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  1. Asma Ilyani, K., *Rafii, M. Y, Sobri, H., Anna, L. P. K., Rahim, A. H., Mahmud, T. M. M., et al.
    MyJurnal
    The awareness of the general public on healthy foods has been a major concern and people are looking for the right variety of rice for diabetic patients. High amylose content rice with low glycaemic index (GI), which is an indicator of sugar release in the blood, is beneficial for human health. The present work was aimed to determine the physicochemical characteristics and nutritional compositions of MR219 mutant rice, and the effects of amylose content to blood glucose response and glycaemic index in field condition. A total of 31 M4 mutant lines (ML1 to ML31) were evaluated for physicochemical characteristics and nutritional compositions in comparison with the parental variety, MR219. In glycaemic response study, 48 female BALB/c mice were fed with glucose (a baseline), saline water, two check varieties (MR219 and MRQ74) and four selected mutant lines with different amylose contents. The physicochemical and proximate analysis revealed highly significant differences among the mutant lines. Some mutant lines improved amylose content and nutritional composition. Mutant ML3 had slightly higher amylose content than the parental variety and was recommended for glycaemic responses. However, the field experiment results showed two mutant lines namely; ML3 and ML30, having significantly lower glucose reading (5.49 mmol/L and 5.47 mmol/L, respectively) as compared to the parental variety and other mutant lines. The glucose level was found highest at 60 min after feeding but significantly dropped at 120 min. The normal glucose reading in ML3 and ML30 also resulted in moderate GI values (65% and 66%, respectively). As low and moderate GI foods are recommended for diabetic patients, ML3 and ML30 had high potential for their consumption, and can be suggested for further breeding program to develop low GI rice.
  2. Usman MG, Rafii MY, Martini MY, Oladosu Y, Kashiani P
    J Sci Food Agric, 2017 Mar;97(4):1164-1171.
    PMID: 27290898 DOI: 10.1002/jsfa.7843
    BACKGROUND: Studies on genotypic and phenotypic correlations among characters of crop plants are useful in planning, evaluating and setting selection criteria for the desired characters in a breeding program. The present study aimed to estimate the phenotypic correlation coefficients among yield and yield attributed characters and to work out the direct and indirect effects of yield-related characters on yield per plant using path coefficient analysis. Twenty-six genotypes of chili pepper were laid out in a randomized complete block design with three replications.

    RESULTS: Yield per plant showed positive and highly significant (P ≤ 0.01) correlations with most of the characters studied at both the phenotypic and genotypic levels. By contrast, disease incidence and days to flowering showed a significant negative association with yield. Fruit weight and number of fruits exerted positive direct effect on yield and also had a positive and significant (P ≤ 0.01) correlation with yield per plant. However, fruit length showed a low negative direct effect with a strong and positive indirect effect through fruit weight on yield and had a positive and significant association with yield.

    CONCLUSION: Longer fruits, heavy fruits and a high number of fruits are variables that are related to higher yields of chili pepper under tropical conditions and hence could be used as a reliable indicator in indirect selection for yield. © 2016 Society of Chemical Industry.

  3. Oladosu Y, Rafii MY, Abdullah N, Abdul Malek M, Rahim HA, Hussin G, et al.
    ScientificWorldJournal, 2014;2014:190531.
    PMID: 25431777 DOI: 10.1155/2014/190531
    Genetic based knowledge of different vegetative and yield traits play a major role in varietal improvement of rice. Genetic variation gives room for recombinants which are essential for the development of a new variety in any crop. Based on this background, this work was carried out to evaluate genetic diversity of derived mutant lines and establish relationships between their yield and yield components using multivariate analysis. To achieve this objective, two field trials were carried out on 45 mutant rice genotypes to evaluate their growth and yield traits. Data were taken on vegetative traits and yield and its components, while genotypic and phenotypic coefficients, variance components, expected genetic advance, and heritability were calculated. All the genotypes showed variations for vegetative traits and yield and its components. Also, there was positive relationship between the quantitative traits and the final yield with the exception of number of tillers. Finally, the evaluated genotypes were grouped into five major clusters based on the assessed traits with the aid of UPGMA dendrogram. So hybridization of group I with group V or group VI could be used to attain higher heterosis or vigour among the genotypes. Also, this evaluation could be useful in developing reliable selection indices for important agronomic traits in rice.
