Browse publications by year: 2025

  1. Mohd Sahardi NFN, Priya M, Makpol S, Shafiee MN
    J Obstet Gynaecol Res, 2025 Mar;51(3):e16246.
    PMID: 40015330 DOI: 10.1111/jog.16246
    OBJECTIVE: This comprehensive review highlights the current research on metabolomics and the metabolic pathways involved in endometrial cancer (EC), offering potential non-invasive biomarkers for EC.

    METHODS: The data was extracted from published manuscripts between 2015 and 2024 using the reputed search engine "Pubmed." All gathered data were organized into a single table, facilitating a comparison with earlier findings.

    RESULTS: The results of this study revealed most metabolites identified in previous metabolomic research on EC are associated with lipid, glucose, and amino acid metabolism.

    CONCLUSION: Therefore, understanding these metabolic pathway alterations in EC is crucial for improving diagnosis, prognosis, and treatments by specially targeting these metabolic pathways.

    MeSH terms: Female; Humans; Metabolomics*
  2. Yusoff MHM, Shafie MH
    Int J Biol Macromol, 2025 Feb 25.
    PMID: 40015410 DOI: 10.1016/j.ijbiomac.2025.141469
    Deep eutectic solvents (DES) have emerged as promising solvents for polysaccharide extraction from various sources. The DES which is produced by combining hydrogen bond donors and acceptors offers sustainability, low toxicity, a wide range of solubility and tailored properties. This review examines DES features and their effectiveness as extraction media for polysaccharides, highlighting the mechanisms behind their enhanced extraction efficiency compared to classical solvents. Additionally, we discuss the mechanism behind the DES affecting the physicochemical and structural properties of the extracted polysaccharides. The review also explores the antioxidant, antihyperglycemic, antihyperlipidemic and immunomodulatory properties of DES-extracted polysaccharides compared to classical solvents which emphasize structural changes in the polymer complex. This review intends to shed insight into the prospects of green extraction technologies by providing information on the benefits of DES and its potential to modify polysaccharide characteristics and enhance their biological activities, which is covered in depth for the first time here.
  3. Wang X, Abu Bakar MH, Liqun S, Kassim MA, Shariff KA, Karunakaran T
    J Ethnopharmacol, 2025 Mar 26;344:119560.
    PMID: 40015541 DOI: 10.1016/j.jep.2025.119560
    ETHNOPHARMACOLOGICAL RELEVANCE: Tripterygium wilfordii is a traditional Chinese medicine used to treat rheumatic diseases, with properties such as clearing heat, detoxifying, dispelling wind, and relieving pain. In recent years, its active compound, celastrol, garnered significant attention for its potential therapeutic effects on metabolic diseases. Celastrol exhibits bioactivities such as regulating metabolic functions and anti-inflammatory effects, positioning it as a promising candidate for the treatment of obesity, diabetes, atherosclerosis (AS), and non-alcoholic fatty liver disease (NAFLD).

    AIM OF THE REVIEW: This review aims to explore the pharmacological mechanisms of celastrol in metabolic diseases, focusing on its anti-inflammatory mechanisms and metabolic regulation effects, providing theoretical support for further investigation of its therapeutic potential in metabolic diseases.

    METHODS: Literature was retrieved from PubMed, Web of Science, Scopus, Cochrane, and Google Scholar. This review primarily focuses on anti-inflammatory mechanisms of celastrol, its metabolic regulation, and toxicity studies, by systematically analyzing its effects in obesity, diabetes, AS, and NAFLD, providing scientific evidence for its potential clinical applications.

    RESULTS: Celastrol regulates multiple signaling pathways, particularly inhibiting NF-κB and activating AMPK, reducing the production of pro-inflammatory cytokines and improving insulin sensitivity, enhancing its therapeutic potential in metabolic diseases. Additionally, celastrol regulates adipogenesis and energy metabolism by influencing key transcription factors such as PPARγ and SREBP-1c. Numerous studies highlight its role in alleviating oxidative stress and improving mitochondrial function, further enhancing its metabolic benefits.

