Browse publications by year: 2012

  1. Hartono JL, Mahadeva S, Goh KL
    J Dig Dis, 2012 May;13(5):252-7.
    PMID: 22500787 DOI: 10.1111/j.1751-2980.2012.00581.x
    To examine the differences in the prevalence and severity of anxiety and depression in patients with functional dyspepsia (FD), nonerosive reflux disease (NERD), irritable bowel syndrome (IBS) and healthy controls.
    MeSH terms: Adolescent; Adult; Aged; Anxiety/complications*; Depression/complications*; Dyspepsia/psychology*; Esophagitis, Peptic/psychology*; Female; Humans; Male; Middle Aged; Psychiatric Status Rating Scales; Surveys and Questionnaires; Severity of Illness Index; Prevalence; Irritable Bowel Syndrome/psychology*; Young Adult
  2. Farooqui M, Hassali MA, Abdul Shatar AK, Shafie AA, Seang TB, Farooqui MA
    Complement Ther Clin Pract, 2012 May;18(2):114-20.
    PMID: 22500849 DOI: 10.1016/j.ctcp.2011.09.003
    The current study sought to evaluate Malaysian oncology patients' decision making about the use of Complementary and Alternative Medicine (CAM) for the management of their care. Patients were interviewed across three major Malaysian ethnic groups, Malay, Chinese and Indian. Thematic content analysis identified four central themes: Conceptualizing CAM, the decision making process; rationale given for selecting or rejecting CAM and barriers to CAM use. Participants generally used the term 'traditional medicine', referred to locally as 'ubat kampung', meaning medicine derived from 'local traditions'. Mixed reactions were shown concerning the effectiveness of CAM to cure cancer and the slow progression of CAM results and treatment costs were cited as major barriers to CAM use. Concerns regarding safety and efficacy of CAM in ameliorating cancer as well as potential interactions with conventional therapies highlighted the importance of patients' knowledge about cancer treatments.
    MeSH terms: Adult; Aged; Complementary Therapies/adverse effects; Complementary Therapies/methods; Complementary Therapies/utilization*; Decision Making*; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Medicine, East Asian Traditional/adverse effects; Medicine, East Asian Traditional/methods; Middle Aged; Neoplasms/ethnology; Neoplasms/therapy*; Young Adult
  3. Al Dubai SA, Ganasegeran K, Alabsi AM, Alshagga MA, Ali RS
    Asian Pac J Cancer Prev, 2012;13(1):165-8.
    PMID: 22502661
    OBJECTIVES: This study aimed to assess the level of knowledge of oral cancer and its associated factors among university students in Malaysia.

    METHODS: A cross sectional study was conducted among 200 university students in Malaysia. A self administered questionnaire was used to collect data. It included questions on socio- demographic data, awareness and knowledge of oral cancer.

    RESULTS: Mean age of the respondents was 21.5 ± 2.5 and the age ranged from 18 to 27 years. The majority of the respondents were aware of oral cancer (92.0%) and recognized the followings as signs and symptoms of oral cancer: ulcer and oral bleeding (71.0%), followed by swelling (61.5%). A satisfactory knowledge was observed on the following risk factors; smoking (95.5%), poor oral hygiene (90.5%), family history (90.0%), alcohol (84.5%) and poor fitting dentures (83.0%). However, unsatisfactory knowledge was observed about hot/spicy food (46.5%), obesity (36.0%), old age (31.5%), dietary factor (29.0%) and smokeless tobacco (25.5%). Knowledge of oral cancer was associated significantly with age (p<0.01), year of study (p<0.01) and course of study (p<0.01).

