MyMedR

Raised leptin concentrations in feto-placental tissues from women with preeclampsia

Singh HJ, Abu Bakar A, Che Romli A, Nila A

Hypertens Pregnancy, 2005;24(2):191-9.

The aim of this study was to estimate the levels of leptin in the amnion, chorion laeve, and placenta and to examine for any differences in leptin levels in these tissues from preeclamptic and normotensive pregnant women.

Matched MeSH terms: Amniotic Fluid/chemistry*

Sudden maternal deaths from amniotic fluid embolism

Nadesan K, Jayalakshmi P

Ceylon Med J, 1997 Dec;42(4):185-9.

PMID: 9476404

Sudden maternal death from amniotic fluid embolism is a rare but serious complication which usually occurs during late pregnancy, often during labour or shortly after, with more than 80% mortality. Such a death causes immense stress to both the relatives and the attending doctors as it is sudden and unexpected. Three such deaths are discussed here. Traditionally, it was believed that this complication usually occurred in prolonged and difficult labour, but there is evidence contrary to this, and it is not possible to predict when and where this fatal complication will occur. Current views and various pathophysiologic mechanisms leading to the fatal outcome are also discussed.

Matched MeSH terms: Embolism, Amniotic Fluid/diagnosis*

TRENDS IN OBSTETRICS

MACAFEE CH

Med J Malaysia, 1963 Jun;17:274-81.

PMID: 14060504

Matched MeSH terms: Amniotic Fluid*; Embolism, Amniotic Fluid*

Fulltext Searching for the Origin through Central Nervous System: A Review and Thought which Related to Microgravity, Evolution, Big Bang Theory and Universes, Soul and Brainwaves, Greater Limbic System and Seat of the Soul

Idris Z

Malays J Med Sci, 2014 Jul;21(4):4-11.

PMID: 25977615 MyJurnal

Cerebrospinal fluid (CSF) serves buoyancy. The buoyancy thought to play crucial role in many aspects of the central nervous system (CNS). Weightlessness is produced mainly by the CSF. This manuscript is purposely made to discuss its significance which thought contributing towards an ideal environment for the CNS to develop and function normally. The idea of microgravity environment for the CNS is supported not only by the weightlessness concept of the brain, but also the noted anatomical position of the CNS. The CNS is positioned in bowing position (at main cephalic flexure) which is nearly similar to an astronaut in a microgravity chamber, fetus in the amniotic fluid at early gestation, and animals and plants in the ocean or on the land. Therefore, this microgravity position can bring us closer to the concept of origin. The hypothesis on 'the origin' based on the microgravity were explored and their similarities were identified including the brainwaves and soul. Subsequently a review on soul was made. Interestingly, an idea from Leonardo da Vinci seems in agreement with the notion of seat of the soul at the greater limbic system which has a distinctive feature of "from God back to God".

Matched MeSH terms: Amniotic Fluid

Prospective full-term-derived pluripotent amniotic fluid stem (AFS) cells

Ferdaos N, Nathan S, Nordin N

Med J Malaysia, 2008 Jul;63 Suppl A:75-6.

PMID: 19024991

Amniotic fluid (AF) serves as an excellent alternative source of pluripotent stem cells, as they are not bound with ethical issues and the stem cells are more primitive than adult stem (AS) cells. Hence, they have higher potential. Here we aim to isolate and characterize pluripotent stem cells from mid-term and full-term pregnant rat amniotic fluid. The results demonstrate the evidence of heterogeneous population of cells in the amniotic fluid and some of the cells morphology shows similarity with ES cells.

Matched MeSH terms: Amniotic Fluid/physiology*

Isolation of human surfactant protein A from amniotic fluid

Cheong KB, Cheong SK, Boo NY, Jemilah M, Ton SH

Malays J Pathol, 1995 Dec;17(2):97-101.

