capacity does not fit very well with the Debye T3 model. We observed that the number of phonon modes, which is discrete, increases as the particles grow, and the dispersion relation shows a quadratic behavior in contrast to the bulk solids whose dispersion relation is linear and equal to the sound velocity. The phonon density of states showed a square root variation with respect to frequency whereas that of the bulk is a quadratic variation of frequency. From the observed variation in the phonon density of states and holding the fact that the atomic vibrations in solids are elastically coupled, we have re-derived the expression for total energy of the QDs and arrive at a T3/2 model of heat capacity, which fits very well to the observed heat capacity data. These results give promising directions in the understanding of the evolution of the thermophysical properties of solids.
Bio-waste corn-cob cellulose supported poly(hydroxamic acid) copper complex for Huisgen reaction: Waste to wealth approach

Mandal BH, Rahman ML, Yusoff MM, Chong KF, Sarkar SM

Carbohydr Polym, 2017 Jan 20;156:175-181.
PMID: 27842811 DOI: 10.1016/j.carbpol.2016.09.021

Corn-cob cellulose supported poly(hydroxamic acid) Cu(II) complex was prepared by the surface modification of waste corn-cob cellulose through graft copolymerization and subsequent hydroximation. The complex was characterized by IR, UV, FESEM, TEM, XPS, EDX and ICP-AES analyses. The complex has been found to be an efficient catalyst for 1,3-dipolar Huisgen cycloaddition (CuAAC) of aryl/alkyl azides with a variety of alkynes as well as one-pot three-components reaction in the presence of sodium ascorbate to give the corresponding cycloaddition products in up to 96% yield and high turn over number (TON 18,600) and turn over frequency (TOF 930h-1) were achieved. The complex was easy to recover from the reaction mixture and reused six times without significant loss of its catalytic activity.
One-Dimensional Assembly of Conductive and Capacitive Metal Oxide Electrodes for High-Performance Asymmetric Supercapacitors

Harilal M, Vidyadharan B, Misnon II, Anilkumar GM, Lowe A, Ismail J, et al.

ACS Appl Mater Interfaces, 2017 Mar 29;9(12):10730-10742.
PMID: 28266837 DOI: 10.1021/acsami.7b00676

A one-dimensional morphology comprising nanograins of two metal oxides, one with higher electrical conductivity (CuO) and the other with higher charge storability (Co3O4), is developed by electrospinning technique. The CuO-Co3O4 nanocomposite nanowires thus formed show high specific capacitance, high rate capability, and high cycling stability compared to their single-component nanowire counterparts when used as a supercapacitor electrode. Practical symmetric (SSCs) and asymmetric (ASCs) supercapacitors are fabricated using commercial activated carbon, CuO, Co3O4, and CuO-Co3O4 composite nanowires, and their properties are compared. A high energy density of ∼44 Wh kg-1 at a power density of 14 kW kg-1 is achieved in CuO-Co3O4 ASCs employing aqueous alkaline electrolytes, enabling them to store high energy at a faster rate. The current methodology of hybrid nanowires of various functional materials could be applied to extend the performance limit of diverse electrical and electrochemical devices.
Production of oil palm (Elaeis guineensis) fronds lignin-derived non-toxic aldehyde for eco-friendly wood adhesive

Hazwan Hussin M, Samad NA, Latif NHA, Rozuli NA, Yusoff SB, Gambier F, et al.

Int J Biol Macromol, 2018 Jul 01;113:1266-1272.
PMID: 29548919 DOI: 10.1016/j.ijbiomac.2018.03.048

