Browse publications by year: 2024

  1. Wen CWQ, Saei NM, Ahanin E, Teixeira FB, Parolia A
    Aust Endod J, 2024 Dec;50(3):525-537.
    PMID: 38853582 DOI: 10.1111/aej.12863
    This study evaluated the technical quality (TQ) of root canal therapy (RCT) performed by predoctoral students and its impact on endodontic outcome (EO) and patients' quality of life (QoL). The TQ of RCT done by predoctoral students was evaluated and follow-up visits were conducted to determine the clinical, radiographic outcome of RCT and patients' QoL. Frequency distribution, multiple regression, independent-samples t test and one-way anova were performed. A total of 226 teeth of 164 patients were clinically and radiographically examined. A satisfactory TQ was observed in 130 (57.5%), successful clinical outcomes in 155 (68.6%), successful radiographical outcomes in 206 (91%) and overall successful EO in 150 teeth (66.4%) with 80% of patients reporting a favourable QoL. A significant positive correlation was noted between EO and QoL (p = 0.002) with no significant correlation in between TQ-RCT and EO (p = 0.07) and TQ-RCT and QoL (p = 0.316). Successful EO had a positive impact on patients' QoL.
    MeSH terms: Adolescent; Adult; Aged; Clinical Competence; Education, Dental/methods; Education, Dental/standards; Endodontics/education; Female; Humans; Male; Middle Aged; Quality of Life*; Treatment Outcome; Young Adult
  2. Lee EYN, Sua ZY, Bhatia S, Kohli S, Rossi-Fedele G, Doğramacı EJ, et al.
    Aust Endod J, 2024 Dec;50(3):559-570.
    PMID: 38924249 DOI: 10.1111/aej.12868
    This retrospective cohort study compared the effect of primary root canal treatment (RCT) with root canal retreatment (Re-RCT) on patient-reported outcomes in Kuala Lumpur, Malaysia. Forty randomly selected adults participated (RCT n = 20; Re-RCT n = 20). The impact their dentition had on the Oral Health Impact Profile-14 (OHIP-14) was assessed by calculating the prevalence of oral health impact, and the severity score. Focus group discussions using a semi-structured guide were arranged through an online meeting platform. Qualitative content analysis identified common themes, and relevant quotes gathered. The impact on OHIP-14 was limited for both RCT and Re-RCT groups with no significant differences in the prevalence of oral health impact. Significant differences were found for functional limitation (RCT higher) and psychological discomfort (Re-RCT higher). Common themes from the discussions include the importance of retaining teeth, the significance of effective communication between clinicians and patients and that the respondents were satisfied with the treatment.
    MeSH terms: Adult; Female; Humans; Malaysia; Male; Middle Aged; Oral Health*; Quality of Life*; Retrospective Studies; Patient Satisfaction; Retreatment*; Patient Reported Outcome Measures
  3. Mohd Rozi NA, Mohd Tahir NA, Mohd Saffian S, Makmor-Bakry M, Mohamad Yusof A, Mustafar R, et al.
    J Antimicrob Chemother, 2024 Dec 02;79(12):3078-3090.
    PMID: 39321326 DOI: 10.1093/jac/dkae332
    BACKGROUND: Continuous renal replacement therapy (CRRT) complicates antibiotic dosing in critically ill patients due to altered pharmacokinetics. The optimal dosing of piperacillin remains unclear. Therapeutic drug monitoring (TDM) can personalize piperacillin therapy and improve outcomes.

    OBJECTIVES: This review investigates the effects of TDM-guided piperacillin dosing on pharmacokinetic target attainment and clinical outcomes in CRRT patients, analyses correlations with clinical outcomes, provides optimal dosing strategies for piperacillin and identifies future research areas.

    METHODS: A systematic search of PubMed, Scopus and Web of Science was conducted until December 2023, identifying studies on piperacillin pharmacokinetics and clinical outcomes in adult CRRT patients. Data on study characteristics, piperacillin exposures, TDM use, target attainment rates, mortality and length of stay were extracted. The risk of bias was assessed using the Newcastle-Ottawa Scale.

    RESULTS: Eleven observational studies were included. High pharmacokinetic variability was evident, with piperacillin target non-attainment in up to 74% of cases without TDM. Two studies with routine TDM showed increased target attainment rates of 80%-100%. Mortality ranged from 17% to 56%, with supratherapeutic concentrations (≥100 mg/L) associated with higher mortality. The impact of optimized piperacillin exposures on outcomes was inconclusive. Most studies demonstrated a low risk of bias.