  4. Ab Halim AAB, Rafii MY, Osman MB, Oladosu Y, Chukwu SC
    Biomed Res Int, 2021;2021:8350136.
    PMID: 34095311 DOI: 10.1155/2021/8350136
    High kernel elongation (HKE) is one of the high-quality characteristics in rice. The objectives of this study were to determine the effects of ageing treatments, gene actions, and inheritance pattern of kernel elongation on cooking quality in two populations of rice and determine the path of influence and contribution of other traits to kernel elongation in rice. Two rice populations derived from crosses between MR219 × Mahsuri Mutan and MR219 × Basmati 370 were used. The breeding materials included two F1 progenies from the two populations, and their respective parents were grown in four different batches at a week interval to synchronize the flowering between the female and male plants. Scaling tests and generation means analysis were carried out to determine ageing effects and estimate additive-dominance gene action and epistasis. The estimation of gene interaction was based on quantitative traits. Path coefficient analysis was done using SAS software version 9.4 to determine the path of influence (direct or indirect) of six quantitative traits on HKE. Results obtained showed that nonallelic gene interaction was observed in all traits. The results before ageing and after ageing showed significant differences in all traits, while the gene interaction changed after ageing. The HKE value improved after ageing, suggesting that ageing is an external factor that could influence gene expression. The epistasis effect for HKE obtained from the cross Mahsuri Mutan × MR219 showed duplicate epistasis while that obtained from a cross between Basmati 370 × MR219 showed complimentary epistasis. Besides, the heritability of HKE was higher in Basmati 370 × MR219 compared to that obtained in Mahsuri Mutan × MR219. The path analysis showed that the cooked grain length and length-width ratio positively significantly affected HKE. It was concluded that ageing treatment is an external factor that could improve the expression of HKE. The findings from this study would be useful to breeders in the selection and development of new specialty (HKE) rice varieties.
  5. Chukwu SC, Ibeji CA, Ogbu C, Oselebe HO, Okporie EO, Rafii MY, et al.
    Sci Rep, 2022 Nov 09;12(1):19054.
    PMID: 36351926 DOI: 10.1038/s41598-022-16833-9
    Mushrooms are fleshy fungi valued globally for their nutritional and medical benefits. The study was conducted at Ebonyi State University Mushroom Center, Abakaliki, to determine an optimum level of limestone (CaCO3) on the genotypes for maximum growth and yield. The experiment was carried out as a split-plot experiment in a completely randomized design (CRD) with the use of Oyster mushroom variety. The two genotypes (GI and GII) were placed in the whole plot while limestone was placed in the sub-plot which consisted of five rates of CaCO3(Og,5 g, 10 g, 15 g and 20 g). Sawdust and rice husk substrates were used at the ratio of 60:40 and sterilized for six hours at 121 °C using the steam sterilization cylinder. The media bags were off-loaded after one day and allowed to further cool for another day before inoculation. The cultured spawn was used to inoculate the media upon cooling at room temperature. Data were collected on agro-morphological parameters such as primordial initiation, stalk height, stalk diameter, number of branches, number of fruits, number of productive bags, fresh and dry weights, and subjected to analysis of variance (ANOVA). The result obtained indicated that there was a significant difference (P  0.05) in all parameters evaluated except the stalk diameter. Genotype I initiated more primordial compared to primordial initiation in genotype II and they differed significantly (p 
  6. Halim AABA, Rafii MY, Osman MB, Chukwu SC, Oladosu Y
    Foods, 2023 May 31;12(11).
