Browse publications by year: 2023

Contour Analysis of Three-Dimensional Peri-Implant Mucosal Model as an Endpoint Analysis of Photofunctionalization Effects on Implant Abutment Materials

Razali M, Chai WL, Omar RA, Ngeow WC

Materials (Basel), 2023 Aug 09;16(16).
PMID: 37629819 DOI: 10.3390/ma16165529

INTRODUCTION: The objective of this study was to examine the effect of photofunctionalization on the soft-tissue contour formed at the interface of various abutment materials using end-point analyses obtained from the three-dimensional oral mucosal model (3D-OMMs).
METHODS: Commercially pure titanium (CPTi), alumina-toughened zirconia (ATZ), and yttria-stabilized zirconia (YSZ) made into discs shapes were classified into two groups: UV-treated (PTx) and non-treated (NTx). The materials in PTx groups were exposed to UV light for 12 min. Human gingival fibroblasts and TR146 epithelial cell lines co-cultured on the acellular dermal membrane were used to construct the 3D-OMM. After 4 days of culture, the discs were inserted into the holes prepared within the membrane of 3D-OMMs. The contour formed by the tissue was evaluated after 14 days of culture.
RESULTS: The UV treatment of abutment materials resulted in the formation of more non-pocket-tissue types among the PTx group (p = 0.002). Of all materials tested, soft tissue contour around YSZ showed higher scores for the non-pocket type in both non- and UV-treated groups.
CONCLUSIONS: The non-pocket type of tissue attachment was frequently found in all surfaces modified by photofunctionalization, particularly zirconia. The 3D-OMM can be used to evaluate the biological endpoints of implant surface modifications.
Properties of Ramie (Boehmeria nivea (L.) Gaudich) Fibers Impregnated with Non-Isocyanate Polyurethane Resins Derived from Lignin

Raditya VYA, Lubis MAR, Sari RK, Antov P, Lee SH, Kristak L, et al.

Materials (Basel), 2023 Aug 20;16(16).
PMID: 37629995 DOI: 10.3390/ma16165704

The textile industries need an alternative to cotton since its supply is unable to keep up with the growing global demand. The ramie (Boehmeria nivea (L.) Gaudich) fiber has a lot of potential as a renewable raw material but has low fire-resistance, which should be improved. In this work, the objectives were to investigate the characteristics of lignin derived from black liquor of kraft pulping, as well as the properties of the developed lignin-based non-isocyanate-polyurethane (L-NIPU), and to analyze ramie fiber before and after impregnation with L-NIPU. Two different formulations of L-NIPU were impregnated into ramie fiber for 30, 60, and 90 min at 25 × 2 °C under 50 kPa. The calculation of the Weight Percent Gain (WPG), Fourier Transform Infrared Spectrometer (FTIR), Rotational Rheometer, Dynamic Mechanical Analyzer (DMA), Pyrolysis Gas Chromatography Mass Spectrometer (Py-GCMS), Universal Testing Machine (UTM), and hydrolysis test were used to evaluate the properties of ramie fibers. The result showed that ramie fiber impregnated with L-NIPU produced higher mechanical property values and WPG than non-impregnated ramie fiber. There is a tendency that the longer impregnation time results in better WPG values, FTIR intensity of the urethane group, thermomechanical properties, crystallinity, and mechanical properties of ramie fiber. However, the use of DMC and HMT cannot replace the role of isocyanates in the synthesis of L-NIPU because it produces lower heat resistance than ramie impregnated using pMDI. Based on the results obtained, the impregnation of ramie fiber with L-NIPU represents a promising approach to increase its wider industrial application as a functional material.
Design and Analysis of Modified U-Shaped Four Element MIMO Antenna for Dual-Band 5G Millimeter Wave Applications

Jetti CR, Addepalli T, Devireddy SR, Tanimki GK, Al-Gburi AJA, Zakaria Z, et al.

Micromachines (Basel), 2023 Jul 31;14(8).
PMID: 37630079 DOI: 10.3390/mi14081545

A novel compact-slotted four element multiple input multiple output (MIMO) planar monopole antenna is proposed for 5G mmWave N257/N258 and N262 band applications. The antenna, with dimensions of 12 mm × 11.6 mm × 0.508 mm (1.036λo ×1.001λo×0.043λo where λo is computed at lowest cutoff frequency), is fabricated on a Rogers RT/duroid 5880 (tm) substrate with a relative permittivity of 2.2 and a dielectric loss tangent of 0.0009. The suggested antenna consists of four U-shaped radiating elements (patches) on top of the dielectric material and a slotted ground on the bottom. The radiating elements are fed by a 50-ohm microstrip line feed. To improve the impedance performance of the MIMO antenna, a rectangular strip of 1.3 mm × 0.2 mm and a couple of rectangular slots are added to each radiating element. The first operating band at 27.1 GHz, ranging from 25.9 GHz to 27.8 GHz, is achieved by using slotted U-shaped radiating elements. The second operating band at 48.7 GHz, ranging from 47.1 GHz to 49.9 GHz, is obtained by etching hexagonal slots on the ground. The antenna design achieves an isolation of >27 dB through the orthogonal positioning of radiating elements and slots on the ground. The designed antenna operates at 27 GHz (N257/N258) and 48.7 GHz (N262) bands, exhibiting stable radiation patterns, a peak gain of >5.95 dBi, radiation efficiency of >90%, an envelope correlation coefficient of <10-6, a total active reflection coefficient of ≤-10 dB, channel capacity losses of <0.03 bits/s/Hz, and a mean effective gain of ≤-3 dB. The simulated and measured results of the antenna show good agreement, making it well-suited for 5G mmWave communication applications.
Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study

