Displaying publications 1161 - 1180 of 1808 in total

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  1. Occurrence, distribution, and ecological risk of bisphenol analogues in marine ecosystem of urbanized coast and estuary
    Zainuddin AH, Roslan MQJ, Razak MR, Yusoff FM, Haron DEM, Aris AZ
    Mar Pollut Bull, 2023 Jul;192:115019.
    PMID: 37201347 DOI: 10.1016/j.marpolbul.2023.115019
    Bisphenol analogues are prevalent globally because of rampant usage and imprecise processing techniques, prompting alerts about environmental and health hazards. The method employed in this study by solid phase extraction (SPE) and liquid chromatography-tandem quadrupole mass spectrometer (LC-MS/MS) for both quantification and qualitative analysis of the bisphenol compounds in the surface water samples. The coastal and estuarine surface water of Port Dickson and Lukut ranges from 1.32 ng/L to 1890.51 ng/L of bisphenol analogues. BPF mean concentration at 1143.88 ng/L is the highest, followed by BPA and BPS at 59.01 ng/L and 10.96 ng/L, respectively. Based on RQm for bisphenol analogues, the highest for BPF at 2.49 (RQ > 1, high risk), followed by BPS at 0.12 (0.1 
  2. Discovery of a novel dual functional phenylpyrazole-styryl hybrid that induces apoptotic and autophagic cell death in bladder cancer cells
    Leong SW, Wang J, Okuda KS, Su Q, Zhang Y, Abas F, et al.
    Eur J Med Chem, 2023 Apr 20;254:115335.
    PMID: 37098306 DOI: 10.1016/j.ejmech.2023.115335
    Unpleasant side effects and resistance development remained the Achilles heel of chemotherapy. Since low tumor-selectivity and monotonous effect of chemotherapy are closely related to such bottleneck, targeting tumor-selective multi-functional anticancer agents may be an ideal strategy in the search of new safer drugs. Herein, we report the discovery of compound 21, a nitro-substituted 1,5-diphenyl-3-styryl-1H-pyrazole that possesses dual functional characteristics. The 2D- and 3D-culture-based studies revealed that 21 not only could induce ROS-independent apoptotic and EGFR/AKT/mTOR-mediated autophagic cell deaths in EJ28 cells simultaneously but also has the ability in inducing cell death at both proliferating and quiescent zones of EJ28 spheroids. The molecular modelling analysis showed that 21 possesses EGFR targeting capability as it forms stable interactions in the EGFR active site. Together with its good safety profile in the zebrafish-based model, the present study showed that 21 is promising and may lead to the discovery of tumor-selective multi-functional anti-cancer agents.
  3. Fulltext Hydrogen gas sensing performance of a carbon-doped boron nitride nanoribbon at elevated temperatures
    Taib AK, Johari Z, Abd Rahman SF, Mohd Yusoff MF, Hamzah A
    PLoS One, 2023;18(3):e0282370.
    PMID: 36897883 DOI: 10.1371/journal.pone.0282370
    In this study, computational simulations were used to investigate the performance of a carbon-doped boron nitride nanoribbon (BC2NNR) for hydrogen (H2) gas sensing at elevated temperatures. The adsorption energy and charge transfer were calculated when H2 was simultaneously attached to carbon, boron, and both boron and nitrogen atoms. The sensing ability was further analyzed considering the variations in current-voltage (I-V) characteristics. The simulation results indicated that the energy bandgap of H2 on carbon, boron, and both boron and nitrogen exhibited a marginal effect during temperature variations. However, significant differences were observed in terms of adsorption energy at a temperature of 500 K, wherein the adsorption energy was increased by 99.62% of that