    CONCLUSION: Instead of satisfactory awareness and knowledge of oral cancer and its clinical presentations, inadequate knowledge was observed about its risk factors. There is a need to introduce oral cancer education among university students.
    MeSH terms: Adolescent; Adult; Awareness*; Cross-Sectional Studies; Educational Status; Female; Health Education*; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Mouth Neoplasms/epidemiology; Mouth Neoplasms/prevention & control*; Mouth Neoplasms/psychology; Prognosis; Surveys and Questionnaires; Risk Factors; Students/statistics & numerical data*; Universities; Young Adult
  4. Su TT, Sallehuddin BA, Murniati HH, Swinder J, Al Sadat N, Saimy I
    Asian Pac J Cancer Prev, 2012;13(1):175-9.
    PMID: 22502663
    The objective of the study is to investigate the success rate of quit attempts and identify factors associated with success or failure of quit attempts in a quit smoking clinic. A cohort study was conducted with 495 smokers who enrolled in a quit smoking clinic from 2005 to 2008. The factors leading to quit smoking successfully were "being Malay", "having high blood pressure" "type of Nicotine Replacement Therapy" and "duration of follow up". In contrast, clerical staff had negative association to quit smoking. People who started smoking in their teenage years had a high risk of relapse. Integration of active follow up and tailor-made support programmes for quitters appear necessary in order to maintain their non-smoking status and encourage them to be permanent quitters. Integration of quit smoking clinics and primary care clinics could be another potential step for the success of quit smoking programmes.
    MeSH terms: Adolescent; Adult; Age Factors; Female; Follow-Up Studies; Humans; Lung Neoplasms/prevention & control*; Male; Risk Factors; Cohort Studies; Smoking Cessation/methods*; Secondary Prevention*; Young Adult
  5. Roslani AC, Abdullah T, Arumugam K
    Asian Pac J Cancer Prev, 2012;13(1):237-41.
    PMID: 22502676
    OBJECTIVE: Screening for colorectal cancer using guaiac-based fecal occult blood tests (gFOBT) is well established in Western populations, but is hampered by poor patient compliance due to the imposed dietary restrictions. Fecal immunochemical tests (FIT) do not require dietary restriction, but are more expensive than gFOBT and therefore restrict its use in developing countries in Asia. However, Asian diets being low in meat content may not require diet restriction for gFOBT to achieve equivalent results. The objective of this study was to evaluate and compare the validity and suitability of gFOBT and FIT or a combination of the two in screening for colorectal neoplasias without prior dietary restriction in an Asian population.

    METHODS: Patients referred to the Endoscopic Unit for colonoscopy were recruited for the study. Stool samples were collected prior to bowel preparation, and tested for occult blood with both gFOBT and FIT. Dietary restriction was not imposed. To assess the validity of either tests or in combination to detect a neoplasm or cancer in the colon, their false positive rates, their sensitivity (true positive rate) and the specificity (true negative rate) were analyzed and compared.

    RESULTS: One hundred and three patients were analysed. The sensitivity for picking up any neoplasia was 53% for FIT, 40% for gFOBT and 23.3% for the combination. The sensitivities for picking up only carcinoma were 77.8% , 66.7% and 55.5%, respectively. The specificity for excluding any neoplasia was 91.7% for FIT, 74% for gFOBT and 94.5% for a combination, whereas for excluding only carcinomas they were 84%, 73.4% and 93.6%. Of the 69 with normal colonoscopic findings, FOBT was positive in 4.3%, 23.2 %and 2.9% for FIT, gFOBT, or combination of tests respectively.

    CONCLUSION: FIT is the recommended method if we are to dispense with dietary restriction in our patients because of its relatively low-false positivity and better sensitivity and specificity rates.

    MeSH terms: Adenocarcinoma/diagnosis*; Adenocarcinoma/epidemiology; Adenocarcinoma/prevention & control; Adenoma/diagnosis*; Adenoma/epidemiology; Adenoma/prevention & control; Asia/epidemiology; Colonoscopy; False Positive Reactions; Feces/chemistry*; Female; Guaiac; Humans; Immunoenzyme Techniques; Male; Mass Screening/methods*; Middle Aged; Occult Blood*; Patient Compliance; Predictive Value of Tests; Sensitivity and Specificity; Colorectal Neoplasms/diagnosis*; Colorectal Neoplasms/epidemiology; Colorectal Neoplasms/prevention & control; Caloric Restriction*
  6. Al-Naggar RA, Al-Naggar DH
    Asian Pac J Cancer Prev, 2012;13(1):243-6.
    PMID: 22502677
    OBJECTIVE: While the relatively common nature of female breast cancer has resulted in a high level of general awareness, male breast cancer is still comparatively unknown to the general public and to healthcare professionals. The objective of this study is to explore the perceptions and opinions about male breast cancer and male breast self-examination among male university students.

