Browse publications by year: 2025

  1. King TL, Tan SH, Tan SSN, Lai WH, Bujang MA, Voon PJ
    Sci Rep, 2025 Jan 10;15(1):1626.
    PMID: 39794348 DOI: 10.1038/s41598-024-83626-7
    Despite the expanding landscape of clinical trials, there is a lack of study concerning Malaysian patients' participation and perspectives. This study addresses these gaps by assessing patients' willingness, knowledge, perceptions, confidence, and religious barriers related to clinical trial participations at Sarawak General Hospital. We conducted a cross-sectional survey from March to September 2022 on 763 cancer and non-cancer patients. We collected patients' responses and calculated scores for willingness to participate (40.5/100), knowledge (29.9/100), perceived benefits (66.5/100) and risks (72.4/100) of participations, confidence in clinical trial conducts (66.3/100), and religious barriers (49.8/100). The higher scores indicated greater willingness, better knowledge, stronger perceptions of benefits and risk, increased confidence, and stronger religious barriers. Cancer patient demonstrated significantly greater willingness for trials involving new drugs (31.9/100 vs. 27.4/100, p = 0.021) but slightly higher religious barriers compared to non-cancer cohort (51.4/100 vs. 48.3/100, p = 0.006). Multivariable logistic regression identified female gender, unemployment, poor knowledge, low perceived benefits, high perceived risks, and low confidence as significant factors associated with reduced willingness to participate (p 
    MeSH terms: Adult; Aged; Clinical Trials as Topic*; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Middle Aged; Surveys and Questionnaires
  2. Destiani DP, Dewi S, Sulaiman SAS, Alfian SD, Barliana MI, Abdulah R
    Sci Rep, 2025 Jan 10;15(1):1647.
    PMID: 39794365 DOI: 10.1038/s41598-024-69604-z
    Rheumatoid Arthritis (RA) is a chronic and progressive autoimmune disease that affects synovial tissues has greater risk of developing secondary osteoporosis (OP). In particular, polymorphisms in Adenosine Monophosphate Deaminase 1 (AMPD1) and Methylenetetrahydrofolate Reductase (MTHFR) affect the outcome of methotrexate (MTX) treatment in patients with RA. Therefore, this study aimed to determine the association of AMPD1 rs17602729, MTHFR C677T, and MTHFR A1298C polymorphisms with MTX activity in RA patients. A retrospective design was adopted to collect data from medical records and blood samples of 99 patients experiencing outpatient care at a referral hospital in Bandung. The inclusion criteria were patients diagnosed with RA, aged 18-59 years, and receiving MTX therapy for ≥ 6 months. DNA was isolated and then amplified using Polymerase Chain Reaction (PCR), and genotyping was performed with Sanger sequencing. The kinetic photometric method was used to measure the levels of calcium in the samples. The results showed that there is no significant association between the MTHFR C677T genotype variant or allele with calcium levels, as indicated by p-values of 0.177 and 0.174, respectively. The association between the MTHFR A1298C genotype variant or alleles with calcium levels was also not significant (p = 0.206 and p = 0.090, respectively). However, most patients had normal calcium levels (76 patients; 77.6%) with the MTHFR C677T genotype variant CC and the MTHFR A1298C genotype variant AA (84 patients; 84.9%). AMPD1 rs17602729 in all patients had a CC genotype with normal calcium levels. The results suggested that there was no significant association between the genetic variation of AMPD1 rs17602729, MTHFR C677T, and MTHFR A1298C with serum calcium levels in patients with RA receiving MTX therapy.
    MeSH terms: Adolescent; Adult; Alleles; Female; Genotype; Humans; Indonesia/epidemiology; Male; Middle Aged; Retrospective Studies; Antirheumatic Agents/therapeutic use; Polymorphism, Single Nucleotide*; Young Adult
  3. Rassem HH, Khamidun MHB, Siyal AA, Mohamed RMSR
    Sci Rep, 2025 Jan 10;15(1):1593.
    PMID: 39794445 DOI: 10.1038/s41598-025-86286-3
    Microwave extraction of active compounds from plants offers an efficient alternative to traditional methods, highlighting the need to investigate its modeling and kinetic mechanisms for comprehensive understanding. This study evaluated kinetic models for the microwave-assisted hydrodistillation (MAHD) of ethanimidic acid from hibiscus flowers. Experimental data, including ethanimidic acid concentration as a function of time, power, and solid-to-liquid ratio, were analyzed using three-parameter empirical models. The second-order model demonstrated superior accuracy (R² = 0.954), outperforming the first-order model, and effectively described the two-stage extraction process: washing and diffusion. The second-order kinetic model ensures precise control of ethanimidic acid extraction, enabling efficient scalability for industrial production and consistent quality for pharmacological applications, emphasizing sustainable and cost-effective manufacturing practices. This model provides a robust framework for advancing MAHD applications in the industrial production of bioactive compounds, promoting innovation in sustainable technologies, and enhancing the economic feasibility of bioactive compound recovery.
    MeSH terms: Kinetics; Microwaves*; Plant Extracts/chemistry; Distillation/methods
  4. Khan AJ, Riyadh SR, Alam MK, Ahmad MS, Sabri BAM, Iqbal A
    PMID: 39794657 DOI: 10.1007/s40368-024-00982-8
    PURPOSE: People with sensory impairments (SI) face unique challenges in out-of-home care, especially in oral health (OH) care, compared to the general population. Various assistive technologies (ATs) and media are used to influence OH behaviors and outcomes for individuals with SI. This systematic review (SR) aimed to identify the types of ATs and assess their effectiveness for individuals with SI.

