Browse publications by year: 2023

  1. Chimonyo VGP, Govender L, Nyathi M, Scheelbeek PFD, Choruma DJ, Mustafa M, et al.
    Front Nutr, 2023;10:1060246.
    PMID: 36793925 DOI: 10.3389/fnut.2023.1060246
    INTRODUCTION: Intercropping cereals with legumes can intensify rainfed cereal monocropping for improved household food and nutritional security. However, there is scant literature confirming the associated nutritional benefits.

    METHODOLOGY: A systematic review and meta-analysis of nutritional water productivity (NWP) and nutrient contribution (NC) of selected cereal-legume intercrop systems was conducted through literature searches in Scopus, Web of Science and ScienceDirect databases. After the assessment, only nine articles written in English that were field experiments comprising grain cereal and legume intercrop systems were retained. Using the R statistical software (version 3.6.0), paired t-tests were used to determine if differences existed between the intercrop system and the corresponding cereal monocrop for yield (Y), water productivity (WP), NC, and NWP.

    RESULTS: The intercropped cereal or legume yield was 10 to 35% lower than that for the corresponding monocrop system. In most instances, intercropping cereals with legumes improved NY, NWP, and NC due to their added nutrients. Substantial improvements were observed for calcium (Ca), where NY, NWP, and NC improved by 658, 82, and 256%, respectively.

    DISCUSSION: Results showed that cereal-legume intercrop systems could improve nutrient yield in water-limited environments. Promoting cereal- legume intercrops that feature nutrient-dense legume component crops could contribute toward addressing the SDGs of Zero Hunger (SDG 3), Good Health and Well-3 (SDG 2) and Responsible consumption and production (SDG 12).