Samad MIA, Ponnuthurai DR, Badrudin SI, Ali MAM, Razak MAA, Buyong MR, et al.

Micromachines (Basel), 2023 Aug 17;14(8).
PMID: 37630162 DOI: 10.3390/mi14081625

Dielectrophoresis (DEP) is one of the microfluid-based techniques that can manipulate the red blood cells (RBC) for blood plasma separation, which is used in many medical screening/diagnosis applications. The tapered aluminium microelectrode array (TAMA) is fabricated for potential sensitivity enhancement of RBC manipulation in lateral and vertical directions. In this paper, the migration properties of dielectrophoretically manipulated RBC in TAMA platform are studied at different peak-to-peak voltage (Vpp) and duration supplied onto the microelectrodes. Positive DEP manipulation is conducted at 440 kHz with the RBC of 4.00 ± 0.2 µm average radius attracted to the higher electric field intensity regions, which are the microelectrodes. High percentage of RBC migration occurred at longer manipulation time and high electrode voltage. During DEP manipulation, the RBC are postulated to levitate upwards, experience the electro-orientation mechanism and form the pearl chains before migrating to the electrodes. The presence of external forces other than the dielectrophoretic force may also affect the migration response of RBC. The safe operating limit of 10 Vpp and manipulation duration of ≤50 s prevent RBC rupture while providing high migration percentage. It is crucial to define the safe working region for TAMA devices that manipulate small RBC volume (~10 µL).
Fulltext Analysis of Pork in Beef Sausages Using LC-Orbitrap HRMS Untargeted Metabolomics Combined with Chemometrics for Halal Authentication Study

Windarsih A, Bakar NKA, Dachriyanus, Yuliana ND, Riswanto FDO, Rohman A

Molecules, 2023 Aug 09;28(16).
PMID: 37630216 DOI: 10.3390/molecules28165964

Beef sausage (BS) is one of the most favored meat products due to its nutrition and good taste. However, for economic purposes, BS is often adulterated with pork by unethical players. Pork consumption is strictly prohibited for religions including Islam and Judaism. Therefore, advanced detection methods are highly required to warrant the halal authenticity of BS. This research aimed to develop a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method to determine the halal authenticity of BS using an untargeted metabolomics approach. LC-HRMS was capable of detecting various metabolites in BS and BS containing pork. The presence of pork in BS could be differentiated using principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) with high accuracy. PLS-DA perfectly classified authentic BS and BS containing pork in all concentration levels of pork with R2X = (0.821), R2Y(= 0.984), and Q2 = (0.795). The level of pork in BS was successfully predicted through partial least squares (PLS) and orthogonal PLS (OPLS) chemometrics. Both models gave high R2 (>0.99) actual and predicted values as well as few errors, indicating good accuracy and precision. Identification of discriminating metabolites' potential as biomarker candidates through variable importance for projections (VIP) value revealed metabolites of 2-arachidonyl-sn-glycero-3-phosphoethanolamine, 3-hydroxyoctanoylcarnitine, 8Z,11Z,14Z-eicosatrienoic acid, D-(+)-galactose, oleamide, 3-hydroxyhexadecanoylcarnitine, arachidonic acid, and α-eleostearic acid as good indicators to detect pork. It can be concluded that LC-HRMS metabolomics combined with PCA, PLS-DA, PLS, and OPLS was successfully used to detect pork adulteration in beef sausages. The results imply that LC-HRMS untargeted metabolomics in combination with chemometrics is a promising alternative as an analytical technique to detect pork in sausage products. Further analysis of larger samples is required to warrant the reproducibility.

MeSH terms: Red Meat*; Animals; Cattle; Meat Products*; Swine; Reproducibility of Results; Metabolomics
Fulltext Functional and Bioactive Properties of Wheat Protein Fractions: Impact of Digestive Enzymes on Antioxidant, α-Amylase, and Angiotensin-Converting Enzyme Inhibition Potential

Gammoh S, Alu'datt MH, Alhamad MN, Tranchant CC, Rababah T, Al-U'datt D, et al.