PMID: 8935134

Surfactant protein A (SP-A) is one of the four known surfactant-associated proteins found in human lungs. It plays a major role in determining regulation of surfactant uptake and resecretion. Qualitative and quantitative deficiencies of SP-A may contribute to neonatal respiratory distress syndrome. The measurement of its level in amniotic fluid or neonatal tracheal aspirate may be useful in the assessment of replacement therapy using natural or synthetic surfactants. In order to develop an in-house immunoassay to detect the level of SP-A, we used a discontinuous sucrose density gradient to isolate SP-A from amniotic fluid. Polyacrylamide gel electrophoresis was carried out on the isolates with low molecular weight markers. We successfully isolated SP-A from 12 out of 31 samples of amniotic fluid. The isolates were found to be relatively pure and have a molecular weight of about 35 kD. The isolated SP-A were used as immunogens to raise antibodies in rabbits for the immunoassay.

Matched MeSH terms: Amniotic Fluid/chemistry*

Rat full term amniotic fluid harbors highly potent stem cells

Mun-Fun H, Ferdaos N, Hamzah SN, Ridzuan N, Hisham NA, Abdullah S, et al.

Res Vet Sci, 2015 Oct;102:89-99.

PMID: 26412526 DOI: 10.1016/j.rvsc.2015.07.010

Amniotic fluid stem cells (AFSCs) are commonly isolated from mid-term amniotic fluid (AF) of animals and human collected via an invasive technique, amniocentesis. Alternatively, AFSCs could be collected at full-term. However, it is unclear whether AFSCs are present in the AF at full term. Here, we aimed to isolate and characterize stem cells isolated from AF of full term pregnant rats. Three stem cell lines have been established following immuno-selection against the stem cell marker, c-kit. Two of the new lines expressed multiple markers of pluripotency until more than passage 90. Further, they spontaneously differentiated into derivatives of the three primary germ layers through the formation of good quality embryoid bodies (EBs), and can be directly differentiated into neural lineage. Their strong stemness and potent neurogenic properties highlight the presence of highly potent stem cells in AF of full-term pregnancies, which could serve as a potential source of stem cells for regenerative medicine.

Matched MeSH terms: Amniotic Fluid/cytology*

Stem Cells Derived from Amniotic Fluid: A Potential Pluripotent-Like Cell Source for Cellular Therapy?

Ramasamy TS, Velaithan V, Yeow Y, Sarkar FH

Curr Stem Cell Res Ther, 2018;13(4):252-264.

PMID: 29336267 DOI: 10.2174/1574888X13666180115093800

BACKGROUND: Regenerative medicine aims to provide therapeutic treatment for disease or injury, and cell-based therapy is a newer therapeutic approach different from conventional medicine. Ethical issues that rose by the utilisation of human embryonic stem cells (hESC) and the limited capacity of adult stem cells, however, hinder the application of these stem cells in regenerative medicine. Recently, isolation and characterisation of c-kit positive cells from human amniotic fluid, which possess intermediate characteristics between hESCs and adult stem cells, provided a new approach towards realising their promise for fetal and adult regenerative medicine. Despite the number of studies that have been initiated to characterize their molecular signature, research on developing approaches to maintain and enhance their regenerative potential is urgently needed and must be developed.

AIM: Thus, this review is focused on understanding their potential uses and factors influencing their pluripotent status in vitro.

CONCLUSION: In short, this cell source could be an ideal cellular resource for pluripotent cells for potential applications in allogeneic cellular replacement therapies, fetal tissue engineering, pharmaceutical screening, and in disease modelling.

Matched MeSH terms: Amniotic Fluid/cytology*

A plethora of human pluripotent stem cells

Gao L, Thilakavathy K, Nordin N

Cell Biol Int, 2013 Sep;37(9):875-87.