Lignocellulosic materials can significantly contribute to the development of eco-friendly wood adhesives. In this work, glyoxal-phenolic resins for plywood were prepared using organosolv lignin, which was isolated from black liquor recovered from organosolv pulping of oil palm fronds (OPF) and considered to be an alternative to phenol. Glyoxal, which is a dialdehyde obtained from several natural resources, was used as substitute for formaldehyde. The structure of organosolv lignin and the resins were characterized by FTIR and NMR, and for thermal stability by TGA and DSC. The resins were further studied for their viscosity, pH, solids content and gel times. The resins performance as wood adhesive was further established from mechanical test in terms of tensile strength and modulus of elasticity (MOE) to obtain the optimum ratios of organosolv lignin, which replaces phenol in organosolv lignin phenol glyoxal (OLPG) resins. The adhesive composition having 50% (w/w) of phenol substituted by organosolv lignin, termed as 50% OLPG showed highest adhesive strength compared to phenol formaldehyde (PF) commercial adhesive.
Fulltext Prediction of Strength Properties of Concrete Containing Waste Marble Aggregate and Stone Dust-Modeling and Optimization Using RSM

Zamir Hashmi SR, Khan MI, Khahro SH, Zaid O, Shahid Siddique M, Md Yusoff NI

Materials (Basel), 2022 Nov 14;15(22).
PMID: 36431509 DOI: 10.3390/ma15228024

Carbon footprint reduction, recompense depletion of natural resources, as well as waste recycling are nowadays focused research directions to achieve sustainability without compromising the concrete strength parameters. Therefore, the purpose of the present study is to utilize different dosages of marble waste aggregates (MWA) and stone dust (SD) as a replacement for coarse and fine aggregate, respectively. The MWA with 10 to 30% coarse aggregate replacement and SD with 40 to 50% fine aggregate replacement were used to evaluate the physical properties (workability and absorption), durability (acid attack resistance), and strength properties (compressive, flexural, and tensile strength) of concrete. Moreover, statistical modeling was also performed using response surface methodology (RSM) to design the experiment, optimize the MWA and SD dosages, and finally validate the experimental results. Increasing MWA substitutions resulted in higher workability, lower absorption, and lower resistance to acid attack as compared with controlled concrete. However, reduced compressive strength, flexural strength, and tensile strength at 7-day and 28-day cured specimens were observed as compared to the controlled specimen. On the other hand, increasing SD content causes a reduction in workability, higher absorption, and lower resistance to acid attack compared with controlled concrete. Similarly, 7-day and 28-day compressive strength, flexural strength, and tensile strength of SD-substituted concrete showed improvement up to 50% replacement and a slight reduction at 60% replacement. However, the strength of SD substituted concrete is higher than controlled concrete. Quadratic models were suggested based on a higher coefficient of determination (R2) for all responses. Quadratic RSM models yielded R2 equaling 0.90 and 0.94 for compressive strength at 7 days and 28 days, respectively. Similarly, 0.94 and 0.96 for 7-day and 28-day flexural strength and 0.89 for tensile strength. The optimization performed through RSM indicates that 15% MWA and 50% SD yielded higher strength compared to all other mixtures. The predicted optimized data was validated experimentally with an error of less than 5%.
Fulltext Lactococcus lactis secreting phage lysins as a potential antimicrobial against multi-drug resistant Staphylococcus aureus