    CONCLUSION: In summary, celastrol holds great promise as a multi-target therapeutic agent for metabolic diseases, offering anti-inflammatory, metabolic regulatory, and antioxidative benefits. Despite these, challenges remain for the clinical application of celastrol due to its poor bioavailability and potential toxicity. Advanced formulation strategies and targeted delivery systems are urgently needed to overcome challenges related to bioavailability and clinical translation.

    MeSH terms: Animals; Humans; Tripterygium/chemistry
  4. Ye Y, Gu Z, Geng J, Niu K, Yu P, Zhou Y, et al.
    Nano Lett, 2025 Mar 12;25(10):3747-3755.
    PMID: 40015691 DOI: 10.1021/acs.nanolett.4c05460
    Stable solid electrolytes are essential for advancing the safety and energy density of lithium batteries, especially in high-voltage applications. In this study, we designed an innovative high-entropy chloride solid electrolyte (HE-5, Li2.2In0.2Sc0.2Zr0.2Hf0.2Ta0.2Cl6), using multielement doping to optimize both ionic conductivity and high-voltage stability. The high-entropy disordered lattice structure facilitates lithium-ion mobility, achieving an ionic conductivity of 4.69 mS cm-1 at 30 °C and an activation energy of 0.300 eV. Integration of HE-5 into all-solid-state batteries (ASSBs) with NCM83 cathodes and a Li-In anode enables outstanding electrochemical performance, sustaining 70% capacity retention over 1600 cycles at a 4 C rate. Moreover, the high configurational entropy stabilizes the electrolyte's structure at elevated voltages, enabling stable operation at 5.0 V without significant degradation. Our work presents the dual advantages of high-entropy engineering in boosting high ionic conductivity and voltage stability, providing a broad roadmap for next-generation energy-dense ASSBs.
  5. Lee KKC, Mohamed NNE, Abdul Aziz S, Mohamad Nazri D, Abdul Malek AZ, Lim JS, et al.
    Expert Opin Pharmacother, 2025 Apr;26(5):653-662.
    PMID: 40015692 DOI: 10.1080/14656566.2025.2472976
    BACKGROUND: Major depressive disorder (MDD) is a growing global health concern, with its economic impact projected to reach USD 6.0 trillion by 2030. Treatment-resistant depression (TRD), characterized by non-responsiveness to initial treatments, adds a significant cost burden. In Malaysia, the National Health and Morbidity Survey 2023 reported a rising prevalence of depression in Malaysia, affecting approximately one million individuals.

    RESEARCH DESIGN AND METHODS: A combined retrospective chart review and cross-sectional survey was conducted at the Psychiatry and Mental Health Clinic of Hospital Kuala Lumpur (HKL) over an 18-month period, examining 286 patients diagnosed with MDD or TRD. Data were analyzed from governmental and societal perspectives, including direct medical costs, indirect costs, and other resource utilization.

    RESULTS: TRD patients exhibited significantly higher health-care utilization, including a fivefold increase in the likelihood of attending more than 10 consultations (p = 0.035) and greater use of second-generation antipsychotics (p 