PMID: 23619972 DOI: 10.1002/cbin.10120

At the early stages of mammalian development, a number of developmentally plastic cells appear that possess the ability to give rise to all of the differentiated cell types normally derived from the three primary germ layers - unique character known as pluripotency. To date, embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have been shown to be truly pluripotent. However, recent studies have revealed a variety of other cells that demonstrate pluripotentiality, including very small embryonic-like stem cells (VSELs), amniotic fluid stem cells (AFSCs), marrow-isolated adult multilineage inducible cells (MIAMI) and multipotent adult precursor cells (MAPCs). This review summarises key features of these six kinds of pluripotent and potentially pluripotent stem cells (ESCs, iPSCs, VSELs, AFSCs, MIAMI and MAPCs) and the evidence for their pluripotency properties.

Matched MeSH terms: Amniotic Fluid/cytology*; Amniotic Fluid/metabolism

Fulltext Sudden maternal deaths probably due to obstetrical pulmonary embolism in Malaysia for 1991

Ravindran J

Med J Malaysia, 1994 Mar;49(1):53-61.

PMID: 8057992

Obstetric pulmonary embolism forms the most serious vascular problem during pregnancy. The following report is a retrospective study into maternal deaths in Malaysian mothers probably due to obstetrical pulmonary embolism. Obstetric pulmonary embolism can be divided into amniotic fluid embolism and obstetric blood clot embolism. There were 37 maternal deaths attributable to this cause -9.9 per cent of all maternal deaths to blood clot embolism and 6.7 per cent to amniotic fluid embolism. Most cases were diagnosed clinically because a postmortem examination was not done. Eleven cases of embolism were associated with Caesarean delivery. The typical profile of a Malaysian mother dying from obstetrical pulmonary embolism is that of a Malay mother in the "non-risk" parities of one to four and usually aged between 31-35 years. In the management of these patients, there should be an urgency in diagnosis and improvement in diagnostic procedures. Health staff at all levels should be trained to pick up patients who present with features of deep vein thrombosis.

Matched MeSH terms: Embolism, Amniotic Fluid/diagnosis; Embolism, Amniotic Fluid/mortality*; Embolism, Amniotic Fluid/prevention & control

Accelerated wound closure - Systematic evaluation of cellulose acetate effects on biologically active molecules release from amniotic fluid stem cells (AFSCs)

Nuge T, Liu X, Tshai KY, Lim SS, Nordin N, Hoque ME, et al.

Biotechnol Appl Biochem, 2021 Apr 07.

PMID: 33826152 DOI: 10.1002/bab.2162

Despite a lot of intensive research on cells-scaffolds interaction, focused are mainly on the capacity of construct scaffolds to regulate cell mobility, migration and cytotoxicity. The effect of the scaffold's topographical and material properties on the expression of biologically active compounds from stem cells is not well understood. In this study, the influence of cellulose acetate (CA) on the electrospinnability of gelatin and the roles of gelatin-cellulose acetate (Ge-CA) on modulating the release of biologically active compounds from amniotic fluid stem cells (AFSCs) is emphasized. It was found that the presence of a small amount of CA could provide a better microenvironment that mimics AFSCs' niche. However, a large amount of CA exhibited no significant effect on AFSCs migration and infiltration. Further study on the effect of surface topography and mechanical properties on AFSCs showed that the tailored microenvironment provided by the Ge-CA scaffolds had transduced physical cues to biomolecules released into the culture media. It was found that the AFSCs seeded on electrospun scaffolds with less CA proportions has profound effects on the secretion of metabolic compounds compared to those with higher CA contained and gelatin coating. The enhanced secretion of biologically active molecules by the AFSCs on the electrospun scaffolds was proven by the accelerated wound closure on the injured human dermal fibroblast (HDF) model. The rapid HDF cell migration could be anticipated due to a higher level of paracrine factors in AFSCs media. Our study demonstrates that the fibrous topography and mechanical properties of the scaffold is a key material property that modulates the high expression of biologically active compounds from the AFSCs. The discovery elucidates a new aspect of material functions and scaffolds material-AFSCs interaction for regulating biomolecules release to promote tissue regeneration/repair. To the best of our knowledge, this is the first report describing the scaffolds material-AFSCs interaction and the efficacy of scratch assays on quantifying the cell migration in response to the AFSCs metabolic products. This article is protected by copyright. All rights reserved.

