Browse publications by year: 2014

  1. Low YY, Gan CY, Kam TS
    J Nat Prod, 2014 Jun 27;77(6):1532-5.
    PMID: 24832351 DOI: 10.1021/np500289t
    Racemic andransinine (1), an indole alkaloid derivative obtained during isolation of alkaloids from Alstonia angustiloba and Kopsia pauciflora, was found to undergo spontaneous resolution when crystallized in EtOAc, forming racemic conglomerates (an equimolar mechanical mixture of enantiomerically pure individual crystals). X-ray analyses of the enantiomers (obtained from crystals from EtOAc solution and from chiral-phase HPLC) provided the absolute configuration of each enantiomer as (15R,16S,21R)-(+)-andransinine (1a or I+) and (15S,16R,21S)-(-)-andransinine (1b or I-).
    MeSH terms: Chromatography, High Pressure Liquid; Humans; Molecular Conformation; Stereoisomerism; Molecular Structure; Crystallography, X-Ray; Indole Alkaloids/isolation & purification*; Indole Alkaloids/chemistry; Alstonia/chemistry*
  2. Lim WM, Rajinikanth PS, Mallikarjun C, Kang YB
    Int J Nanomedicine, 2014;9:2117-26.
    PMID: 24833900 DOI: 10.2147/IJN.S57565
    The objectives of this study were to develop and characterize itraconazole (ITZ)-loaded nanostructured lipid carriers (NLCs) and to study their potential for drug delivery into the brain. Precirol(®) ATO 5 and Transcutol(®) HP were selected as the lipid phase, and Tween(®) 80 and Solutol(®) HS15 as surfactants. The ITZ-NLCs were prepared by a hot and high-pressure homogenization method. The entrapment efficiency for the best formulation batch was analyzed using high-performance liquid chromatography and was found to be 70.5%±0.6%. The average size, zeta potential, and polydispersity index for the ITZ-NLCs used for animal studies were found to be 313.7±15.3 nm, -18.7±0.30 mV, and 0.562±0.070, respectively. Transmission electron microscopy confirmed that ITZ-NLCs were spherical in shape, with a size of less than 200 nm. Differential scanning calorimetry and X-ray diffractometry analysis showed that ITZ was encapsulated in the lipid matrix and present in the amorphous form. The in vitro release study showed that ITZ-NLCs achieved a sustained release, with cumulative release of 80.6%±5.3% up to 24 hours. An in vivo study showed that ITZ-NLCs could increase the ITZ concentration in the brain by almost twofold. These results suggest that ITZ-NLCs can be exploited as nanocarriers to achieve sustained release and brain-targeted delivery.
    MeSH terms: Animals; Antifungal Agents/administration & dosage; Blood-Brain Barrier/metabolism*; Diglycerides/chemistry*; Drug Compounding/methods; Ethylene Glycols/chemistry*; Particle Size; Polyethylene Glycols/chemistry; Polysorbates/chemistry; Stearic Acids/chemistry; Tissue Distribution; Itraconazole/administration & dosage*; Itraconazole/pharmacokinetics*; Mice; Nanocapsules/administration & dosage; Nanocapsules/ultrastructure; Nanocapsules/chemistry*
  3. Huq AK, Jamal JA, Stanslas J
    PMID: 24834098 DOI: 10.1155/2014/782830
    Persicaria hydropiper (L.) Delarbre, belonging to Polygonaceae family, is a common weed found in most of the temperate countries including Bangladesh, China, Malaysia, and Japan. The plant is also referred to as "marsh pepper" or "smart weed." It appears to be a useful herb with evidence-based medicinal properties. The present work addresses the botanical description, traditional uses, phytochemistry, pharmacology, and toxicology of P. hydropiper. All plant parts have been commonly used in the traditional systems of medicines. Flavonoids are the major group of phytochemical components followed by drimane-type sesquiterpenes and sesquiterpenoids, as well as phenylpropanoids. Different extracts and plant parts showed remarkable pharmacological activities including antioxidant, antibacterial, antifungal, antihelminth, antifeedant, cytotoxicity, anti-inflammatory, antinociceptive, oestrogenicity, antifertility, antiadipogenicity, and neuroprotection. Mutagenicity and acute and subchronic toxicities of the plant were also reported. P. hydropiper has tremendous medicinal properties that could further be investigated for the development of evidence-based herbal products.