    METHODOLOGY: In-depth interviews were conducted among 36 male university students from the Management and Science University, Malaysia, selected by simple random sampling. The themes of the interview were: knowledge of male breast cancer and male breast self-examination, sources of knowledge and attitudes towards male BSE. The data obtained were classified into various categories and analyzed manually.

    RESULTS: The majority of participants mentioned that there is a low possibility for males to get breast cancer. They also believed that the cause of breast cancer among men is due to the carcinogens from cigarettes. The majority of participants mentioned that they know about breast self-examination from the mass media and that the presence of a lump in the breast is the main symptom of breast cancer in men. The majority of participants mentioned that they encourage their family members to practice breast self-examination but considered that BSE is not important for men because they have a low probability of getting breast cancer.

    CONCLUSIONS: Misconceptions regarding male breast cancer and breast self-examination among men still exist among male university students. Therefore special attention should be given to educate men about male breast cancer and male BSE.

    MeSH terms: Adult; Attitude to Health; Humans; Interviews as Topic; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Patient Compliance*; Perception; Prognosis; Surveys and Questionnaires; Students/psychology*; Universities; Breast Self-Examination*; Breast Neoplasms, Male/diagnosis*; Breast Neoplasms, Male/prevention & control; Breast Neoplasms, Male/psychology*; Qualitative Research; Early Detection of Cancer/psychology*; Young Adult
  7. Lim KH, Idzwan MF, Sumarni MG, Kee CC, Amal NM, Lim KK, et al.
    Asian Pac J Cancer Prev, 2012;13(1):343-6.
    PMID: 22502698
    Two methods of identifying smokers with high nicotine dependence, the heaviness of smoking index (HSI) and number of cigarettes per day (CPD) were compared with the Fagerstrom test for nicotine dependence (FTND). The HSI, CPD and the FTND were administered to 316 adult Malaysian male, daily smokers aged between 25-64 years old in the Malaysian NCD Surveillance-1 Survey using a two-stage stratified random sampling of enumeration blocks and living quarters, via an interview based on a validated questionnaire. The cut-off point for classification of high nicotine dependence on the HSI was a score of four or higher, and for the heavy smoking category, smoking more than 20 cigarettes per day. Classification using each method was compared with classification by the FTND (score of six or more) as the reference standard. Sensitivity, specificity and kappa statistics for concordance between both measures and the FTND were evaluated. The HSI gave a similar prevalence rate of high nicotine dependence as the FTND. There was substantial agreement between the HSI and the FTND (kappa=0.63.), with moderate sensitivity (69.8%) and high specificity (92.5%). However, prevalence of high nicotine dependence using the CPD was 7% lower than the FTND. The heavy smoking category also showed fair agreement with the FTND (kappa=0.45) and moderate sensitivity (67.0%), but specificity was high (86.9%). The findings indicate that the HSI can be used as an alternative to the FTND in screening for high nicotine dependence among daily smokers in large population-based studies, while CPD may not be a suitable alternative to the FTND.
    MeSH terms: Adult; Cross-Sectional Studies; Humans; Malaysia/epidemiology; Male; Middle Aged; Prognosis; Psychological Tests*; Psychometrics; Surveys and Questionnaires; Sensitivity and Specificity; Smoking/psychology*; Tobacco Use Disorder/diagnosis*; Tobacco Use Disorder/epidemiology*
  8. Chew KS, Kamarudin H, Hashim CW
    Int J Emerg Med, 2012 Apr 13;5:16.
    PMID: 22503137 DOI: 10.1186/1865-1380-5-16
    BACKGROUND: Conventionally, a nebulized short-acting β-2 agonist like salbutamol is often used as the reliever in acute exacerbations of asthma. However, recent worldwide respiratory outbreaks discourage routine use of nebulization. Previous studies have shown that combined budesonide/formoterol (Symbicort®, AstraZeneca) is effective as both a maintenance and reliever anti-asthmatic medication.

