Browse publications by year: 2019

  1. Krishnaveni Sritharan, Hong Koh Yiin, Karthik Krishnan, Khor Ben Nee, Mangair Karasi Manickam, Phua Cun Uei, et al.
    MyJurnal
    Malaysia is in the fifth place for world’s top ten retirement havens. In light to the combination of medical and the growth of old aged community in Malaysia, a few developers are experimenting with the concept of retirement village. The current study aimed to explore and gain a better understanding of the factors that will influence the intention of the middle aged adults in Johor to move into a retirement village. Based on the Theory of Planned Behaviour, the study found that Attitude and Subjective Norm has a positive and significant impact on the intention. Perceived Behavioural Control may be statistically significant but has not accurately predict the intention because of the absence of past behaviour or knowledge. The results indicated that those with tertiary education and who earns more that RM10,000 have intention to move in to the retirement village. The study itself have plausible explanation of the results and paved more opportunities for future research.
    MeSH terms: Adult; Aged; Attitude; Educational Status; Humans; Income; Malaysia; Middle Aged; Retirement; Knowledge; Behavior Control; Intention
  2. Abd Ganing Laengkang, Rohaidah Haron, Ab Razak Ab Karim
    MyJurnal
    Kajian ini memberi tumpuan terhadap salah satu kategori lakuan bahasa yang diperkenalkan oleh Searle (1969), iaitu lakuan ekspresi. Lakuan ekspresi ialah ujaran-ujaran yang menunjukkan pernyataan psikologi yang memberi gambaran tentang sikap dan perasaan penutur. Analisis kajian ini dilakukan terhadap ujaran-ujaran yang terdapat dalam 40 artikel kritikan Tun Dr. Mahathir dalam isu 1MDB. Melalui kajian ini, sebanyak lima kategori telah dikenal pasti, iaitu lakuan pernyataan, lakuan kritikan, lakuan rujukan, lakuan arahan, dan lakuan ekspresi. Namun demikian, kajian ini hanya memberi tumpuan terhadap lakuan bahasa ekspresi. Analisis mendapati lakuan ekspresi menunjukkan bilangan ujaran paling sedikit berbanding dengan kategori lakuan bahasa yang lain, iaitu sebanyak 20 kekerapan. Lakuan pernyataan mencatatkan kekerapan tertinggi, dengan jumlah kekerapan sebanyak 1302 ujaran, diikuti oleh lakuan kritikan di tempat kedua dengan kekerapan sebanyak 828. Lakuan rujukan dan lakuan arahan masing-masing dengan kekerapan sebanyak 297 dan 128. Hal ini menunjukkan bahawa Tun Dr. Mahathir melakukan kritikan tanpa mengutamakan ekspresi perasaan atau emosi. Sebanyak tujuh sublakuan ekspresi yang ditemui, iaitu lakuan bimbang, hairan, ucapan selamat, kehendak, enggan, minta maaf, dan ucapan terima kasih. Hal ini merumuskan bahawa dalam kritikan, khususnya kritikan bertulis, Tun Dr. Mahathir tidak menunjukkan ekspresi emosi yang berlebihan.
    MeSH terms: Animals; Color; Pallor; Bivalvia
  3. Nurzaimah Asalal, Haris Abd Wahab
    MyJurnal
    This study aimed to investigate the challenges faced by students enrolled in social work program in public universities in regard to fulfillment of education values and social work ethics. The study applied quantitative methods which involved 279 final year students who participated in social work program in Malaysian Public Universities. The findings showed that students who enrolled in this program did not face any challenges to fulfill the values education standards and social work ethics. However, the Chi Square result revealed that there is significant correlation (P ≤ 0.05) between the challenges faced by students and their ability to master the value and social work ethics. The study aspired to produce students who are able to master the values and work ethics standards during their study years. Students’ good command in value and social work ethics would help them to be prepared to face the real situations.
    MeSH terms: Educational Status; Humans; Personal Satisfaction; Reference Standards; Social Work; Students; Universities
  4. Mohamad Izzuan Mohd ishar, Moh Khata Jabor
    MyJurnal
    Kemajuan negara dan perkembangan bangsa adalah berdasarkan sistem pendidikan yang diamalkan. Pendidikan yang baik dapat melahirkan generasi yang bertanggungjawab dan menghargai kehidupan. Hal ini telah mencetuskan Wawasan Pendidikan Negara yang merupakan inti pati kepada Wawasan 2020. Usaha menyemai nilai murni dan perubahan pendidikan dalam kalangan pelajar kini turut terletak di bahu guru. Sifat kepimpinan yang terdapat pada guru amat penting dalam pembentukan wawasan pendidikan terutama gaya kepimpinan yang digunakan terhadap pelajar. Berdasarkan hala tuju yang telah digariskan, guru perlu menggunakan gaya kepimpinan yang diamalkan secara menyeluruh bagi memastikan segala aktiviti atau strategi yang dirancang mengikut hala tuju tersebut. Peranan seorang guru dalam membentuk wawasan ini juga membantu memberi kefahaman kepada guru dalam segala tindak tanduk yang perlu dilakukannya bagi mencapai Wawasan Pendidikan Negara
    MeSH terms: Animals; Spiders; Malvaceae; Orchidaceae
  5. Hussein Z, Bidin ML, Alias A, Thiagarajan M, Latif KA, Ratnasingam J, et al.
    J ASEAN Fed Endocr Soc, 2019;34(1):8-14.
    PMID: 33442131 DOI: 10.15605/jafes.034.01.03
    In Malaysia, acromegaly is under-recognised with only 10-15% of the expected number of cases from prevalence estimates, having been diagnosed and managed in established endocrine centres with access to multidisciplinary care. This is mainly due to lack of awareness and standardised approach in diagnosing this disease resulting in delay in diagnosis and management with suboptimal treatment outcomes. This first Malaysian consensus statement on the diagnosis and management of acromegaly addresses these issues and is based on current best practices and latest available evidence so as to reduce the disease burden on acromegaly patients managed in the Malaysian healthcare system.
    MeSH terms: Acromegaly; Delivery of Health Care; Humans; Malaysia; Reference Standards; Causality; Prevalence; Treatment Outcome; Cost of Illness; Consensus; Interdisciplinary Studies
  6. Lee YL, Wu LL
    J ASEAN Fed Endocr Soc, 2019;34(1):22-28.
    PMID: 33442133 DOI: 10.15605/jafes.034.01.05
    Objectives: Diagnosis of Turner syndrome in Malaysia is often late. This may be due to a lack of awareness of the wide clinical variability in this condition. In our study, we aim to examine the clinical features of all our Turner patients during the study period and at presentation.