observed at 298 K. Additionally, the evaluation of charge transfer indicated that the strongest binding site was achieved at high adsorption energies with high charge transfers. Analysis of the I-V characteristics verified that the currents were considerably affected, particularly when a certain concentration of H2 molecules was added at the highest sensitivity of 15.02% with a bias voltage of 3 V. The sensitivity at 298 K was lower than those observed at 500 and 1000 K. The study findings can form the basis for further experimental investigations on BC2NNR as a hydrogen sensor.
  4. Fulltext Curcumin improves the efficacy of cisplatin by targeting cancer stem-like cells through p21 and cyclin D1-mediated tumour cell inhibition in non-small cell lung cancer cell lines
    Baharuddin P, Satar N, Fakiruddin KS, Zakaria N, Lim MN, Yusoff NM, et al.
    Oncol Rep, 2016 Jan;35(1):13-25.
    PMID: 26531053 DOI: 10.3892/or.2015.4371
    Natural compounds such as curcumin have the ability to enhance the therapeutic effectiveness of common chemotherapy agents through cancer stem-like cell (CSC) sensitisation. In the present study, we showed that curcumin enhanced the sensitivity of the double-positive (CD166+/EpCAM+) CSC subpopulation in non-small cell lung cancer (NSCLC) cell lines (A549 and H2170) to cisplatin-induced apoptosis and inhibition of metastasis. Our results revealed that initial exposure of NSCLC cell lines to curcumin (10-40 µM) markedly reduced the percentage of viability to an average of ~51 and ~54% compared to treatment with low dose cisplatin (3 µM) with only 94 and 86% in both the A549 and H2170 cells. Moreover, sensitisation of NSCLC cell lines to curcumin through combined treatment enhanced the single effect induced by low dose cisplatin on the apoptosis of the double-positive CSC subpopulation by 18 and 20% in the A549 and H2170 cells, respectively. Furthermore, we found that curcumin enhanced the inhibitory effects of cisplatin on the highly migratory CD166+/EpCAM+ subpopulation, marked by a reduction in cell migration to 9 and 21% in the A549 and H2170 cells, respectively, indicating that curcumin may increase the sensitivity of CSCs to cisplatin-induced migratory inhibition. We also observed that the mRNA expression of cyclin D1 was downregulated, while a substantial increased in p21 expression was noted, followed by Apaf1 and caspase-9 activation in the double-positive (CD166+/EpCAM+) CSC subpopulation of A549 cells, suggested that the combined treatments induced cell cycle arrest, therefore triggering CSC growth inhibition via the intrinsic apoptotic pathway. In conclusion, we provided novel evidence of the previously unknown therapeutic effects of curcumin, either alone or in combination with cisplatin on the inhibition of the CD166+/EpCAM+ subpopulation of NSCLC cell lines. This finding demonstrated the potential therapeutic approach of using curcumin that may enhance the effects of cisplatin by targeting the CSC subpopulation in NSCLC.
  5. Evaluation of the biomechanics of Aramany class I obturators of different designs using numerical and experimental methods. Part I: Retention and associated stress
    Mousa MA, Husein A, El-Anwar MI, Yusoff N, Abdullah JY
    J Prosthet Dent, 2024 Jul 23.
    PMID: 39048390 DOI: 10.1016/j.prosdent.2024.07.011
    STATEMENT OF PROBLEM: Studies on the biomechanics of obturators in the currently used designs of Aramany class I defect are lacking. Also, modifications of the designs presently used in unilateral palatal defects are needed to produce a prosthesis with more retention and less stress on the abutments.