    PMID: 37297452 DOI: 10.3390/foods12112207
    Heat treatment could affect the structure and properties of rice varieties. The present study was conducted in order to determine the effects of heat treatment on the physicochemical properties and tissue structure of Mahsuri Mutan, Basmati 370 and MR219 rice varieties. The three rice varieties were subjected to heat treatment (ageing) at 90 °C, using an oven, for 3 h. After the heat treatment, the samples were cooled at room temperature (25 °C) for 1 h. Physicochemical properties, such as alkali digestion value, water uptake ratio, solids in cooking water, high kernel elongation ratio and amylose contents, were determined. The procedure used in determining both apparent and absolute amylose involved measuring the iodine affinity of defatted whole starch. Ahigh-performance anion-exchange chromatograph was used to analyse branch chain length distribution of amylopectin quantitatively. The starch structure of the rice samples was observed under a scanning electron microscope. Data collected on physicochemical traits, heat treatment and control (ageing and non-ageing) were subjected to an analysis of variance using SAS software version 9.4. In this study, Mahsuri Mutan and Basmati 370 showed superior high kernel elongation as compared to their respective rice progenies. This study also found that heat treatment directly affected the increasingly high kernel elongation for both populations. The phenotypic correlation co-efficient indicated that there was a high positive correlation between high kernel elongation and water uptake ratio, implying that selection for water uptake ratio would increase the high kernel elongation characteristic. The heat treatment showed significant difference in all the physicochemical traits of the varieties studied. Heat treatment also affected the very long branch chains of starch, such as amylose. Observation under an electron microscope showed that the samples subjected to heat treatment had more cracks on the tissue structure compared to normal rice samples. The hexagon structure in Mahsuri Mutan produced a greater elongation effect on its kernel. The findings from this study could be useful to breeders in the selection and development of a new high kernel elongation rice variety.
  7. Oladosu Y, Rafii MY, Abdullah N, Magaji U, Hussin G, Ramli A, et al.
    Biomed Res Int, 2016;2016:7985167.
    PMID: 27429981 DOI: 10.1155/2016/7985167
    Rice cultivation generates large amount of crop residues of which only 20% are utilized for industrial and domestic purposes. In most developing countries especially southeast Asia, rice straw is used as part of feeding ingredients for the ruminants. However, due to its low protein content and high level of lignin and silica, there is limitation to its digestibility and nutritional value. To utilize this crop residue judiciously, there is a need for improvement of its nutritive value to promote its utilization through ensiling. Understanding the fundamental principle of ensiling is a prerequisite for successful silage product. Prominent factors influencing quality of silage product include water soluble carbohydrates, natural microbial population, and harvesting conditions of the forage. Additives are used to control the fermentation processes to enhance nutrient recovery and improve silage stability. This review emphasizes some practical aspects of silage processing and the use of additives for improvement of fermentation quality of rice straw.
  8. Sahmat SS, Rafii MY, Oladosu Y, Jusoh M, Hakiman M, Mohidin H
    Sci Rep, 2024 Jan 19;14(1):1698.
    PMID: 38242885 DOI: 10.1038/s41598-023-50381-0
    Evaluation of genotypes to identify high-yielding and stable varieties is crucial for chilli production sustainability and food security. These analyses are essential, particularly when the breeding program aims to select lines with great adaptability and stability. Thirty chilli genotypes were evaluated for yield stability under four soilless planting systems viz; fertigation, HydroStock (commercial hydrogel), BioHydrogel (biodegradable hydrogel), and hydroponic to study the influence of genotype by environment interaction. The research used a split-plot randomized complete block design (RCBD) with two cropping cycles and five replications. The GGE biplot analysis was employed to assess the mean versus stability perspective in explaining the variation in genotypic and genotype-by-environment effects on the yield-related attributes for yield per plant, fruit number, fruit length, and width. Stability analysis denoted genotypes G26 and G30 as the most stable for yield per plant, while G16, G22, and G30 were stable for the number of fruits per plant. Among the four planting systems evaluated, HydroStock and BioHydrogel outperformed the others in yield per plant, demonstrating the highest level of informativeness or discrimination. These findings offer critical insights for future crop breeding programs and the optimization of agricultural practices.
