Browse publications by year: 2025

  1. Alhawarri MB, Al-Thiabat MG, Dubey A, Tufail A, Banisalman K, Al Jabal GA, et al.
    PLoS One, 2025;20(1):e0313094.
    PMID: 39775383 DOI: 10.1371/journal.pone.0313094
    Breast cancer remains a significant challenge in oncology, highlighting the need for alternative therapeutic strategies that target necroptosis to overcome resistance to conventional therapies. Recent investigations into natural compounds have identified 8,12-dimethoxysanguinarine (SG-A) from Eomecon chionantha as a potential necroptosis inducer. This study presents the first computational exploration of SG-A interactions with key necroptotic proteins-RIPK1, RIPK3, and MLKL-through molecular docking, molecular dynamics (MD), density functional theory (DFT), and molecular electrostatic potential (MEP) analyses. Molecular docking revealed that SG-A exhibited a stronger affinity for MLKL (-9.40 kcal/mol) compared to the co-crystallized ligand (-6.29 kcal/mol), while its affinity for RIPK1 (-6.37 kcal/mol) and RIPK3 (-7.01 kcal/mol) was lower. MD simulations further demonstrated the stability of SG-A within the MLKL site, with RMSD values stabilizing between 1.4 and 3.3 Å over 300 ns, indicating a consistent interaction pattern. RMSF analysis indicated the preservation of protein backbone flexibility, with average fluctuations under 1.7 Å. The radius of gyration (Rg) results indicated a consistent value of ~15.3 Å across systems, confirming the role of SG-A in maintaining protein integrity. Notably, SG-A maintains two critical H-bonds within the active site of MLKL, reinforcing the stability of the interaction. Principal component analysis (PCA) indicated a significant reduction in MLKL's conformational space upon SG-A binding, implying enhanced stabilization. Dynamic cross-correlation map (DCCM) analysis further revealed that SG-A induced highly correlated motions, reducing internal fluctuations within MLKL compared to the co-crystallized ligand. MM-PBSA revealed the enhanced binding efficacy of SG-A, with a significant binding free energy of -31.03 ± 0.16 kcal/mol against MLKL, surpassing that of the control (23.96 ± 0.11 kcal/mol). In addition, the individual residue contribution analysis highlighted key interactions, with ARG149 showing a significant contribution (-176.24 kcal/mol) in the MLKL-SG-A complex. DFT and MEP studies corroborated these findings, revealing that the electronic structure of SG-A is conducive to stable binding interactions, characterized by a narrow band gap (~0.16 units) and distinct electrostatic potential favourable for necroptosis induction. In conclusion, SG-A has emerged as a compelling inducer of necroptosis for breast cancer therapy, warranting further experimental validation to fully realize its therapeutic potential.
    MeSH terms: Breast Neoplasms/drug therapy; Breast Neoplasms/metabolism; Breast Neoplasms/pathology; Female; Humans; Isoquinolines/metabolism; Isoquinolines/pharmacology; Isoquinolines/chemistry; Protein Binding; Protein Kinases/metabolism; Protein Kinases/chemistry; Receptor-Interacting Protein Serine-Threonine Kinases/metabolism; Receptor-Interacting Protein Serine-Threonine Kinases/chemistry; Static Electricity*; Molecular Dynamics Simulation*; MCF-7 Cells; Molecular Docking Simulation*
  2. Lourijsen E, Avdeeva K, Gan KL, Fokkens W
    Cochrane Database Syst Rev, 2025 Jan 07;1:CD013476.
    PMID: 39775459 DOI: 10.1002/14651858.CD013476.pub2
    BACKGROUND: NSAID-exacerbated respiratory disease (N-ERD) is a hypersensitivity to non-steroidal anti-inflammatory drugs (NSAIDs), such as aspirin or ibuprofen, accompanied by chronic rhinosinusitis (with or without nasal polyps) or asthma. The prevalence of hypersensitivity to NSAIDs is estimated to be 2%. The first line of treatment is the avoidance of NSAIDs. Another treatment option is aspirin treatment after desensitisation (ATAD). Desensitisation can be induced by repeated administration of aspirin at fixed time intervals. The clinical benefit of aspirin might occur through inhibition of interleukin 4 and a reduction in prostaglandin D2. This therapy can be useful for people who have progressive airway disease and are in great need of medical intervention (mostly systemic corticosteroids) or surgery. An up-to-date Cochrane review is vital to investigate the effects of this therapy.