    METHODS: We performed a randomized, open-label study from March until August 2011 to compare the bronchodilatory effects of Symbicort® vs. nebulized salbutamol in acute exacerbation of mild to moderate asthmatic attack in an emergency department. Initial objective parameters measured include the oxygen saturation, peak expiratory flow rate (PEFR) and respiratory rate. During clinical reassessment, subjective parameters [i.e., Visual Analog Scale (VAS) and 5-point Likert scale of breathlessness] and the second reading of the objective parameters were measured. For the 5-point Likert scale, the patients were asked to describe their symptom relief as 1, much worse; 2, a little worse; 3, no change; 4, a little better; 5, much better.

    RESULTS: Out of the total of 32 patients enrolled, 17 patients (53%) were randomized to receive nebulized salbutamol and 15 (47%) to receive Symbicort®. For both treatment arms, by using paired t- and Wilcoxon signed rank tests, it was shown that there were statistically significant improvements in oxygen saturation, PEFR and respiratory rate within the individual treatment groups (pre- vs. post-treatment). Comparing the effects of Symbicort® vs. nebulized salbutamol, the average improvement of oxygen saturation was 1% in both treatment arms (p = 0.464), PEFR 78.67 l/min vs. 89.41 l/min, respectively (p = 0.507), and respiratory rate 2/min vs. 2/min (p = 0.890). For subjective evaluation, all patients reported improvement in the VAS (average 2.45 cm vs. 2.20 cm), respectively (p = 0.765). All patients in both treatment arms reported either "a little better" or "much better" on the 5-point Likert scale, with none reporting "no change" or getting worse.

    CONCLUSION: This study suggests that there is no statistical difference between using Symbicort® vs. nebulized salbutamol as the reliever for the first 15 min post-intervention.