  9. Chukwu SC, Rafii MY, Ramlee SI, Ismail SI, Hasan MM, Oladosu YA, et al.
    Mol Biol Rep, 2019 Feb;46(1):1519-1532.
    PMID: 30628024 DOI: 10.1007/s11033-019-04584-2
    Breeding for disease resistant varieties remains very effective and economical in controlling the bacterial leaf blight (BLB) of rice. Breeders have played a major role in developing resistant rice varieties against the BLB infection which has been adjudged to be a major disease causing significant yield reduction in rice. It would be difficult to select rice crops with multiple genes of resistance using the conventional approach alone. This is due to masking effect of genes including epistasis. In addition, conventional breeding takes a lot of time before a gene of interest can be introgressed. Linkage drag is also a major challenge in conventional approach. Molecular breeding involving markers has facilitated the characterization and introgression of BLB disease resistance genes. Biotechnology has brought another innovation in form of genetic engineering (transgenesis) of rice. Although, molecular breeding cannot be taken as a substitute for conventional breeding, molecular approach for combating BLB disease in rice is worthwhile given the demand for increased production of rice in a fast growing population of our society. This present article highlights the recent progress from conventional to molecular approach in breeding for BLB disease resistant rice varieties.
  10. Oladosu Y, Rafii MY, Arolu F, Chukwu SC, Salisu MA, Olaniyan BA, et al.
    Plants (Basel), 2021 Aug 20;10(8).
    PMID: 34451758 DOI: 10.3390/plants10081714
    Eggplant is the fifth economically most important vegetable in the Solanaceae family after tomato, potato, chili, and tobacco. Apart from the well-cultivated brinjal or aubergine eggplant (Solanum melongena L.), two other underutilized eggplant species, the African eggplant (S. macrocarpon L.) and the scarlet eggplant (S. aethiopicum L.), were also cultivated with local importance where the leaves and fruits are used for food and medicinal purposes. The major objectives of the eggplant breeding program are to improve fruit quality, increase yield performance through heterosis breeding, and introduce pest and disease resistances from wild relatives. Europe and Asia hold a wide collection of germplasm resources with significant potential for genetic improvement. While cultivated eggplant is susceptible to several fungi and bacteria, many wild relatives offer potential resistance to these pathogens. In this paper, we review the genetic resources and diversity of cultivated eggplant and its wild relatives. As a point of departure, we examine the economic importance, domestication, taxonomy characterization, and relationships of the crop and its wild relatives. The importance of evaluating and safeguarding wild relatives is highlighted, as crop wild relatives are highly underrepresented. A key section in this study is an overview dedicated to genetic resources, resistance to biotic and abiotic stresses, pre-breeding, and breeding for sustainable eggplant production.
  11. Oladosu Y, Rafii MY, Magaji U, Abdullah N, Miah G, Chukwu SC, et al.
    Biomed Res Int, 2018;2018:8936767.
    PMID: 30105259 DOI: 10.1155/2018/8936767
    The associations among yield-related traits and the pattern of influence on rice grain yield were investigated. This evaluation is important to determine the direct and indirect effects of various traits on yield to determine selection criteria for higher grain yield. Fifteen rice genotypes were evaluated under tropical condition at five locations in two planting seasons. The experiment was laid out in a randomized complete block design with three replications across the locations. Data were collected on vegetative and yield components traits. The pooled data based on the analysis of variance revealed that there were significant differences (p < 0.001) among the fifteen genotypes for all the characters studied except for panicle length and 100-grain weight. Highly significant and positive correlations at phenotypic level were observed in grain weight per hill (0.796), filled grains per panicle (0.702), panicles per hill (0.632), and tillers per hill (0.712) with yield per hectare, while moderate positive correlations were observed in flag leaf length to width ratio (0.348), days to flowering (0.412), and days to maturity (0.544). By contrast, unfilled grains per panicle (-0.225) and plant height (-0.342) had a negative significant association with yield per hectare. Filled grains per panicle (0.491) exhibited the maximum positive direct effect on yield followed by grain weight per hill (0.449), while unfilled grain per panicle (-0.144) had a negative direct effect. The maximum indirect effect on yield per hectare was recorded by the tillers per hill through the panicles per hill. Therefore, tillers per hill, filled grains per panicle, and grain weight per hill could be used as selection criteria for improving grain yield in rice.