    MeSH terms: Neuroprotection; Analgesics; Anti-Bacterial Agents; Bangladesh; China; Flavonoids; Japan; Malaysia; Mutagens; Sesquiterpenes; Polygonum; Piper nigrum; Wetlands; Phytochemicals
  4. Eltyeb S, Salim N
    J Cheminform, 2014;6:17.
    PMID: 24834132 DOI: 10.1186/1758-2946-6-17
    The rapid increase in the flow rate of published digital information in all disciplines has resulted in a pressing need for techniques that can simplify the use of this information. The chemistry literature is very rich with information about chemical entities. Extracting molecules and their related properties and activities from the scientific literature to "text mine" these extracted data and determine contextual relationships helps research scientists, particularly those in drug development. One of the most important challenges in chemical text mining is the recognition of chemical entities mentioned in the texts. In this review, the authors briefly introduce the fundamental concepts of chemical literature mining, the textual contents of chemical documents, and the methods of naming chemicals in documents. We sketch out dictionary-based, rule-based and machine learning, as well as hybrid chemical named entity recognition approaches with their applied solutions. We end with an outlook on the pros and cons of these approaches and the types of chemical entities extracted.
  5. Madfa AA, Kadir MR, Kashani J, Saidin S, Sulaiman E, Marhazlinda J, et al.
    Med Eng Phys, 2014 Jul;36(7):962-7.
    PMID: 24834856 DOI: 10.1016/j.medengphy.2014.03.018
    Different dental post designs and materials affect the stability of restoration of a tooth. This study aimed to analyse and compare the stability of two shapes of dental posts (parallel-sided and tapered) made of five different materials (titanium, zirconia, carbon fibre and glass fibre) by investigating their stress transfer through the finite element (FE) method. Ten three-dimensional (3D) FE models of a maxillary central incisor restored with two different designs and five different materials were constructed. An oblique loading of 100 N was applied to each 3D model. Analyses along the centre of the post, the crown-cement/core and the post-cement/dentine interfaces were computed, and the means were calculated. One-way ANOVAs followed by post hoc tests were used to evaluate the effectiveness of the post materials and designs (p=0.05). For post designs, the tapered posts introduced significantly higher stress compared with the parallel-sided post (p<0.05), especially along the centre of the post. Of the materials, the highest level of stress was found for stainless steel, followed by zirconia, titanium, glass fibre and carbon fibre posts (p<0.05). The carbon and glass fibre posts reduced the stress distribution at the middle and apical part of the posts compared with the stainless steel, zirconia and titanium posts. The opposite results were observed at the crown-cement/core interface.
    MeSH terms: Computer Simulation; Dental Materials/chemistry*; Dental Stress Analysis/methods*; Humans; Incisor/physiology*; Incisor/surgery; Maxilla/physiology*; Maxilla/surgery; Models, Biological*; Post and Core Technique/instrumentation*; Pressure; Stress, Mechanical; Computer-Aided Design; Dental Prosthesis Design; Equipment Failure Analysis
  6. Lewis RS, Noor SM, Fraser FW, Sertori R, Liongue C, Ward AC
    J Immunol, 2014 Jun 15;192(12):5739-48.
    PMID: 24835394 DOI: 10.4049/jimmunol.1301376
    Cytokine-inducible SH2 domain-containing protein (CISH), a member of the suppressor of cytokine signaling family of negative feedback regulators, is induced by cytokines that activate STAT5 and can inhibit STAT5 signaling in vitro. However, demonstration of a definitive in vivo role for CISH during development has remained elusive. This study employed expression analysis and morpholino-mediated knockdown in zebrafish in concert with bioinformatics and biochemical approaches to investigate CISH function. Two zebrafish CISH paralogs were identified, cish.a and cish.b, with high overall conservation (43-46% identity) with their mammalian counterparts. The cish.a gene was maternally derived, with transcripts present throughout embryogenesis, and increasing at 4-5 d after fertilization, whereas cish.b expression commenced at 8 h after fertilization. Expression of cish.a was regulated by the JAK2/STAT5 pathway via conserved tetrameric STAT5 binding sites (TTCN3GAA) in its promoter. Injection of morpholinos targeting cish.a, but not cish.b or control morpholinos, resulted in enhanced embryonic erythropoiesis, myelopoiesis, and lymphopoiesis, including a 2- 3-fold increase in erythrocytic markers. This occurred concomitantly with increased activation of STAT5. This study indicates that CISH functions as a conserved in vivo target and regulator of STAT5 in the control of embryonic hematopoiesis.