    MeSH terms: Adult; Aged; Antidepressive Agents/economics; Antidepressive Agents/therapeutic use; Cross-Sectional Studies; Female; Health Resources/economics; Health Resources/statistics & numerical data; Humans; Malaysia/epidemiology; Male; Middle Aged; Quality of Life*; Retrospective Studies; Cost of Illness; Young Adult
  6. Lee EH, Roberts AC, Kwok KW, Car J, Soh CK, Christopoulos G
    Sci Rep, 2025 Feb 28;15(1):7107.
    PMID: 40016504 DOI: 10.1038/s41598-025-89590-0
    Underground office workspaces (UOW) have emerged as a sustainable option in land-scarce megacities. Yet, the main challenge is to design and manage underground spaces that cater to the psychological needs of occupants. By integrating insights from social psychology, urban studies, and policy making, we identify and provide evidence for a key psychological mechanism underlying the adoption of UOW: "locus of control", a psychological process that reflects whether people feel in control of their environment or see it as shaped by external forces. Study 1 (N = 1093) revealed that individuals who believe external forces strongly impact their lives tend to have more negative attitudes toward working at UOW, perceiving negative aspects of the underground environment (e.g., confinement) as more salient. Study 2 (N = 217) builds on the findings of Study 1 by demonstrating that individuals who feel strongly influenced by external forces actively avoid UOW as their workplace, as evidenced by differences in locus of control between current underground and aboveground office workers. The present findings uncover a key psychological mechanism that can facilitate the adoption of UOW and invites engineers, designers and management to develop UOW environments that promote a sense of control and autonomy, fostering sustainable and occupant-friendly urban infrastructure.
    MeSH terms: Adult; Cities*; Female; Humans; Internal-External Control; Male; Middle Aged
  7. Sambasivam G, Prabu Kanna G, Chauhan MS, Raja P, Kumar Y
    Sci Rep, 2025 Feb 27;15(1):7009.
    PMID: 40016508 DOI: 10.1038/s41598-025-90646-4
    Detecting cassava leaf disease is challenging because it is hard to identify diseases accurately through visual inspection. Even trained agricultural experts may struggle to diagnose the disease correctly which leads to potential misjudgements. Traditional methods to diagnose these diseases are time-consuming, prone to error, and require expert knowledge, making automated solutions highly preferred. This paper explores the application of advanced deep learning techniques to detect as well as classify cassava leaf diseases which includes EfficientNet models, DenseNet169, Xception, MobileNetV2, ResNet models, Vgg19, InceptionV3, and InceptionResNetV2. A dataset consisting of around 36,000 labelled images of cassava leaves, afflicted by diseases such as Cassava Brown Streak Disease, Cassava Mosaic Disease, Cassava Green Mottle, Cassava Bacterial Blight, and healthy leaves, was used to train these models. Further the images were pre-processed by converting them into grayscale, reducing noise using Gaussian filter, obtaining the region of interest using Otsu binarization, Distance transformation, as well as Watershed technique followed by employing contour-based feature selection to enhance model performance. Models, after fine-tuned with ADAM optimizer computed that among the tested models, the hybrid model (DenseNet169 + EfficientNetB0) had superior performance with classification accuracy of 89.94% while as EfficientNetB0 had the highest values of precision, recall, and F1score with 0.78 each. The novelty of the hybrid model lies in its ability to combine DenseNet169's feature reuse capability with EfficientNetB0's computational efficiency, resulting in improved accuracy and scalability. These results highlight the potential of deep learning for accurate and scalable cassava leaf disease diagnosis, laying the foundation for automated plant disease monitoring systems.
    MeSH terms: Image Processing, Computer-Assisted/methods; Plant Leaves*
  8. Subramaniyan V, Hanim YU
    Int J Obes (Lond), 2025 Mar;49(3):492-506.
    PMID: 40016558 DOI: 10.1038/s41366-025-01729-1