    Methodology: This was a cross-sectional study. Thirty-four (34) Turner patients were examined for Turner-specific clinical features. The karyotype, clinical features at presentation, age at diagnosis and physiologic features were retrieved from their medical records.

    Results: Patients with 45,X presented at a median age of 1 month old with predominantly lymphoedema and webbed neck. Patients with chromosome mosaicism or structural X abnormalities presented at a median age of 11 years old with a broader clinical spectrum, short stature being the most common presenting clinical feature. Cubitus valgus deformity, nail dysplasia and short 4th/5th metacarpals or metatarsals were common clinical features occurring in 85.3%-94.1% of all Turner patients. Almost all patients aged ≥2 years were short irrespective of karyotype.

    Conclusion: Although short stature is a universal finding in Turner patients, it is usually unrecognised till late. Unlike the 45,X karyotype, non-classic Turner syndrome has clinical features which may be subtle and difficult to discern. Our findings underscore the importance of proper serial anthropometric measurements in children. Awareness for the wide spectrum of presenting features and careful examination for Turner specific clinical features is crucial in all short girls to prevent a delay in diagnosis.

    MeSH terms: Aged; Child; Child, Preschool; Cross-Sectional Studies; Dwarfism; Female; Humans; Infant; Karyotyping; Lymphedema; Malaysia; Medical Records; Metatarsal Bones; Metatarsus; Mosaicism; Turner Syndrome; Metacarpal Bones; Karyotype
  7. Ng D, Noor NM, Yong SL
    J ASEAN Fed Endocr Soc, 2019;34(1):29-35.
    PMID: 33442134 DOI: 10.15605/jafes.034.01.06
    Objectives: To determine the prevalence of hypoglycaemia using continuous glucose monitoring system (CGMS) among insulin-treated pregnant women with diabetes whose glycosylated haemoglobin (HbA1c) were <6.0% and identify the risk factors associated with hypoglycaemia occurrence.

    Methodology: We conducted a cross-sectional study using 6-days CGMS to detect the prevalence of hypoglycaemia in 31 insulin-treated pregnant women with diabetes who achieved HbA1c <6.0%. Patients were required to log-keep their self-monitoring blood glucose (SMBG) readings and hypoglycaemia events.