    METHODS: A comprehensive search strategy was applied across Scopus, PubMed, Cochrane Library, ProQuest, and Scopus databases, using expert-validated keywords, with manual searches for relevant articles published from June 2013 to June 2023. The SR followed the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. Eligible studies focused on the use of ATs as OH education tools for individuals with SI and assessed their impact on OH behavior and status.

    RESULTS: Eighteen of the 1298 identified studies were included in the data synthesis. ATs, such as images, software, multimedia, audio, video, audio-tactile performance, and combination methods, were effective for OH care in individuals with SI. However, this SR limitations include study heterogeneity in sample sizes, intervention types, and outcomes, as well as the exclusion of non-English studies.

    CONCLUSIONS: Oral health education using a combination of video training and audio-tactile performance is particularly effective for individuals with SI, engaging multiple senses for learning. Future research should focus on standardizing methodologies and incorporating larger sample sizes to enhance the efficacy of these technologies in improving OH care for individuals with SI. PROSPERO REG. NO.: CRD42023485523.

  5. Mokhtar AH, Zin RMWM, Yahya A, Zain FM, Selamat R, Ishak Z, et al.
    BMC Public Health, 2025 Jan 10;24(Suppl 1):3626.
    PMID: 39794750 DOI: 10.1186/s12889-024-20726-z
    BACKGROUND: Childhood obesity has increased rapidly in recent years and is now a global epidemic. To combat this, MyBFF@school program, a multi-faceted obesity intervention incorporating physical activity in the form of small-sided games (SSG), nutrition, and psychology components for schoolchildren was designed. This paper is aimed at describing the protocol of the MyBFF@school program and presenting the baseline findings including the overweight and obesity prevalence.

    METHODS: MyBFF@school is a school-based, cluster randomized controlled trial (C-RCT) study. The investigators selected government schools from Federal Territory of Kuala Lumpur, Selangor and Negeri Sembilan by stratified proportionate random sampling based on the multi-ethnic population and the urban-rural location of schools. Subsequently, the schools were assigned randomly to intervention and control groups. The intervention schools underwent MyBFF@school program, whereas the control followed standard school curriculum for a duration of six months. The intervention modules replaced the existing two physical education classes and one co-curriculum activity per week. Three assessments i.e. at baseline, month-3 and month-6 were conducted. Anthropometric, clinical examination, blood, physical fitness, nutrition, and psychology parameters were collected.