    MeSH terms: Animals; Base Sequence; Embryo, Nonmammalian/immunology*; Hematopoiesis/genetics; Hematopoiesis/immunology*; Molecular Sequence Data; Zebrafish/genetics; Zebrafish/immunology*; Zebrafish Proteins/genetics; Zebrafish Proteins/immunology*; STAT5 Transcription Factor/genetics; STAT5 Transcription Factor/immunology*; Suppressor of Cytokine Signaling Proteins/genetics; Suppressor of Cytokine Signaling Proteins/immunology*; Janus Kinase 2/genetics; Janus Kinase 2/immunology
  7. Nordin AS, Kadir RA, Yahya NA, Zakaria H, Rashid RA, Habil MH
    Int Dent J, 2014 Aug;64(4):206-12.
    PMID: 24835463 DOI: 10.1111/idj.12110
    As a signatory to the World Health Organisation 2003 Framework Convention on Tobacco Control, Malaysia has policies in place and funded 300 public Quit clinics. Unfortunately, government dentists are not included to run tobacco dependence treatment. A cross-sectional exploratory survey was carried out to seek Malaysian dentists' opinion on their knowledge, perception and willingness to conduct tobacco dependence treatment. Participation was voluntary from those who attended a specially designed one-day, four-module workshop on tobacco cessation intervention. Data were collected using the Audience-Response-System equipment which tracked immediate responses covering four domains namely: smoking as a public health problem, smoking as an addiction, the role of dentists in the programme and confidence in conducting smoking cessation in the clinic. Sample comprised more female dentists (73.5%), mean age 33.6 (SD 8.99) years and with more than 3 years working experience. Findings indicated that the majority agreed Malaysia has a rising problem in the prevalence of smoking (71.6%) and predicted that it will affect mostly the young (81.9%). Only half of the dentists surveyed (58.9%) routinely recorded their patients' smoking habits. The majority (71.6%) believed that dentists are effective in helping their patient to stop smoking and 76.3% agreed that dentists should discuss the smoking habit with their patients; however, 60% agreed that doing so is too time consuming. In addition, only 24.7% knew of more ways to treat a smoking habit. The majority felt comfortable giving advice to patients about changing their habits (76.5%) or discussing treatment options (60.5%): 75% would opt for a combined programme of counselling and use of medication if they have to do, 15% would choose to go on counselling only, while 8% did not want to treat. In conclusion, the findings suggest that dentists have a strong potential to contribute significantly to providing smoking cessation treatment if adequately trained.
    MeSH terms: Adult; Attitude of Health Personnel*; Counseling; Cross-Sectional Studies; Dentist-Patient Relations; Education, Dental*; Female; Humans; Malaysia; Male; Middle Aged; Power (Psychology); Public Health; Self Concept; Smoking/prevention & control; Smoking Cessation; Practice Patterns, Dentists'; Tobacco Use Cessation*; Professional Role; Tobacco Use Cessation Products
  8. Gopinath SC, Tang TH, Chen Y, Citartan M, Lakshmipriya T
    Biosens Bioelectron, 2014 Oct 15;60:332-42.
    PMID: 24836016 DOI: 10.1016/j.bios.2014.04.014
    The ubiquitous nature of bacteria enables them to survive in a wide variety of environments. Hence, the rise of various pathogenic species that are harmful to human health raises the need for the development of accurate sensing systems. Sensing systems are necessary for diagnosis and epidemiological control of pathogenic organism, especially in the food-borne pathogen and sanitary water treatment facility' bacterial populations. Bacterial sensing for the purpose of diagnosis can function in three ways: bacterial morphological visualization, specific detection of bacterial component and whole cell detection. This paper provides an overview of the currently available bacterial detection systems that ranges from microscopic observation to state-of-the-art smartphone-based detection.
    MeSH terms: Bacteria/cytology*; Bacteria/isolation & purification*; Conductometry/instrumentation*; Equipment Design; Immunoassay/instrumentation*; Microscopy/instrumentation*; Sensitivity and Specificity; Reproducibility of Results; Biosensing Techniques/instrumentation*; Equipment Failure Analysis; Cell Phones*
  9. Ho YB, Zakaria MP, Latif PA, Saari N
    Sci Total Environ, 2014 Aug 1;488-489:261-7.