  2. Manoharan S, Ying LY
    Int J Clin Pract, 2023;2023:1068000.
    PMID: 36793928 DOI: 10.1155/2023/1068000
    Epstein-Barr virus (EBV) reactivation in acute-phase of COVID-19 disease was recently discovered but it is not clear in terms of degree of mortality caused, and this was the aim of the current study. Six databases and three non-databases were thoroughly searched, independently. The articles related to non-human study (abstract, in vitro, in vivo, in silico, case study, poster, and review articles) were excluded for main analysis. Four articles related to mortality linked to EBV reactivation were systematically identified and included in the qualitative and quantitative analyses. Based on proportional meta-analysis of 4 studies, 34.3% or 0.343 (95% CI: 0.189-0.516; I 2 = 74.6) mortality related to EBV reactivation was identified. To address high heterogeneity, subgroup meta-analysis was carried out. Based on subgroup analysis, 26.6% or 0.266 (95% CI: 0.191-0.348; I 2 = 0) with no heterogeneity was identified. Interestingly, in comparative meta-analysis, EBV(-)/SARS-CoV-2(+) patients had statistically lesser mortality (9.9%) than EBV(+)/SARS-CoV-2(+) patients (23.6%) where RR = 2.31 (95% CI: 1.34-3.99; p = 0.003; I 2 = 6%). This finding is equivalent to the absolute mortality effect of 130 more per 1000 COVID-19 patients (95% CI: 34-296). Furthermore, based on statistical analysis, D-dimer was not statistically significantly different (p > 0.05) between the groups although studies have shown that D-dimer was statistically significantly different (p < 0.05) between these groups. Based on the inclusion and analysis of low risk of bias and high quality of articles graded with Newcastle-Ottawa Scale (NOS), when COVID-19 patients' health state is gradually worsening, EBV reactivation needs to be suspected because EBV reactivation is a possible marker for COVID-19 disease severity.
    MeSH terms: Herpesvirus 4, Human/physiology; Hospitalization; Humans
  3. Kumar P, Islam MA, Pillai R, Sharif T
    Heliyon, 2023 Feb;9(2):e13085.
    PMID: 36793953 DOI: 10.1016/j.heliyon.2023.e13085
    Adding to the behavioural science domain, the principal idea behind the study is to investigate the impact of an array of behavioural, psychological, and demographic factors on financial decision making. The study utilizes a structured questionnaire to collect the opinions of 634 investors using a blend of random and snowball sampling techniques. The partial least squares structural equation modelling has been used to test hypotheses. PLS Predict has been applied to estimate the out-of-sample predictive power of the proposed model. Finally, the multi-group analysis has been applied to assess the differences across gender. Our findings attest the relevance of digital financial literacy, financial capability, financial autonomy, and impulsivity on financial decision making. Additionally, financial capability partially mediates the nexus between digital financial literacy and financial decision making. Also, Impulsivity negatively moderates the relationship between financial capability and financial decision making. The overall results of this comprehensive and unique study portray the influence that various psychological, behavioural, and demographic factors have on financial decision making, favouring the design of a feasible and lucrative financial portfolio to ensure financial well-being of households in the long run.
  4. Abid MB, Wahab RA, Salam MA, Gzara L, Moujdin IA
    Heliyon, 2023 Feb;9(2):e12810.
    PMID: 36793956 DOI: 10.1016/j.heliyon.2023.e12810
    Water is a critical component for humans to survive, especially in arid lands or areas where fresh water is scarce. Hence, desalination is an excellent way to effectuate the increasing water demand. Membrane distillation (MD) technology entails a membrane-based non-isothermal prominent process used in various applications, for instance, water treatment and desalination. It is operable at low temperature and pressure, from which the heat demand for the process can be sustainably sourced from renewable solar energy and waste heat. In MD, the water vapors are gone through the membrane's pores and condense at permeate side, rejecting dissolved salts and non-volatile substances. However, the efficacy of water and biofouling are the main challenges for MD due to the lack of appropriate and versatile membrane. Numerous researchers have explored different membrane composites to overcome the above-said issue, and attempt to develop efficient, elegant, and biofouling-resistant novel membranes for MD. This review article addresses the 21st-century water crises, desalination technologies, principles of MD, the different properties of membrane composites alongside compositions and modules of membranes. The desired membrane characteristics, MD configurations, role of electrospinning in MD, characteristics and modifications of membranes used for MD are also highlighted in this review.
  5. Binti Seruji NMU, Rosli MM, Jong VYM, Karunakaran T, Lizazman MA, Marlina A, et al.
    IUCrdata, 2023 Jan;8(Pt 1):x221198.
    PMID: 36794049 DOI: 10.1107/S2414314622011981
    The title compound (trivial name brasixanthone B), C23H22O5, isolated from Calophyllum gracilentum, is characterized by a xanthone skeleton of three fused six-membered rings plus an additional fused pyrano ring and one 3-methyl-but-2-enyl side chain. The core xanthone moiety is almost planar, with a maximum deviation 0.057 (4) Å from the mean plane. In the mol-ecule, an intra-molecular O-H⋯O hydrogen bond forms an S(6) ring motif. The crystal structure features inter-molecular O-H⋯O and C-H⋯O inter-actions.
  6. Khan A, Khan S, Khan MA, Zaman K, Khan HUR, Rosman ASB, et al.
    Front Public Health, 2023;11:1067940.
    PMID: 36794076 DOI: 10.3389/fpubh.2023.1067940
    MeSH terms: India; Occupations*; Socioeconomic Factors
  7. Tuang GJ, Zahedi FD, Husain S, Hamizan AKW, Kew TY, Thanabalan J
    Int J Med Sci, 2023;20(2):211-218.
    PMID: 36794158 DOI: 10.7150/ijms.68095