    RESULTS: Twenty-three out of 1,196 primary schools (seven interventions and 16 controls) and 15 out of 416 secondary schools (six interventions and nine controls). The investigators screened 11,950 primary (age 9-11 years) and 10,866 secondary (age 13, 14, 16 years) schoolchildren. The investigators found 3,516 primary schoolchildren (29.4%) and 2,910 secondary schoolchildren (26.8%) had BMI z-score of more than + 1SD who were eligible for the study. Of these, 39.7% (N = 1397) of the primary and 35.8% (N = 1041) of the secondary schoolchildren agreed to participate in the study. The mean (SD) characteristics for the participating primary and secondary schoolchildren were: BMI z-score, + 2.29 (± 0.81) and + 2.10 (± 0.71); waist circumference, 75.06 (± 9.6) cm and 85.5 (± 10.9) cm; percentage body fat, 37.8% (± 6.5%) and 39.2% (± 7.3%); and muscle mass, 14.7 (± 2.9) and 23.1 (± 5.2) kg respectively.

    CONCLUSION: MyBFF@school program, a school-based multi-pronged intervention was designed to combat childhood obesity. Screening of 22,816 primary and secondary schoolchildren found 29.4% of primary schoolchildren and 26.8% of secondary schoolchildren to be overweight and obese which reflected the urgency for an effective intervention.

    TRIAL REGISTRATION: Clinical trial number: NCT04155255, November 7, 2019 (Retrospective registered). National Medical Research Register: NMRR-13-439-16563. Registered July 23, 2013. The intervention program was approved by the Medical Research and Ethics Committee (MREC), Ministry of Health Malaysia and Educational Planning and Research Division (EPRD), Ministry of Education Malaysia. It was funded by the Ministry of Health Malaysia.

    MeSH terms: Adolescent; Child; Female; Health Promotion/methods; Humans; Malaysia; Male; School Health Services*; Schools; Program Evaluation; Exercise
  6. Rogers AB, Kale V, Baldi G, Alberdi A, Gilbert MTP, Gupta D, et al.
    Database (Oxford), 2025 Jan 11;2025.
    PMID: 39797569 DOI: 10.1093/database/baae112
    The HoloFood project used a hologenomic approach to understand the impact of host-microbiota interactions on salmon and chicken production by analysing multiomic data, phenotypic characteristics, and associated metadata in response to novel feeds. The project's raw data, derived analyses, and metadata are deposited in public, open archives (BioSamples, European Nucleotide Archive, MetaboLights, and MGnify), so making use of these diverse data types may require access to multiple resources. This is especially complex where analysis pipelines produce derived outputs such as functional profiles or genome catalogues. The HoloFood Data Portal is a web resource that simplifies access to the project datasets. For example, users can conveniently access multiomic datasets derived from the same individual or retrieve host phenotypic data with a linked gut microbiome sample. Project-specific metagenome-assembled genome and viral catalogues are also provided, linking to broader datasets in MGnify. The portal stores only data necessary to provide these relationships, with possible linking to the underlying repositories. The portal showcases a model approach for how future multiomics datasets can be made available. Database URL:  https://www.holofooddata.org.
    MeSH terms: Gastrointestinal Microbiome; Animals; Salmon/microbiology; Databases, Genetic; Microbiota
  7. Mohd Khairuddin AN, Kang J, Gallagher JE
    J Public Health Dent, 2025 Jan 11.
    PMID: 39797710 DOI: 10.1111/jphd.12661
    OBJECTIVE: To evaluate the effect of childhood dental attendance pattern on self-rated oral health in middle adulthood among the British population.

    METHODS: Data from the 1970 British Cohort Study involving participants born in England, Scotland, and Wales were used. Self-rated oral health was assessed at age 46. Childhood dental attendance patterns, derived from follow-up surveys at ages 5, 10, and 16, were classified as 'always', 'sometimes', or 'never' regular. Logistic regression was performed to estimate the effect of childhood dental attendance on adult oral health. Subgroup analyses by education level and oral hygiene practices examined potential variations across these factors.

    RESULTS: This study analyzed data from 4699 participants. An association was found between childhood dental attendance pattern and self-rated oral health in middle adulthood. The odds of reporting poor oral health were higher among those with 'sometimes' and 'never' regular dental attendance patterns than 'always' regular attenders. However, this association became insignificant after adjusting for covariates. Subgroup analyses revealed that 'sometimes' and 'never' regular attendance patterns were associated with higher odds of reporting poor oral health among participants who brushed less than twice daily and those with lower parental academic qualifications.

