METHOD: This study collected SCL-90 Scale test results from 5262 students (4012 boys and 1250 girls) through a questionnaire survey and conducted a Sport Quality Test on these students. Statistical software SPSS was applied for differential analysis and logistic regression analysis. Specifically, the differences in sport quality indicators between normal and abnormal mean total scores of psychological tests were analyzed first. Then, the binary logistic regression model was used to explore the impacts of sports quality indicator scores on students' psychological fitness.
RESULTS: There are differences in the results of physical fitness tests between students with abnormal psychology and students with normal psychology. The four indexes of students' vital capacity, speed, explosive power of lower limbs, and endurance running are effective in improving students' psychologically abnormal state, and endurance running and improving vital capacity are the most effective methods to improve students' psychologically abnormal state. In the physical tests of Chinese college students, the risk of psychological abnormalities was reduced by 9% for every one-point increase in lung capacity and 10.4% for every one-point increase in endurance running performance.
CONCLUSIONS: Chinese college students' physical fitness and mental health are related. The best methods for treating psychological disorders are lung capacity improvement and endurance running. According to the physical test results of Chinese college students, for every 1-point increase in lung capacity and endurance running, the risk of psychological abnormalities decreased by 9% and 10.4%, respectively.
METHODS: In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology. The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC). At the same time, the antioxidant activity of ALPs was investigated by in vitro antioxidant experiment.
RESULTS: The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%. FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface. The ALPs were chiefly composed of glucose, rhamnose, arabinose, and galactose with a molar ratio of 70.19:10.95:11.16:6.90. In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
DISCUSSION: The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides. These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
METHODS: This paper first discusses the correlation between the characteristics of visual-spatial impairments and environmental factors and then investigates the color preferences of such patients based on the CIE 1976 color system and the Analytic Hierarchy Process (AHP). Subsequently, the paper explores spatial design strategies conducive to spatial orientation from the perspective of adaptability to pathological characteristics, utilizing case study analysis.
RESULTS: (1) Pathological characteristics of visual-spatial impairments (such as difficulties in spatial orientation and spatial neglect) are related to environmental factors; (2) Emotional attachment factors play a key role in patients' perception of satisfaction with environmental colors; (3) Color associations have the potential to strengthen spatial memory. Additionally, interface designs with high luminance, low saturation, and clear color differentiation facilitate patients' recognition of space.
DISCUSSION: This paper posits that spatial interface design is a feasible approach to assist with spatial orientation, and it achieves this through a mediating process that progresses from influencing visual stimuli to cognitive memory and then to behavioral orientation. The article provides insights into the operational feasibility of this method.
METHODS: We conducted a comprehensive search on March 15, 2024, using Web of Science, PubMed, Scopus, and EBSCOhost. Eligibility criteria for selecting studies were established based on the PICOS framework: (i) Population-healthy soccer players; (ii) Intervention-SAQ training; (iii) Comparison condition (conventional training or traditional training); (iv) Outcome-physical performance (speed, agility, strength, etc.); (v) Study design-randomized controlled trials. The PEDro scale was employed to evaluate the methodological quality of each study, and a random-effects model was used for the meta-analysis.
RESULTS: A total of 11 studies met the inclusion criteria for the systematic literature review. One study with low PEDro score was excluded, and one was excluded based on Cochrane bias risk assessment. Finally, 9 studies were included in the meta-analysis, comprising 498 soccer players. Overall, the results indicated a significant impact of SAQ training on physical qualities and dribbling speed among soccer players. Specifically, there was a moderate effect size for sprint performance (5m, 10m, 20m) (ES = 0.75; p < 0.01), change of direction ability (COD) (ES = 0.35; p < 0.001), power (vertical and horizontal jumps) (ES = 0.67; p < 0.01), while flexibility showed no significant impact (ES = 0.11; p > 0.05). Moreover, change-of-direction dribbling demonstrated a significant effect (ES = 0.58; p < 0.01).
CONCLUSION: Overall, SAQ training effectively enhances speed, COD, explosiveness, and change-of-direction dribbling specific performance in adolescent soccer players, particularly in sprinting. However, it does not have an advantage in improving flexibility. Further high-quality studies encompassing a broader range of exercises are needed to fully determine the effectiveness of SAQ training in improving other physical qualities and technical skills of soccer players, as well as ultimately enhancing match performance.