    PMID: 24836135 DOI: 10.1016/j.scitotenv.2014.04.109
    Repeated applications of animal manure as fertilizer are normal agricultural practices that may release veterinary antibiotics and hormones into the environment from treated animals. Broiler manure samples and their respective manure-amended agricultural soil samples were collected in selected locations in the states of Selangor, Negeri Sembilan and Melaka in Malaysia to identify and quantify veterinary antibiotic and hormone residues in the environment. The samples were analyzed using ultrasonic extraction followed by solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The broiler manure samples were found to be contaminated with at least six target analytes, namely, doxycycline, enrofloxacin, flumequine, norfloxacin, trimethoprim and tylosin. These analytes were detected in broiler manure samples with maximum concentrations reaching up to 78,516 μg kg(-1) dry weight (DW) (doxycycline). For manure-amended agricultural soil samples, doxycycline and enrofloxacin residues were detected in every soil sample. The maximum concentration of antibiotic detected in soil was 1331 μg kg(-1) DW (flumequine). The occurrence of antibiotics and hormones in animal manure at high concentration poses a risk of contaminating agricultural soil via fertilization with animal manure. Some physico-chemical parameters such as pH, total organic carbon (TOC) and metal content played a considerable role in the fate of the target veterinary antibiotics and progesterone in the environment. It was suggested that these parameters can affect the adsorption of pharmaceuticals to solid environmental matrices.
    MeSH terms: Agriculture; Animals; Anti-Bacterial Agents/analysis*; Chickens; Environmental Monitoring*; Malaysia; Manure/analysis*; Progesterone/analysis*; Soil/chemistry*; Soil Pollutants/analysis*; Veterinary Drugs/analysis*
  10. Yeong LT, Abdul Hamid R, Saiful Yazan L, Khaza'ai H, Awang Hamsin DE
    Nat Prod Res, 2014;28(22):2026-30.
    PMID: 24836304 DOI: 10.1080/14786419.2014.917415
    An isomeric mixture of α,β-amyrin (triterpene) and 2-methoxy-6-undecyl-1,4-benzoquinone (quinone) isolated from the Ardisia crispa root hexane (ACRH) extract was reported to possess anti-inflammatory properties in vivo. Considering the close association between inflammation and cancer, on top of the lack of antitumour study on those compounds, this study aimed to determine the potential of both compounds against tumour promotion in vitro, either as single agent or in combination. Triterpene and quinone compounds, as well as triterpene-quinone fraction (TQF) and ACRH were subjected to inhibition of Epstein-Barr virus-early antigen (EBV-EA) activation assay for that purpose. Compared with curcumin (positive control), inhibition against EBV-EA activation occurred in the order: ACRH>TQF ≥ curcumin>α,β-amyrin ≥ 2-methoxy-6-undecyl-1,4-benzoquinone. These findings reported, for the first time, the antitumor-promoting effect of α,β-amyrin and 2-methoxy-6-undecyl-1,4-benzoquinone from the roots of A. crispa, which was enhanced when both compounds act in synergy.
    MeSH terms: Antigens, Viral/drug effects*; Curcumin; Hexanes/chemistry*; Humans; Gas Chromatography-Mass Spectrometry; Oleanolic Acid/analogs & derivatives; Oleanolic Acid/isolation & purification; Oleanolic Acid/pharmacology; Oleanolic Acid/chemistry; Quinones/pharmacology*; Triterpenes/isolation & purification; Triterpenes/pharmacology*; Benzoquinones/isolation & purification; Benzoquinones/pharmacology; Benzoquinones/chemistry; Anticarcinogenic Agents/pharmacology*; Plant Roots/chemistry; Ardisia/chemistry*
  11. Dalaba MA, Akweongo P, Aborigo R, Awine T, Azongo DK, Asaana P, et al.
    Glob Health Action, 2014 Dec;7(1):23848.