Matched MeSH terms: Amniotic Fluid

Fulltext Evaluation of two cell culture media in culturing rat full term amniotic fluid cells

Ferdaos, N., Karuppiah, T., Rosli, R., Yazid, M. N., Nordin, N.

Malaysian Journal of Medicine and Health Sciences, 2011;7(2):81-85.

MyJurnal

Introduction: Amniotic fluid (AF) consists of heterogenous population of cells with high diagnostic
and therapeutic values. The study of rat amniotic fluid cells is very limited, despite the extensive use
of this animal model in biomedical research. Primary culture of rat AF cells, especially from full term pregnancies has not been well established. Here we attempt to determine the suitable medium in
culturing rat AF cells that would enhance the cell viability, growth rate and heterogeneity. Methods:
The cell viability, growth rate and heterogeneity of rat AF cells were compared upon culturing the
primary cells in two different media; Amniomax or RPMI. Cell viability study was carried out using
trypan blue staining, while the growth rate was monitored based on the time required to passage the cells (population doubling time in hour). The heterogeneity of cells was examined based on the morphology of the cells. Statistical analysis was performed using t-test. Results: Amniomax was observed to provide a better culture condition in culturing rat AF cells as the cells are more viable, grow faster and more heterogenous as compared to the cells grown in RPMI. Conclusion: Amniomax is a more suitable medium for high quality and viability of full term rat AF cell culture, as compared to RPMI. Thus, warranting propagation of more rat AF cells for biomedical research.

Matched MeSH terms: Amniotic Fluid

Fulltext Isoflavone genistein induces fluid secretion and morphological changes in the uteri of post-pubertal rats

Salleh N, Helmy MM, Fadila KN, Yeong SO

Int J Med Sci, 2013;10(6):665-75.

PMID: 23569430 DOI: 10.7150/ijms.5207

A reported increase in the incidence of infertility following high genistein intake could be related to alteration in the normal fluid volume and morphology of the uterus in adult female. In view of this, we investigated the effect of this compound on fluid secretion, fluid volume and morphology of the uterus in post-pubertal rats.

Matched MeSH terms: Amniotic Fluid/drug effects

Ultrasound and clinical predictors for Caesarean delivery after labour induction at term

Tan PC, Suguna S, Vallikkannu N, Hassan J

Aust N Z J Obstet Gynaecol, 2006 Dec;46(6):505-9.

PMID: 17116055

To assess the relationship of ultrasound assessment for amniotic fluid, fetal weight, cervical length, cervical funneling and clinical factors on the risk of Caesarean delivery after labour induction at term.

Matched MeSH terms: Amniotic Fluid/ultrasonography

Highly potent stem cells from full-term amniotic fluid: A realistic perspective

Hamid AA, Joharry MK, Mun-Fun H, Hamzah SN, Rejali Z, Yazid MN, et al.

Reprod Biol, 2017 Mar;17(1):9-18.

PMID: 28262444 DOI: 10.1016/j.repbio.2017.02.001

Amniotic fluid (AF) is now known to harbor highly potent stem cells, making it an excellent source for cell therapy. However, most of the stem cells isolated are from AF of mid-term pregnancies in which the collection procedure involves an invasive technique termed amniocentesis. This has limited the access in getting the fluid as the technique imposes certain level of risks to the mother as well as to the fetus. Alternatively, getting AF from full-term pregnancies or during deliveries would be a better resolution. Unfortunately, very few studies have isolated stem cells from AF at this stage of gestation, the fluid that is merely discarded. The question remains whether full-term AF harbors stem cells of similar potency as of the stem cells of mid-term AF. Here, we aim to review the prospect of having this type of stem cells by first looking at the origin and contents of AF particularly during different gestation period. We will then discuss the possibility that the AF, at full term, contains a population of highly potent stem cells. These stem cells are distinct from, and probably more potent than the AF mesenchymal stem cells (AF-MSCs) isolated from full-term AF. By comparing the studies on stem cells isolated from mid-term versus full-term AF from various species, we intend to address the prospect of having highly potent amniotic fluid stem cells from AF of full-term pregnancies in human and animals.