  12. Oladosu Y, Rafii MY, Samuel C, Fatai A, Magaji U, Kareem I, et al.
    Int J Mol Sci, 2019 Jul 18;20(14).
    PMID: 31323764 DOI: 10.3390/ijms20143519
    Drought is the leading threat to agricultural food production, especially in the cultivation of rice, a semi-aquatic plant. Drought tolerance is a complex quantitative trait with a complicated phenotype that affects different developmental stages in plants. The level of susceptibility or tolerance of rice to several drought conditions is coordinated by the action of different drought-responsive genes in relation with other stress components which stimulate signal transduction pathways. Interdisciplinary researchers have broken the complex mechanism of plant tolerance using various methods such as genetic engineering or marker-assisted selection to develop a new cultivar with improved drought resistance. The main objectives of this review were to highlight the current method of developing a durable drought-resistant rice variety through conventional breeding and the use of biotechnological tools and to comprehensively review the available information on drought-resistant genes, QTL analysis, gene transformation and marker-assisted selection. The response, indicators, causes, and adaptation processes to the drought stress were discussed in the review. Overall, this review provides a systemic glimpse of breeding methods from conventional to the latest innovation in molecular development of drought-tolerant rice variety. This information could serve as guidance for researchers and rice breeders.
  13. Swaray S, Y Rafii M, Din Amiruddin M, Firdaus Ismail M, Jamian S, Jalloh M, et al.
    Insects, 2021 Mar 04;12(3).
    PMID: 33806613 DOI: 10.3390/insects12030221
    This study was conducted to assess the Elaeidobius kamerunicus (EK) population density among the biparental dura × pisifera hybrids' palms on deep peat-soil. Twenty-four hybrids derived from 10 genetic sources were used. Variance analysis showed that the EK population density varies between different oil palm hybrids, with a more noticeable variation of a low population mean in the male weevil across the hybrids. The highest weevil population mean/spikelet was attained on the third day of anthesis. The maximum monthly population of EK/spikelet (12.81 ± 0.23) and population density of EK (1846.49 ± 60.69) were recorded in January. Accordingly, 41.67% of the hybrids recorded an EK population density greater than the trial means of 973.68 weevils. Hybrid ECPHP550 had the highest mean of EK/spikelet (10.25 ± 0.11) and the highest population density of EK/palm (1241.39 ± 73.74). The parental mean population was 963.24 weevils and parent Deli-Banting × AVROS recorded the highest EK population density (1173.01). The overall results showed a notable disparity in the EK population among the biparental hybrids. Parental Deli-Banting × AVROS and hybrid ECPHP550 could be more useful to optimize the weevil population for pollination improvements in palm plantations. However, we suggest that volatile production should be included as a desirable trait in oil palm selective breeding.
  14. Musa I, Rafii MY, Ahmad K, Ramlee SI, Md Hatta MA, Oladosu Y, et al.
    Plants (Basel), 2020 Nov 15;9(11).