    The worldwide health emergency of obesity is closely connected to how dietary fats are metabolized, whereas the process is significantly influenced by pancreatic lipase (PL), an enzyme critical for lipid hydrolysis into fatty acids. This narrative review employs a methodological approach utilizing literature searches of PubMed data up to March 2024. The search term criteria encompasses keywords related to the role, mechanism, challenges, and current and future treatments of pancreatic lipase in obesity with an overall references is 106. This paper offers a comprehensive explanation of the role of PL, underlining its significance in the digestive process and lipid imbalances that contribute to obesity and by extension, its impact on obesity development and progression. Additionally, it delves into the dual functionality of the pancreas, emphasizing its impact on metabolism and energy utilization which, when dysregulated, promotes obesity. A focal point of this review is the investigation into the efficacy, challenges, and adverse effects of current pancreatic lipase inhibitors, with orlistat being highlighted as a primary current drug delivery. By discussing advanced obesity treatments, including the exploration of novel anti-obesity medications that target specific biological pathways, this review underscores the complexity of obesity treatment and the necessity for a multifaceted approach. In conclusion, this paper emphasizing the importance of understanding the role of enzymes like pancreatic lipase mechanistic and adopting a multidisciplinary approach to treatment and side effects of current obesity drugs and explore new emerging therapeutic strategies for more effective obesity management.
    MeSH terms: Humans
  9. Eslam M, Fan JG, Yu ML, Wong VW, Cua IH, Liu CJ, et al.
    Hepatol Int, 2025 Feb 27.
    PMID: 40016576 DOI: 10.1007/s12072-024-10774-3
    Metabolic dysfunction-associated fatty liver disease (MAFLD) affects over one-fourth of the global adult population and is the leading cause of liver disease worldwide. To address this, the Asian Pacific Association for the Study of the Liver (APASL) has created clinical practice guidelines focused on MAFLD. The guidelines cover various aspects of the disease, such as its epidemiology, diagnosis, screening, assessment, and treatment. The guidelines aim to advance clinical practice, knowledge, and research on MAFLD, particularly in special groups. The guidelines are designed to advance clinical practice, to provide evidence-based recommendations to assist healthcare stakeholders in decision-making and to improve patient care and disease awareness. The guidelines take into account the burden of clinical management for the healthcare sector.
  10. Aliza D, Zuki FM, Hassan CRC, Suhendrayatna S, Javanmard A
    Environ Monit Assess, 2025 Feb 28;197(3):338.
    PMID: 40016595 DOI: 10.1007/s10661-025-13755-7
    This study investigates the potential of Escherichia coli and Aeromonas hydrophila as bioremediation agents for removing copper (Cu) and zinc (Zn) from contaminated water. Although Cu and Zn are necessary in trace levels, excessive amounts can be harmful and can linger in aquatic environments, endangering the food chain. Bioremediation using microorganisms offers an alternative method for mitigating heavy metal pollution. In this study, 126 tilapia fish (Oreochromis mossambicus) were exposed to CuSO4 and ZnCl2 for 15 days, followed by treatment with E. coli and A. hydrophila. Atomic absorption spectrometry (AAS) revealed that both bacterial treatments reduced copper and zinc accumulation in fish organs, though they did not fully heal external lesions. Histopathological analysis showed significant reductions in melanomacrophage centers (MMC), cell necrosis, cell dissociation, and vacuolization in fish liver tissue after bacterial treatment, particularly at concentrations of 2.5 mg.L-1 and 5 mg.L-1 for CuSO4 and 7.5 mg.L-1 for ZnCl2. These findings suggest that E. coli and A. hydrophila have the potential to be developed as effective bioremediation agents for CuSO4 and ZnCl2 pollution in aquatic environments.
    MeSH terms: Animals; Biodegradation, Environmental*; Chlorides*; Copper/metabolism; Escherichia coli*; Spectrophotometry, Atomic; Zinc/metabolism; Tilapia*; Copper Sulfate*
  11. Kuesap J, Suphakhonchuwong N, Eksonthi B, Huaihongthong S
    Malar J, 2025 Feb 27;24(1):63.
    PMID: 40016697 DOI: 10.1186/s12936-025-05305-w
    BACKGROUND: The genetic diversity of malaria parasites varies between regions in the world. The genetic polymorphisms of the genes Pvs48/45 and Pvs47 which encode gametocyte/gamete proteins of Plasmodium vivax, were studied because of their potential as transmission-blocking vaccine (TBV) targets. The aim of the present study was to investigate the genetic diversity of Pvs48/45 and Pvs47 in clinical isolates from endemic areas of Thailand.