    PURPOSE: The purpose of part I of this study was to differentiate among Aramany class I obturators of 4 designs regarding retention and associated stress using numerical and experimental methods.

    MATERIAL AND METHODS: Four finite element models and 36 different base obturators were fabricated and divided into 9 acrylic resin bases retained with Adams clasps and 9 linear, 9 tripodal, and 9 fully tripodal design obturators from casts obtained from a scanned skull. After modification, the prostheses were fabricated on the casts obtained from a 3-dimensionally printed cast. The retention was evaluated, and the data were collected and analyzed using a statistical software program (α=.05). The displacement and associated stress in the assorted casts were compared by using 5-N displacing force at 3 points using finite element analysis. The quantitative assessment was made by measuring the displacement and von Mises stress distribution on the prostheses and their supporting structures. The qualitative analysis was done by using a visual color mapping to depict stress location and intensity.

    RESULTS: No significant differences were found between fully tripodal (4.478 ±2.303 MPa) and tripodal obturators (4.478 ±2.286 MPa; P=.153), although fully tripodal showed more resistance to anterior displacement (4.522 ±0.979 and 3.553 ±1.58 MPa for fully tripodal and tripodal designs, respectively; P=.007), and tripodal obturators produced more resistance to middle displacement (5.441 ±1.778 and 2.784 ±0.432 MPa for tripodal and fully tripodal design respectively; P=.001). The fully tripodal obturator showed more retention (3.736 ±1.182 MPa) than the linear one (2.493 ±1.052 MPa; P=.001). The maxillary central incisor was the most stressed abutment, followed by the lateral incisor, while the second molar was the least.

    CONCLUSIONS: Regarding retention, the fully tripodal obturator produces retention comparable with the tripodal and significantly more than the linear. Acrylic resin prostheses retained with Adams clasps may be similar to metal-based prostheses regarding retention and stress distribution on the supporting structures.

  6. Eco-friendly encapsulation: Investigating plant-based protein-alginate shells for efficient delivery and digestion of hemp seed oil encapsulated via supercritical CO2 dispersion
    Gholivand S, Tan TB, Yusoff MM, Qoms MS, Wang Y, Liu Y, et al.
    Food Chem, 2025 Jan 15;463(Pt 4):141515.
    PMID: 39395350 DOI: 10.1016/j.foodchem.2024.141515
    In this study, supercritical carbon dioxide solution-enhanced dispersion (SEDS) was used to encapsulate hemp seed oil (HSO) within matrices of hemp seed protein isolate (HPI), pea protein (PPI) and soy protein (SPI) (0.5 % w/v) in complex with alginate (AL) (0.01 % w/v). The effects of different pH levels (3-9), NaCl concentrations (0-200 mmol/L) and simulated gastrointestinal conditions on HSO release and digestion patterns were analyzed. The findings revealed that SPI/AL microcapsules effectively maintained structural integrity and controlled oil release across diverse pH levels and salt concentrations. During gastrointestinal phases, minimal oil release was observed during oral digestion (<25 % for all samples), while significant (P 
  7. Fulltext A Comparison of the National Cervical Cancer Policies in Six Developing Countries with the World Health Organization Recommendations: A Narrative Review
    Amir SM, Idris IB, Said ZM, Yusoff HM, Manaf MRA
    Iran J Public Health, 2023 Jun;52(6):1108-1120.
    PMID: 37484154 DOI: 10.18502/ijph.v52i6.12952
    BACKGROUND: This study reviewed cervical cancer policies implemented in developing countries that adhered to WHO standards. Despite long being known as a preventable disease, cervical cancer is still one of the leading causes of mortality among women. Nine out of ten cervical cancer deaths occurred in less-developed countries, suggesting that the preventive and control approaches in these countries might differ from those in developed nations.

    METHODS: Cervical cancer policies in six developing countries from each WHO region were selected while related data from the Cervical Cancer Country Profiles 2021 by WHO were retrieved for comparison.

    RESULTS: The cervical cancer policies that were included in this review were from Malaysia, Thailand, Iran, Kenya, Argentina, and Ukraine. According to the latest guidelines on the management of cervical cancers, WHO recommendations have been elaborated on primary, secondary, and tertiary prevention. A comparison of policies among these selected countries showed variation in each level of prevention. The cancer burden in each country was also found to determine the progression of cervical cancer prevention and policy controls in these countries.

    CONCLUSION: This review emphasizes the dissimilarities of cervical cancer policies in six developing countries compared to recommendations by the WHO. Identifying these discrepancies could help policymakers in developing nations to recognize the pressing issues surrounding cervical cancer prevention and establish more effective prevention and control approaches.