    Introduction: The fundament of forensic science lies in identifying a body. The morphological complexity of the paranasal sinus (PNS), which varies greatly amongst individual, possess a discriminatory value that potentially contributes to the radiological identification. The sphenoid bone represents the keystone of the skull and forms part of the cranial vault. It is intimately associated with vital neurovascular structures. The sphenoid sinus, located within the body of the sphenoid bone, has variable morphology. The sphenoid septum's inconsistent position and the degree, as well as the direction disparities of sinus pneumatization, have indeed accorded it a unique structure in providing invaluable information in forensic personnel identification. Additionally, the sphenoid sinus is situated deep within the sphenoid bone. Therefore, it is well protected from traumatic degradation from external causes and can be potentially utilized in forensic studies. The authors aim to study the possibility of variation among the race, and gender in the Southeast Asian (SEA) population, using volumetric measurements of the sphenoid sinus. Materials and methods: This is a retrospective cross-sectional analysis of computerized tomographic (CT) imaging of the PNS of 304 patients (167 males, 137 females) in a single centre. The volume of the sphenoid sinus was reconstructed and measured using commercial real-time segmentation software. Result: The total volume of sphenoid sinus of male gender had shown to be larger, 12.22 (4.93 - 21.09) cm3 compared to the counterpart of 10.19 (3.75 - 18.72) cm3 (p = .0090). The Chinese possessed a larger total sphenoid sinus volume, 12.96 (4.62 - 22.21) cm3) than the Malays, 10.68 (4.13 - 19.25) cm3 (p = .0057). No correlation was identified between the age and volume of the sinus (cc= -.026, p = .6559). Conclusion: The sphenoid sinus volume in males was found to be larger than those of females. It was also shown that race influences sinus volume. Volumetric analysis of the sphenoid sinus can potentially be utilized in gender and race determination. The current study provided normative data on the sphenoid sinus volume in the SEA region, which can be helpful for future studies.
    MeSH terms: Cross-Sectional Studies; Female; Humans; Male; Retrospective Studies; Tomography, X-Ray Computed/methods
  8. Sornillo JB, Ditangco R, Lumbiganon P, Vu TA, Le ON, Truong KH, et al.
    AIDS Care, 2023 Dec;35(12):1928-1937.
    PMID: 36794343 DOI: 10.1080/09540121.2023.2176424
    Disclosure of HIV status is an important part of pediatric care. We studied disclosure and clinical outcomes in a multi-country Asian cohort of children and adolescents with HIV. Those 6-19 years of age who initiated combination antiretroviral therapy (cART) between 2008 and 2018, and who had at least one follow-up clinic visit were included. Data up to December 2019 were analyzed. Cox and competing risk regression analyses were used to assess the effect of disclosure on disease progression (WHO clinical stage 3 or 4), loss to follow-up (LTFU; > 12 months), and death. Of 1913 children and adolescents (48% female; median [IQR] age 11.5 [9.2-14.7] years at last clinic visit), 795 (42%) were disclosed to about their HIV status at a median age of 12.9 years (IQR: 11.8-14.1). During follow-up, 207 (11%) experienced disease progression, 75 (3.9%) were LTFU, and 59 (3.1%) died. There were lower hazards of disease progression (adjusted hazard ratio [aHR] 0.43 [0.28-0.66]) and death (aHR 0.36 [0.17-0.79]) for those disclosed to compared with those who were not. Disclosure and its appropriate implementation should be promoted in pediatric HIV clinics in resource-limited settings.
    MeSH terms: Adolescent; Asia/epidemiology; Child; Female; Humans; Male; Retrospective Studies; Disease Progression; Disclosure*; Lost to Follow-Up
  9. Tamjid Farki NNANL, Abdulhameed AS, Surip SN, ALOthman ZA, Jawad AH
    Int J Phytoremediation, 2023;25(12):1567-1578.
    PMID: 36794599 DOI: 10.1080/15226514.2023.2175780
    Herein, tropical fruit biomass wastes including durian seeds (DS) and rambutan peels (RP) were used as sustainable precursors for preparing activated carbon (DSRPAC) using microwave-induced H3PO4 activation. The textural and physicochemical characteristics of DSRPAC were investigated by N2 adsorption-desorption isotherms, X-ray diffraction, Fourier transform infrared, point of zero charge, and scanning electron microscope analyses. These findings reveal that the DSRPAC has a mean pore diameter of 3.79 nm and a specific surface area of 104.2 m2/g. DSRPAC was applied as a green adsorbent to extensively investigate the removal of an organic dye (methylene blue, MB) from aqueous solutions. The response surface methodology Box-Behnken design (RSM-BBD) was used to evaluate the vital adsorption characteristics, which included (A) DSRPAC dosage (0.02-0.12 g/L), (B) pH (4-10), and (C) time (10-70 min). The BBD model specified that the DSRPAC dosage (0.12 g/L), pH (10), and time (40 min) parameters caused the largest removal of MB (82.1%). The adsorption isotherm findings reveal that MB adsorption pursues the Freundlich model, whereas the kinetic data can be well described by the pseudo-first-order and pseudo-second-order models. DSRPAC exhibited good MB adsorption capability (118.5 mg/g). Several mechanisms control MB adsorption by the DSRPAC, including electrostatic forces, π-π stacking, and H-bonding. This work shows that DSRPAC derived from DS and RP could serve as a viable adsorbent for the treatment of industrial effluents containing organic dye.
    MeSH terms: Adsorption; Biodegradation, Environmental; Charcoal; Coloring Agents; Fruit/chemistry; Hydrogen-Ion Concentration; Kinetics; Methylene Blue/analysis; Microwaves; Seeds/chemistry; Bombacaceae*
  10. T Thurai Rathnam J, Grigg MJ, Dini S, William T, Sakam SS, Cooper DJ, et al.
    Malar J, 2023 Feb 14;22(1):54.
    PMID: 36782162 DOI: 10.1186/s12936-023-04483-9
    BACKGROUND: The incidence of zoonotic Plasmodium knowlesi infections in humans is rising in Southeast Asia, leading to clinical studies to monitor the efficacy of anti-malarial treatments for knowlesi malaria. One of the key outcomes of anti-malarial drug efficacy is parasite clearance. For Plasmodium falciparum, parasite clearance is typically estimated using a two-stage method, that involves estimating parasite clearance for individual patients followed by pooling of individual estimates to derive population estimates. An alternative approach is Bayesian hierarchical modelling which simultaneously analyses all parasite-time patient profiles to determine parasite clearance. This study compared these methods for estimating parasite clearance in P. knowlesi treatment efficacy studies, with typically fewer parasite measurements per patient due to high susceptibility to anti-malarials.