    PMID: 28672456 DOI: 10.3402/gha.v7.23848
    Introduction The Government of Ghana introduced the National Health Insurance Scheme (NHIS) in 2003 to replace out-of-pocket (OOP) payment for health services with the inherent aim of reducing the direct cost of treating illness to households. Objective To assess the effects of the NHIS in reducing cost of treating malaria to households in the Kassena-Nankana districts of northern Ghana. Methods We conducted a cross-sectional survey between October 2009 and October 2011 in the Kassena-Nankana districts. A sample of 4,226 households was randomly drawn from the Navrongo Health and Demographic Surveillance System household database and administered a structured interview. The costs of malaria treatment were collected from the patient perspective. Results Of the 4,226 households visited, a total of 1,324 (31%) household members reported fever and 51% (675) reported treatment for malaria and provided information on where they sought care. Most respondents sought malaria treatment from formal health facilities 63% (424), with the remainder either self-medicating with drugs from chemical shops 32% (217) or with leftover drugs or herbs 5% (34). Most of those who sought care from formal health facilities were insured 79% (334). The average direct medical cost of treating malaria was GH¢3.2 (US$2.1) per case with the insured spending less (GH¢2.6/US$1.7) per case than the uninsured (GH¢3.2/US$2.1). The overall average cost (direct and indirect) incurred by households per malaria treatment was GH¢20.9 (US$13.9). Though the insured accounted for a larger proportion of admissions at health facilities 76% (31) than the uninsured 24% (10), the average amount households spent on the insured was less (GH¢4/US$2.7) than their uninsured counterparts (GH¢6.4/US$4.3). The difference was not statistically significant (p=0.2330). Conclusion Even though some insured individuals made OOP payments for direct medical care, there is evidence that the NHIS has a protective effect on cost (outpatient and in-patient) of malaria treatment.
  12. Hashim YZ, Worthington J, Allsopp P, Ternan NG, Brown EM, McCann MJ, et al.
    Food Funct, 2014 Jul 25;5(7):1513-9.
    PMID: 24836598 DOI: 10.1039/c4fo00090k
    The decreased cancer risk associated with consumption of olive oil may be due to the presence of phenolics which can modulate pathways including apoptosis and invasion that are relevant to carcinogenesis. We have previously shown that a virgin olive oil phenolics extract (OVP) inhibited invasion of HT115 colon cancer cells in vitro. In the current study we assessed the in vitro effects of OVP (25 μg mL(-1)) on HT115 cell migration, spreading and integrin expression. Furthermore, the anti-metastatic activity of OVP - at a dose equivalent to 25 mg per kg per day for 2, 8 or 10 weeks - was assessed in a Severe Combined ImmunoDeficiency (SCID) Balb-c mouse model. After 24 h OVP did not inhibit cell migration but significantly reduced cell spreading on fibronectin (65% of control; p < 0.05) and expression of a range of α and β integrins was modulated. In vivo, OVP by gavage significantly (p < 0.05) decreased not only tumour volume but also the number of metastases in SCID Balb-c mice. Collectively, the data suggest that - possibly through modulation of integrin expression - OVP decreases invasion in vitro and also inhibits metastasis in vivo.
    MeSH terms: Olive Oil; Animals; Antineoplastic Agents/pharmacology*; Colonic Neoplasms/drug therapy*; Disease Models, Animal; Female; Humans; Mice, Inbred BALB C; Neoplasm Metastasis; Phenols/pharmacology*; Plant Extracts/pharmacology*; Plant Oils/pharmacology*; Mice, SCID; Apoptosis/drug effects; Cell Line, Tumor; Mice
  13. Jiamsakul A, Kumarasamy N, Ditangco R, Li PC, Phanuphak P, Sirisanthana T, et al.
    J Int AIDS Soc, 2014;17:18911.
    PMID: 24836775 DOI: 10.7448/IAS.17.1.18911
    Adherence to antiretroviral therapy (ART) plays an important role in treatment outcomes. It is crucial to identify factors influencing adherence in order to optimize treatment responses. The aim of this study was to assess the rates of, and factors associated with, suboptimal adherence (SubAdh) in the first 24 months of ART in an Asian HIV cohort.
    MeSH terms: Adult; Asia/epidemiology; Female; Humans; Male; Middle Aged; Prospective Studies; Risk Factors; HIV Infections/drug therapy*; Anti-HIV Agents/therapeutic use*; Viral Load/statistics & numerical data; Medication Adherence/statistics & numerical data*; Self Report; Visual Analog Scale
  14. Nájera F, Hearn AJ, Ross J, Nathan S, Revuelta L
    Vet Res Commun, 2014 Sep;38(3):265-9.