    METHODS: Plasmodium vivax samples collected from four provinces neighbouring either Myanmar or Malaysia were analysed using polymerase chain reaction and nucleotide sequencing.   RESULTS: Fifteen and 18 amino acid substitutions were observed in 36 Pvs48/45 and 62 Pvs47 deduced amino acid sequences, respectively. Eleven haplotypes were identified in Pvs48/45 and 26 in Pvs47. Overall, low nucleotide diversities were observed for Pvs48/45 (π = 0.00104) and Pvs47 (π = 0.00321). Tajima's D, and Fu and Li's D* and F* values were negative for both genes, Pvs48/45 and Pvs47 while a significant difference was found in Pvs48/45 (P 

    MeSH terms: Antigens, Protozoan/genetics; Antigens, Protozoan/immunology; Humans; Polymorphism, Genetic*; Thailand; Protozoan Proteins/genetics; Polymerase Chain Reaction; Sequence Analysis, DNA
  12. Chua EW, Karunanathie H, Yanasegaran K, Maggo S, Kee PS, Kennedy M, et al.
    BMC Res Notes, 2025 Feb 27;18(1):88.
    PMID: 40016731 DOI: 10.1186/s13104-025-07156-9
    OBJECTIVE: CYP2D6 activity has been inconsistently associated with anxious and depressive personality traits. The inconsistency may stem from limitations of targeted genotyping, employed in most previous studies, leading to undetected errors in metabolic classification. Using a nanopore sequencing-based method, we comprehensively genotyped CYP2D6 alleles in a small cohort of 96 Malaysians and re-examined the relationship between CYP2D6 activity and susceptibility to anxiety and depression.

    RESULTS: In keeping with prior studies, CYP2D6*10 was found to be the most common defective allele. Nearly half of the (48.5%) participants were classified as intermediate and poor metabolizers. Linear regression analysis suggested that impaired CYP2D6 activity could be a predictor of anxiety and depression, consistent with the putative role of CYP2D6 in the synthesis of serotonin and dopamine, the mood-boosting neurotransmitters. We hope this brief report will prompt larger-scale studies to further elucidate the contribution of CYP2D6 to the genetic underpinnings of mental well-being.

    MeSH terms: Adult; Alleles; Female; Genotype; Humans; Male; Middle Aged; Genetic Predisposition to Disease*; Young Adult
  13. Yang W, Duan Y, Liao Z
    BMC Psychol, 2025 Feb 27;13(1):168.
    PMID: 40016851 DOI: 10.1186/s40359-025-02515-4
    PURPOSE: This study aims to explore the specific psychological mechanisms of female victims coping with gender bias in bystander intervention (Study 1) and bystander neglect (Study 2), as well as the influence of bystander gender.

    METHODOLOGY: Two experiments recruited 208 participants who, after watching a first-person video of their experience of gender prejudice, filled out questionnaires measuring emotions, feelings of power, evaluation of the perpetrator and willingness to confront. A moderated mediation model was set up, and the bootstrapping method were applied.

    FINDINGS: We found that the victim's feeling of power significantly mediated the relationship between anger and confrontation intention in bystander intervention. The victim's negative evaluation of the perpetrator significantly mediated between anger and confrontation intention in bystander neglect. Notably, both mediation models could be constructed only when the bystander was male rather than female.

    IMPLICATIONS: This study has important implications for gender bias. The results reveal the psychological mechanism of victims coping with gender bias, and call for groups of all genders to join in the anti-gender bias alliance.

    MeSH terms: Adaptation, Psychological; Adolescent; Adult; Anger; Female; Humans; Male; Middle Aged; Power (Psychology); Surveys and Questionnaires; Young Adult
  14. Shahrul AI, Nik Mustapha NM, Ahmad MS, Kharbanda OP, Abd Rahman ANA
    PMID: 40017125 DOI: 10.1177/10556656241285808
    OBJECTIVE: To develop a core outcome set for the audit of cleft care in Malaysia.

    DESIGN: To generate a core outcome set for patients dealing with cleft lip and/or palate within Malaysia, a combination of scholarly research reviews and consensus-driven methodologies, such as questionnaires and collaborative discussions, were utilized to guide the development process.

    SETTING: Multicenter study. Feedback obtains via face-to-face and online interaction.

    PARTICIPANTS: Cleft Clinicians, Cleft Lip and Palate Association of Malaysia members, hospitals, and government bodies personal.

    MAIN OUTCOME MEASURE: Participants provide their feedback and suggestions of each outcome measure.