    METHODS: Using parasite clearance data from 714 patients with knowlesi malaria and enrolled in three trials, the Worldwide Antimalarial Resistance Network (WWARN) Parasite Clearance Estimator (PCE) standard two-stage approach and Bayesian hierarchical modelling were compared. Both methods estimate the parasite clearance rate from a model that incorporates a lag phase, slope, and tail phase for the parasitaemia profiles.

    RESULTS: The standard two-stage approach successfully estimated the parasite clearance rate for 678 patients, with 36 (5%) patients excluded due to an insufficient number of available parasitaemia measurements. The Bayesian hierarchical estimation method was applied to the parasitaemia data of all 714 patients. Overall, the Bayesian method estimated a faster population mean parasite clearance (0.36/h, 95% credible interval [0.18, 0.65]) compared to the standard two-stage method (0.26/h, 95% confidence interval [0.11, 0.46]), with better model fits (compared visually). Artemisinin-based combination therapy (ACT) is more effective in treating P. knowlesi than chloroquine, as confirmed by both methods, with a mean estimated parasite clearance half-life of 2.5 and 3.6 h, respectively using the standard two-stage method, and 1.8 and 2.9 h using the Bayesian method.

    CONCLUSION: For clinical studies of P. knowlesi with frequent parasite measurements, the standard two-stage approach (WWARN's PCE) is recommended as this method is straightforward to implement. For studies with fewer parasite measurements per patient, the Bayesian approach should be considered. Regardless of method used, ACT is more efficacious than chloroquine, confirming the findings of the original trials.

    MeSH terms: Animals; Bayes Theorem; Chloroquine/pharmacology; Humans; Parasites*; Plasmodium falciparum; Zoonoses; Plasmodium knowlesi*; Parasitemia/drug therapy; Parasitemia/parasitology
  11. Du Y, Shahiri H, Wei X
    SSM Popul Health, 2023 Mar;21:101347.
    PMID: 36785547 DOI: 10.1016/j.ssmph.2023.101347
    OBJECTIVES: The prevalence of unmet mental healthcare needs is a common challenge faced by many developing countries. This situation may worsen if more attention is not paid to the dramatic changes in the industrial workplace because of the diffusion of new automation and robotisation in the process of production.We aim to examine whether mental health problems are associated with frontline workers' direct experience of process innovation in the firms where they operate and verify whether/which of these mechanisms are involved in this relationship.