    CONCLUSION: This study suggests that childhood dental attendance is associated with perceived adult oral health, though this relationship diminished after adjusting for covariates. However, the association persisted among those who brushed less than twice daily and had lower parental academic qualifications. It underscores the importance of both demographic factors and health-related behaviors in determining long-term oral health outcomes.

  8. Looi AD, Palanisamy UD, Moorthy M, Radhakrishnan AK
    Nutr Rev, 2025 Feb 01;83(2):307-328.
    PMID: 38916919 DOI: 10.1093/nutrit/nuae061
    CONTEXT: Vitamin E, a well-known antioxidant with numerous positive effects on human health, encompasses tocotrienol-rich fraction (TRF), a natural variant abundant in palm oil.

    OBJECTIVE: This systematic review analyzed findings from randomized controlled trials published until 2022 to evaluate the health impacts of palm TRF.

    DATA SOURCES: A literature search was performed in Cochrane Central Register of Controlled Trials (CENTRAL), PubMed, OVID Medline, SCOPUS, and Web of Science from inception until December 2022. Thirty studies involving 2646 patients, including both healthy individuals and those with underlying conditions, were identified.

    RESULTS: This review shows palm TRF to be a promising natural supplement against inflammation and lipid peroxidation and that can significantly enhance overall health. Additionally, the study underscores the necessity for further research to ascertain the optimal dosage, formulation, and duration of supplementation, maximizing the potential health advantages.

    CONCLUSION: This systematic review provides evidence supporting the health benefits associated with palm TRF.

    SYSTEMATIC REVIEW REGISTRATION: PROSPERO registration no. CRD42020204070.

    MeSH terms: Humans; Lipid Peroxidation/drug effects; Randomized Controlled Trials as Topic*; Dietary Supplements*
  9. Shaharir SS, Nawi AM, Mariamutu TN, Kamaruzaman L, Said MSM, Rajalingham S, et al.
    Int J Rheum Dis, 2025 Jan;28(1):e70043.
    PMID: 39791506 DOI: 10.1111/1756-185X.70043
    OBJECTIVES: To determine the prevalence of self-reported delayed adverse events (DAEs), major AEs, and flares following COVID-19 vaccinations among patients with autoimmune rheumatic diseases (AIRDs) in Malaysia.

    METHODOLOGY: An electronically validated survey from the COVID-19 vaccination in autoimmune diseases (COVAD) study group was distributed in July 2021 to patients with autoimmune diseases and healthy controls (HCs). The survey collected data on DAEs (any AE that persisted or occurred after 7 days of vaccination), any early or delayed major adverse events (MAEs), and flares following COVID-19 vaccination. Generalized estimating equation (GEE) models were performed to determine the factors associated with repeated events of DAEs, MAEs, and flares.

    RESULTS: A total of 556 vaccines were administered to 204 subjects (150 AIRDs and 54 HCs), with 72.1% completing 3 doses. In multivariate GEE analysis, there was a greater frequency of minor DAEs among AIRDs versus HCs (OR 5.65, p = 0.052). The occurrence of MAEs was higher in AIRDs versus HCs (4.9% vs. 1.3%, p = 0.052), but it was no longer significant in the GEE model. In the AIRDs group, the BNT162b2 vaccine increased the risk for minor DAEs (OR4.68, p = 0.02) while patients with autoimmune multimorbidity showed a greater risk for MAEs (OR 8.25, p = 0.007). The rate of flare was 10.6% and multivariate GEE analysis revealed that The rate of flare was 10.6% and multivariate GEE analysis revealed that systemic lupus erythematosus (SLE) (OR0.31, p = 0.03) and hydroxychloroquine (HCQ) (OR 0.16, p 