  8. Valorization of seafood waste: A review of life cycle assessment studies in biorefinery applications
    Yusoff MA, Mohammdi P, Ahmad F, Sanusi NA, Hosseinzadeh-Bandbafha H, Vatanparast H, et al.
    Sci Total Environ, 2024 Aug 26.
    PMID: 39197788 DOI: 10.1016/j.scitotenv.2024.175810
    The escalating challenges posed by seafood waste generated by the fishing and aquaculture industries underscore the urgent need for innovative solutions that promote both environmental conservation and economic viability within the seafood sector. Seafood waste biorefinery emerges as a promising solution, offering the potential to transform waste materials into valuable products. However, it is essential to recognize that seafood waste biorefinery operations also entail environmental impacts that warrant careful consideration. Environmental assessment tools like Life Cycle Assessment (LCA) provide a valuable framework for assessing these impacts comprehensively. This review critically examines LCA studies in seafood waste biorefinery, focusing on key concepts, emerging technologies, and potential product avenues. Despite the growing body of research in this area, direct comparisons between published studies prove challenging due to discrepancies in feedstocks, processing techniques, value-added products, and LCA methodologies. Nevertheless, the findings consistently demonstrate significant reductions in environmental impacts achieved through seafood waste biorefinery processes. The selection of technologies significantly influences both product quality and sustainability measures. High energy consumption, including diesel fuel consumption in fishing vessels and electricity consumption in processing steps, should be carefully considered and reduced to mitigate associated environmental impacts. In conclusion, while seafood waste biorefinery processes hold significant promise for providing environmental and economic benefits, substantial challenges remain. This review provides invaluable insights for researchers, policymakers, and stakeholders, emphasizing the importance of continuous interdisciplinary collaboration and methodological standardization to advance sustainable waste management practices in the seafood industry.
  9. Fulltext Physical Activity in Malaysia: Are We Doing Enough? Findings from the REDISCOVER Study
    Nik-Nasir NM, Md-Yasin M, Ariffin F, Mat-Nasir N, Miskan M, Abu-Bakar N, et al.
    Int J Environ Res Public Health, 2022 Dec 15;19(24).
    PMID: 36554769 DOI: 10.3390/ijerph192416888
    Physical activity (PA) in the form of structured or unstructured exercise is beneficial for health. This paper aims to study PA levels across four domains according to the International Physical Activity Questionnaire (IPAQ) and its associated factors. A total of 7479 Malaysian adult participants between 18 to 90 years old from the REDISCOVER study who completed the IPAQ were analyzed. PA was calculated as MET-min per week and were categorized according to insufficiently active, sufficiently active and very active. Multinomial regression was used to determine the association between sociodemographic, clinical factors and the level of PA. The mean age of the participants was 51.68 (±9.5 SD). The total reported physical activity in median (IQR) was 1584.0 (0-5637.3) MET-min per week. The highest total for PA was in the domestic domain which is 490 (0-2400) MET-min per week. Factors associated with sufficiently active or very active PA include Malay ethnicity, no formal education, elementary occupation, current smokers and high HDL. Whereas low income, male and normal BMI are less likely to participate in sufficiently active or very active PA. Intervention to encourage higher PA levels in all domains is important to achieve recommended PA targets.
  10. Abundance of microplastics and its ecological risk assessment in coral reef regions of Peninsular Malaysia
    Lim KP, Sun C, Yusoff S, Ding J, Loh KH, Li J, et al.
    Mar Pollut Bull, 2024 Dec;209(Pt A):117112.
    PMID: 39406069 DOI: 10.1016/j.marpolbul.2024.117112