    CONCLUSIONS: TDM-guided piperacillin dosing in CRRT patients improved target attainment rates (≥80%). Mortality rates ranged from 17% to 56%, with inconsistent correlations between drug exposures and survival. Supratherapeutic concentrations were linked to higher mortality. Standardized TDM protocols are needed. Future research should establish clear exposure-response relationships and the impact of TDM on clinical outcomes.

    MeSH terms: Humans; Critical Illness*; Drug Monitoring*; Observational Studies as Topic
  4. Chong ZX
    Biochim Biophys Acta Rev Cancer, 2024 Nov;1879(6):189191.
    PMID: 39353485 DOI: 10.1016/j.bbcan.2024.189191
    Ovarian cancer is one of the gynaecology malignancies with the highest mortality rate. Ovarian cancer stem cell (CSC) is a subpopulation of ovarian cancer cells with increased self-renewability, aggression, metastatic potentials, and resistance to conventional anti-cancer therapy. The emergence of ovarian CSC is a critical factor that promotes treatment resistance and frequent relapse among ovarian cancer patients, leading to poor clinical outcomes. MicroRNA (miRNA) is a short, non-protein-coding RNA that regulates ovarian CSC development. Although multiple original research articles have discussed the CSC-regulatory roles of different miRNAs in ovarian cancer, there is a deficiency of a review article that can summarize the findings from different research papers. To narrow the gap in the literature, this review aimed to provide an up-to-date summary of the CSC-regulatory roles of various miRNAs in modulating ovarian cancer cell stemness. This review will begin by giving an overview of ovarian CSC and the pathways responsible for driving its appearance. Next, the CSC-regulatory roles of miRNAs in controlling ovarian CSC development will be discussed. Overall, more than 60 miRNAs have been reported to play CSC-regulatory roles in the development and progression of ovarian cancer. By targeting various downstream targets, these miRNAs can control the signaling activities of PI3K/AKT, EGFR/ERK, WNT/ß-catenin, NF-kß, Notch, Hippo/YAP, EMT, and DNA repair pathways. Hence, these CSC-modulatory miRNAs have the potential to be used as prognostic biomarkers in predicting the clinical outcomes of ovarian cancer patients. Targeting CSC-promoting miRNAs or increasing the expressions of CSC-repressing miRNAs can help slow ovarian cancer progression. However, more in-depth functional and clinical trials must be carried out to evaluate the suitability, safety, sensitivity, and specificity of these CSC-regulating miRNAs as prognostic biomarkers or therapeutic targets.
    MeSH terms: Animals; Female; Humans; Biomarkers, Tumor/genetics; Biomarkers, Tumor/metabolism; Signal Transduction; Gene Expression Regulation, Neoplastic*
  5. Lee JL, Mohamed Shah N, Makmor-Bakry M, Islahudin F, Alias H, Mohd Saffian S
    Clin Ther, 2024 Dec;46(12):e25-e37.
    PMID: 39366801 DOI: 10.1016/j.clinthera.2024.09.018
    PURPOSE: Intravenous immunoglobulin (IVIG) is used to treat various immune system disorders, but the factors influencing its disposition are not well understood. This study aimed to estimate the population pharmacokinetic parameters of IVIG and to investigate the effect of genetic polymorphism of the FCGRT gene encoding the neonatal Fc receptor (FcRn) and clinical variability on the pharmacokinetic properties of IVIG in patients with immune system disorders.

    METHODS: Patients were recruited from 4 hospitals in Malaysia. Clinical data were recorded, and blood samples were taken for pharmacokinetic and genetic studies. Population pharmacokinetic parameters were estimated by nonlinear mixed-effects modeling in Monolix. Age, weight, baseline immunoglobulin G concentration, ethnicity, sex, genotype, disease type, and comorbidity were investigated as potential covariates. Models were evaluated using the difference in objective function value, goodness-of-fit plots, visual predictive checks, and bootstrap analysis.