Chandran C, Tham HY, Abdul Rahim R, Lim SHE, Yusoff K, Song AA

PeerJ, 2022;10:e12648.
PMID: 35251775 DOI: 10.7717/peerj.12648

BACKGROUND: Staphylococcus aureus is an opportunistic Gram-positive bacterium that can form biofilm and become resistant to many types of antibiotics. The treatment of multi-drug resistant Staphylococcus aureus (MDRSA) infection is difficult since it possesses multiple antibiotic-resistant mechanisms. Endolysin and virion-associated peptidoglycan hydrolases (VAPGH) enzymes from bacteriophage have been identified as potential alternative antimicrobial agents. This study aimed to assess the ability of Lactococcus lactis NZ9000 secreting endolysin and VAPGH from S. aureus bacteriophage 88 to inhibit the growth of S. aureus PS 88, a MDRSA.
METHOD: Endolysin and VAPGH genes were cloned and expressed in L. lactis NZ9000 after fusion with the SPK1 signal peptide for secretion. The recombinant proteins were expressed and purified, then analyzed for antimicrobial activity using plate assay and turbidity reduction assay. In addition, the spent media of the recombinant lactococcal culture was analyzed for its ability to inhibit the growth of the S. aureus PS 88.
RESULTS: Extracellular recombinant endolysin (Endo88) and VAPGH (VAH88) was successfully expressed and secreted from L. lactis which was able to inhibit S. aureus PS 88, as shown by halozone formation on plate assays as well as inhibition of growth in the turbidity reduction assay. Moreover, it was observed that the spent media from L. lactis NZ9000 expressing Endo88 and VAH88 reduced the viability of PS 88 by up to 3.5-log reduction with Endo88 being more efficacious than VAH88. In addition, Endo88 was able to lyse all MRSA strains tested and Staphylococcus epidermidis but not the other bacteria while VAH88 could only lyse S. aureus PS 88.
CONCLUSION: Recombinant L. lactisNZ9000 expressing phage 88 endolysin may be potentially developed into a new antimicrobial agent for the treatment of MDRSA infection.
Fulltext In Silico Study and Effects of BDMC33 on TNBS-Induced BMP Gene Expressions in Zebrafish Gut Inflammation-Associated Arthritis

Mostofa F, Yasid NA, Shamsi S, Ahmad SA, Mohd-Yusoff NF, Abas F, et al.

Molecules, 2022 Nov 28;27(23).
PMID: 36500396 DOI: 10.3390/molecules27238304

The bone morphogenic protein (BMP) family is a member of the TGF-beta superfamily and plays a crucial role during the onset of gut inflammation and arthritis diseases. Recent studies have reported a connection with the gut-joint axis; however, the genetic players are still less explored. Meanwhile, BDMC33 is a newly synthesized anti-inflammatory drug candidate. Therefore, in our present study, we analysed the genome-wide features of the BMP family as well as the role of BMP members in gut-associated arthritis in an inflammatory state and the ability of BDMC33 to attenuate this inflammation. Firstly, genome-wide analyses were performed on the BMP family in the zebrafish genome, employing several in silico techniques. Afterwards, the effects of curcumin analogues on BMP gene expression in zebrafish larvae induced with TNBS (0.78 mg/mL) were determined using real time-qPCR. A total of 38 identified BMP proteins were revealed to be clustered in five major clades and contain TGF beta and TGF beta pro peptide domains. Furthermore, BDMC33 suppressed the expression of four selected BMP genes in the TNBS-induced larvae, where the highest gene suppression was in the BMP2a gene (an eight-fold decrement), followed by BMP7b (four-fold decrement), BMP4 (four-fold decrement), and BMP6 (three-fold decrement). Therefore, this study reveals the role of BMPs in gut-associated arthritis and proves the ability of BDMC33 to act as a potential anti-inflammatory drug for suppressing TNBS-induced BMP genes in zebrafish larvae.
Fulltext The Needs of Orthopaedic Patients in Discharge Planning

Muhamad H, Yusoff M, Shokri AA, Sulaiman Z, Bakar RS, Zain NM

Malays Orthop J, 2022 Nov;16(3):36-43.
PMID: 36589375 DOI: 10.5704/MOJ.2211.007

INTRODUCTION: Patients' transition from hospital to home could be challenging for patients and caregivers. This is of utmost importance for patients requiring special or long-term care such as post-orthopaedic surgery. Effective discharge planning is required to ensure that patients are prepared to and get continuous care after returning home to prevent complications. Patients' need assessment is essential to develop effective discharge planning to meet the patient's needs.
MATERIALS AND METHODS: This mixed-method study aimed to determine the patient's needs to develop a discharge planning for total knee replacement surgery. The needs for 96 total knee replacement patients were assessed using the Needs Evaluation Questionnaire (NEQ). The in-depth interview primary focus was to explore the lived experience of the post-total knee replacement patients receiving care in the hospital.
RESULTS: A total of 96 participants (100%) completed the NEQ questionnaire. Most of the needs concerned by the participants were expressed by at least 70% of them except the financial need (59.4%). The semi-structured interview found two elements which were a support group and patients' needs in terms of emotional, physical and spiritual preparation in developing effective discharge planning.
CONCLUSION: This study clarified that the patient needs assessment in the patient care plan.
Fulltext Pharmacological activities and mechanisms of action of hypophyllanthin: A review

Wan Saidin WA, Jantan I, Abdul Wahab SM, Jalil J, Mohd Said M, Yusoff SD, et al.