METHODS: A thorough search of databases, including Web of Science Core, SPORTDiscus, PubMed, and SCOPUS, was conducted, with data up to July 2024. The PEDro scale assessed study quality and RevMan 5.3 evaluated bias risk. Effect sizes (ES) were calculated from means and standard deviations, with heterogeneity assessed using the I² statistic. Publication bias was evaluated using an extended Egger test.
RESULTS: Eleven RCTs involving 499 healthy athletes from sports such as soccer, basketball, tennis, and handball met the inclusion criteria. The SAQ interventions lasted between 4 and 12 weeks, with a frequency of two to three sessions per week. The analysis revealed significant improvements in 5-meter sprint (ES = 0.63, p 60 min: ES = 0.24; p = 0.059).
CONCLUSIONS: SAQ training effectively enhances sprint performance, COD ability, reaction time, lower limb strength, and flexibility, with effect sizes ranging from small to moderate. The findings suggest that shorter training durations (≤ 60 min) may be more beneficial for improving COD performance, although this effect did not reach statistical significance. Further trials are recommended to determine the optimal training dosage, along with high-quality studies covering a broader range of sports, particularly in athletes aged 14 to 18.
METHODS: This study innovatively explores the potential of H. illucens larvae (HIL) protein as a peptone substitute for microbial culture media. Four commercial proteases (alkaline protease, trypsin, trypsase, and papain) were explored to hydrolyze the defatted HIL, and the experimental conditions were optimized via response surface methodology experimental design. The hydrolysate of the defatted HIL was subsequently vacuum freeze-dried and deployed as a growth medium for three bacterial strains (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) to determine the growth kinetics between the HIL peptone and commercial peptone.
RESULTS: The optimal conditions were 1.70% w/w complex enzyme (alkaline protease: trypsin at 1:1 ratio) at pH 7.0 and 54 °C for a duration of 4 h. Under these conditions, the hydrolysis of defatted HIL yielded 19.25% ±0.49%. A growth kinetic analysis showed no significant difference in growth parameters (μmax, Xmax, and λ) between the HIL peptone and commercial peptone, demonstrating that the HIL hydrolysate could serve as an effective, low-cost alternative to commercial peptone. This study introduces an innovative approach to HIL protein resource utilization, broadening its application beyond its current use in animal feed.
METHODS: Through the Asia-Pacific Hepatocellular Carcinoma trials group (NCT03267641), we recruited one of the largest prospective cohorts of patients with HCC, with over 600 whole genome and transcriptome samples from 123 treatment-naïve patients.
RESULTS: Using a multi-region sampling approach, we revealed seven convergent genetic evolutionary paths governed by the early driver mutations, late copy number variations and viral integrations, which stratify patient clinical trajectories after surgical resection. Furthermore, such evolutionary paths shaped the molecular profiles, leading to distinct transcriptomic subtypes. Most significantly, although we found the coexistence of multiple transcriptomic subtypes within certain tumors, patient prognosis was best predicted by the most aggressive cell fraction of the tumor, rather than by overall degree of transcriptomic ITH level - a phenomenon we termed the 'bad apple' effect. Finally, we found that characteristics throughout early and late tumor evolution provide significant and complementary prognostic power in predicting patient survival.
CONCLUSIONS: Taken together, our study generated a comprehensive landscape of evolutionary history for HCC and provides a rich multi-omics resource for understanding tumor heterogeneity and clinical trajectories.
IMPACT AND IMPLICATIONS: This prospective study, utilizing comprehensive multi-sector, multi-omics sequencing and clinical data from surgically resected hepatocellular carcinoma (HCC), reveals critical insights into the role of tumor evolution and intra-tumor heterogeneity (ITH) in determining the prognosis of HCC. These findings are invaluable for oncology researchers and clinicians, as they underscore the influence of distinct evolutionary paths and the 'bad apple' effect, where the most aggressive tumor fraction dictates disease progression. These insights not only enhance prognostic accuracy post-surgical resection but also pave the way for personalized treatment strategies tailored to specific tumor evolutionary and transcriptomic profiles. The coexistence of multiple subtypes within the same tumor prompts a re-appraisal of the utilities of depending on single samples to represent the entire tumor and suggests the need for clinical molecular imaging. This research thus marks a significant step forward in the clinical understanding and management of HCC, underscoring the importance of integrating tumor evolutionary dynamics and multi-omics biomarkers into therapeutic decision-making.
CLINICAL TRIAL NUMBER: NCT03267641 (Observational cohort).