Molecules, 2023 Aug 11;28(16).
PMID: 37630264 DOI: 10.3390/molecules28166012

This research aimed to determine the biofunctional properties of wheat flour (WF) protein fractions and modifications to the antioxidant, anti-α-amylase and anti-angiotensin-I converting enzyme (ACE) activities induced by the action of digestive endopeptidases in vitro. A molecular characterization of the most abundant protein fractions, i.e., albumins, glutelins-1, glutelins-2 and prolamins, showed that low- and high-MW polypeptides rich in cysteine, glutamic acid and leucine were present in albumins and glutelins, whereas low-MW subunits with a high proportion of polar amino acids prevailed in prolamins. Prolamins exhibited the second-highest water holding capacity (54%) after WF (84%), while albumins provided superior foam stability (76%). Prolamins, glutenins-1 and globulins demonstrated the highest antioxidant activity (up to 95%, 68% and 59%, respectively) both before and after hydrolysis with pepsin (P-H) or trypsin-chymotrypsin (TC-H). Prolamins, globulins and WF strongly inhibited α-amylase (>90%) before and after TC-H, and before P-H (55-71%). Moreover, P-H significantly increased α-amylase inhibition by albumins from 53 to 74%. The fractions with strong ACE inhibitory activity (70-89%) included prolamins and globulins after TC-H or P-H, as well as globulins before TC-H and WF before P-H. This novel evidence indicates that WF protein fractions and their peptide-enriched P and TC hydrolysates are excellent sources of multifunctional bioactives with antioxidant, antihyperglycemic and antihypertensive potential.

MeSH terms: Albumins; alpha-Amylases; Flour; Gastrointestinal Agents; Glutens; Triticum*
Fulltext Antimicrobial Potential of Aqueous Extract of Giant Sword Fern and Ultra-High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry Analysis

Venmathi Maran BA, Palaniveloo K, Mahendran T, Chellappan DK, Tan JK, Yong YS, et al.

Molecules, 2023 Aug 15;28(16).
PMID: 37630329 DOI: 10.3390/molecules28166075

Vibriosis and parasitic leech infestations cause the death of various farmed fish, such as groupers, hybrid groupers, sea bass, etc., in Malaysia and other Southeast Asian countries. In the absence of natural control agents, aquaculture operators rely on toxic chemicals to control Vibrio infections and parasitic leeches, which can have a negative impact on the environment and health. In the present study, we investigated the antivibrio and antiparasitic activities of the aqueous extract of giant sword fern (GSF) (Nephrolepis biserrata, Nephrolepidaceae, locally known as "Paku Pedang") against four Vibrio spp. and the parasitic leech Zeylanicobdella arugamensis, as well as its metabolic composition using the ultra-high-performance liquid chromatography-high-resolution mass spectrometry system (UHPLC-HRMS). The data show that the aqueous extract of GSF at a concentration of 100 mg/mL exhibits potent bactericidal activity against V. parahaemolyticus with a zone of inhibition of 19.5 mm. In addition, the extract showed dose-dependent activity against leeches, resulting in the complete killing of the parasitic leeches within a short period of 11-43 min when tested at concentrations ranging from 100 to 25 mg/mL. The UHPLC-HRMS analysis detected 118 metabolites in the aqueous extract of GSF. Flavonoids were the primary metabolites, followed by phenolic, aromatic, fatty acyl, terpenoid, vitamin and steroidal compounds. Notably, several of these metabolites possess antibacterial and antiparasitic properties, including cinnamaldehyde, cinnamic acid, apigenin, quercetin, cynaroside, luteolin, naringenin, wogonin, 6-gingerol, nicotinamide, abscisic acid, daidzein, salvianolic acid B, etc. Overall, our study shows the significant antibacterial and antiparasitic potential of the GSF aqueous extract, which demonstrates the presence of valuable secondary metabolites. Consequently, the aqueous extract is a promising natural alternative for the effective control of Vibrio infections and the treatment of parasitic leeches in aquaculture systems.

MeSH terms: Animals; Anti-Bacterial Agents/pharmacology; Antiparasitic Agents/pharmacology; Chromatography, High Pressure Liquid; Ferns*
Fulltext Ternary Solid Dispersions: A Review of the Preparation, Characterization, Mechanism of Drug Release, and Physical Stability

Budiman A, Lailasari E, Nurani NV, Yunita EN, Anastasya G, Aulia RN, et al.