  9. Oh WD, Lim PE, Seng CE, Sujari AN
    Bioresour Technol, 2012 Jun;114:179-87.
    PMID: 22503192 DOI: 10.1016/j.biortech.2012.03.065
    A kinetic model incorporating adsorption, desorption and biodegradation processes was developed to describe the bioregeneration of granular activated carbon (GAC) loaded with 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), respectively, in simultaneous adsorption and biodegradation processes. The model was numerically solved and the results showed that the kinetic model was well-fitted (R(2)>0.83) to the experimental data at different GAC dosages and at various initial 4-CP and 2,4-DCP concentrations. The rate of bioregeneration in simultaneous adsorption and biodegradation processes was influenced by the ratio of initial chlorophenol concentration to GAC dosage. Enhancement in the rate of bioregeneration was achieved by using the lowest ratio under either one of the following experimental conditions: (1) increasing initial chlorophenol concentration at constant GAC dosage and (2) increasing GAC dosage at constant initial chlorophenol concentration. It was found that the rate enhancement was more pronounced under the second experimental condition.
    MeSH terms: Adsorption; Bacteria, Aerobic/metabolism*; Biodegradation, Environmental; Charcoal/chemistry*; Chlorophenols/isolation & purification*; Chlorophenols/metabolism*; Computer Simulation; Kinetics; Models, Biological*; Models, Chemical*
  10. Hadi H, Makahleh A, Saad B
    PMID: 22503735 DOI: 10.1016/j.jchromb.2012.03.031
    A hollow fiber liquid phase microextraction (HF-LPME) in conjunction with reversed phase HPLC-UV method was developed for the extraction and determination of trace amounts of the antidiabetic drug, mitiglinide (MIT) in biological fluids. The drug was extracted from 10 mL aqueous sample (donor phase (DP)) into an organic phase impregnated in the pores of hollow fiber, followed by the back extraction into a second aqueous solution (acceptor phase (AP)) located in the lumen of the hollow fiber. Parameters influencing the extraction efficiency including the kind of organic solvent, composition of DP and AP, extraction time, stirring rate and salt addition were investigated and optimized. Under the optimized extraction conditions, high enrichment factors (210-fold), good linearity (5-1000 ng mL(-1)) and detection limit lower than 1.38 ng mL(-1) were achieved. Recoveries of spiked samples were in the range (88.3-96.3%) and (92.0-99.3%) for urine and plasma samples, respectively. The percent relative standard deviation (n=9) for the extraction and determination of three concentration levels (100, 400 and 800 ng mL(-1)) of MIT were less than 10.6% and 13.6% for urine and plasma samples, respectively. The developed method is simple, sensitive and has been successfully applied to the analysis of MIT in biological fluids.
    MeSH terms: Chromatography, High Pressure Liquid/methods*; Humans; Hydrogen-Ion Concentration; Sensitivity and Specificity; Sodium Chloride; Reproducibility of Results; Linear Models; Isoindoles/blood*; Isoindoles/urine*; Liquid Phase Microextraction/instrumentation; Liquid Phase Microextraction/methods*
  11. Tang SY, Sivakumar M, Ng AM, Shridharan P
    Int J Pharm, 2012 Jul 1;430(1-2):299-306.
    PMID: 22503988 DOI: 10.1016/j.ijpharm.2012.03.055
    The present study investigated the anti-inflammatory and analgesic activities of novel aspirin oil-in-water (O/W) nanoemulsion and water-in-oil-in-water (W/O/W) nano multiple emulsion formulations generated using ultrasound cavitation techniques. The anti-inflammatory activities of nanoemulsion and nano multiple emulsion were determined using the λ-carrageenan-induced paw edema model. The analgesic activities of both nanoformulations were determined using acetic acid-induced writhing response and hot plate assay. For comparison, the effect of pretreatment with blank nanoemulsion and reference aspirin suspension were also studied for their anti-inflammatory and antinociceptive activities. The results showed that oral administration of nanoemulsion and nano multiple emulsion containing aspirin (60 mg/kg) significantly reduced paw edema induced by λ-carrageenan injection. Both nanoformulations decreased the number of abdominal constriction in acetic acid-induced writhing model. Pretreatment with nanoformulations led to a significant increase in reaction time in hot plate assay. Nanoemulsion demonstrated an enhanced anti-inflammatory and analgesic effects compared to reference suspension while nano multiple emulsion exhibited a mild inhibitory effects in the three experimental animal model tests. The results obtained for nano multiple emulsion were relatively lower than reference. However, administration of blank nanoemulsion did not alter the nociceptive response significantly though it showed slight anti-inflammatory effect. These experimental studies suggest that nanoemulsion and nano multiple emulsion produced a pronounced anti-inflammatory and analgesic effects in rats and may be candidates as new nanocarriers for pharmacological NSAIDs in the treatment of inflammatory disorders and alleviating pains.