    Results: Eight women experienced confirmed hypoglycaemia with additional seven experienced relative hypoglycaemia, giving rise to prevalence rate of 45.2% (one had both confirmed and relative hypoglycaemia). Nine relative hypoglycaemia and 17 confirmed hypoglycaemic events were recorded. Sixteen (94%) out of 17 confirmed hypoglycaemia events recorded by CGMS were asymptomatic and were missed despite performing regular SMBG. Nocturnal hypoglycaemia events were recorded in seven women. Univariable analysis did not identify any association between conventional risk factors and hypoglycaemia events in our cohort.

    Conclusion: Insulin-treated pregnant women with diabetes who achieved HbA1c <6.0% were associated with high prevalence of hypoglycaemia. Asymptomatic hypoglycaemia is common in our cohort and frequently missed despite regular SMBG. Present study did not identify any association between conventional risk factors and hypoglycaemia events in our cohort.

    MeSH terms: Blood Glucose; Cross-Sectional Studies; Diabetes Mellitus, Type 1; Female; Humans; Hypoglycemia; Hypoglycemic Agents; Pregnancy; Risk Factors; Blood Glucose Self-Monitoring; Cohort Studies; Prevalence; Pregnant Women; Insulins
  8. Lim KG, Nantha YS, Kasim NH, Ramamoothy T, Yam A, Lim EW, et al.
    J ASEAN Fed Endocr Soc, 2019;34(1):56-61.
    PMID: 33442137 DOI: 10.15605/jafes.034.01.09
    Background: Medical students at the International Medical University (IMU), Seremban, Malaysia were required to assess patients at home over a period of two years as a part of their curriculum. The students conducted six visits to educate their patients and help them utilize available resources to manage their disease.This study aims to examine whether patients with diabetes visited improve their control of their disease, specifically in terms of their HbA1c measurement.

    Methodology: We used a retrospective, matched before and after study design to prevent biased levels of effort by students conducting the home visits over two years. Information was obtained through reports written by IMU students. Convenient sampling was used to select outpatients undergoing treatment 'as usual' from a health clinic and were subsequently matched as controls.

    Results: There was a significant decrease in the mean HbA1c among 57 patients with diabetes who were CFCS subjects [from 8.4% (68 mmol/mol) to 7.3% (57 mmol/mol) p<0.001], while the mean HbA1c levels among 107 matched control subjects rose significantly from 7.9% (63 mmol/mol) to 8.3% (67 mmol/mol) (p=0.019) over a similar period. The two groups were controlled for most biological and socioeconomic variables except for comorbidities, diabetic complications and medication dose changes between groups.

    Conclusion: Behavioural intervention in the form of home visits conducted by medical students is an effective tool with a dual purpose, first as a student educational initiative, and second as a strategy to improve outcomes for patients with diabetes.