Front Pharmacol, 2022;13:1070557.
PMID: 36699081 DOI: 10.3389/fphar.2022.1070557

Hypophyllanthin is a major lignan present in various Phyllanthus species and has been used as one of the bioactive chemical markers for quality control purposes as it contributes to their diverse pharmacological activities. The objective of this study is to compile up-to-date data on the pharmacological actions and mechanisms of hypophyllanthin. This review also includes the extracts of Phyllanthus species whose pharmacological actions have been partially attributed to hypophyllanthin. The scientific findings on the compound are critically analyzed and its potential as a lead molecule for the discovery of drug candidates for the development of therapeutics to treat diverse diseases is highlighted. Data collection was mainly through the exploration of Ovid-MEDLINE, Scopus, Science Direct, and Elsevier databases. Studies conducted in vitro and in vivo showed that hypophyllanthin had potent immunomodulating properties as well as a variety of other pharmacological properties, including anti-inflammatory, hepatoprotective, anti-tumor, anti-allergic, anti-hypertensive, and phytoestrogenic properties. Several mechanisms of action on the effects of hypophyllanthin on the immune system, in cancer and other disease states, were presented to provide some insights into its pharmacological effects. Before being submitted to clinical investigations, additional animal studies utilising different animal models are necessary to analyse its bioavailability, pharmacokinetics, and pharmacodynamic properties, as well as its toxicity, to determine its efficacy and safety. Understanding its potential as a lead molecule for the discovery of therapeutic candidates, particularly for the development of therapies for inflammatory and immune-related disorders, requires an understanding of its pharmacological activities and mechanisms of action. An insight into its pharmacological activities and mechanisms of action will provide an understanding of its potential as a lead compound for the discovery of drug candidates, especially for the development of therapies for inflammatory and immune related diseases.
Fulltext The usability evaluation on mobile learning apps with gamification for middle-aged women

Mohtar S, Jomhari N, Omar NA, Mustafa MBP, Yusoff ZM

Educ Inf Technol (Dordr), 2023;28(1):1189-1210.
PMID: 35912038 DOI: 10.1007/s10639-022-11232-z

Mobile learning and gamification are becoming increasingly popular, especially in education. Numerous researches suggest that mobile learning apps with gamification can improve student learning. However, there are fewer studies that analyze the gamification effects and usability of mobile apps for middle-aged women. The aim of this study is to propose a new mobile learning app with gamification aspects for the use of middle-aged women in memorizing Arabic words. In the preliminary study, pre and post-tests were conducted to determine the effect of the use of the gamification application on 61 middle-aged women using Kahoot! game. The results from the Kahoot! game shows that participants enjoy learning and can memorize Arabic words effectively, indicating that gamification can motivate learning among middle-aged women. Then the usability of a new mobile app prototype containing learning notes and quizzes for user self-learning purposes was evaluated using three (3) criteria namely effectiveness, efficiency, and satisfaction. The considered indicators to measure the application's effectiveness are (a) tapping behaviour (number of taps used to perform a specific task), (b) number of attempts (how many times users tried to complete the task successfully), and (c) quiz score (score obtained by user after self-learning using the mobile app). Two variables were used to measure the efficiency of the application: the duration of task completion and the number of requests for help. In order to evaluate the satisfaction, a questionnaire was used to determine how respondents rated the mobile app's usability. The usability evaluation results show that the proposed mobile app was effective as users could utilize all the modules provided and successfully answer the quiz questions.
Fulltext Shoulder Agony: A Painful Glenoid Cyst