    PMID: 24839119 DOI: 10.1007/s11259-014-9605-2
    Box-traps for capturing wild cats are widely used by researchers since it is one of the most effective methods for trapping these species. Although they are extensively utilised, the effects on the physiology of trapped felids remain unclear. Researchers frequently make judgements regarding the safety of such capture devices by examination of external injuries but often fail to take into consideration other physiological parameters. To assess the effects of capture events on selected serum biochemistry values of free-ranging Bornean leopard cats (Prionailurus bengalensis borneoensis) six free-ranging leopard cats (four males, two females) were trapped by using box-traps in Sabah, Malaysian Borneo. Blood was collected by jugular venipucture after chemical immobilization with a mixture of tiletamine and zolazepam. Blood was analysed for 17 serum biochemistry parameters. The most consistent and significantly higher value found in both sexes was aspartate aminotransferase (AST), followed by high mean value of alanine aminotransferase (ALT). Both mean values exceeded the upper limit of the reference range for captive leopard cats. These results demonstrate that captured leopard cats by box-traps undergo physical exertion and consequently some type of muscle injury/damage. Researchers and wildlife managers should be aware of the physiological response of trapped felids when using box-traps. Devices that facilitate the prompt removal of leopard cats from the traps would be useful for researchers to avoid further damage while live trapping this species.
    MeSH terms: Alanine Transaminase/blood; Animals; Animal Welfare*; Animals, Wild/blood; Animals, Wild/physiology*; Aspartate Aminotransferases/blood; Blood Chemical Analysis/veterinary; Borneo; Physical Exertion; Female; Male; Muscles/enzymology; Muscles/injuries; Restraint, Physical/adverse effects; Restraint, Physical/veterinary*; Felidae/blood; Felidae/physiology*
  15. Saha S, Chadha M, Al Mamun A, Rahman M, Sturm-Ramirez K, Chittaganpitch M, et al.
    Bull World Health Organ, 2014 May 01;92(5):318-30.
    PMID: 24839321 DOI: 10.2471/BLT.13.124412
    OBJECTIVE: To characterize influenza seasonality and identify the best time of the year for vaccination against influenza in tropical and subtropical countries of southern and south-eastern Asia that lie north of the equator.

    METHODS: Weekly influenza surveillance data for 2006 to 2011 were obtained from Bangladesh, Cambodia, India, Indonesia, the Lao People's Democratic Republic, Malaysia, the Philippines, Singapore, Thailand and Viet Nam. Weekly rates of influenza activity were based on the percentage of all nasopharyngeal samples collected during the year that tested positive for influenza virus or viral nucleic acid on any given week. Monthly positivity rates were then calculated to define annual peaks of influenza activity in each country and across countries.

    FINDINGS: Influenza activity peaked between June/July and October in seven countries, three of which showed a second peak in December to February. Countries closer to the equator had year-round circulation without discrete peaks. Viral types and subtypes varied from year to year but not across countries in a given year. The cumulative proportion of specimens that tested positive from June to November was > 60% in Bangladesh, Cambodia, India, the Lao People's Democratic Republic, the Philippines, Thailand and Viet Nam. Thus, these tropical and subtropical countries exhibited earlier influenza activity peaks than temperate climate countries north of the equator.

    CONCLUSION: Most southern and south-eastern Asian countries lying north of the equator should consider vaccinating against influenza from April to June; countries near the equator without a distinct peak in influenza activity can base vaccination timing on local factors.

    MeSH terms: Asia, Southeastern/epidemiology; Humans; Influenza, Human/epidemiology*; Influenza, Human/prevention & control; Influenza, Human/virology*; Influenza Vaccines; Nasal Mucosa/virology; Orthomyxoviridae/immunology; Orthomyxoviridae/isolation & purification*; Seasons; Tropical Climate
  16. Xu Y, Herrman H, Bentley R, Tsutsumi A, Fisher J
    Bull World Health Organ, 2014 May 01;92(5):348-55.
    PMID: 24839324 DOI: 10.2471/BLT.13.124677
    OBJECTIVE: To assess whether having a subsequent child had an effect on the mental health of Chinese mothers who lost a child during an earthquake.