    MeSH terms: Curriculum; Diabetes Mellitus; House Calls; Humans; Malaysia; Outpatients; Research Design; Retrospective Studies; Socioeconomic Factors; Students, Medical; Universities; Comorbidity; Diabetes Complications
  9. Krishnan GD, Yahaya N, Yahya M
    J ASEAN Fed Endocr Soc, 2019;34(1):92-94.
    PMID: 33442142 DOI: 10.15605/jafes.034.01.14
    A 31-year-old male, apparently well, presented with typical chest pain. His ECG showed ST-elevation from V1-V4 and echocardiogram revealed anteroseptal wall hypokinesia with ejection fraction of 45%. Normal coronary arteries were seen on coronary angiogram. A thyroid function test showed elevated free T4 levels with suppressed thyroid stimulating hormone (TSH). Treatment with thionamides and beta-blockers improved symptoms. Upon review 4 months later he was well. Repeat echocardiogram showed good ejection fraction with no hypokinetic area.
    MeSH terms: Arrhythmias, Cardiac; Chest Pain; Coronary Vessels; Echocardiography; Electrocardiography; Male; Stroke Volume; Thyroid Function Tests; Thyrotropin; Coronary Angiography; Hypokinesia; ST Elevation Myocardial Infarction
  10. Kang W, Singarayar C, Wahab NA, Sukor N, Kamaruddin NA
    J ASEAN Fed Endocr Soc, 2019;34(1):95-98.
    PMID: 33442143 DOI: 10.15605/jafes.034.01.15
    An adrenal mass can be a diagnostic challenge as it is not easy to differentiate the adrenal glands from other adrenal pseudotumours with only radio-imaging. We report a 28-year-old patient who was diagnosed radiologically as an adrenal cortical carcinoma after he presented with abdominal pain and fullness. Biochemically, he demonstrated secondary hyperaldosteronism. Intra-operatively there was a huge mass, inferior to a normal right adrenal, which was histopathologically proven to be a dedifferentiated liposarcoma.
    MeSH terms: Adrenal Cortex Neoplasms; Adrenal Glands; Adult; Diagnostic Imaging; Humans; Hyperaldosteronism; Liposarcoma; Male; Abdominal Pain; Adrenocortical Carcinoma
  11. Araneta MR
    J ASEAN Fed Endocr Soc, 2019;34(2):126-133.
    PMID: 33442147 DOI: 10.15605/jafes.034.02.02
    Type 2 diabetes prevalence is rising rapidly in Southeast Asia (SEA) where urbanization and adoption of 'western' behavioral lifestyles are attributed as predominant risk factors. The Southeast Asian diaspora to the United States has resulted in a sizable portion of migrant and US born SEAs, with approximately 4 million Filipino Americans, 2 million Vietnamese-Americans, Cambodians (330,000), and Thai (300,000) as the most populous. Their longer exposure to a western lifestyle and participation in clinical studies with other racial/ethnic groups, provide opportunities to evaluate etiologic factors which might inform trends and intervention opportunities among residents of Southeast Asia. Epidemiologic studies in the US have identified higher T2D prevalence among Filipinos (16.1%) compared to groups perceived to be at highest risk for T2D, namely Latinos (14.0%), Black (13.7%), and Native Americans (13.4%), while SEAs (including Burmese, Cambodian, Indonesian, Laotian, Malaysian, and Thai, 10.5%) and Vietnamese (9.9%) had higher T2D risk compared to Whites (7.7%), despite their absence of general obesity. Asian-Americans, including SEAs, East and South Asians, collectively have higher rates of undiagnosed T2D compared to other racial/ethnic groups in the US. Almost half (44%) of Filipinos with newly diagnosed T2D have isolated post-challenge hyperglycemia and will remain undiagnosed if current screening practices remain limited to measures of glycosylated hemoglobin and fasting plasma glucose. The University of California San Diego Filipino Health Study found excess visceral adipose tissue accumulation, low ratio of muscle to total abdominal mass area, low adiponectin concentration, multiparity (≥ 6 live births), and sleep insufficiency (<7 hours) to be unique T2D risk factors among Filipino-American women, even after adjusting for established T2D risk factors including hypertension and parental history of T2D. Social determinants such as low educational attainment (less than college completion), and sustained social disadvantage during childhood and adulthood were independently associated with T2D risk. Gestational diabetes is a known risk factor for future T2DM among women; Northern California data shows that following Asian Indians, gestational diabetes was highest among Filipina and SEA parturients, who had twice the GDM prevalence as Black, Hispanic, and White women. Identification of novel T2D risk factors among SEAs may guide early diagnosis, inform pathophysiology, and identify unique opportunities for T2D prevention and management.