    MeSH terms: Administration, Oral; Analgesics/administration & dosage*; Analgesics/chemistry; Animals; Anti-Inflammatory Agents/administration & dosage*; Anti-Inflammatory Agents/chemistry; Aspirin/administration & dosage*; Aspirin/chemistry; Behavior, Animal/drug effects; Carrageenan; Chemistry, Pharmaceutical; Disease Models, Animal; Dose-Response Relationship, Drug; Drug Carriers*; Drug Compounding; Edema/chemically induced; Edema/prevention & control*; Emulsions; Male; Motor Activity/drug effects; Pain/chemically induced; Pain/physiopathology; Pain/prevention & control*; Pain/psychology; Polymers/chemistry*; Technology, Pharmaceutical/methods*; Time Factors; Ultrasonics*; Rats, Sprague-Dawley; Pain Threshold/drug effects; Acetic Acid; Nanotechnology; Rats; Nanoparticles*
  12. Lau GL, Sieo CC, Tan WS, Ho YW
    J Sci Food Agric, 2012 Oct;92(13):2657-63.
    PMID: 22505020 DOI: 10.1002/jsfa.5683
    Colibacillosis is one of the main causes of economic loss in the poultry industry worldwide. Although antibiotics have been used to control this infection, the emergence of antibiotic-resistant bacteria poses a threat to animal and human health. Phage therapy has been reported as one of the potential alternative methods to control bacterial infections. However, efficient phage therapy is highly dependent on the characteristics of the phage isolated. In the present study the characteristics of a lytic phage, ØEC1, which was found to be effective against the causative agent of colibacillosis in chickens in a previous in vivo study, are reported.
    MeSH terms: Animals; Chickens*; DNA, Viral; Escherichia coli/virology*; Escherichia coli Infections/microbiology; Escherichia coli Infections/prevention & control*; Escherichia coli Infections/veterinary; Hydrogen-Ion Concentration; Poultry Diseases/microbiology; Poultry Diseases/prevention & control*; Temperature; Microscopy, Electron, Transmission
  13. Ahmad H, Zulkifli MZ, Hassan NA, Harun SW
    Appl Opt, 2012 Apr 10;51(11):1811-5.
    PMID: 22505174 DOI: 10.1364/AO.51.001811
    We propose and demonstrate a tunable S-band multiwavelength Brillouin/Raman fiber laser (MBRFL) with a tuning range of between 1490 to 1530 nm. The proposed MBRFL is designed around a 7.7 km long dispersion compensating fiber in a simple ring configuration, acting as a nonlinear medium for the generation of multiple wavelengths from stimulated Brillouin scattering (SBS) and also as a nonlinear gain medium for stimulated Raman scattering (SRS) amplification. A laser source with a maximum power of 12 dBm acts as the Brillouin pump (BP), while two 1420 nm laser diodes with a total power of 26 dBm act as the Raman pumps (RPs). The MBRFL can generate a multiwavelength comb consisting of even and odd Stokes at an average power of -12 dBm and -14 dBm respectively, and by separating the even and odd Stokes outputs, a 20 GHz channel spacing is obtained between two consecutive wavelengths. Due to the four-wave mixing (FWM) effect, anti-Stokes lines are also observed. The multiwavelength comb generated is not dependent on the BP, thus providing high stability and repeatability and making it a highly potential source for many real-world applications. This is the first time, to the knowledge of the authors, that a tunable MBRFL has been developed using SRS to obtain gain in the S-band region.
    MeSH terms: Spectrum Analysis, Raman; Lasers, Semiconductor
  14. Soh KL, Shariff Ghazali S, Soh KG, Abdul Raman R, Sharif Abdullah SS, Ong SL
    J Infect Dev Ctries, 2012 Apr;6(4):333-9.
    PMID: 22505443
    INTRODUCTION: Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in intensive care units (ICUs). One factor causing VAP is aspiration of oral colonisation, which may result from poor oral care practice. Oral care using tooth brushing can prevent formulation of dental plaque that can be a reservoir for microbes causing VAP.
    METHODOLOGY: A cross-sectional survey was conducted among 124 nurses, using a self-administered questionnaire, to determine methods used, frequency, and attitude of nurses toward oral care provided to mechanically ventilated patients in Malaysian ICUs.
    RESULTS: Methods for oral care and their frequency of use varied between nurses even in the same unit. Cotton with forceps was used by 73.4% of the nurses. Some nurses used forceps and gauze (65%) or spatulas and gauze (36%). Toothbrushes were used by 50.8% of the nurses. Nurses in this hospital reported to have positive attitude toward providing oral care.