    METHODS: A cross-sectional survey of bereaved mothers was conducted 30 to 34 months after the 2008 Sichuan earthquake using individual structured interviews to assess sociodemographic characteristics, post-disaster experiences and mental health. The interviews incorporated standardized psychometric measures of anxiety, depression, post-traumatic stress disorder (PTSD) and complicated grief (CG). Social support was also assessed. An adjusted model taking potential confounders into account was used to explore any association between psychological symptoms and the birth of a subsequent child.

    FINDINGS: The prevalence of psychological symptoms was higher in mothers who did not have a child after losing the first one. In an adjusted model, symptoms of anxiety (odds ratio, OR: 3.37; 95% confidence interval, CI: 1.51-7.50), depression (OR: 9.47; 95% CI: 2.58-34.80), PTSD (OR: 5.11; 95% CI: 2.31-11.34) and CG (OR: 10.73; 95% CI: 1.88-61.39) were significantly higher among the 116 women without a subsequent child than among the 110 mothers who had another child after bereavement. More than two thirds of the mothers with new infants had clinically important psychological symptoms.

    CONCLUSION: Women who have lost an only child in a natural disaster are especially vulnerable to long-term psychological problems, especially if they have reached an age when conception is difficult. Research should focus on developing and evaluating interventions designed to provide women with psychosocial support and reproductive services.

    MeSH terms: Adult; Mental Disorders/etiology*; Mental Disorders/epidemiology*; Bereavement*; China/epidemiology; Cross-Sectional Studies; Disasters; Female; Humans; Interviews as Topic; Mothers/psychology*; Parity; Social Support; Women's Health; Earthquakes
  17. Nafishah A, Asiah MN, Syimah AT, Mohd Zahari TH, Yasmin A, Normi M, et al.
    Indian J Hematol Blood Transfus, 2014 Jun;30(2):105-10.
    PMID: 24839364 DOI: 10.1007/s12288-012-0213-4
    The World Health Organization (WHO) recommend that all donated blood are to be screened for at least three viral infections [human immunodeficiency virus (HIV), hepatitis C virus (HCV), hepatitis B virus (HBV)]. The National Blood Centre, Kuala Lumpur (NBCKL) aims to reduce transfusion transmitted infections (TTI) as it still remains as one of the major risk for blood transfusion. A cross sectional study was conducted at the National Blood Centre, Kuala Lumpur from 1st January 2009 to 31st June 2010. Data from 581,020 donors were analyzed from year 2004 to 2008. All data were retrieved from NBCKL Blood Bank Information System (BBIS). A total of 201 repeat donors were included in the study based on the inclusion criteria but only 132 repeat donors agreed to participate. Information on sociodemographic, risk factors, knowledge of donors and high risk behavior were extracted from standardize questionnaire. Data were analyzed using SPSS version 14.0. The aim of this study was to determine the predictors of the seropositive infectivity among repeat blood donors at the NBCKL. The results showed Syphilis accounts for the highest and increasing seroconversion rate among other infections from 20.83 % in year 2004 to 44.6 % in year in year 2008. HIV and HCV infection also showed increasing seroconversion rate in 5 years' time from 6.41 % in year 2004 to 17.54 % in year 2008 and 4.8 % in year 2004 to 5.94 % in year 2008 respectively. However, HBV infection alone showed a decreasing seroconversion rate from 20.83 % in year 2004 to 10.4 % in year 2008. Level of donors' awareness regarding high risk factors (activities or behaviour) can lead to higher risk of TTI with significant p value in this predictors model(p 
    MeSH terms: Seroconversion; Blood Banks; Blood Donors; Blood Transfusion; Cross-Sectional Studies; Hepatitis B; Hepatitis B virus; Hepatitis C; Humans; Male; Risk Factors; Syphilis; World Health Organization; HIV Infections; Hepacivirus; Private Sector; Transfusion Reaction