    MeSH terms: Adult; Asian Americans; African Americans; Blood Glucose; Child; Diabetes Mellitus, Type 2; Fasting; Female; Hispanic Americans; Humans; Hyperglycemia; Hypertension; Indonesia; Life Style; Muscles; Obesity; Parents; Parity; Pregnancy; Risk Factors; Sleep Deprivation; Thailand; Transients and Migrants; United States; Urbanization; Prevalence; Diabetes, Gestational; Early Diagnosis; Intra-Abdominal Fat; Live Birth; Adiponectin; Human Migration; Social Determinants of Health
  12. Cheah JM, Ng D, Low MY, Foo SH
    J ASEAN Fed Endocr Soc, 2019;34(2):206-209.
    PMID: 33442157 DOI: 10.15605/jafes.034.02.12
    Thyroid crisis is a life-threatening form of thyrotoxicosis characterized by multi-system dysfunction. Therapeutic plasma exchange has been reported to be effective in removing excessive circulating thyroid hormones. We present a 46-year-old female with recently diagnosed Graves' disease associated with thyrotoxic cardiomyopathy admitted for thyroid crisis complicated by propranolol-induced circulatory collapse, acute kidney injury and ischemic hepatitis. The tachyarrhythmia was refractory to conventional therapy. Initiation of TPE resulted in rapid clinical and biochemical stabilization.
    MeSH terms: Female; Graves Disease; Hepatitis; Cardiomyopathies; Plasma Exchange; Plasmapheresis; Propranolol; Shock; Tachycardia; Thyroid Crisis; Thyroid Diseases; Thyroid Hormones; Thyrotoxicosis; Acute Kidney Injury
  13. Shahar S, Lim KP, Mohamad M
    J ASEAN Fed Endocr Soc, 2019;34(2):229-232.
    PMID: 33442162 DOI: 10.15605/jafes.034.02.17
    Eight cases of parathyroid carcinoma were identified (8 females; median age 45 years, range 28-72). Half of whom were diagnosed preoperatively. Hypercalcemic symptoms were seen in 87.5% of the patients and the main complication was nephrolithiasis. At presentation, the median calcium was 3.675 mmol/L, median phosphate of 0.68 mmol/L, median intact parathyroid hormone (iPTH) was 211 pmol/L. Five patients had regional nodes metastasis and 1 had distant metastasis to the lungs. Parathyroid gland invasion to adjacent structures was seen in 62.5% of cases while another 62.5% showed capsular or vascular infiltration on histology with median tumour size of 3.2 cm. Recurrent hypercalcemia occurred in 50% of the patients with median time of recurrence of 21 months. In this case series, we found that patients with severe hypercalcemia and high iPTH also exhibited a high index suspicion of PC.
    MeSH terms: Adult; Aged; Calcium; Calcium, Dietary; Female; Humans; Hypercalcemia; Kidney Calculi; Lung; Middle Aged; Neoplasm Recurrence, Local; Parathyroid Glands; Parathyroid Hormone; Parathyroid Neoplasms; Phosphates
  14. Masoomi MA, Al-Shammeri I, Kalafallah K, Elrahman HMA, Ragab O, Ahmed E, et al.
    Medicine (Baltimore), 2019 Jan;98(4):e14207.
    PMID: 30681596 DOI: 10.1097/MD.0000000000014207
    Many discrepancy in selection of proper filter and its parameters for individual cases exists. The authors investigate the impact of the most common filters on patient NM images with coronary artery disease (CAD), and compare the results with the computerized tomography (CT)-Angio and angiography for accuracy.The investigation initiated by performing various single photon emission computerized tomography (SPECT)/CT scan of the national electrical manufacturers association chest phantoms having hot and cold inserts. Data acquired on GE 670 PRO SPECT/CT; 360Ø, 64 frames, 60 seconds, low energy high resolution (LEHR) 128, low energy general purpose (LEGP) with CT attenuation (120 kV and 170 mA). The images reconstructed with filtered back projection and ITERATIVE ordered-subset expectation maximization utilizing filters; Hann, Butterworth, Metz, Hamming, and Wiener. The Image contrast was calculated to assess absolute nearness of the inserts. Based on the preliminary results, then scans of 92 patients with CAD; 64 males and 28 females, age 41 to 77 years old, who had been reported earlier reprocessed with the nominated filter and were reported by 2 NM expert. The results compared to the earlier reports and to the CT-Angio and angiography.The optimization suggested 3 filters; Wiener (Wi), Metz and Butterworth (But) provide the highest contrast (99- 66.4%) and (81- 32%) for the cold and hot inserts respectively, with the (Wi) filter to be the better option. The reprocessed patients scan with the (Wi) presented an elevated diagnostic accuracy, correlated well with the CT-Angio and angiography results (P 