Pharmaceutics, 2023 Aug 10;15(8).
PMID: 37631330 DOI: 10.3390/pharmaceutics15082116

The prevalence of active pharmaceutical ingredients (APIs) with low water solubility has experienced a significant increase in recent years. These APIs present challenges in formulation, particularly for oral dosage forms, despite their considerable therapeutic potential. Therefore, the improvement of solubility has become a major concern for pharmaceutical enterprises to increase the bioavailability of APIs. A promising formulation approach that can effectively improve the dissolution profile and the bioavailability of poorly water-soluble drugs is the utilization of amorphous systems. Numerous formulation methods have been developed to enhance poorly water-soluble drugs through amorphization systems, including co-amorphous formulations, amorphous solid dispersions (ASDs), and the use of mesoporous silica as a carrier. Furthermore, the successful enhancement of certain drugs with poor aqueous solubility through amorphization has led to their incorporation into various commercially available preparations, such as ASDs, where the crystalline structure of APIs is transformed into an amorphous state within a hydrophilic matrix. A novel approach, known as ternary solid dispersions (TSDs), has emerged to address the solubility and bioavailability challenges associated with amorphous drugs. Meanwhile, the introduction of a third component in the ASD and co-amorphous systems has demonstrated the potential to improve performance in terms of solubility, physical stability, and processability. This comprehensive review discusses the preparation and characterization of poorly water-soluble drugs in ternary solid dispersions and their mechanisms of drug release and physical stability.
Performances of Polymer-Dispersed Liquid Crystal Films for Smart Glass Applications

Islam MS, Chan KY, Thien GSH, Low PL, Lee CL, Wong SK, et al.

Polymers (Basel), 2023 Aug 16;15(16).
PMID: 37631477 DOI: 10.3390/polym15163420

Polymer-dispersed liquid crystal (PDLC) film is an active smart film penetrating the market due to its unique functionalities. These functional characteristics include switchable tint capabilities, which shield building residents from the sun's harmful ultraviolet (UV) rays, improve energy-saving features, and produce higher cost-efficiency. Although PDLC films are promising in several applications, there is still ambiguity on the performance of PDLC films. Particularly, the sizing effects' (such as film thickness and area) correlation with visible light transmission (VLT), ultraviolet rejection (UVR), infrared rejection (IRR), light intensity, current consumption, and apparent power consumption is not well understood. Therefore, this study investigated the sizing effects of PDLC films, including the thickness effect on VLT, UVR, IRR, light intensity, and area influence on current and apparent power consumptions. The varying applied voltage effect on the light transmittance of the PDLC film was also effectively demonstrated. A 0.1 mm PDLC film was successfully presented as a cost-efficient film with optimal parameters. Consequently, this study paves the way for a clearer understanding of PDLC films (behavior and sizing effects) in implementing economic PDLC films for large-scale adoption in commercial and residential premises.
Tensile Properties of In Situ 3D Printed Glass Fiber-Reinforced PLA

Ismail KI, Pang R, Ahmed R, Yap TC

Polymers (Basel), 2023 Aug 17;15(16).
PMID: 37631493 DOI: 10.3390/polym15163436

A 3D printed composite via the fused filament fabrication (FFF) technique has potential to enhance the mechanical properties of FFF 3D printed parts. The most commonly employed techniques for 3D composite printing (method 1) utilized premixed composite filaments, where the fibers were integrated into thermoplastic materials prior to printing. In the second method (method 2), short fibers and thermoplastic were mixed together within the extruder of a 3D printer to form a composite part. However, no research has been conducted on method 3, which involves embedding short fibers into the printed object during the actual printing process. A novel approach concerning 3D printing in situ fiber-reinforced polymer (FRP) by embedding glass fibers between deposited layers during printing was proposed recently. An experimental investigation has been undertaken to evaluate the tensile behavior of the composites manufactured by the new manufacturing method. Neat polylactic acid (PLA) and three different glass fiber-reinforced polylactic acid (GFPLA) composites with 1.02%, 2.39%, and 4.98% glass fiber contents, respectively, were 3Dprinted. Tensile tests were conducted with five repetitions for each sample. The fracture surfaces of the samples were then observed under scanning electron microscopy (SEM). In addition, the porosities of the 3D printed samples were measured with a image processing software (ImageJ 1.53t). The result shows that the tensile strengths of GFPLA were higher than the neat PLA. The tensile strength of the composites increased from GFPLA-1 (with a 1.02% glass fiber content) to GFPLA-2.4 (with a 2.39% glass fiber content), but drastically dropped at GFPLA-5 (with a 4.98% glass fiber content). However, the tensile strength of GFPLA-5 is still higher than the neat PLA. The fracture surfaces of tensile samples were observed under scanning electron microscopy (SEM). The SEM images showed the average line width of the deposited material increased as glass fiber content increased, while layer height was maintained. The intralayer bond of the deposited filaments improved via the new fiber embedding method. Hence, the porosity area is reduced as glass fiber content increased.
Sustainable Resource Allocation and Reduce Latency Based on Federated-Learning-Enabled Digital Twin in IoT Devices

Alhartomi MA, Salh A, Audah L, Alzahrani S, Alzahmi A, Altimania MR, et al.