  18. Wong JP, Wijaya S, Ting KN, Wiart C, Mustafa K, Shipton F, et al.
    PMID: 24839451 DOI: 10.1155/2014/492703
    If left untreated, hypercholesterolaemia can lead to atherosclerosis, given time. Plants from the Fabaceae family have shown the ability to significantly suppress atherosclerosis progression. We selected four extracts from Pithecellobium ellipticum, from the Fabaceae family, to be screened in a 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) assay. The ethanol extract, at a concentration of 500  μ g/mL, exhibited superior inhibition properties over the other extracts by demonstrating 80.9% inhibition, while 0.223  μ g/mL of pravastatin (control) showed 78.1% inhibition towards enzymatic activity. These findings led to the fractionation of the ethanol extract using ethyl acetate : methanol (95 : 5), gradually increasing polarity and produced seven fractions (1A to 7A). Fraction 7A at 150  μ g/mL emerged as being the most promising bioactive fraction with 78.7% inhibition. FRAP, beta carotene, and DPPH assays supported the findings from the ethanol extract as it exhibited good overall antioxidant activity. The antioxidant properties have been said to reduce free radicals that are able to oxidize lipoproteins which are the cause of atherosclerosis. Phytochemical screenings revealed the presence of terpenoid, steroid, flavonoid, and phenolic compounds as the responsible group of compound(s), working individually or synergistically, within the extract to prevent binding of HMG-CoA to HMG-CoA reductase.
  19. Rahman HA, Harun SW, Arof H, Irawati N, Musirin I, Ibrahim F, et al.
    J Biomed Opt, 2014 May;19(5):057009.
    PMID: 24839996 DOI: 10.1117/1.JBO.19.5.057009
    An enhanced dental cavity diameter measurement mechanism using an intensity-modulated fiber optic displacement sensor (FODS) scanning and imaging system, fuzzy logic as well as a single-layer perceptron (SLP) neural network, is presented. The SLP network was employed for the classification of the reflected signals, which were obtained from the surfaces of teeth samples and captured using FODS. Two features were used for the classification of the reflected signals with one of them being the output of a fuzzy logic. The test results showed that the combined fuzzy logic and SLP network methodology contributed to a 100% classification accuracy of the network. The high-classification accuracy significantly demonstrates the suitability of the proposed features and classification using SLP networks for classifying the reflected signals from teeth surfaces, enabling the sensor to accurately measure small diameters of tooth cavity of up to 0.6 mm. The method remains simple enough to allow its easy integration in existing dental restoration support systems.
    MeSH terms: Animals; Diagnostic Imaging/instrumentation; Diagnostic Imaging/methods; Dogs; Equipment Design; Fiber Optic Technology/instrumentation; Fiber Optic Technology/methods*; Models, Biological; Signal Processing, Computer-Assisted*; Tooth/chemistry*; Fuzzy Logic*
  20. Mohamed R, Jong PL, Nurul Irdayu I
    World J Microbiol Biotechnol, 2014 Sep;30(9):2427-36.
    PMID: 24840100 DOI: 10.1007/s11274-014-1668-2
    Aquilaria malaccensis produces agarwood in response to wounding and fungal attack. However, information is limited regarding Aquilaria's interaction with its diverse fungal community. In this study, time-related changes of three natural fungal colonizers in two wounded wild A. malaccensis were tracked, beginning a few hours after wounding up to 12 months. Using species-specific primers derived from their nrITS sequences in quantitative real-time PCR (qPCR), we quantified the amount of Cunninghamella bainieri, Fusarium solani and Lasiodiplodia theobromae. Because time is a major factor affecting agarwood quantity and quality, 14 wood samples were collected at different time points, i.e., 0-18 h, 2-13 days, 2-18 weeks, and 6-12 months after wounding. qPCR data revealed that the abundance of the three species decreased over time. The fungi were detected in high numbers during the first few hours and days after wounding (40- to 25,000-fold higher levels compared with initial counts) and in low numbers (<1- to 3,200-fold higher than initially) many months later. Consistent with its role in defense response, the accumulation of secondary metabolites at the wounding site could have caused the decline in fungal abundance. Succession patterns of the two trees were not identical, indicating that fungal populations may have been affected by tree environment and wound microclimate. Our results are important for understanding the diversity of microbial community in wild Aquilaria species and their association to wound-induced agarwood formation. Fungi could be secondary triggers to agarwood production in situations where trees are wounded in attempt to induce agarwood.
    MeSH terms: Ascomycota/growth & development*; Ascomycota/isolation & purification; Plant Diseases/microbiology*; Time Factors; Colony Count, Microbial*; Cunninghamella/growth & development*; Cunninghamella/isolation & purification; Thymelaeaceae/microbiology*; Biodiversity; Real-Time Polymerase Chain Reaction
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