    MeSH terms: Adult; Aged; Female; Humans; Male; Middle Aged; Radiographic Image Enhancement/instrumentation*; Tomography, Emission-Computed, Single-Photon/instrumentation*; Tomography, Emission-Computed, Single-Photon/methods; Phantoms, Imaging/statistics & numerical data; Single Photon Emission Computed Tomography Computed Tomography/methods; Single Photon Emission Computed Tomography Computed Tomography/statistics & numerical data; Computed Tomography Angiography*
  15. Mat Zauki NA, Satyanarayana B, Fairuz-Fozi N, Nelson BR, Martin MB, Akbar-John B, et al.
    J Environ Manage, 2019 Feb 15;232:1012-1020.
    PMID: 33395753 DOI: 10.1016/j.jenvman.2018.12.002
    Carcinoscorpius rotundicauda and Tachypleus gigas may co-exist and share common spawning grounds elsewhere but at Balok (East Coast of Peninsular Malaysia), C. rotundicauda is an understudied species. Neglected as research candidate because of inaccessible spawning grounds, smaller size and less commercial value than T. gigas and also, difficulty to attain from the wild has made C. rotundicauda population status remaining unidentified at Balok. This standpoint drove the present attempt because anthropic activities like structure placement and mining are point-source for runoffs that load sediments into Balok River. While erosion-accretion events have altered Balok River width, the shore sediments in Balok Beach were transitioned between medium-fine and fine sand between years 2012 and 2016. Eventually by year 2016, the C. rotundicauda were depositing 5117 eggs in 91 nests from 200 to 1000 m range along this corridor facing South China Sea. From this yield, C. rotundicauda released 2880 eggs in 56 nests during the Southwest monsoon, 1254 eggs in 19 nests during the Northeast monsoon and 983 eggs in 16 nests during the Inter-monsoon seasons. Though female C. rotundicauda opted to lay their eggs in shallow burrows at lower shorelines, the absence of erosion and substantial silt and clay (>20%) deposition facilitates C. rotundicauda embryogenesis with brief periods of temperature and salinity shocks during day-time falling tides. This encourages C. rotundicauda to emerge with increasing abundance and carry out bi-monthly spawning at Balok Beach. In short, shore restoration initiatives like systematic boat docking, proper disposal of nets and waste and, periodic fish-catching operations were effectively led by the Balok fisher citizen scientist. This successful community joint-cooperation proves that citizen-led caretaking of degraded beaches offers marine life protection and are practical for coastal area management especially at areas where other oviparous animals such as turtles and crocodiles are harboured.
    MeSH terms: Accidental Falls; Alligators and Crocodiles; Animals; China; Eggs; Female; Horseshoe Crabs; Malaysia; Seasons; Ships; Temperature; Turtles; Rivers; Embryonic Development; Oviparity; Salinity
  16. Lee SY, Ng WL, Hishamuddin MS, Mohamed R
    Mitochondrial DNA B Resour, 2019;4(1):19-20.
    PMID: 33365402 DOI: 10.1080/23802359.2018.1535848
    Known for its durable timber quality, Neobalanocarpus heimii (King) Ashton is a highly sought after tree species endemic to the Malay Peninsula. Due to its scarcity and high value, the tree is classified under the IUCN Red List categories of Vulnerable. In this study, we assembled the complete chloroplast (cp) genome of N. heimii using data from high-throughput Illumina sequencing. The Chengal cp genome is 151,191 bp in size and includes two inverted repeat regions of 23,721 bp each, which is separated by a large single copy region of 83,801 bp and a small single copy region of 19,948 bp. A total of 130 genes were predicted, including 37 tRNA, 8 rRNA, and 85 protein-coding genes. Phylogenetic analysis placed N. heimii within the order Malvales.
    MeSH terms: Chloroplasts; Malaysia; Phylogeny; RNA, Ribosomal; RNA, Transfer; Genome, Chloroplast; High-Throughput Nucleotide Sequencing
  17. Rosli N, Sitam FT, Rovie-Ryan JJ, Gan HM, Lee YP, Hartini Ithnin, et al.
    Mitochondrial DNA B Resour, 2019 Jul 13;4(2):2535-2536.
    PMID: 33365614 DOI: 10.1080/23802359.2019.1640085
    Here, we present the first complete mitochondrial genome of Malayan Gaur (Bos gaurus hubbacki) inferred using next-generation sequencing. The mitogenome is 16,367 bp in length with the structural organization of a typical bovine mitochondrial arrangement comprising 13 protein-coding genes, 21 tRNAs, and 2 rRNAs. No internal stop codon was found in the protein-coding genes. Phylogenetic tree analysis revealed that Malayan gaur is more closely related to Burmese banteng instead of gaur.