    Microplastic contamination is an emerging concern in marine ecosystems, with limited knowledge on its impact on coral reefs, particularly in Malaysia. Surface waters were collected from several coral reef regions in Peninsular Malaysia by towing a plankton net behind the boat. Microplastics were detected at all sites, with a mean abundance of 0.344 ± 0.457 MP/m3. Perhentian Islands (0.683 ± 0.647 MP/m3) had significantly higher microplastic levels than Tioman Island (0.108 ± 0.063 MP/m3), likely due to oceanographic differences. Over half of the microplastics (55.7 %) were small microplastics (<1 mm), with the 0.05-0.5 mm size class being most abundant (29.2 %). Fragments and fibres dominated, and black, blue, and green were the prevalent colours. Polyethylene (PE), rayon (RY), chlorinated polyethylene (CPE), and polypropylene (PP) were the most common polymers. This study reveals the abundance and characteristics of microplastics, provides important data for further research on microplastics in coral reef ecosystem.
  11. Fulltext Prediction equations for lung function in healthy, life time never-smoking Malaysian population
    Gnanou J, Caszo B, Mohamad WH, Nawawi H, Yusoff K, Ismail T
    Southeast Asian J Trop Med Public Health, 2011 Jul;42(4):965-76.
    PMID: 22299480
    Several equations have been used to predict lung function standard results for different populations. It is important lung function evaluations use appropriate standards for the study population. The objective of this study was to develop a prediction equation for lung function test results for the Malaysian population. Spirometry was performed among 5,708 subjects and 1,483 healthy, lifetime never smoked subjects (386 males and 1,097 females). Prediction equations were derived for both men and women for FVC and FEV1 results. The equations were validated on new subjects (n = 532, 222 males and 310 females) who met the same inclusion and exclusion criteria as the main cohort. There was a positive correlation between the measured values and the values derived from the new prediction equations (0.62 for FEV1 and between 0.66 and 0.67 for FVC; both p < 0.05) for both men and women with a smaller bias and limit of agreement compared to the published reference equations of ECCS, Knudson, Crapo and NHANES III. The reference equations derived from local spirometry data were more appropriate than generally used equations based on data from previous studies in different population.
  12. Polypeptides associated with in vitro cyst formation of Blastocystis hominis
    Init I, Mak JW, Top S, Zulhainan Z, Prummongkol S, Nissapatorn V, et al.
    Southeast Asian J Trop Med Public Health, 2003 Dec;34(4):727-32.
    PMID: 15115079
    The objective of this study was to characterize the polypeptides associated with cysts of Blastocystis hominis. This form is believed to be infective and plays a role in parasite resistance to anti-B. hominis drugs currently used for treatment of Blastocystis associated diarrhea. Cysts were induced through in vitro culture of the parasite in complete medium supplemented with bacterial extract with trypticase, metronidazole or doxycycline. SDS-PAGE analysis showed almost similar polypeptide patterns of parasite extracts obtained from in vitro cultured parasites before and after exposure with the three supplements. Polypeptide bands at 76, 58.5, 48, 45, 40, 38, 32, 25 and 22 kDa were constantly seen in all antigenic preparations and no specific cyst-associated polypeptide was present. However, on immunoblot analysis, 3 out of 16 blastocystosis human sera identified a cyst-associated polypeptide at 60 kDa in all parasite extracts prepared from cultures with the three supplements. In addition, there were associated morphological changes detected in these parasites stained with acridine orange and observed under fluorescence microscopy. Metronidazole induced cyst forms (reddish cells) as early as 12 hours post-exposure; more cyst production (with stronger immunoblot bands) occurred after 24 hours exposure. However, cysts rupture with release and destruction of B. hominis daughters cells occurred after 48 hours exposure. Doxycycline induced less cyst-like forms at 24 hours (weaker 60 kDa band) and less destruction of the cysts (60 kDa band still present at 72 hours post exposure). Bacterial extract and trypticase also induced cysts at 12 hours with increasing numbers up to 72 hours exposure (corresponding increase in intensity of 60 kDa band from samples harvested at 12 to 72 hours post exposure) without any sign of deleterious effect on the parasite.