    METHODS: Our data were obtained from the Foshan Workplace Employee Survey (FWES). Mental health was proxied by the subjective assessment of workers' need to receive psychological counselling or treatment. To address endogeneity concerns, this study employed an extended ordered probit model and the two-stage least squares (2SLS) method.

    RESULTS: Frontline workers employed in innovative manufacturing firms are significantly more likely than those in firms taking no such action to experience psychological difficulties and to seek psychological counselling or treatment. Firms with a higher likelihood of upgrading their production process are more capable of taking a range of measures to significantly but not sufficiently mitigate the psychological problems of their workers induced by process innovation. In workplaces with a new advanced automation environment, workers believe that they face higher job insecurity (JI) and work stress, which in turn is partially and effectively linked to the deterioration in their mental health and further increases treatment-seeking behaviours.

    CONCLUSION: This study suggests that carrying out process innovation is associated with increased psychological distress and, hence, more needs for mental healthcare services. To narrow the treatment gap originally subject to existing obstacles, it is necessary to face the new challenges posed by automation-induced change in the workplace, which policies should be particularly attentive to.

  12. Mafarja M, Thaher T, Al-Betar MA, Too J, Awadallah MA, Abu Doush I, et al.
    Appl Intell (Dordr), 2023 Feb 09.
    PMID: 36785593 DOI: 10.1007/s10489-022-04427-x
    Software Fault Prediction (SFP) is an important process to detect the faulty components of the software to detect faulty classes or faulty modules early in the software development life cycle. In this paper, a machine learning framework is proposed for SFP. Initially, pre-processing and re-sampling techniques are applied to make the SFP datasets ready to be used by ML techniques. Thereafter seven classifiers are compared, namely K-Nearest Neighbors (KNN), Naive Bayes (NB), Linear Discriminant Analysis (LDA), Linear Regression (LR), Decision Tree (DT), Support Vector Machine (SVM), and Random Forest (RF). The RF classifier outperforms all other classifiers in terms of eliminating irrelevant/redundant features. The performance of RF is improved further using a dimensionality reduction method called binary whale optimization algorithm (BWOA) to eliminate the irrelevant/redundant features. Finally, the performance of BWOA is enhanced by hybridizing the exploration strategies of the grey wolf optimizer (GWO) and harris hawks optimization (HHO) algorithms. The proposed method is called SBEWOA. The SFP datasets utilized are selected from the PROMISE repository using sixteen datasets for software projects with different sizes and complexity. The comparative evaluation against nine well-established feature selection methods proves that the proposed SBEWOA is able to significantly produce competitively superior results for several instances of the evaluated dataset. The algorithms' performance is compared in terms of accuracy, the number of features, and fitness function. This is also proved by the 2-tailed P-values of the Wilcoxon signed ranks statistical test used. In conclusion, the proposed method is an efficient alternative ML method for SFP that can be used for similar problems in the software engineering domain.
  13. Tan JK, Zakaria SNA, Gunasekaran G, Abdul Sani NF, Nasaruddin ML, Jaafar F, et al.
    Oxid Med Cell Longev, 2023;2023:4416410.
    PMID: 36785791 DOI: 10.1155/2023/4416410
    Aging is a complex process characterized by progressive loss of functional abilities due to the accumulation of molecular damages. Metabolomics could offer novel insights into the predictors and mechanisms of aging. This cross-sectional study is aimed at identifying age-associated plasma metabolome in a Malay population. A total of 146 (90 females) healthy participants aged 28-69 were selected for the study. Untargeted metabolomics profiling was performed using liquid chromatography-tandem mass spectrometry. Association analysis was based on the general linear model. Gender-associated metabolites were adjusted for age, while age-associated metabolites were adjusted for gender or analyzed in a gender-stratified manner. Gender-associated metabolites such as 4-hydroxyphenyllactic acid, carnitine, cortisol, and testosterone sulfate showed higher levels in males than females. Deoxycholic acid and hippuric acid were among the metabolites with a positive association with age after being adjusted for gender, while 9(E),11(E)-conjugated linoleic acid, cortisol, and nicotinamide were negatively associated with age. In gender-stratified analysis, glutamine was one of the common metabolites that showed a direct association with age in both genders, while metabolites such as 11-deoxy prostaglandin F2β, guanosine monophosphate, and testosterone sulfate were inversely associated with age in males and females. This study reveals several age-associated metabolites in Malays that could reflect the changes in metabolisms during aging and may be used to discern the risk of geriatric syndromes and disorders later. Further studies are required to determine the interplay between these metabolites and environmental factors on the functional outcomes during aging.
    MeSH terms: Aged; Cross-Sectional Studies; Female; Humans; Hydrocortisone*; Malaysia; Male; Testosterone
  14. Lin Z, Jiang Y
    Heliyon, 2023 Feb;9(2):e13232.
    PMID: 36785821 DOI: 10.1016/j.heliyon.2023.e13232
    BACKGROUND: Career adaptability is an important building block for career success. Research on the career adaptability of disadvantaged students is therefore essential. The study aimed to explores the impact mechanism of career adaptability among impoverished students.