    FINDINGS: A total of 292 blood samples were analyzed from 79 patients. The IVIG concentrations were best described by a 2-compartment model with linear elimination. Weight was found to be an important covariate for volume of distribution in the central compartment (Vc), volume of distribution in the peripheral compartment (Vp), and clearance in the central compartment, whereas disease type was found to be an important covariate for Vp. Goodness-of-fit plots indicated that the model fit the data adequately. Genetic polymorphism of the FCGRT gene encoding the neonatal Fc receptor did not affect the pharmacokinetic properties of IVIG.

    IMPLICATIONS: This study supports the use of dosage based on weight as per current practice. The study findings highlight that Vp is significantly influenced by the type of disease being treated with IVIG. This relationship suggests that different disease types, particularly inflammatory and autoimmune conditions, may alter tissue permeability and fluid distribution due to varying degrees of inflammation. Increased inflammation can lead to enhanced permeability and retention of IVIG in peripheral tissues, reflecting higher Vp values.

    MeSH terms: Adolescent; Adult; Aged; Child; Female; Genotype; Humans; Malaysia; Male; Middle Aged; Models, Biological; Polymorphism, Genetic; Histocompatibility Antigens Class I/genetics; Young Adult
  6. Chakraborty S, Vishwas S, Harish V, Gupta G, Paudel KR, Dhanasekaran M, et al.
    Expert Opin Drug Deliv, 2024 Dec;21(12):1771-1792.
    PMID: 39397403 DOI: 10.1080/17425247.2024.2414768
    INTRODUCTION: Alzheimer's disease (AD) stands as significant challenge in realm of neurodegenerative disorder. It is characterized by gradual decline in cognitive function and memory loss. It has already expanded its prevalence to 55 million people worldwide and is expected to rise significantly. Unfortunately, there exists a limited therapeutic option that would mitigate its progression. Repurposing existing drugs and employing nanoparticle as delivery agent presents a potential solution to address the intricate pathology of AD.

    AREAS COVERED: In this review, we delve into utilization of nanoparticular platforms to enhance the delivery of repurposed drugs for treatment of AD. Firstly, the review begins with the elucidation of intricate pathology underpinning AD, subsequently followed by rationale behind drug repurposing in AD. Covered are explorations of nanoparticle-based repurposing of drugs in AD, highlighting their clinical implication. Further, the associated challenges and probable future perspective are delineated.

    EXPERT OPINION: The article has highlighted that extensive research has been carried out on the delivery of repurposed nanomedicines against AD. However, there is a need for advanced and long-term research including clinical trials required to shed light upon their safety and toxicity profile. Furthermore, their scalability in pharmaceutical set-up should also be validated.

    MeSH terms: Animals; Humans; Drug Delivery Systems*; Disease Progression; Nanoparticles*; Drug Repositioning*
  7. Tripathi T, Mohan S, Alfaifi HA, Farasani A, R R, Sharma P, et al.
    Int J Surg, 2024 Dec 01;110(12):7573-7584.
    PMID: 39468970 DOI: 10.1097/JS9.0000000000002125
    BACKGROUND: Fistulas, abnormal connections between two anatomical structures, significantly impact the quality of life and can result from a variety of causes, including congenital defects, inflammatory conditions, and surgical complications. Stem cell therapy has emerged as a promising alternative due to its potential for regenerative and immunomodulatory effects. This overview of systematic reviews aimed to assess the safety and efficacy of stem cell therapy in managing fistulas, drawing on the evidence available.

    METHODS: This umbrella review was conducted following the Joanna Briggs Institute (JBI) methodology to assess the efficacy and safety of stem cell therapy for treating various types of fistulas. A comprehensive search was performed across multiple electronic databases including PubMed, Embase, Cochrane Register, and Web of Science up to 5 May 2024. Systematic reviews focusing on stem cell therapy for fistulas were included, with data extracted on study design, stem cell types, administration methods, and outcomes. The quality of the reviews was assessed using the AMSTAR 2 tool, and meta-analyses were conducted using R software version 4.3.

    RESULTS: Nineteen systematic reviews were included in our umbrella review. The stem cell therapy demonstrated by significant improvements in clinical remission rates, with a relative risk (RR) of 1.299 (95% CI: 1.192-1.420). Stem cell therapy enhanced fistula closure rates, both short-term (RR=1.481; 95% CI: 1.036-2.116) and long-term (RR=1.422; 95% CI: 1.091-1.854). The safety analysis revealed no significant increase in the risk of adverse events with stem cell therapy, showing a pooled RR of 0.972 (95% CI: 0.739-1.278) for general adverse events and 1.136 (95% CI: 0.821-1.572) for serious adverse events, both of which indicate a safety profile comparable to control treatments. Re-epithelialization rates also improved (RR=1.44; 95% CI: 1.322-1.572).