    MeSH terms: Symptom Flare Up; Adult; Aged; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Risk Factors; Time Factors; Vaccination/adverse effects; Case-Control Studies; Self Report*
  10. Safriansyah W, Juliansyah E, Rustaman, Naini AA, Farabi K, Azmi MN, et al.
    J Asian Nat Prod Res, 2025 Jan 10.
    PMID: 39791902 DOI: 10.1080/10286020.2024.2446280
    Aglaia pachyphylla is a species from the Aglaia genus (Meliaceae) and the chemical constituent has not been widely explored. A new cycloartane-type triterpenoid, pachyphyllanone (1), along with four known compounds (2-5) were isolated from Aglaia pachyphylla Miq. Furthermore, the structure of the new compound was elucidated by the interpretation of spectroscopic data, including 1D and 2D-NMR, as well as ECD and NMR calculations (DP4+ analysis). Compounds 1-5 showed cytotoxic activities against MCF-7 with IC50 values ranging from 160.74 to 299.75 μM.
  11. Naqvi SAH, Farhan M, Ahmad M, Kiran R, Shahbaz M, Abbas A, et al.
    Arch Microbiol, 2025 Jan 10;207(2):31.
    PMID: 39792175 DOI: 10.1007/s00203-024-04219-6
    The agricultural productivity and world-wide food security is affected by different phytopathogens, in which Fusarium is more destructive affecting more than 150 crops, now got resistance against many fungicides that possess harmful effects on environment such as soil health, air pollution, and human health. Fusarium fungicide resistance is an increasing concern in agricultural and environmental contexts, requiring a thorough understanding of its causes, implications, and management approaches. The mechanisms of fungicide resistance in Fusarium spp., are reviewed in this article, including increased efflux pump activity, target-site mutations, and metabolic detoxification pathways. Fusarium is naturally resistant to some of the fungicides, on the other hand; it speedily develops resistance against the other fungicides groups. Most of the important plant pathogenic Fusarium species including F. oxysporum, F. psedogramanium, F. graminearium and Fusarium solani, which have shown resistance to major groups of fungicides including triazoles, phenylpyrole and benzimedazoles in various regions of the world. The review also covers a range of management techniques, including fungicide rotation, resistant cultivars, cultural methods, and biological control agents, to lessen fungicide resistance. By shedding light on the current state of knowledge concerning fungicide resistance in Fusarium spp., this review provides valuable information to researchers, policymakers, and practitioners to design long-term effective disease management approaches, as well as fungal menace control to preserve fungicides' effectiveness in agriculture and conservancy activities.
    MeSH terms: Agriculture; Crops, Agricultural/microbiology; Drug Resistance, Fungal*
  12. Huang RY, Zhang C, Lim HL
    Curr Microbiol, 2025 Jan 10;82(2):78.
    PMID: 39792222 DOI: 10.1007/s00284-024-04057-1
    Staphylococcus epidermidis (S. epidermidis) live in different human locations and natural environments. For ribotyping S. epidermidis sub-species, 2507 PCR-amplified reads of 16S rRNA genes of S. epidermidis in a public dataset were used for probabilistic sequence analysis. A sequence probability logo (sequence pLogo) as a reference sequence of 16S rRNA genes of S. epidermidis was constructed. Through implementation of Levenshtein Distance algorithm, two 20-base pairs (bp) motifs, commonly present in 2507 PCR-amplified reads, were identified. The top 38 S. epidermidis isolates, which carried 16S rRNA nucleotide domains that were made of different sequences but have high similarity scores to two 20-bp motifs, were found from 11 human, 8 animal, 9 plant and 10 environmental samples, indicating that these two 20-bp motifs were broadly present in diverse S. epidermidis isolates. Thirty-one PCR-amplified reads of 16S rRNA genes, which were currently not in the dataset, were utilized to verify the feasibility of using two 20-bp motifs for ribotyping S. epidermidis sub-species. S. epidermidis S1, S3, but not S2, isolates on the human scalp carried a 20-bp sequence domain with high similarities to a 20-bp motif in the sequence pLogo. The phylogenetic tree showed that S. epidermidis S1, S2 and S3 were not from a single common ancestor. Two newly identified 20-bp motifs here, thus, provided reference nucleotide residues for ribotyping S. epidermidis.
    MeSH terms: Algorithms*; Animals; DNA, Bacterial/genetics; Humans; Phylogeny*; Staphylococcal Infections/microbiology; Sequence Analysis, DNA; Ribotyping*
  13. Yusoff MZM, Ruzlin ANM, Mohamad M, Zainuddin MA
    J Vector Borne Dis, 2025 Jan 10.
    PMID: 39792448 DOI: 10.4103/JVBD.JVBD_127_24
    BACKGROUND OBJECTIVES: Dengue is now endemic in over 100 countries, with Asia bearing over 70% of the global burden. In Malaysia, dengue cases have increased dramatically, particularly in Pulau Pinang, where cases rose from 1,621 in 2022 to 7,343 in 2023. To examine factors associated with dengue outbreaks in Pulau Pinang in 2023 by comparing outbreak cases with single cases.