Sensors (Basel), 2023 Aug 18;23(16).
PMID: 37631798 DOI: 10.3390/s23167262

In this article, we utilize Digital Twins (DT) with edge networks using blockchain technology for reliable real-time data processing and provide a secure, scalable solution to bridge the gap between physical edge networks and digital systems. Then, we suggest a Federated Learning (FL) framework for collaborative computing that runs on a blockchain and is powered by the DT edge network. This framework increases data privacy while enhancing system security and reliability. The provision of sustainable Resource Allocation (RA) and ensure real-time data-processing interaction between Internet of Things (IoT) devices and edge servers depends on a balance between system latency and Energy Consumption (EC) based on the proposed DT-empowered Deep Reinforcement Learning (Deep-RL) agent. The Deep-RL agent evaluates the performance action based on RA actions in DT to distribute its bandwidth resources to IoT devices based on iteration and the actions taken to generate the best policy and enhance learning efficiency at every step. The simulation results show that the proposed Deep-RL-agent-based DT is able to exploit the best policy, select 47.5% of computing activities that are to be carried out locally with 1 MHz bandwidth and minimize the weighted cost of the transmission policy of edge-computing strategies.
Acceptance of a Text Messaging Vaccination Reminder and Recall System in Malaysia's Healthcare Sector: Extending the Technology Acceptance Model

Karkonasasi K, Cheah YN, Vadiveloo M, Mousavi SA

Vaccines (Basel), 2023 Aug 06;11(8).
PMID: 37631899 DOI: 10.3390/vaccines11081331

Malaysian healthcare institutions still use ineffective paper-based vaccination systems to manage childhood immunization schedules. This may lead to missed appointments, incomplete vaccinations, and outbreaks of preventable diseases among infants. To address this issue, a text messaging vaccination reminder and recall system named Virtual Health Connect (VHC) was studied. VHC simplifies and accelerates immunization administration for nurses, which may result in improving the completion and timeliness of immunizations among infants. Considering the limited research on the acceptance of these systems in the healthcare sector, we examined the factors influencing nurses' attitudes and intentions to use VHC using the extended technology acceptance model (TAM). The novelty of the conceptual model is the incorporation of new predictors of attitude, namely, perceived compatibility and perceived privacy and security issues. We conducted a survey among 121 nurses in Malaysian government hospitals and clinics to test the model. We analyzed the collected data using partial least squares structural equation modeling (PLS-SEM) to examine the significant factors influencing nurses' attitudes and intentions to use VHC. Moreover, we applied an artificial neural network (ANN) to determine the most significant factors of acceptance with higher accuracy. Therefore, we could offer more accurate insights to decision-makers in the healthcare sector for the advancement of health services. Our results highlighted that the compatibility of VHC with the current work setting of nurses developed their positive perspectives on the system. Moreover, the nurses felt optimistic about the system when they considered it useful and easy to use in the workplace. Finally, their attitude toward using VHC played a pivotal role in increasing their intention to use it. Based on the ANN models, we also found that perceived compatibility was the most significant factor influencing nurses' attitudes towards using VHC, followed by perceived ease of use and perceived usefulness.
Substitution of Coxsackievirus A16 VP1 BC and EF Loop Altered the Protective Immune Responses in Chimera Enterovirus A71

Tan XH, Chong WL, Lee VS, Abdullah S, Jasni K, Suarni SQ, et al.

Vaccines (Basel), 2023 Aug 14;11(8).
PMID: 37631931 DOI: 10.3390/vaccines11081363

Hand, foot and mouth disease (HFMD) is a childhood disease caused by enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16). Capsid loops are important epitopes for EV-A71 and CV-A16. Seven chimeric EV-A71 (ChiE71) involving VP1 BC (45.5% similarity), DE, EF, GH and HI loops, VP2 EF loop and VP3 GH loop (91.3% similarity) were substituted with corresponding CV-A16 loops. Only ChiE71-1-BC, ChiE71-1-EF, ChiE71-1-GH and ChiE71-3-GH were viable. EV-A71 and CV-A16 antiserum neutralized ChiE71-1-BC and ChiE71-1-EF. Mice immunized with inactivated ChiE71 elicited high IgG, IFN-γ, IL-2, IL-4 and IL-10. Neonatal mice receiving passive transfer of WT EV-A71, ChiE71-1-EF and ChiE71-1-BC immune sera had 100%, 80.0% and no survival, respectively, against lethal challenges with EV-A71, suggesting that the substituted CV-A16 loops disrupted EV-A71 immunogenicity. Passive transfer of CV-A16, ChiE71-1-EF and ChiE71-1-BC immune sera provided 40.0%, 20.0% and 42.9% survival, respectively, against CV-A16. One-day-old neonatal mice immunized with WT EV-A71, ChiE71-1-BC, ChiE71-1-EF and CV-A16 achieved 62.5%, 60.0%, 57.1%, and no survival, respectively, after the EV-A71 challenge. Active immunization using CV-A16 provided full protection while WT EV-A71, ChiE71-1-BC and ChiE71-1-EF immunization showed partial cross-protection in CV-A16 lethal challenge with survival rates of 50.0%, 20.0% and 40%, respectively. Disruption of a capsid loop could affect virus immunogenicity, and future vaccine design should include conservation of the enterovirus capsid loops.
Computational evaluation of quinones of Nigella sativa L. as potential inhibitor of dengue virus NS5 methyltransferase

Roney M, Dubey A, Nasir MH, Huq AM, Tufail A, Tajuddin SN, et al.