    CONCLUSION: Stem cell therapy shows promise as an effective and safe treatment for fistulas, particularly in inducing remission and promoting closure of complex fistulas. The findings advocate for further high-quality research to confirm these benefits and potentially incorporate stem cell therapy into standard clinical practice for fistula management. Future studies should focus on long-term outcomes and refining stem cell treatment protocols to optimize therapeutic efficacy.

    MeSH terms: Fistula/therapy; Humans; Quality of Life; Treatment Outcome
  8. Sarker S, Talukder S, Bhuiyan MSA, Haque MH
    Microbiol Resour Announc, 2024 Dec 12;13(12):e0082024.
    PMID: 39470235 DOI: 10.1128/mra.00820-24
    This study reveals the genomes of psittaciform chaphamaparvovirus 5 (PsChPV-5) and a beak and feather disease virus (BFDV), discovered in the fecal samples of cockatiels. The genomes of PsChPV-5 and BFDV are 4,366 and 2,009 base pairs long, respectively, each exhibiting the characteristic genomic structures of their respective genera.
  9. Abdul Malek AZ, Hashim AM, Khairil Mokhtar NF, Saidi NB, Abu Bakar MF, Singaram N
    Microbiol Resour Announc, 2024 Dec 12;13(12):e0054224.
    PMID: 39470238 DOI: 10.1128/mra.00542-24
    This study examines the genome sequences of five endophytic bacterial isolates from the Oryza sativa microbiome to assess their potential as plant bio-inoculants. The five complete bacterial genomes from the genera Pseudomonas, Burkholderia, Sphingobacterium, Stenotrophomonas, and Pantoea were sequenced using Nanopore long-read sequencing technology.
  10. Naing C, Ni H, Basavaraj AK, Aung HH, Tung WS, Whittaker MA
    Acta Trop, 2024 Dec;260:107447.
    PMID: 39477046 DOI: 10.1016/j.actatropica.2024.107447
    This study aimed to synthesise evidence comparing the levels of cytokines in severe falciparum malaria with those in uncomplicated malaria from available systematic reviews and meta- analyses. Relevant individual meta-analyses were searched in PubMed, Ovid, and Google Scholar, following the selection criteria specified for this umbrella review. The AMSTAR-2 tool was applied to grade the quality of the meta-analyses identified. The random-effects model was applied to recalculate the effect sizes of each included meta-analysis. Heterogeneity between meta-analyses was investigated with I2 value. 95% predicting interval (PI) for the summary random-effects model was also made. In each meta-analysis identified, information on largest study's effect, the excess significance test, small study effects, and publication bias were addressed. This umbrella review included nine meta-analyses (n = 12,674) for nine unique cytokines (IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, and TNF-α). Only one individual meta-analysis showed significantly higher levels of cytokine IL-1β (p: 0.009) amongst those with severe falciparum malaria compared to those with uncomplicated malaria. The 95% PIs did not show significance in any individual meta-analyses. Nine individual meta-analyses showed substantial heterogeneity, with I2 tests ranging from 81% to 99%. Two independent meta-analyses (the IL-4 and IL-12) showed evidence of 'excess significant bias'. The meta-analysis of IL-1β only showed "Class III evidence", indicating that this cytokine was "suggestive" in contributing to those with severity of malaria in comparison to those with uncomplicated malaria. The remaining eight cytokines showed "Class IV evidence," indicating "weak" evidence on the impact of malaria severity. In conclusion, the findings suggest that compared to uncomplicated malaria, pro-inflammatory cytokine IL-1β contributes to the development of severe falciparum malaria. Due to the limited level of evidence, further well-designed larger studies with multiple cytokines are needed to investigate cytokine levels as reliable biomarkers in malaria severity.
    MeSH terms: Humans; Plasmodium falciparum/immunology; Severity of Illness Index
  11. Smith P, Joseph A, Baker-Austin C, Kang N, Baron S, Le Devendec L, et al.
    Dis Aquat Organ, 2024 Dec 12;160:127-134.
    PMID: 39665310 DOI: 10.3354/dao03831
    This work was performed to generate the data needed to set epidemiological cut-off values for minimal inhibitory concentrations (MICs) of 10 antimicrobial agents against Vibrio parahaemolyticus determined using standardised broth microdilution protocols. Eight laboratories performed broth microdilution tests with incubation at 35°C for 16 to 20 h, and 7 also performed tests on the same isolates with incubation at 28°C for 24 to 28 h. Data were analysed by the ECOFFinder and normalised resistance interpretation algorithms. The cut-off values calculated for ceftazidime, florfenicol and trimethoprim/sulfamethoxazole, 1, 1 and 0.25/4.75 µg ml-1, respectively, were the same when calculated from data obtained at both temperatures. The cut-off values calculated from data obtained at 35°C and from data obtained at 28°C were 0.25 and 0.5 µg ml-1 for enrofloxacin, 