    METHODS: Using the Character Strengths Questionnaire, the Meaning in Life Questionnaire, the Personal Goal Scale and the Career Adaptability Scale, 1119 impoverished college students were surveyed.

    RESULTS: The results revealed that character strengths can not only positively predict career adaptability, but also indirectly affect career adaptability of impoverished students through the chain mediation meaning in life and personal goal. The results showed the mechanism of character strengths on the career adaptability among impoverished students.

    CONCLUSIONS: Our study found that meaning in life along with personal goal can enhance the positive impact of character strengths on career adaptability. These findings have implications for the practice of career counseling for impoverished college students.

  15. Garba K, Mohammed IY, Isa YM, Abubakar LG, Abakr YA, Hameed BH
    Heliyon, 2023 Feb;9(2):e13234.
    PMID: 36785823 DOI: 10.1016/j.heliyon.2023.e13234
    Canarium schweinfurthii fruit used in food and cosmetics produces waste nuts with a hard shell (hard-shell) and kernel. The hard-shell contained lignin and holocellulose, besides 51.99 wt% carbon, 6.0 wt% hydrogen, 41.68 wt% oxygen, and 70.97 wt% volatile matter. Therefore, this study commenced thermochemical investigations on the hard-shell through extensive intermediate pyrolysis and kinetic studies. During the active stage of thermogravimetric pyrolysis, the hard-shell lost a maximum of 56.45 wt%, and the activation energies obtained by the Kissinger-Akahira-Sunose, Flynn-Wall-Ozawa, and Starink methods were 223, 221 and 217 kJ/mol, respectively. The Flynn-Wall-Ozawa method depicted the degradation process accurately, where the Coat-Redfern method's contraction and diffusion mechanisms governed the pyrolysis reactions at activation energies of 16.62 kJ/mol and 38.83 kJ/mol, respectively. The pyrolysis process produced 25 wt% biochar and 25 wt% bio-oil under optimum conditions. The calorific values of the bio-oils with 6.81-7.11 wt% hydrogen and 68.01-71.12 wt% carbon was 26.32-27.83 MJ/kg, with phenolics and n-hexadecanoic and oleic acids as major compounds. Biochar, by contrast, has a high carbon content of 75.11-79.32 wt% and calorific values of 25.45-28.61 MJ/kg. These properties assert the biochar and bio-oils among viable bioenergy sources.
  16. Lim LL, Lau ESH, Kong APS, Fu AWC, Lau V, Jia W, et al.
    Lancet Reg Health West Pac, 2023 Mar;32:100663.
    PMID: 36785858 DOI: 10.1016/j.lanwpc.2022.100663
    BACKGROUND: In Asia, diabetes-associated death due to cardiorenal diseases were 2-3 times higher in women than men which might be due to gender disparity in quality of care and health habits.

    METHODS: Adults with type 2 diabetes (T2D) from 11 Asian countries/areas were assessed using the same protocol (2007-2015). We compared treatment target attainment (HbA1c < 7%, blood pressure [BP] < 130/80 mmHg, risk-based LDL-cholesterol, lack of central obesity [waist circumference <90 cm in men or <80 cm in women), use of cardiorenal-protective drugs (renin-angiotensin system [RAS] inhibitors, statins), and self-reported health habits including self-monitoring blood glucose (SMBG) by gender. Analyses were stratified by countries/areas, age of natural menopause (<50 vs. ≥50 years), and comorbidities (atherosclerotic cardiovascular disease [ASCVD], heart failure, kidney impairment [eGFR < 60 mL/min/1.73 m2]).