Matched MeSH terms: Amniotic Fluid/cytology*

Fulltext Predictors of newborn admission after labour induction at term: Bishop score, pre-induction ultrasonography and clinical risk factors

Tan PC, Suguna S, Vallikkannu N, Hassan J

Singapore Med J, 2008 Mar;49(3):193-8.

PMID: 18362999

Following labour induction at term, 12 percent of neonates can expected to be admitted to the neonatal intensive care unit. We aimed to evaluate the Bishop score, pre-induction ultrasonography (US) assessment of amniotic fluid, foetal weight and cervical length, and pre-induction and intrapartum risk factors as predictors of neonatal admission.

Matched MeSH terms: Amniotic Fluid/ultrasonography

Fulltext Triplex PCR using new primers for the detection of Toxoplasma gondii

Rahumatullah A, Khoo BY, Noordin R

Exp Parasitol, 2012 Jun;131(2):231-8.

PMID: 22561042 DOI: 10.1016/j.exppara.2012.04.009

Molecular methods are used increasingly for the detection of Toxoplasma gondii infection. This study developed a rapid, sensitive, and specific conventional triplex PCR for the detection of the B1 gene and ITS1 region of T. gondii using newly designed primers and an internal control based on the Vibrio cholerae HemM gene. The annealing temperature and concentrations of the primers, MgCl(2), and dNTPs were optimized. Two sets of primers (set 1 and 2) were tested, which contained different segments of the T. gondii B1 gene, 529 repeat region and ITS1 region. A series of sensitivity tests were performed using parasite DNA, whole parasites, and spiked human body fluids. Specificity tests were performed using DNA from common protozoa and bacteria. The newly developed assay based on set 2 primers was found to be specific and sensitive. The test was capable of detecting as little as 10 pg T. gondii DNA, 10(4) tachyzoites in spiked body fluids, and T. gondii DNA in the organ tissues of experimentally infected mice. The assay developed in this study will be useful for the laboratory detection of T. gondii infection.

Matched MeSH terms: Amniotic Fluid/parasitology

Sudden maternal deaths in Malaysia: a case report

Jegasothy R

J Obstet Gynaecol Res, 2002 Aug;28(4):186-93.

PMID: 12452259

We report on a retrospective study of maternal deaths in Malaysia that occurred within 24 hours of delivery, abortion or operative termination of the pregnancy (defined as sudden deaths) in the years 1995-1996. There were 131 sudden maternal deaths (20.6% of all maternal deaths); postpartum hemorrhage, obstetric embolisms, trauma and hypertensive disorders of pregnancy were the main causes. There was a disproportionately increased risk of sudden maternal deaths in the Chinese and the 'other bumiputra' racial groups. The proportion of mothers who had no obstetric risk factors in the pregnancy that led to death was 16.8%. Fourteen mothers died in transit Twenty mothers died after a cesarean section. The findings of this review emphasize the fact that caregivers in obstetrics need to be forever vigilant. All maternity staff need to be well trained in emergency care and there needs to be quick referral to centers that can provide expertise in handling these emergencies.

Matched MeSH terms: Embolism, Amniotic Fluid/mortality

Onset of labour

Chong HL, Ong HC, Ang LT

Med J Malaysia, 1974 Sep;29(1):44-53.

PMID: 4282630

Matched MeSH terms: Amniotic Fluid/physiology

Introducing external cephalic version in a Malaysian setting

Yong SP

Hong Kong Med J, 2007 Feb;13(1):40-5.

PMID: 17277391

To assess the outcome of external cephalic version for routine management of malpresenting foetuses at term.

Matched MeSH terms: Amniotic Fluid

Displaying publications 1 - 20 of 23 in total