2 and 4 µg ml-1 for gentamicin, 0.5 and 1 µg ml-1 for oxolinic acid and 2 and 1 µg ml-1 for oxytetracycline, respectively. The influence of incubation temperature on MIC values was investigated by comparing MICs obtained at 35 and 28°C for a specific antimicrobial agent with a particular isolate by an individual laboratory. Results showed that 56% of 1473 of these paired MIC values were identical, while 38% differed from one another by not more than 1 dilution step. The data generated in this work will be submitted to the Clinical and Laboratory Standards Institute for consideration in their setting of internationally agreed epidemiological cut-off values for V. parahaemolyticus that are essential for interpreting antimicrobial susceptibility testing data of this species.
    MeSH terms: Animals; Microbial Sensitivity Tests*; Temperature*; Drug Resistance, Bacterial
  12. Irfan AMSM, Geoffrey S, Htet H, Krishnappa P, Razali N, Iezhitsa I, et al.
    J Ocul Pharmacol Ther, 2024 Dec 13.
    PMID: 39665694 DOI: 10.1089/jop.2024.0131
    Purpose: Renin-angiotensin system (RAS) is expressed in neuronal tissue and plays a role in neurodegenerative diseases involving excitotoxicity as a pathophysiological mechanism. In retina, excessive excitatory neurotransmission via N-methyl-d-aspartate (NMDA) receptors underlies neuronal apoptosis in conditions like glaucoma. However, it is not known if NMDA-mediated excitotoxicity alters retinal RAS expression. Hence, this study investigated the effect of NMDA exposure on the expression of RAS in rat retinas. Methods: Two groups of Sprague-Dawley rats received either phosphate buffer saline or NMDA (160 nmol). On day 7 posttreatment, retinal expression of RAS components including renin, angiotensinogen, angiotensin-converting enzyme (ACE), angiotensin II (Ang II), Ang 1-7, Ang 1-9, MAS receptor, angiotensin II type 1 receptor (AT1R), ACE2, and aldosterone was measured using enzyme-linked immunosorbent assay and polymerase chain reaction. Morphometric studies were done to assess morphological alterations. Results: Following the exposure to NMDA, an upregulation of ACE expression was noted at both the protein (2.03-folds; P < 0.001) and mRNA (1.86-folds; P < 0.01) levels in rat retinas. AT1R protein and mRNA expression were greater by 1.73 (P < 0.0001) and 2.28-folds (P < 0.0001), respectively. However, mRNA expression for ACE2, Ang 1-7, and Ang 1-9, showed a 1.51-(P < 0.05), 2.41-(P < 0.001), and 2.37-(P < 0.0001) fold decrease. Ganglion cell layer (GCL) thickness and linear cell density in GCL were significantly lower in the NMDA-treated group (P < 0.05). Conclusions: NMDA exposure increases expression of the classical RAS and suppresses that of alternate RAS in rat retinas. These alterations are associated with retinal morphological changes indicating significant loss of neuronal cells in the GCL of rat retinas.
  13. Lou KJQ, Rajaram NN, Say YH
    PLoS One, 2024;19(12):e0309507.
    PMID: 39666616 DOI: 10.1371/journal.pone.0309507
    This study investigated the availability, price, nutritional composition, and quality of plant-based meat alternatives (PBMAs) in supermarkets and hypermarkets in Petaling, the most populated district in Malaysia. The survey covered 85 stores through on-site visits, identifying 251 unique PBMA products from 42 brands. The PBMAs were categorized into nine groups: Burgers/Patties, Coated Meat, Luncheon Meat, Minced Meat, Pastries, Pieces/Chunks/Fillets/Strips, Prepacked Cooked Meals, Sausages, and Seafood Balls/Cakes/Meatballs. The survey indicated widespread availability of PBMAs in Petaling, with premium supermarkets offering the most extensive selection. The median prices of PBMAs across categories were below MYR 7 (~USD 1.6) per 100 grams, with Pieces/Chunks/Fillets/Strips being the most affordable. Nutritionally, PBMAs exhibited lower energy, total fat, saturated fat, sodium, and protein levels than meat equivalents, while showing higher carbohydrate content. Nutri-Score profiling revealed generally favourable scores (Grade A to C), indicating moderate to good nutritional quality. These findings suggest that PBMAs offer a viable alternative for consumers in Malaysia seeking healthier and more sustainable food options, particularly for those aiming to reduce their intake of fat and sodium. However, PBMAs may not be recommended for individuals seeking a higher protein and lower carbohydrate diet. Further research is recommended to explore micronutrient profiles to enhance dietary decision-making.
    MeSH terms: Animals; Commerce; Humans; Malaysia; Meat/economics; Meat Products/analysis; Meat Products/economics; Nutritive Value*; Nutrition Assessment
  14. Wang X, Maidin SS, Batumalay M
    PLoS One, 2024;19(12):e0315424.
    PMID: 39666652 DOI: 10.1371/journal.pone.0315424