    METHODS: Cross-sectional study using eDengue database, the Malaysian National Dengue Registry.

    RESULTS: The study included 1,106 confirmed dengue cases, with 553 categorised as outbreak cases and 553 as single cases. Significant associations were found between dengue outbreaks and Priority Locality 1 (areas with Uncontrolled Outbreaks/ Hotspots/ Recurrent Outbreaks/ Dengue Deaths in the previous year) (AOR: 0.15, 95% CI: 0.06,0.37, p<0.001), Aedes Index ≥1% (AOR: 1.35, 95% CI: 1.02,1.77, p=0.031), patients initially visiting government clinics (AOR: 1.63, 95% CI: 1.10,2.43, p=0.015), hospitalized dengue patients (AOR: 0.70. 95%CI: 0.52,0.96, p=0.029), the time taken from notification to source reduction (AOR: 0.57, 95% CI: 0.50,0.66, p<0.001) and time taken from notification to investigation (AOR: 2.40 95%CI: 1.77,3.24, p<0.001).

    INTERPRETATION CONCLUSION: This study identifies key factors associated with dengue outbreaks in Pulau Pinang such as Aedes Index, Priority Locality 1 areas and initial visits to government clinics. These findings underscore the need for targeted vector control and early intervention in high-risk areas to reduce outbreak risk. Early healthcare seeking behavior and timely intervention are critical in managing the spread of dengue. Further research is needed to explore additional factors and improve public health strategies.