J Biomol Struct Dyn, 2023 Aug 26.
PMID: 37632317 DOI: 10.1080/07391102.2023.2248262

Aedes aegypti is the primary vector for the transmission of the dengue virus, which causes dengue fever, dengue hemorrhagic illness and dengue shock syndrome. There is now no antiviral medication available to treat DENV, which kills thousands of people each year and infects millions of individuals. A possible target for the creation of fresh and efficient dengue treatments is the DENV-3 NS5 MTase. So, Nigella sativa quinones were examined using in silico methods to find natural anti-DENV compounds. The in silico docking was conducted utilising the Discovery Studio software on the quinones of N. sativa and the active site of the target protein DENV-3 NS5 MTase. In addition, the druggability and pharmacokinetics of the lead compound were assessed. Dithymoquinone was comparable to the reference compound in terms of its ability to bind to the active site of target protein. Dithymoquinone met the requirements for drug likeness and Lipinski's principles, as demonstrated by the ADMET analysis and drug likeness results. The current study indicated that the dithymoquinone from N. sativa had anti-DENV activity, suggesting further drug development and dengue treatment optimisation.Communicated by Ramaswamy H. Sarma.
Risk factors for mortality over 18 years in 317 ICUs in 9 Asian countries: The impact of healthcare-associated infections

Rosenthal VD, Jin Z, Rodrigues C, Myatra SN, Divatia JV, Biswas SK, et al.

Infect Control Hosp Epidemiol, 2023 Aug;44(8):1261-1266.
PMID: 36278508 DOI: 10.1017/ice.2022.245

OBJECTIVE: To identify risk factors for mortality in intensive care units (ICUs) in Asia.
DESIGN: Prospective cohort study.
SETTING: The study included 317 ICUs of 96 hospitals in 44 cities in 9 countries of Asia: China, India, Malaysia, Mongolia, Nepal, Pakistan, Philippines, Sri Lanka, Thailand, and Vietnam.
PARTICIPANTS: Patients aged >18 years admitted to ICUs.
RESULTS: In total, 157,667 patients were followed during 957,517 patient days, and 8,157 HAIs occurred. In multiple logistic regression, the following variables were associated with an increased mortality risk: central-line-associated bloodstream infection (CLABSI; aOR, 2.36; P < .0001), ventilator-associated event (VAE; aOR, 1.51; P < .0001), catheter-associated urinary tract infection (CAUTI; aOR, 1.04; P < .0001), and female sex (aOR, 1.06; P < .0001). Older age increased mortality risk by 1% per year (aOR, 1.01; P < .0001). Length of stay (LOS) increased mortality risk by 1% per bed day (aOR, 1.01; P < .0001). Central-line days increased mortality risk by 2% per central-line day (aOR, 1.02; P < .0001). Urinary catheter days increased mortality risk by 4% per urinary catheter day (aOR, 1.04; P < .0001). The highest mortality risks were associated with mechanical ventilation utilization ratio (aOR, 12.48; P < .0001), upper middle-income country (aOR, 1.09; P = .033), surgical hospitalization (aOR, 2.17; P < .0001), pediatric oncology ICU (aOR, 9.90; P < .0001), and adult oncology ICU (aOR, 4.52; P < .0001). Patients at university hospitals had the lowest mortality risk (aOR, 0.61; P < .0001).
CONCLUSIONS: Some variables associated with an increased mortality risk are unlikely to change, such as age, sex, national economy, hospitalization type, and ICU type. Some other variables can be modified, such as LOS, central-line use, urinary catheter use, and mechanical ventilation as well as and acquisition of CLABSI, VAE, or CAUTI. To reduce mortality risk, we shall focus on strategies to reduce LOS; strategies to reduce central-line, urinary catheter, and mechanical ventilation use; and HAI prevention recommendations.

MeSH terms: Adult; Child; Delivery of Health Care; Female; Hospitals, University; Humans; Intensive Care Units; Pakistan/epidemiology; Prospective Studies; Risk Factors; Urinary Tract Infections*; Pneumonia, Ventilator-Associated*
Revising and exploring the variations in methodologies for establishing the diagnostic reference levels for paediatric PET/CT imaging

Alhorani Q, Alkhybari E, Rawashdeh M, Sabarudin A, Latiff RA, Al-Ibraheem A, et al.