  13. Fulltext G6PD Viangchan and G6PD Mediterranean are the main variants in G6PD deficiency in the Malay population of Malaysia
    Mohd Yusoff N, Shirakawa T, Nishiyama K, Choo KE, Isa MN, Matsuo M
    Southeast Asian J Trop Med Public Health, 2003;34 Suppl 3:135-7.
    PMID: 15906717
    Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked red blood cell enzymopathy common in malaria endemic areas. Individuals affected by this disease show a wide variety of clinical signs of acute hemolytic anemia. Mutations of the G6PD gene in the Malay population with G6PD deficiency in Kelantan, a state in North East Malaysia were studied. Ninety-three individuals with G6PD deficiency were subjected to mutation analysis of the G6PD gene using polymerase chain reaction based techniques of multiplex PCR. Of the ninety-three DNA samples studied, molecular defects were identified in 80 cases (86%). Variants were heterogeneous - 28.7% were found to have a G to A nucleotide change at nucleotide 871 of the G6PD gene (G871A), corresponding to G6PD Viangchan. The other major mutations were G6PD Mediterranean, G6PD Vanua Lava, G6PD Coimbra, G6PD Kaiping, G6PD Orissa, G6PD Mahidol, G6PD Canton and G6PD Chatham. These results showed that there are heterogeneous mutations of the G6PD gene associated with G6PD deficiency and that G6PD Viangchan and G6PD Mediterranean account for the main variants in G6PD deficiency among the Malay population in Malaysia.
  14. Fulltext The pattern of reporting and presenting validity evidence of extended matching questions (EMQs) in health professions education: a systematic review
    Taha MH, Mohammed HEEG, Abdalla ME, Yusoff MSB, Mohd Napiah MK, Wadi MM
    Med Educ Online, 2024 Dec 31;29(1):2412392.
    PMID: 39445670 DOI: 10.1080/10872981.2024.2412392
    The Extended matching Questions (EMQs), or R-type questions, are format of selected-response. The validity evidence for this format is crucial, but there have been reports of misunderstandings about validity. It is unclear what kinds of evidence should be presented and how to present them to support their educational impact. This review explores the pattern and quality of reporting the sources of validity evidence of EMQs in health professions education, encompassing content, response process, internal structure, relationship to other variables, and consequences. A systematic search in the electronic databases including MEDLINE via PubMed, Scopus, Web of Science, CINAHL, and ERIC was conducted to extract studies that utilize EMQs. The framework for a unitary concept of validity was applied to extract data. A total of 218 titles were initially selected, the final number of titles was 19. The most reported pieces of evidence were the reliability coefficient, followed by the relationship to another variable. Additionally, the adopted definition of validity is mostly the old tripartite concept. This study found that reporting and presenting validity evidence appeared to be deficient. The available evidence can hardly provide a strong validity argument that supports the educational impact of EMQs. This review calls for more work on developing a tool to measure the reporting and presenting validity evidence.
  15. Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR Green I real-time PCR with nucleocapsid gene-specific primers
    Tan SW, Ideris A, Omar AR, Yusoff K, Hair-Bejo M
    J Virol Methods, 2009 Sep;160(1-2):149-56.
    PMID: 19447142 DOI: 10.1016/j.jviromet.2009.05.006
    SYBR Green I real-time PCR was developed for detection and differentiation of Newcastle disease virus (NDV). Primers based on the nucleocapsid (NP) gene were designed to detect specific sequence of velogenic strains and lentogenic/vaccine strains, respectively. The assay was developed and tested with NDV strains which were characterized previously. The velogenic strains were detected only by using velogenic-specific primers with a threshold cycle (C(t)) 18.19+/-3.63 and a melting temperature (T(m)) 86.0+/-0.28 degrees C. All the lentogenic/vaccine strains, in contrast, were detected only when lentogenic-specific primers were used, with the C(t) value 14.70+/-2.32 and T(m) 87.4+/-0.21 degrees C. The assay had a dynamic detection range which spans over a 5log(10) concentration range, 10(9)-10(5) copies of DNA