    With the development of integrated circuit packaging technology, the layout of printed circuit boards has become complicated. Moreover, the traditional defect detection methods have been difficult to meet the requirements of high precision. Therefore, in order to solve the problem of low efficiency in defect detection of printed circuit boards, a defect detection method based on pseudo-inverse transform and improved YOLOv5 is proposed. Firstly, a defect image restoration model is constructed to improve image clarity. Secondly, Transformer is introduced to improve YOLOv5, and the batch normalization and network loss function are optimized. These methods improve the speed and accuracy of PCB defect detection. Experimental verification showed that the restoration speed of the image restoration model was 37.60%-42.38% higher than other methods. Compared with other models, the proposed PCB defect detection model had an average increase of 10.90% in recall and 12.87% in average detection accuracy. The average detection accuracy of six types of defects in the self-made PCB data set was over 98.52%, and the average detection accuracy was as high as 99.1%. The results demonstrate that the proposed method can enhance the quality of image processing and optimize YOLOv5 to improve the accuracy of detecting defects in printed circuit boards. This method is demonstrably more effective than existing technology, offering significant value and potential for application in industrial contexts. Its promotion could facilitate the advancement of industrial automation manufacturing.
    MeSH terms: Algorithms; Electronics; Models, Theoretical
  15. Al-Battawi S, Latif MT, How V, Thilakavathy K, Hamid HHA, Tan CK, et al.
    PLoS One, 2024;19(12):e0315439.
    PMID: 39666673 DOI: 10.1371/journal.pone.0315439
    Motor vehicles emit most Malaysian PAHs in particulate matter of 2.5 μm (PM2.5-bound PAHs). Although traffic-related air pollution harms healthy people, there is a knowledge gap regarding PAHs' effects on Malaysians. This study examines PM2.5-bound PAH concentrations, distribution, sources, and health risks in Malaysia's high and low-traffic zones. Kuala Lumpur (KL) and Hulu Langat (HL) exhibit Malaysia's high- and low-traffic areas. The high-volume air sampler collected 40 ambient PM2.5 samples at both locations. Solid-phase extraction and gas chromatography-mass spectrometry (GC-MS) assessed PAHs. The mean PM2.5-bound PAH concentrations in KL (5.85 ng m-3) were significantly higher than in HL (0.55 ng m-3) (p<0.001). KL has nine times more low-molecular-weight PAHs (LMW-PAHs) (2.63 vs. 0.27 ng m-3) and eleven times more high-molecular-weight PAHs (HMW-PAHs) (3.22 vs. 0.28 ng m-3) than HL. Over 51% of PM2.5 air samples at both sites included HMW-PAHs. Source apportionment tools (Diagnostic ratio, positive matrix factorization, and principal component analysis) showed that fossil fuel combustions (petrol and diesel) produced the greatest PAHs in both locations. Moreover, PAH exposure impinged higher carcinogenic health risks in KL than in HL. In conclusion, traffic and automobile pollution account for the short- and long-term health risks posed by PAHs in both regions.
    MeSH terms: Air Pollutants/analysis; Air Pollution/analysis; Environmental Monitoring/methods; Humans; Malaysia; Rural Population
  16. Guo Q, Jamil H, Ismail L, Luo S, Sun Z
    PLoS One, 2024;19(12):e0307819.
    PMID: 39666681 DOI: 10.1371/journal.pone.0307819
    Teaching English as a foreign language (EFL) is a priority globally, but pedagogical methods do not always keep up with the evolving needs of learners. Problem-based learning (PBL) is an innovative pedagogical approach that facilitates students' self-regulated learning, thereby improving their English proficiency. The present systematic literature review therefore concentrates on the application of PBL methodology in improving students' English language proficiency. It was conducted according to the systematic review and meta-analysis Preferred Reporting Items for Meta-Analyses (PRISMA) review methodology. In total, 27 articles related to PBL to improve English proficiency published between 2012 and 2023 were identified from Web of Science, Scopus, ProQuest, ERIC, and ScienceDirect databases. In the light of the findings, PBL has a positive effect on students' behaviour, academic performance, and critical thinking. Consequently, this paper contributes to policy makers, educators, and students to improve the English proficiency of students at all levels of education using PBL approach.
    MeSH terms: Humans; Language; Learning; Students; Multilingualism
  17. Hariyanto AP, Mugni FF, Khumaira L, Sensusiati AD, Nursela AL, Suprijanto, et al.
    Radiography (Lond), 2024 Dec 11;31(1):254-263.
    PMID: 39667263 DOI: 10.1016/j.radi.2024.11.023
    INTRODUCTION: Phantom materials with tissue-equivalent physical properties that require regular evaluation using patented phantoms are essential for medical device quality assurance programs. This study evaluated phantom materials for tissue equivalence and their use in image quality assessment for breast ultrasound scanner performance testing using two custom-made phantoms.