    FINDINGS: Among 106,376 patients (53.2% men; median (interquartile range) diabetes duration: 6.0 (2.0-12.0) years; mean ± SD HbA1c 8.0 ± 1.9%; 27% insulin-treated), women were older and less likely to receive college education than men (28.9% vs. 48.8%). Women were less likely to smoke/drink alcohol and were physically less active than men. Women had lower BP (<130/80 mmHg: 29.4% vs. 25.7%), less general obesity (54.8% vs. 57.8%) but more central obesity than men (77.5% vs. 57.3%). Women were less likely to have ASCVD (12.8% vs. 17.0%) or heart failure (1.3% vs. 2.3%), but more likely to have kidney impairment (22.3% vs. 17.6%) and any-site cancer than men (2.5% vs. 1.6%). In most countries/areas, more men attained HbA1c <7% and risk-based LDL-cholesterol level than women. After adjusting for potential confounders including countries and centres, men had 1.63 odds ratio (95% CI 1.51, 1.74) of attaining ≥3 treatment targets than women.

    INTERPRETATION: Asian women with T2D had worse quality of care than men especially in middle-income countries/areas, calling for targeted implementation programs to close these care gaps.

    SPONSOR: Asia Diabetes Foundation.

    FUNDING: Nil.

  17. Han C, Zheng Y, Wang L, Zhou C, Wang J, He J, et al.
    J Sci Food Agric, 2023 May;103(7):3334-3345.
    PMID: 36786016 DOI: 10.1002/jsfa.12499
    BACKGROUND: Extracted proteins of alternative animal origin tend to present strong off-flavor perception due to physicochemical interactions of coextracted off-flavor compounds with proteins. To investigate the relationship between absorption behaviors of volatile aromas and the processes-induced variations in protein microstructures and molecular conformations, duck liver protein isolate (DLp) was subjected to heating (65/100 °C, 15 min) and ultra-high pressure (UHP, 100-500 MPa/10 min, 28 °C) treatments to obtain differential unfolded protein states.

    RESULTS: Heat and UHP treatments induced the unfolding of DLp to varied degrees, as revealed by fluorescence spectroscopy, ultraviolet-visible absorption, circular dichroism spectra and surface hydrophobicity measurements. Two types of heating-denatured states with varied unfolding degrees were obtained, while UHP at both levels of 100/500 MPa caused partial unfolding of DLp and the presence of a molten-globule state, which significantly enhanced the binding affinity between DLp and (E,E)-2,4-heptadienal. In particular, significantly modified secondary structures of DLp were observed in heating-denatured samples. Excessive denaturing and unfolding degrees resulted in no significant changes in the absorption behavior of the volatile ligand, as characterized by observations of fluorescence quenching and analysis of headspace concentrations.

    CONCLUSION: Defining process-induced conformational transition behavior of matrix proteins could be a promising strategy to regulate food flavor attributes and, particularly, to produce DLp coextracted with limited off-flavor components by modifying their interaction during extraction processes. © 2023 Society of Chemical Industry.