    PMID: 33203189 DOI: 10.3390/plants9111583
    Grafting is regarded as an integral component of sustainable vegetable production. It is important in the management of soil-borne diseases, and reports suggest that grafting with viable rootstocks can enhance crop growth and yield. This research was conducted using splices and cleft grafting techniques to investigate graft compatibility among varieties of high yielding eggplant scion (MCV1, MCV2, CCV1, CCV2, CCV3, NCV, and TCV) grafted onto wild rootstocks (MWR, BWR, and TWR) to study their morphophysiological and yield characteristics. High yielding scions grafted onto wild relative rootstocks were compared with two controls including self-grafted and non-grafted. All the scion had a high rate of germination (≥95%) and remarkable graft success (100%) was recorded in MCV1, MCV2, and TCV using the cleft techniques. Generally, the use of rootstocks resulted in higher total and marketable fruit yield compared to the non-grafted and self-grafted scion plants, respectively. In particular, MWR and TWR rootstock conferred the highest vigour to the scion, resulting in the highest values recorded for total and marketable fruit yield, number of fruits per plant and average fruit weight. A similar result was obtained in fruit length and diameter, where long and wide fruits were observed in scions grafted onto MWR and TWR rootstocks, respectively. Grafting of high yielding eggplant scion onto resistant MWR, BWR and TWR eggplant rootstock was found to be beneficial for eggplant cultivation. The remarkable compatibility and vigour of the rootstock with scion led to the improvement in total and marketable yield of the fruits. As such, it can be concluded that the use of wild relative rootstocks of eggplant species can be a valuable method of improving eggplant production.
  15. Kayode FI, Taiwo IE, Adeogun AO, Olalekan O, Chimdalu IP, Olayilola OI, et al.
    Afr Health Sci, 2023 Mar;23(1):255-261.
    PMID: 37545927 DOI: 10.4314/ahs.v23i1.27
    OBJECTIVE: We evaluated the susceptibility status of Anopheles gambiae in two communities of Ikorodu, Lagos, Nigeria to DDT, deltamethrin, lambda cyhalothrin and bendiocarb.

    METHODS: Anopheles immature stages were collected from their habitats in the surveyed community and allowed to emerge before exposure adult females to discriminating doses of WHO insecticides including DDT, deltamethrin, lambda cyhalothrin, bendiocarb and malathion. PBO synergistic bioassay was conducted for insecticides where the mosquito samples showed resistance. PCR assay was used for the detection of kdr mutation in the mosquitoes.

    RESULTS: Resistance to DDT (40% and 86%) and lambda cyhalothrin (75% and 84%) in Oke-Ota and Majidun respectively. Suspected resistance to deltamethrin (94.9%) and bendiocarb (93.5%) was recorded in Oke-Ota community and the mosquitoes were susceptible to malathion in both communities. KDR mutation (L1014F) from resistance samples from both locations though with a low frequency that significantly departs from Hardy-Weinberg's probability (P> 0.01). PBO synergized bioassay was able to increase knockdown, percentage mortality and restore full susceptibility to deltamethrin and bendiocarb.

    CONCLUSION: Results from this study indicates that the metabolic resistance mechanism is highly implicated in the resistance to different classes of insecticide in Ikorodu and this should be taken into consideration when implementing vector control activities in this area.

  16. Myint KA, Yaakub Z, Rafii MY, Oladosu Y, Samad MYA, Ramlee SI, et al.
    Biomed Res Int, 2021;2021:6620645.
    PMID: 33997027 DOI: 10.1155/2021/6620645
    Molecular characterization of oil palm germplasm is crucial in utilizing and conserving germplasm with promising traits. This study was conducted to evaluate the genetic diversity structures and relationships among 26 families of MPOB-Senegal oil palm germplasm using thirty-five microsatellite markers. High level of polymorphism (P = 96.26%), number of effective allele (N e = 2.653), observed heterozygosity (H o = 0.584), expected heterozygosity (H e = 0.550), total heterozygosity (H T = 0.666), and rare alleles (54) were observed which indicates that MPOB-Senegal germplasm has a broad genetic variation. Among the SSR markers, sMo00053 and sMg00133 were the most informative markers for discrimination among the MPOB-Senegal oil palm germplasm for having the highest private alleles and the rare alleles. For selection and conservation, oil palm populations with high rare alleles and Nei's gene diversity index should be considered as these populations may possess unique genes for further exploitation.
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