    RESULTS: Through a deliberative process, agreement was established on a uniform set of outcome measures selected from an initial list of 108 potential outcomes. These agreed-upon measures were classified into 12 primary domains: demographic, cleft detail, pregnancy, general pediatric, otolaryngology/audiology, speech, surgical, orthodontics, pediatric dentistry, radiology, photographs, and study models.

    CONCLUSION: A specialized core outcome set has been successfully developed for patients with cleft lip and/or palate, aiming to facilitate its implementation within the Malaysian healthcare system.

  15. Harwansh RK, Hamid JA, Wal P, Deshmukh R, Patra PK, Gasmi A
    Curr Pharm Des, 2025 Feb 27.
    PMID: 40017252 DOI: 10.2174/0113816128334659241223113743
    OBJECTIVE OF THE STUDY: This review aims to critically analyze the scope for targeting drugs towards the treatment of improving outcomes in PDAC, focusing on DNA repair inhibitors, antiangiogenic therapy, inhibitors of the KRAS pathway, anti-stromal, and nanoparticle-based therapy.

    MATERIALS AND METHODS: A critical review of preclinical and clinical studies was conducted to summarize the therapeutic interventions that target specific mutations in PDAC, components of the tumor microenvironment, and drug delivery systems, especially nanotechnology, to enhance targeting and efficacy.

    RESULTS: Inhibitors and nanotechnology-based targeted therapies have reported promise in preclinical models: drug delivery is enhanced with the loss of PDAC resistance mechanisms. Formulations and combinations targeting KRAS as well as other pathways point toward improved drug delivery over 'orthodox' treatment approaches.

    CONCLUSION: This review concludes that although improvement in therapies for PDAC has incrementally been proven in recent literature, however, more research is expected to enhance these approaches so that they can be applied appropriately at the clinical stage. In future studies, it is expected to optimize treatment combinations, address mechanisms of resistance, and improve the delivery of drugs.

  16. Williamson F
    Br J Hist Sci, 2025 Feb 28.
    PMID: 40017310 DOI: 10.1017/S0007087424001523
    This article interrogates the positioning of British colonial meteorology in Malaysia and Singapore from the 1940s to 1960. This period spanned a global conflict and an internecine war, effecting profound sociopolitical changes from which neither Malaysia nor Singapore would emerge the same. The meteorological services were essential to Britain's armed conflicts, providing vital weather information to the army, navy and, especially, the air forces, as well as supporting the aviation and shipping industry often in difficult and dangerous circumstances. This article argues that British military policy in South East Asia and the specific concerns of the colonial government in Malaya directly commanded the meteorological agenda on the ground during this period, with a secondary but significant impact on tropical climate and weather research. It thus addresses the interplay of science, colonialism and military interest from the perspective of a region that has featured little in the history of science.
  17. Atiya N, Hasan MS, Wong CK, Abdullah A, Lau KS, Cheah EG, et al.
    Asia Pac J Public Health, 2025 Mar;37(2-3):265-271.
    PMID: 40017331 DOI: 10.1177/10105395251319923
    There are limited data on pulmonary tuberculosis (PTB) requiring intensive care unit (ICU) admission despite its high mortality rate. The objectives of our study were to determine the prevalence of culture-confirmed PTB requiring ICU admission and the trend in prevalence before and during the COVID-19 pandemic. A retrospective cohort study was conducted on all patients aged ≥18 years with culture-confirmed PTB requiring ICU admission in a Malaysian tertiary academic medical center from 2015 to 2021. The linear-by-linear association test was performed to determine if the trend in prevalence was significant. The overall prevalence of culture-confirmed PTB requiring ICU admission was 8.0% (139/1736). Between 2015 and 2021, the prevalence increased by 5.8% from 1.6% (5/312) to 7.4% (16/215). This study demonstrated an overall low but increasing trend in the prevalence of culture-confirmed PTB requiring ICU admission. Disruption in TB services during the COVID-19 pandemic may have impacted the prevalence trend.
    MeSH terms: Academic Medical Centers; Adult; Aged; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Retrospective Studies; Prevalence; Tertiary Care Centers
  18. Wah JNK
    Front Public Health, 2025;13:1530799.
    PMID: 40017541 DOI: 10.3389/fpubh.2025.1530799
    INTRODUCTION: The integration of Artificial Intelligence (AI) in healthcare, particularly through hybrid chatbots, is reshaping the industry by enhancing service delivery, patient engagement, and clinical outcomes. These chatbots combine AI with human input to provide intelligent, personalized interactions in areas like diagnostics, chronic disease management, and mental health support. However, gaps remain in trust, data security, system integration, and user experience, which hinder widespread adoption. Key challenges include the hesitancy of patients to trust AI due to concerns over data privacy and the accuracy of medical advice, as well as difficulties in integrating chatbots into existing healthcare infrastructures. The review aims to assess the effectiveness of hybrid AI chatbots in improving healthcare outcomes, reducing costs, and enhancing patient engagement, while identifying barriers to adoption such as cultural adaptability and trust issues. The novelty of the review lies in its comprehensive exploration of both technological advancements and the socio-emotional factors influencing chatbot acceptance.