plasmid/reaction. The velogenic and lentogenic amplifications showed high PCR efficiency of 100% and 104%, respectively. The velogenic and lentogenic amplifications were highly reproducible with assay variability 0.45+/-0.31% and 1.30+/-0.65%, respectively. The SYBR Green I real-time PCR assay detected successfully the virus from tissue samples and oral swabs collected from the velogenic and lentogenic NDV experimental infection, respectively. In addition, the assay detected and differentiated accurately NDV pathotypes from suspected field samples where the results were in good agreement with both virus isolation and analysis of the fusion (F) cleavage site sequence. The assay offers an attractive alternative method for the diagnosis of NDV.
  16. Isolation and molecular identification of Nipah virus from pigs
    AbuBakar S, Chang LY, Ali AR, Sharifah SH, Yusoff K, Zamrod Z
    Emerg Infect Dis, 2004 Dec;10(12):2228-30.
    PMID: 15663869
    Nipah viruses from pigs from a Malaysian 1998 outbreak were isolated and sequenced. At least two different Nipah virus strains, including a previously unreported strain, were identified. The findings highlight the possibility that the Malaysia outbreaks had two origins of Nipah virus infections.
  17. Synthesis, anti-amoebic activity and molecular docking simulation of eugenol derivatives against Acanthamoeba sp
    Zamli KM, Hashim F, Razali SA, Yusoff HM, Mohamad H, Abdullah F, et al.
    Saudi Pharm J, 2023 Sep;31(9):101703.
    PMID: 37546528 DOI: 10.1016/j.jsps.2023.101703
    Amoebae of the genus Acanthamoeba can cause diseases such as amoebic keratitis and granulomatous amoebic encephalitis. Until now, treatment options for these diseases have not been fully effective and have several drawbacks. Therefore, research into new drugs is needed for more effective treatment of Acanthamoeba infections. Eugenol, a phenolic aromatic compound mainly derived from cloves, has a variety of pharmaceutical properties. In this study, nine eugenol derivatives (K1-K9), consisting of five new and four known compounds, were synthesized and screened for their antiamoebic properties against Acanthamoeba sp. The structure of these compounds was characterized spectroscopically by Fourier transform infrared (FTIR), Ultraviolet-Visible (UV-Vis), 1H and 13C Nuclear Magnetic Resonance (NMR) and mass spectrometer (MS). The derived molecules were screened for antiamoebic activity by determining IC50 values based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and observation of amoeba morphological changes by light and fluorescence microscopy. Most of the tested compounds possessed strong to moderate cytotoxic effects against trophozoite cells with IC50 values ranging from 0.61 to 24.83 μg/mL. Observation of amoebae morphology by light microscopy showed that the compounds caused the transformed cells to be roundish and reduced in size. Furthermore, fluorescence microscopy observation using acridine orange (AO) and propidium iodide (PI) (AO/PI) staining showed that the cells have damaged membranes by displaying a green cytoplasm with orange-stained lysosomes. Acidification of the lysosomal structure indicated disruption of the internal structure of Acanthamoeba cells when treated with eugenol derivatives. The observed biological results were also confirmed by interaction simulations based on molecular docking between eugenol derivatives and Acanthamoeba profilin. These interactions could affect the actin-binding ability of the protein, disrupting the shape and mobility of Acanthamoeba. The overall results of this study demonstrate that eugenol derivatives can be considered as potential drugs against infections caused by Acanthamoeba.
  18. Fulltext Production and differential activity of recombinant human wild-type G6PD and G6PDViangchan
    Vengidasan L, Yunus MA, Yusoff NM, Yahaya BH, Ismail IS
    Asian Biomed (Res Rev News), 2020 Aug;14(4):159-167.
    PMID: 37551388 DOI: 10.1515/abm-2020-0023
    BACKGROUND: Glucose-6-phosphate dehydrogenase (G6PD) is essential to produce reduced nicotinamide adenine dinucleotide phosphate, which is required to protect cells against oxidative stress. G6PD deficiency is a genetic variation that may lead to hemolysis with potential consequences, such as kidney failure, and patients often experience low quality of life.