  14. Bosticardo M, Dobbs K, Delmonte OM, Martins AJ, Pala F, Kawai T, et al.
    Sci Immunol, 2025 Jan 10;10(103):eadq1697.
    PMID: 39792639 DOI: 10.1126/sciimmunol.adq1697
    Human recombination-activating gene (RAG) deficiency can manifest with distinct clinical and immunological phenotypes. By applying a multiomics approach to a large group of RAG-mutated patients, we aimed at characterizing the immunopathology associated with each phenotype. Although defective T and B cell development is common to all phenotypes, patients with hypomorphic RAG variants can generate T and B cells with signatures of immune dysregulation and produce autoantibodies to a broad range of self-antigens, including type I interferons. T helper 2 (TH2) cell skewing and a prominent inflammatory signature characterize Omenn syndrome, whereas more hypomorphic forms of RAG deficiency are associated with a type 1 immune profile both in blood and tissues. We used cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) analysis to define the cell lineage-specific contribution to the immunopathology of the distinct RAG phenotypes. These insights may help improve the diagnosis and clinical management of the various forms of the disease.
    MeSH terms: B-Lymphocytes/immunology; Child, Preschool; DNA-Binding Proteins/deficiency; DNA-Binding Proteins/genetics; DNA-Binding Proteins/immunology; Female; Humans; Male; Mutation; Nuclear Proteins; Phenotype; Homeodomain Proteins/genetics; Homeodomain Proteins/immunology
  15. Tiwari S, Sharma AK, Abdul Aziz I, Gupta D, Jain A, Mahdin H, et al.
    PLoS One, 2025;20(1):e0315135.
    PMID: 39792787 DOI: 10.1371/journal.pone.0315135
    Texture is a significant component used for several applications in content-based image retrieval. Any texture classification method aims to map an anonymously textured input image to one of the existing texture classes. Extensive ranges of methods for labeling image texture were proposed earlier. However, computing the performance of these methods in the presence of various degradations is always an open area of discussion. Image noise is always a dominant factor among various image degradation factors, affecting the performance of these methods and making texture classification challenging. Therefore, it is essential to investigate the interpretation of these methods in the presence of prominent degradation factors such as noise. Applications for Segmentation-Based Fractal Texture Features (SFTF) include image classification, texture generation, and medical image analysis. They are beneficial for examining textures with intricate, erratic patterns that are difficult to characterize using conventional statistical techniques accurately. This paper assesses two texture feature extraction methods based on SFTF and statistical moment-based texture features in the presence and absence of Gaussian noise. The SFTF and statistical moments-based handcrafted features are passed to a multilayer feed-forward neural network for classification. These models are evaluated on natural textures from Kylberg Texture Dataset 1.0. The results show the superiority of segmentation-based fractal analysis over other approaches. The average accuracy rates using the SFTF are 99% and 97% in the absence and presence of Gaussian noise, respectively.
    MeSH terms: Algorithms; Humans; Normal Distribution; Neural Networks (Computer); Fractals*
  16. Adam RI, Rajaratnam S, Sufian FD, Njogu L
    PLoS One, 2025;20(1):e0314284.
    PMID: 39792835 DOI: 10.1371/journal.pone.0314284
    Gender equality and women's empowerment have been increasingly emphasised in food production systems, including fisheries and aquaculture. Accurate assessment and understanding of the state, progress and changes in women's empowerment in the sub-sectors is required. We applied the project level Women's Empowerment in Fisheries and Aquaculture Index (pro-WEFI), which is based on the project-level women's empowerment in agriculture index (pro-WEAI) to standardize the measurement of women's agency and empowerment in fisheries and aquaculture. Drawing on a survey conducted in north-western Bangladesh, we examined quantitative pro-WEFI data collected from 217 households engaged in aquaculture. Only 33% of the women and 48% of the men in the sample achieved empowerment in aquaculture, attaining scores of 0.75 and above. The mean disempowerment score (1-3DE) revealed that both women and men failed to achieve adequacy on average in nearly 28% of the indicators. Nearly 40% of the dual adult households did not attain gender parity with women achieving lower adequacy scores than men from the same household. Women's disempowerment was primarily driven by lack of autonomy in their use of income (18.5%), inability to visit important locations (17.4%), and inadequate access to and decision making on financial services (13.4%). Our findings emphasize the significance of conducting comprehensive assessments of women's empowerment in aquaculture initiatives and its various domains and indicators inform the development of targeted and effective interventions. By identifying domains where gender inequality is most pronounced, projects can better design interventions to create targeted impacts in critical areas.
    MeSH terms: Adult; Bangladesh; Family Characteristics; Female; Fisheries*; Humans; Male; Middle Aged; Power (Psychology); Surveys and Questionnaires; Women's Rights; Aquaculture*
  17. Suhaizan FS, Mohd Taib A, Taha MR, Hasbollah DZA, Ibrahim A, Dan MFM, et al.
    PLoS One, 2025;20(1):e0316488.
    PMID: 39792898 DOI: 10.1371/journal.pone.0316488