    METHODS: The review follows a systematic methodology with four core components: eligibility criteria, review selection, data extraction, and data synthesis. Studies focused on AI applications and hybrid chatbots in healthcare, particularly in chronic disease management and mental health support, were included. Publications from 2022 to 2025 were prioritized, and peer-reviewed sources in English were considered. After screening 116 studies, 29 met the criteria for inclusion. Data was extracted using a structured template, capturing study objectives, methodologies, findings, and challenges. Thematic analysis was applied to identify four themes: AI applications, technical advancements, user adoption, and challenges/ethical concerns. Statistical and content analysis methods were employed to synthesize the data comprehensively, ensuring robustness in the findings.

    RESULTS: Hybrid chatbots in healthcare have shown significant benefits, such as reducing hospital readmissions by up to 25%, improving patient engagement by 30%, and cutting consultation wait times by 15%. They are widely used for chronic disease management, mental health support, and patient education, demonstrating their efficiency in both developed and developing countries.

    DISCUSSION: The review concludes that overcoming these barriers through infrastructure investment, training, and enhanced transparency is crucial for maximizing the potential of AI in healthcare. Future researchers should focus on long-term outcomes, addressing ethical considerations, and expanding cross-cultural adaptability. Limitations of the review include the narrow scope of some case studies and the absence of long-term data on AI's efficacy in diverse healthcare contexts. Further studies are needed to explore these challenges and the long-term impact of AI-driven healthcare solutions.

    MeSH terms: Artificial Intelligence*; Delivery of Health Care; Humans; Telemedicine*
  19. Subramaniam T, Mualif SA, Chan WH, Abd Halim KB
    Front Bioinform, 2025;5:1533983.
    PMID: 40017562 DOI: 10.3389/fbinf.2025.1533983
    Antibodies are naturally produced safeguarding proteins that the immune system generates to fight against invasive invaders. For centuries, they have been produced artificially and utilized to eradicate various infectious diseases. Given the ongoing threat posed by COVID-19 pandemics worldwide, antibodies have become one of the most promising treatments to prevent infection and save millions of lives. Currently, in silico techniques provide an innovative approach for developing antibodies, which significantly impacts the formulation of antibodies. These techniques develop antibodies with great specificity and potency against diseases such as SARS-CoV-2 by using computational tools and algorithms. Conventional methods for designing and developing antibodies are frequently costly and time-consuming. However, in silico approach offers a contemporary, effective, and economical paradigm for creating next-generation antibodies, especially in accordance with recent developments in bioinformatics. By utilizing multiple antibody databases and high-throughput approaches, a unique antibody construct can be designed in silico, facilitating accurate, reliable, and secure antibody development for human use. Compared to their traditionally developed equivalents, a large number of in silico-designed antibodies have advanced swiftly to clinical trials and became accessible sooner. This article helps researchers develop SARS-CoV-2 antibodies more quickly and affordably by giving them access to current information on computational approaches for antibody creation.
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