Nucl Med Commun, 2023 Nov 01;44(11):937-943.
PMID: 37615527 DOI: 10.1097/MNM.0000000000001748

PET-computed tomography (PET/CT) is a hybrid imaging technique that combines anatomical and functional information; to investigate primary cancers, stage tumours, and track treatment response in paediatric oncology patients. However, there is debate in the literature about whether PET/CT could increase the risk of cancer in children, as the machine is utilizing two types of radiation, and paediatric patients have faster cell division and longer life expectancy. Therefore, it is essential to minimize radiation exposure by justifying and optimizing PET/CT examinations and ensure an acceptable image quality. Establishing diagnostic reference levels (DRLs) is a crucial quantitative indicator and effective tool to optimize paediatric imaging procedures. This review aimed to distinguish and acknowledge variations among published DRLs for paediatric patients in PET/CT procedures. A search of relevant articles was conducted using databases, that is, Embase, Scopus, Web of Science, and Medline, using the keywords: PET-computed tomography, computed tomography, PET, radiopharmaceutical, DRL, and their synonyms. Only English and full-text articles were included, with no limitations on the publication year. After the screening, four articles were selected, and the review reveals different DRL approaches for paediatric patients undergoing PET/CT, with primary variations observed in patient selection criteria, reporting of radiation dose values, and PET/CT equipment. The study suggests that future DRL methods for paediatric patients should prioritize data collection in accordance with international guidelines to better understand PET/CT dose discrepancies while also striving to optimize radiation doses without compromising the quality of PET/CT images.

MeSH terms: Child; Humans; Radiation Dosage; Reference Values; Tomography, X-Ray Computed; Radiopharmaceuticals
Rice husk coated with copper oxide nanoparticles for 17α-ethinylestradiol removal from an aqueous solution: adsorption mechanisms and kinetics

Alhares HS, Ali QA, Shaban MAA, M-Ridha MJ, Bohan HR, Mohammed SJ, et al.

Environ Monit Assess, 2023 Aug 24;195(9):1078.
PMID: 37615739 DOI: 10.1007/s10661-023-11689-6

The 17 α-ethinylestradiol (EE2) adsorption from aqueous solution was examined using a novel adsorbent made from rice husk powder coated with CuO nanoparticles (CRH). Advanced analyses of FTIR, XRD, SEM, and EDSwere used to identify the classification parameters of a CRH-like surface morphology, configuration, and functional groups. The rice husk was coated with CuO nanoparticles, allowing it to create large surface area materials with significantly improved textural qualities with regard to functional use and adsorption performance, according to a detailed characterization of the synthesized materials. The adsorption process was applied successfully with elimination effectiveness of 100% which can be kept up to 61.3%. The parameters of adsorption were affecting the adsorption process significantly. Thermodynamic data stated that the process of adsorption was endothermic, spontaneous, chemisorption and the molecules of EE2 show affinity with the CRH. It was discovered that the adsorption process controlled by a pseudo-second-order kinetic model demonstrates that the chemisorption process was controlling EE2 removal. The Sips model is regarded as optimal for representing this practice, exhibiting a significantly high determination coefficient of 0.948. This coefficient implies that the adsorption mechanism indicates the occurrence of both heterogeneous and homogeneous adsorption. According to the findings, biomass can serve as a cheap, operative sorbent to remove estrogen from liquified solutions.

MeSH terms: Adsorption; Copper; Environmental Monitoring; Ethinyl Estradiol; Kinetics; Oxides; Oryza*; Nanoparticles*
Modeling the significance of green orientation and culture on green innovation performance: moderating effect of firm size and green implementation

Bhatti SM, Al Mamun A, Wu M, Naznen F, Kanwal S, Makhbul ZKM

Environ Sci Pollut Res Int, 2023 Sep;30(44):99855-99874.
PMID: 37615918 DOI: 10.1007/s11356-023-29353-4

The current global trend in sustainable business practices is to optimize green innovation performance. To protect the environment and maintain their own survival, organizations must strengthen their green innovation capabilities. Drawing on the recourse-based view and ecology modernization theory (EMT), this study examines the direct effect of green strategic orientations, green entrepreneurial orientation, green market orientation, green innovation orientation, and green organizational culture on the firm's green innovation capability, as well as the mediating effect of green innovation capability on the relationship of these four factors and green innovation performance. Besides, this study also explored the moderating effects of green management system implementation and firm size on the association between green innovation capability and green innovation performance. To test the hypothesized model, a questionnaire survey was administered to gather responses from 293 medium-sized and large manufacturing firms operating in Pakistan. The partial least squares method was used for data analysis. The results revealed that green entrepreneurial orientation, green market orientation, green innovation orientation, and green organizational culture positively impacted green innovation capability, which subsequently positively influenced green innovation performance. Moreover, effective implementation of green management systems can bolster the effect of green innovation capability on green innovation performance, and the mediating effect of green innovation capability has also been confirmed. These indicated that the management of medium and large manufacturing firms operating in Pakistan should focus on encouraging green innovation and training employees regarding the latest eco-friendly technologies to attain performance and sustainable development goals. Policymakers should implement green business development programs and offer rewards or penalties for promoting compliance. The present study contributes greatly to the literature by applying EMT as an alternative to address the mediating role of green innovation capability and the moderating effect of green management system implementation in enhancing firms' green innovation performance.