    MeSH terms: Animals; Cattle; Mitochondria; Phylogeny; RNA, Ribosomal; RNA, Transfer; Codon, Terminator; Genome, Mitochondrial; High-Throughput Nucleotide Sequencing
  18. Tan MP, Amornsakun T, Siti Azizah MN, Habib A, Sung YY, Danish-Daniel M
    Mitochondrial DNA B Resour, 2019 Sep 12;4(2):2966-2969.
    PMID: 33365813 DOI: 10.1080/23802359.2019.1662741
    Eighty-four specimens collected from 13 populations from Malaysia, Thailand, and Vietnam were analysed, revealing 21 putative haplotypes with overall estimated haplotype and nucleotide diversities of 0.79 and 0.0079, respectively. High levels of diversity and an absence of founder effects were observed among populations in peninsular Malaysia. In contrast, populations from Sarawak exhibited low genetic diversity, which is a typical sign of colonies introduced from a single source. Historical translocation of Trichopodus pectoralis from Thailand to Malaysia, as well as to the Philippines, Indonesia, and Myanmar was apparent. Historical introduction of T. pectoralis from Vietnam was also detected in peninsular Malaysia.
    MeSH terms: Animals; Myanmar; Haplotypes; Indonesia; Malaysia; Nucleotides; Perciformes; Philippines; Thailand; Genetic Variation; Vietnam; Founder Effect
  19. Bahuguna A, Singh A
    Mitochondrial DNA B Resour, 2019 Oct 17;4(2):3085-3091.
    PMID: 33365866 DOI: 10.1080/23802359.2019.1667270
    Oriental giant squirrels are tree squirrels classified under family Ratufinae. In India, there are three species of genus Ratufa, i.e. Ratufa bicolor, Ratufa macroura and Ratufa indica. They are also distributed in South and Southeast Asia. However Ratufa indica is endemic to India. The fourth species Ratufa affinis is restricted to Maritime Southeast Asia (East Malaysia, Indonesia, Brunei and Thailand) and probably in Singapore. The species is near threatened .The species R.macroura is endemic to South Asia. Forests of South and Southeast Asia are hotspots of squirrel diversity but at the same time they are at a high risk of extinction because of high deforestation rate and habitat fragmentation. The present molecular study is the first study of the species of Ratufa for their identification. In this study old taxidermy samples were used for amplification of 12SrRNA and Cytochrome b genes. Maximum likelihood and neighbor-joining methods were used to delineate the species by using MEGA 6 and also for molecular analysis for variable, conserved, parsimony and singleton sites. Similarities between species through BLAST indicated 92.21-89.57% between R.macroura vs. R. bicolour; 93.22-90% Ratufa macroura vs. Ratufa indica; 96-92% Ratufa indica vs. Ratufa bicolour, 93.88% between Ratufa affinis vs. Ratufa indica, 93.5% R. affinis vs. R. bicolor, 90.5%. R. affinis vs. R. macroura. Ratufa bicolor is noted to be genetically closer to R. indica as inferred by using both markers. The BLAST result indicated that the obtained sequences matched 99-100% with their respective species. It was also noted that R. bicolor of Jalpaiguri, West Bengal are genetically closer to that of Bhutan. The study also revealed the evolution of R. indica and R. macroura from a single population.
    MeSH terms: Animals; Asia, Southeastern; Bhutan; Brunei; Conservation of Natural Resources; India; Indonesia; Malaysia; Sciuridae; Singapore; Thailand; Ecosystem; Cytochromes b; Forests
  20. Iqbal F, Ayub Q, Song BK, Wilson R, Fahim M, Rahman S
    Mitochondrial DNA B Resour, 2019 Dec 18;5(1):348-350.
    PMID: 33366551 DOI: 10.1080/23802359.2019.1704637
    Corvus macrorhynchos formerly referred to as the jungle crow or the large-billed crow is a polytypic species with unresolved taxonomy, comprising various subspecies widespread across South, Southeast, and East Asia. In this study, we report the complete mitogenome of one of these subspecies, Corvus macrorhynchos intermedius (Himalaya crow), from Pakistan. The mitochondrial genome is circular, 16,927 bp and contains typical animal mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNA) and one non-coding region (D-loop) with a nucleotide content of A (30.6%), T (24.8%), G (14.8%), and C (29.8%). Phylogenetic analysis using the whole mitochondrial genome showed that C. m. intermedius and only reported subspecies Corvus macrorhynchos culminatus (Indian Jungle crow) are genetically distinct and it supports the recognition of the latter as a separate biospecies.
    MeSH terms: Animals; Far East; Humans; Nucleotides; Pakistan; Phylogeny; RNA, Ribosomal; RNA, Transfer; Asian Continental Ancestry Group; Crows; Genes, Mitochondrial; Genome, Mitochondrial
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