Md Yusoff BAH, Ramlan MKR, Mohd Don AF, Muhammed Ali Noor MI, Mohamad N

Cureus, 2023 Jan;15(1):e33726.
PMID: 36788828 DOI: 10.7759/cureus.33726

A ganglion cyst is a benign cystic lesion that can occur intraosseously. It is commonly a multiloculated lesion that consists of fibrous tissue with mucoid change and is usually located in the subchondral bone adjacent to the joint. Here, we present a rare case of a 33-year-old woman who presented to our orthopedic clinic with a five-year history of shoulder pain that gradually worsened. The pain was worst after any activity involving the right shoulder, and it also occurred at night and disturbed her sleep. Physical examination showed that the patient had limitations on the extreme range of movement. MRI revealed a subchondral bone cyst at the glenoid fossa. The ganglion cyst was subsequently curetted and packed with a bone graft from the iliac bone. Postoperatively, the pain resolved and the patient's range of movement improved. At the six-month follow-up, new bone formation over the void area caused by the cyst was observed on MRI.
Mesenchymal Stromal Cells-Derived Exosome and the Roles in the Treatment of Traumatic Brain Injury

Mot YY, Moses EJ, Mohd Yusoff N, Ling KH, Yong YK, Tan JJ

Cell Mol Neurobiol, 2023 Mar;43(2):469-489.
PMID: 35103872 DOI: 10.1007/s10571-022-01201-y

Traumatic brain injury (TBI) could result in life-long disabilities and death. Though the mechanical insult causes primary injury, the secondary injury due to dysregulated responses following neuronal apoptosis and inflammation is often the cause for more detrimental consequences. Mesenchymal stromal cell (MSC) has been extensively investigated as the emerging therapeutic for TBI, and the functional properties are chiefly attributed to their secretome, especially the exosomes. Delivering these nanosize exosomes have shown to ameliorate post-traumatic injury and restore brain functions. Recent technology advances also allow engineering MSC-derived exosomes to carry specific biomolecules of interest to augment their therapeutic outcome. In this review, we discuss the pathophysiology of TBI and summarize the recent progress in the applications of MSCs-derived exosomes, the roles and the signalling mechanisms underlying the protective effects in the treatment of the TBI.
Fulltext Accuracy Analysis of Deep Learning Methods in Breast Cancer Classification: A Structured Review

Yusoff M, Haryanto T, Suhartanto H, Mustafa WA, Zain JM, Kusmardi K

Diagnostics (Basel), 2023 Feb 11;13(4).
PMID: 36832171 DOI: 10.3390/diagnostics13040683

Breast cancer is diagnosed using histopathological imaging. This task is extremely time-consuming due to high image complexity and volume. However, it is important to facilitate the early detection of breast cancer for medical intervention. Deep learning (DL) has become popular in medical imaging solutions and has demonstrated various levels of performance in diagnosing cancerous images. Nonetheless, achieving high precision while minimizing overfitting remains a significant challenge for classification solutions. The handling of imbalanced data and incorrect labeling is a further concern. Additional methods, such as pre-processing, ensemble, and normalization techniques, have been established to enhance image characteristics. These methods could influence classification solutions and be used to overcome overfitting and data balancing issues. Hence, developing a more sophisticated DL variant could improve classification accuracy while reducing overfitting. Technological advancements in DL have fueled automated breast cancer diagnosis growth in recent years. This paper reviewed studies on the capability of DL to classify histopathological breast cancer images, as the objective of this study was to systematically review and analyze current research on the classification of histopathological images. Additionally, literature from the Scopus and Web of Science (WOS) indexes was reviewed. This study assessed recent approaches for histopathological breast cancer image classification in DL applications for papers published up until November 2022. The findings of this study suggest that DL methods, especially convolution neural networks and their hybrids, are the most cutting-edge approaches currently in use. To find a new technique, it is necessary first to survey the landscape of existing DL approaches and their hybrid methods to conduct comparisons and case studies.

Filters

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links