    Rainfall-induced landslides are a frequent geohazard for tropical regions with prevalent residual soils and year-round rainy seasons. The water infiltration into unsaturated soil can be analyzed using the soil-water characteristic curve (SWCC) and permeability function which can be used to monitor and predict incoming landslides, showing the necessity of selecting the appropriate model parameter while fitting the SWCC model. This paper presents a set of data from six different sections of the studied slope at varying depths that are used to test the performance of three SWCC models, the van Genuchten-Mualem (vG-M), Fredlund-Xing (F-X) and Gardner (G). The dataset is obtained from field monitoring of the studied slope, over a duration of 6 months. The study discovered that the van Genuchten-Mualem model provided the best estimation based on RMSE and evaluation metric, R2 followed by Fredlund and Xing, and Gardner, however, the difference between them is minor. The R2 obtained varies as the value at the crest with 1.0 m depth has a mean of 0.44, the lowest among the overall data fitted but it also has the best RMSE value with a mean of 0.00473. Whereas the location mid-section at a depth of 1.0 m has the highest R2 with a mean of 0.97, and an average value of RMSE of 0.0145 which is the middle of the group that was fitted. This indicates that R2 measurement for model performance relies highly on the dispersion of the variables collected. The dispersion of the data set is mainly due to the sensors' inability to detect effectively at exceedingly high matric suction and zero matric suction. The investment in improving the equipment's precision will boost reliability and reduce the number of assumptions as the data is collected from the site rather than laboratory testing.
    MeSH terms: Malaysia; Models, Theoretical; Rain; Water*; Landslides
  18. See SA, Bhassu S, Tang SS, Yusoff K
    Dev Comp Immunol, 2025 Jan;162:105264.
    PMID: 39299363 DOI: 10.1016/j.dci.2024.105264
    White spot syndrome virus (WSSV) causes highly destructive infection in crustacean aquaculture, often resulting in 100% mortality within a week. However, there is lack of studies addressing the safety issues of WSSV vaccines in shrimps. In this study, WSSV VP28 mRNA vaccines were developed using codon deoptimization approach. These vaccines were administered to Litopenaeus vannamei shrimps at various dosages to access their safety and the shrimps' immune responses using quantification PCR (qPCR). The findings of this study indicate that the expression level of codon deoptimized VP28 mRNA vaccines are lower compared to the wild type VP28 vaccines, as observed through a comparison of bioinformatic predictions and experimental results. Additionally, the total haemocyte count (THC) in shrimps injected with codon deoptimized VP28 vaccine was higher than those injected with wild type VP28 vaccines. Furthermore, the expression of immune-related genes differed between codon deoptimized and wild type VP28 vaccines. In summary, the results suggest that 0.01 μg codon deoptimized VP28-D1 mRNA vaccine is the most promising WSSV mRNA vaccine, displaying low pathogenicity and expression in shrimps. To the best of our knowledge, this research represents the first attempt to attenuate WSSV using codon deoptimization method and development of a potential mRNA vaccine for shrimp purpose. The study addresses an important gap in shrimp vaccine research, offering potential solutions for WSSV control in shrimps.
    MeSH terms: Animals; DNA Virus Infections/immunology; Vaccination*; Viral Envelope Proteins; Aquaculture
  19. Thian BYZ, Fatimah MNN, Wong CL, Ong HK, Mariatulqabtiah AR, Ho KL, et al.
    Dev Comp Immunol, 2025 Jan;162:105275.
    PMID: 39341478 DOI: 10.1016/j.dci.2024.105275
    Avian influenza A viruses (IAVs) pose a persistent threat to poultry industry worldwide, despite the presence of vaccines. Additionally, reverse-zoonosis transmission potentially introduces human-originated IAVs into poultry and complicates the efforts to control the spread of influenza. Current avian influenza vaccines are primarily based upon the rapidly mutating hemagglutinin (HA) and neuraminidase (NA) glycoproteins, which limit their efficacy against diverse strains of IAVs. Hence, the highly conserved ectodomains of matrix 2 protein (M2e) of IAVs are widely studied as alternatives to the HA and NA. However, the differences in the M2e amino acid sequences between avian and human IAVs generate antibodies that do not cross-react reciprocally with IAVs from other origins. To broaden and enhance the immunogenicity of M2e, we fused two copies each of the M2e derived from avian and human IAVs at the C-terminal end of the Macrobrachium rosenbergii nodavirus (MrNV) capsid protein (NvC). Transmission electron microscopic and dynamic light scattering analyses revealed that the chimeric protein self-assembled into virus-like particles (VLPs). Immunization of chickens with the chimeric VLPs demonstrated a robust induction of broadly reactive immune responses against both the M2e of avian and human IAVs. Additionally, the chimeric VLPs elicited the production of cytotoxic T lymphocytes (CTL), macrophages, as well as a well-balanced Th1 and Th2 population, indicating their potential in activating cell-mediated immune responses in chickens. Furthermore, the chimeric VLPs triggered the production of both Th1- and Th2-cytokines, attesting their potential in mounting a robust and balanced immune response in avian species. This study demonstrated the potential of these chimeric VLPs in stimulating and broadening cross-reactive immune responses in chickens against both avian and human IAVs.
    MeSH terms: Animals; Cross Reactions*; Humans; Immunization; Influenza, Human/immunology; Influenza, Human/prevention & control; Influenza, Human/virology
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