MeSH terms: Commerce*; Ecology; Humans; Pakistan; Organizational Culture
Fulltext An mHealth-Delivered Sexual Harm Reduction Tool (PartyPack) for Men Who Have Sex With Men in Malaysia: Usability Study

Gautam K, Paudel K, Jacobs J, Wickersham JA, Ikhtiaruddin WM, Azwa I, et al.

JMIR Form Res, 2023 Aug 24;7:e48113.
PMID: 37616034 DOI: 10.2196/48113

BACKGROUND: Chemsex-the use of psychoactive drugs to enhance the sexual experience-is an increasing phenomenon globally. Despite the increasing burden and associated harms of chemsex, evidence-based interventions (ie, behavioral and pharmacological) for chemsex users are nonexistent.
OBJECTIVE: In this study, we assessed the usability and acceptability of a mobile health (mHealth)-delivered safer chemsex package ("PartyPack") as a sexual harm reduction strategy among men who have sex with men in Malaysia-a setting where chemsex is becoming increasingly prevalent.
METHODS: This study is part of a larger smartphone app-based intervention (ie, JomPrEP; University of Connecticut) designed to improve access to HIV prevention services among Malaysian men who have sex with men. A total of 50 participants were recruited from the Greater Kuala Lumpur region of Malaysia to use the JomPrEP app, which included a feature allowing participants to order PartyPack, for 30 days (March-April 2022). The usability and acceptability of the PartyPack were assessed using self-report, app analytics, and exit interviews (n=20).
RESULTS: Overall, 8% (4/50) of participants reported having engaged in chemsex in the past 6 months; however, engagement in condomless sex (34/50, 68%) and group sex (9/50, 18%) was much higher. A total of 43 (86%) participants ordered PartyPack, of which 27 (63%) made multiple orders during the 30 days. Most participants (41/43, 95%) reported being satisfied with the PartyPack order feature in the app, with 91% (39/43) indicating the order and tracking process was easy. Thematic data exploration further revealed important information for understanding (eg, items included in the package, use of mHealth platform to order package, and discreetness of the PartyPack box and order and delivery) and refining the logistical preferences (eg, using branded items and allowing customization during order).
CONCLUSIONS: Our findings provide strong evidence of the usability and acceptability of a mHealth-delivered safer chemsex package as a potential sexual harm reduction tool among this underserved population. Replication in a study with a larger sample size to test the efficacy of the PartyPack is warranted.
Fulltext Systematic review on factors associated with depression among mothers of children with cancer

Wan Ghazali WS, Minhat HS, Mohd Zulkefli NA, Ahmad N, Ismail F, Mashudi DN, et al.

PLoS One, 2023;18(8):e0285366.
PMID: 37616287 DOI: 10.1371/journal.pone.0285366

BACKGROUND: Despite evidence of depression among mothers of children with cancer, there appears to be a lack of studies or concern regarding factors associated with depression among these mothers.
OBJECTIVE: To review the factors associated with depression among mothers of children with cancer.
METHOD: Pubmed, Medline, Cochrane, CINAHL, Psychology, and Behavioural Sciences Collection, and Academic Search Complete were searched according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines to identify studies published between 2010 to 2022 on the associated risk factors of depression among mothers of children with cancer. The keywords used included mothers OR maternal' AND 'Child*' AND 'cancer OR tumo*r OR neoplasm' AND 'factors OR facilitators AND barriers OR predictors OR determinants AND 'depression'. Selected studies were evaluated by quality assessment.
RESULT: Five articles fulfilled the eligibility criteria. The factors associated with depression among mothers of children were socio-demographic risk factors (marital status, education level, annual income, child cancer diagnosis), and stress factors (caregiving stress, cancer-related stress, general stress). There were other factors associated with depression that act as mediators along the process which were emotion-focused coping and perceived social support.
CONCLUSION: Besides the commonly reported socio-demographic risk factors (marital status, education level, and annual income), other factors include stress factors (caregiving stress, cancer-related stress, and general stress). Furthermore, emotion-focused coping and perceived social support act as mediators along the process. More studies are warranted to explore depression among these mothers to ensure the most appropriate and effective preventive measures.

MeSH terms: Adaptation, Psychological; Behavioral Sciences*; Child; Educational Status; Female; Humans; Mothers

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