    OBJECTIVES: To establish a simple, efficient, and optimized method to produce a G6PDViangchan variant and characterize the phenotypes of recombinant human wild-type G6PD and G6PDViangchan.

    METHODS: G6PD was amplified by polymerase chain reaction (PCR) from a human cDNA plasmid, and the gene for G6PDViangchan was amplified by initiating a mutation at location 871 (G>A) through site-directed mutagenesis. Protein expression and western blotting were conducted after successful cloning. The enzymatic activity of both proteins was assessed spectrophotometrically after purification.

    RESULTS: Both amplicons were successfully cloned into a pET26b(+) expression vector and transformed into Escherichia coli BL21 (DE3) cells for overexpression as C-terminally histidine-tagged recombinant proteins. Western blotting confirmed that both proteins were successfully produced at similar levels. The enzymes were purified by immobilized metal (Co) affinity chromatography. Postpurification assay of enzyme activity revealed about 2-fold differences in the levels of specific activity between the wild-type G6PD (155.88 U/mg) and G6PDViangchan (81.85 U/mg), which is consistent with earlier reports. Analysis in silico showed that the coding change in G6PDViangchan has a substantial effect on protein folding structure.

    CONCLUSIONS: We successfully cloned, expressed, and purified both wild-type G6PD and G6PDViangchan proteins. Such a protocol may be useful for creating a model system to study G6PD deficiency disease.

  19. Fulltext Experimental Study on Cementless PET Mortar with Marble Powder and Iron Slag as an Aggregate
    Khan SU, Rahim A, Md Yusoff NI, Khan AH, Tabassum S
    Materials (Basel), 2023 Jul 27;16(15).
    PMID: 37569974 DOI: 10.3390/ma16155267
    There has been an increase in plastic production during the past decades, yet the recycling of plastic remains relatively low. Incorporating plastic in concrete can mitigate environmental pollution. The use of waste polyethylene terephthalate (PET) bottles as an aggregate weakens properties of concrete. An alternative is to use PET bottles as a binder in the mortar. The PET binder mixed with sand results in weak mortar. Marble and iron slag can enhance PET mortar properties by preventing alkali reactions. This study examines the mechanical and durability properties of PET mortar with different mixes. The mixes were prepared as plastic and marble (PM); plastic and iron slag (PI); plastic, sand, and marble (PSM); plastic, iron slag, and marble (PIM); and plastic, sand, and iron slag (PSI). PM with 30-45% plastic content had increased compressive and flexural strength up to 35.73% and 20.21%, respectively. PI with 30-35% plastic content showed strength improvements up to 29.19% and 5.02%, respectively. However, at 45% plastic content, strength decreased by 8.8% and 27.90%. PSM, PIM, and PSI specimens had nearly double the strength of ordinary Portland cement (OPC) mortar. The durability of PET mortar in chemical solutions, mainly 5% HCl and 20% NaOH, indicate that mass decreased after 3, 7, and 28 days. All specimens showed good resistance to HCl and NaCl solutions compared to OPC mortar. However, its resistance to NaOH is low compared to OPC mortar. PET mortar without cement showed higher strength and durability than cement mortar, making it suitable for paver tiles, drainage systems, and roads.
  20. Interaction of low-level dietary supplementation of Chlorella vulgaris Beijerinck, 1890, and feeding duration on growth hormone, growth performance and serum biochemistry of red hybrid tilapia (Oreochromis mossambicus × Oreochromis niloticus)
    Ahmad MT, Shariff M, Goh YM, Banerjee S, Yusoff FM
    J Fish Biol, 2023 Sep;103(3):715-726.
    PMID: 37249562 DOI: 10.1111/jfb.15469
    Chlorella is one of the most widely accepted Chlorophyta used by many as livestock and aquaculture feed. Nonetheless, different studies on the overall performances of fish reported the unfavourable effect of high-level supplementations of Chlorella vulgaris. The current study determined the impact of low-level dietary supplementation of C. vulgaris alongside the different feeding durations and their interactions on the growth hormone (GH), growth performances, serum-biochemical indices, hepatic function and some immunological parameters of red hybrid tilapia. The fingerlings (mean weight: 14.25 ± 0.01 g, length: 13.5 ± 0.49 cm) were fed diets containing 0, 0.99%, 2.91% and 4.76% of C. vulgaris powder per kilogram dry diet for 90 days. GH, growth performance, serum-biochemical indices (total serum protein, albumin, globulin, glucose, aspartate aminotransferase and alanine aminotransferase) and some immunological (respiratory burst and lysozyme activities) parameters of the fish were examined after 30, 60 and 90 days of feeding. The results demonstrated that tilapia fed C. vulgaris-supplemented diets showed increased levels of respiratory burst, lysozyme, albumin and total protein, GH and growth performances (P 
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