    METHODS: Two types of phantoms were developed: phantoms A and B. Phantom A was made from a base material consisting of polyvinyl chloride-plastisol with the addition of glycerol, whereas phantom B consisted of polyvinyl chloride-plastisol with the addition of graphite. Each phantom had a stiff and soft lesion shaped like a sphere, with a diameter of 1.4 cm. The phantoms were cuboids with dimensions of 10 × 10 cm2 and a thickness of 5 cm. A series of phantom evaluations was performed, consisting of density, elasticity, acoustic properties, B-mode ultrasound images, and strain ratio.

    RESULTS: The characterisation results show that background A closely resembles fibroglandular tissue in terms of density and acoustic properties (<5% variation); background B only resembles fibroglandular tissue in terms of density (-1.8% variation). In terms of elasticity, both backgrounds were close to the minimum value of fibroglandular tissue elasticity. The soft lesion on the phantom had a slightly lower density and elasticity than the carcinoma, whereas its acoustic properties (speed of sound and attenuation coefficient) were slightly higher than those of the reference carcinoma. Both phantoms were consistent with the literature in terms of strain ratio, geometric accuracy, lesion detection, and mean pixel value and showed good potential stability over one year.

    CONCLUSION: This study successfully described the fabrication and evaluation sequence of a phantom equivalent to breast fibroglandular tissue and its evaluation via ultrasound imaging.

    IMPLICATIONS FOR PRACTICE: This study offers proprietary information essential for the fabrication of phantoms that can be used for quality assurance and control in ultrasound imaging.

  18. Schwartz MK, Dunn SL, Gendron WAC, Helm JE, Kamau WS, Mark-Shadbolt M, et al.
    Trends Ecol Evol, 2024 Dec 11.
    PMID: 39667987 DOI: 10.1016/j.tree.2024.11.011
    Introducing new genes and new species into ecosystems where they have not previously existed presents opportunities and complex, multivalue decisions for conservation biologists and the public. Both synthetic biology and conservation introductions offer potential benefits, such as avoiding extinctions and restoring ecological function, but also carry risks of unintended ecological consequences and raise social and moral concerns. Although the conservation community has attempted to establish guidelines for each new tool, there is a need for comprehensive principles that will enable conservation managers to navigate emerging technologies. Here, we combine biological, legal, social, cultural, and ethical considerations into an inclusive set of principles designed to facilitate the efforts of managers facing high-consequence conservation decisions by clarifying the stakes of inaction and action, along with the use of decision frameworks to integrate multiple considerations.
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