    CONCLUSIONS: The survey showed the need to have standardised oral care protocols in ICUs to improve quality of oral care provided to ventilated patients.
    MeSH terms: Attitude of Health Personnel; Cross-Sectional Studies; Humans; Intensive Care Units; Nurses; Oral Hygiene/methods*; Pilot Projects; Surveys and Questionnaires; Respiration, Artificial*; Pneumonia, Ventilator-Associated/prevention & control*
  15. Keng TC, Ng KP, Tan LP, Chong YB, Wong CM, Lim SK
    Ren Fail, 2012;34(6):804-6.
    PMID: 22506572 DOI: 10.3109/0886022X.2012.678208
    Peritonitis is well recognized as the Achilles tendon of peritoneal dialysis (PD). Reoccurrence of peritonitis due to the same organism, defined as either repeat or relapsing peritonitis under the 2005 guidelines by the International Society for Peritoneal Dialysis, often results in PD technique failure. Rothia dentocariosa, a low-virulent human oropharynx commensal, is a rarely reported pathogen in human infection, particularly infective endocarditis. R. dentocariosa PD-related peritonitis is exceedingly uncommon yet potentially results in repeat or relapsing peritonitis which requires catheter removal. We report a case of R. dentocariosa repeat and relapsing peritonitis in a PD patient who was treated successfully with antimicrobial therapy.
    MeSH terms: Actinomycetales Infections/drug therapy; Actinomycetales Infections/microbiology*; Humans; Male; Middle Aged; Peritoneal Dialysis/adverse effects*; Peritonitis/drug therapy; Peritonitis/microbiology*; Recurrence
  16. Lim L, Lee C, Chang E
    Int J Med Mushrooms, 2012;14(2):181-7.
    PMID: 22506578
    In general, Cordyceps sinensis is much more popular than C. militaris, though both species contain quite similar bioactive ingredients and exhibit medicinal activities. Many bioactive ingredients have been isolated from C. militaris, such as adenosine, cordycepin, D-mannitol, and exopolysaccharides. C. militaris is claimed to have extensive pharmacological properties, such as: anti-inflammatory; anti-fatigue; anti-bacterial; anti-diabetic; improve lung, liver, and kidney functions; to be beneficial for treating cancer as well as male and female sexual dysfunctions. C. militaris is fast gaining momentum for its so-called health benefits, and it is often used as a substitute for C. sinensis. In view of the growing popularity of C. militaris, nowadays C. militaris cultivation for stroma is also done. There is a great diversity of compounds from different strains of Cordyceps and different artificially cultivated products. This study is to determine the optimum culture parameters integrated with substrate of choice to bring the indoor-cultivated C. militaris to a higher and more consistent level of quality. To achieve the above objective, the resultant products after growth were analyzed for adenosine, cordycepin, and D-mannitol using the high-performance liquid chromatography method. The optimum culture condition to produce a high level of adenosine is by using millet as solid substrate. It must be cultivated in the dark for the first 7 days and harvested on day 40. The optimum culture condition to produce a high level of cordycepin is by using soybean as solid substrate. It must be cultivated in the dark for the first 14 days and harvested on day 50. While a high level of D-mannitol is achieved with millet as the solid substrate. It must be kept in the dark for the first 7 days and harvested on day 50. The adenosine level decreased and cordycepin increased from day 40 of culture to day 50 generally.
    MeSH terms: Adenosine/metabolism*; Adenosine/chemistry; Deoxyadenosines/metabolism*; Deoxyadenosines/chemistry; Mannitol/metabolism*; Mannitol/chemistry; Cordyceps/metabolism*; Fruiting Bodies, Fungal/metabolism*
  17. Singham GV, Vargo EL, Booth W, Othman AS, Lee CY
    Environ Entomol, 2012 Apr;41(2):426-31.
    PMID: 22507019 DOI: 10.1603/EN11228
    The fungus-growing termite, Macrotermes gilvus (Hagen), an indigenous species from Southeast Asia distributed from Myanmar to Indonesia and the Philippines, offers great potential as an ecological model system to elucidate the effects of geography on gene flow within this region. We used next generation sequencing (Roche 454 pyrosequencing) to identify microsatellite markers from the genomic DNA of M. gilvus. A modest sequencing volume generated 34,122 reads, with 1,212 (3.6%) reads contains microsatellites with di-, tri-, tetra-, penta-, and hexa-nucleotide repeat motifs. Thirty-seven loci were selected for primer development and tested for polymorphism across 22 colonies of M. gilvus. Eleven loci were found to be polymorphic with 2-4 alleles per locus. Observed and expected heterozygosities ranged between 0.091-0.727 and 0.090-0.540, respectively. Cross taxa amplification was successful across a panel of four related termite species and four multiplex groups were designed for future population genetic studies. These markers will open new avenues for the study of phylogeography and population genetics of this fungus-growing termite. This study also has effectively demonstrated the use of 454 pyrosequencing for the rapid development of informative microsatellite markers from a termite genome.