    MeSH terms: Animals; Circular Dichroism; Ducks*; Liver; Protein Conformation; Protein Denaturation; Protein Folding*
  18. Govender HT, Krishnasamy G, Jani J, Husin LS
    Microbiol Resour Announc, 2023 Mar 16;12(3):e0100522.
    PMID: 36786591 DOI: 10.1128/mra.01005-22
    Streptomyces malaysiensis strain TY049-057 was isolated from a former tin-mining area in Bidor (Perak, Malaysia). Here, we report a draft genome sequence of S. malaysiensis strain TY049-057, with an estimated size of 2.7 Mb.
  19. Ismail H, Ahmad H, Sanef A, Shahabudin W, Reffin N, Chan D, et al.
    Am J Drug Alcohol Abuse, 2023 Jan 02;49(1):97-108.
    PMID: 36786756 DOI: 10.1080/00952990.2022.2161051
    Background: In recent years, amphetamine-type-stimulants (ATS) have been extensively misused in South-East Asia, inducing major problems among methadone-maintenance-treatment (MMT) patients.Objective: We examine ATS-misuse prevalence and its determinants among MMT patients in East-Coast-Malaysia.Methods: A retrospective-observational study was conducted on government-subsidized medication for opioid-use-disorder (MOUD) treatment clinics involving 292 MMT-patients (98% males) who were selected using a multilevel-proportional-stratified random sampling technique. Information obtained during the mandatory monitoring procedure from January 1 to December 31 2019, was utilized to determine ATS misuse prevalence from consecutive random urine drug screening. The determinants associated with its use, namely sociodemographics, social networks, comorbidities, and pharmacological assessments were analyzed via a logistic model.Results: Overall, 52.2% of the MMT-patients (95% CI: 0.42-0.54) had misused ATS at least once during their methadone treatment. These misusing patients were active smokers, hepatitis B and C seronegative, concurrently misused opioids, and had received suboptimum prescribed doses of methadone. Multiple logistic regression analysis demonstrated that the odds of misusing ATS during methadone treatment were 37 times higher among those who concurrently misused opioids (AOR: 37.60, 95% CI: 14.03-100.74) and 12 times higher among those who received suboptimal methadone doses (<60 mg/day) (AOR: 12.24, 95% CI: 5.58-25.43).Conclusion: This study demonstrated the significant prevalence of ATS misuse among MMT-patients in East-Coast-Malaysia, especially among patients who were prescribed suboptimal doses of methadone and/or demonstrated concomitant opioid-misuse. Stringent urine-monitoring is crucial to prevent diversion to ATS misuse. Nonetheless, to avoid negligence and improve physician engagement, the relevant authorities should immediately plan a comprehensive national-standard training module comprising support activities for professional methadone-prescribers.
    MeSH terms: Amphetamine/therapeutic use; Analgesics, Opioid/therapeutic use; Female; Humans; Malaysia; Male; Methadone/therapeutic use; Retrospective Studies; Opiate Substitution Treatment/methods
  20. Zambry NS, Awang MS, Beh KK, Hamzah HH, Bustami Y, Obande GA, et al.
    Lab Chip, 2023 Mar 14;23(6):1622-1636.
    PMID: 36786757 DOI: 10.1039/d2lc01159j
    The emergence of coronavirus disease 2019 (COVID-19) motivates continuous efforts to develop robust and accurate diagnostic tests to detect severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Detection of viral nucleic acids provides the highest sensitivity and selectivity for diagnosing early and asymptomatic infection because the human immune system may not be active at this stage. Therefore, this work aims to develop a label-free electrochemical DNA biosensor for SARS-CoV-2 detection using a printed circuit board-based gold substrate (PCBGE). The developed sensor used the nucleocapsid phosphoprotein (N) gene as a biomarker. The DNA sensor-based PCBGE was fabricated by self-assembling a thiolated single-stranded DNA (ssDNA) probe onto an Au surface, which performed as the working electrode (WE). The Au surface was then treated with 6-mercapto-1-hexanol (MCH) before detecting the target N gene to produce a well-oriented arrangement of the immobilized ssDNA chains. The successful fabrication of the biosensor was characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and atomic force microscopy (AFM). The DNA biosensor performances were evaluated using a synthetic SARS-CoV-2 genome and 20 clinical RNA samples from healthy and infected individuals through EIS. The developed DNA biosensor can detect as low as 1 copy per μL of the N gene within 5 minutes with a LOD of 0.50 μM. Interestingly, the proposed DNA sensor could distinguish the expression of SARS-CoV-2 RNA in a patient diagnosed with COVID-19 without any amplification technique. We believe that the proposed DNA sensor platform is a promising point-of-care (POC) device for COVID-19 viral infection since it offers a rapid detection time with a simple design and workflow detection system, as well as an affordable diagnostic assay.
    MeSH terms: DNA/chemistry; DNA, Single-Stranded; Electrodes; Gold/chemistry; Humans; RNA, Viral; Biosensing Techniques*; Electrochemical Techniques
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