    MeSH terms: Animals; Genetic Markers; Geography; Polymorphism, Genetic*; Species Specificity; Sequence Analysis, DNA/methods; Microsatellite Repeats*; Isoptera/genetics*
  18. Ku PX, Abu Osman NA, Yusof A, Wan Abas WA
    J Biomech, 2012 Jun 1;45(9):1638-42.
    PMID: 22507349 DOI: 10.1016/j.jbiomech.2012.03.029
    Postural stability is crucial in maintaining body balance during quiet standing, locomotion, and any activities that require a high degree of balance performance, such as participating in sports and dancing. Research has shown that there is a relationship between stability and body mass. The aims of this study were to examine the impact that two variables had on static postural control: body mass index (BMI) and gender. Eighty healthy young adults (age=21.7±1.8 yr; height=1.65±0.09 m; mass=67.5±19.0 kg) participated in the study and the static postural control was assessed using the Biodex Balance System, with a 20 Hz sampling rate in the bipedic stance (BLS) and unipedic stance (ULS) for 30s. Five test evaluations were performed for each balance test. Postural control was found to be negatively correlated with increased adiposity, as the obese BMI group performed significantly poorer than the underweight, normal weight and overweight groups during BLS and ULS tests. The underweight, normal weight and overweight groups exhibited greater anterior-posterior stability in postural control during quiet stance. In addition, female displayed a trend of having a greater postural sway than male young adults, although it was evidenced in only some BMI groups. This study revealed that BMI do have an impact on postural control during both BLS and ULS. As such, BMI and gender-specific effects should be taken into consideration when selecting individuals for different types of sporting activities, especially those that require quiet standing.
    MeSH terms: Adult; Postural Balance/physiology*; Female; Humans; Male; Posture/physiology*; Sex Factors; Body Mass Index*; Young Adult
  19. Woo YL, Kyrgiou M, Bryant A, Everett T, Dickinson HO
    Gynecol Oncol, 2012 Aug;126(2):286-90.
    PMID: 22507534 DOI: 10.1016/j.ygyno.2012.04.012
    Gynaecological cancers are the second most common cancers among women. It has been suggested that centralised care improves outcomes but consensus is lacking. This systematic review assesses the effectiveness of centralisation of care for patients with gynaecological cancer, in particular, survival advantage.
    MeSH terms: Cancer Care Facilities/statistics & numerical data*; Centralized Hospital Services/statistics & numerical data*; Female; Genital Neoplasms, Female/therapy*; Gynecology/statistics & numerical data*; Humans; Medical Oncology/statistics & numerical data*; Survival Analysis
  20. Shyam S, Wai TN, Arshad F
    Asia Pac J Clin Nutr, 2012;21(2):201-8.
    PMID: 22507605
    This paper outlines the methodology to add glycaemic index (GI) and glycaemic load (GL) functionality to food DietPLUS, a Microsoft Excel-based Malaysian food composition database and diet intake calculator. Locally determined GI values and published international GI databases were used as the source of GI values. Previously published methodology for GI value assignment was modified to add GI and GL calculators to the database. Two popular local low GI foods were added to the DietPLUS database, bringing up the total number of foods in the database to 838 foods. Overall, in relation to the 539 major carbohydrate foods in the Malaysian Food Composition Database, 243 (45%) food items had local Malaysian values or were directly matched to International GI database and another 180 (33%) of the foods were linked to closely-related foods in the GI databases used. The mean ± SD dietary GI and GL of the dietary intake of 63 women with previous gestational diabetes mellitus, calculated using DietPLUS version3 were, 62 ± 6 and 142 ± 45, respectively. These values were comparable to those reported from other local studies. DietPLUS version3, a simple Microsoft Excel-based programme aids calculation of diet GI and GL for Malaysian diets based on food records.
    MeSH terms: Adult; Blood Glucose/analysis; Dietary Carbohydrates/adverse effects; Dietary Carbohydrates/analysis*; Dietary Carbohydrates/metabolism; Female; Food Analysis*; Humans; Hyperglycemia/blood; Hyperglycemia/ethnology; Hyperglycemia/metabolism; Hyperglycemia/prevention & control; Malaysia; Software; Diet Records; Databases, Factual*; Postprandial Period; Glycemic Index*
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