    OBJECTIVES: To assess the effectiveness of oral or intranasal aspirin desensitisation, as monotherapy or as adjunctive therapy, in adults with NSAID-exacerbated respiratory disease.

    SEARCH METHODS: The Cochrane Ear Nose and Throat (ENT) Information Specialist searched the Cochrane ENT and Airways Trials Registers; Central Register of Controlled Trials (CENTRAL); Ovid MEDLINE; Ovid Embase; Web of Science; ClinicalTrials.gov; International Clinical Trials Registry Platform and additional sources for published and unpublished trials. The date of the search was 10 February 2023.

    SELECTION CRITERIA: Randomised controlled trials that compared ATAD with placebo were eligible. We included studies of adults with NSAID-exacerbated respiratory disease (i.e. intolerance to NSAID established, e.g. by aspirin challenge test), with chronic rhinosinusitis or asthma, or both. Participants had to be followed up for at least three months.

    DATA COLLECTION AND ANALYSIS: We used standard Cochrane methods. The primary outcomes were health-related quality of life, asthma control, and significant serious and non-serious adverse events. The secondary outcomes were changes in airway assessments, nasal endoscopy score, medication use, symptom scores, and chronic rhinosinusitis and asthma exacerbations (description of exacerbation for which systemic corticosteroid or sinus surgery was needed). We used the GRADE approach to rate the certainty of the evidence.

    MAIN RESULTS: We included five studies with a total of 211 participants (146 analysed). All studies compared oral ATAD at different dosages with placebo and were performed in tertiary care centres. All participants had a diagnosis of chronic rhinosinusitis with nasal polyps. In four studies, participants also had a confirmed diagnosis of asthma and two studies reported that participants had previous surgery for nasal polyps. Outcomes were analysed at six and 36 months follow-up. However, only one study reported data for 36 months follow-up. All but one study reported source of funding. Mid-term follow-up (six months, ATAD versus placebo) ATAD may improve health-related quality of life, assessed with Sino-Nasal Outcome Test (SNOT) scores (mean difference (MD) -0.54, 95% confidence interval (CI) -0.76 to -0.31; 3 studies, 85 participants; minimum clinically important difference (MCID) 9.0 points for total score; low-certainty evidence). In this analysis, SNOT-22 scores were divided by 22 and SNOT-20 scores were divided by 20. The mean reduction (11.9 points) in SNOT score (based on SNOT-22) is larger than the MCID. It is uncertain if asthma control may be improved after ATAD. Asthma control was measured using the Asthma Control Test (ACT) in one study and the Asthma Control Questionnaire (ACQ) in another study, so data were not pooled. The MD on the ACQ was -2.00 (total score 0 to 6) (95% CI -4.30 to 0.30; 1 study, 15 participants; MCID 0.5 points; very low-certainty evidence). The MD on the ACT was 5.90 (total score 5 to 25) (95% CI 2.93 to 8.87; 1 study, 30 participants; MCID 3 points; very low-certainty evidence). All but one study reported on adverse events. Seven participants in the active treatment group developed a gastrointestinal disorder and dropped out (129 participants, very low-certainty evidence). We are uncertain of the effect of ATAD on nasal airflow, measured by peak nasal inspiratory flow scores (MD 32.90 L/min, 95% CI -12.44 to 78.24; 1 study, 15 participants; very low-certainty evidence). It is uncertain if the dosage of intranasal or inhaled corticosteroids may be reduced with ATAD (inhaled corticosteroids: -1197.60 µg, 95% CI -1744.93 to -650.27; intranasal corticosteroids: -120.50 µg, 95% CI -206.49 to -34.51; 1 study; 15 participants; very low-certainty evidence). Symptom scores may not differ between ATAD and placebo, but the evidence is very uncertain (sneezing: MD -0.70, 95% CI -1.45 to 0.05; smell: MD -2.20, 95% CI -4.74 to 0.34; nasal blockage: MD -0.90, 95% CI -1.90 to 0.10; 1 study, very low-certainty evidence). No study assessed nasal endoscopy at this time point. Long-term follow-up (36 months, ATAD versus placebo) ATAD may improve quality of life, as measured with the Rhinosinusitis Disability Index (RSDI) score (MD-18.10, 95% CI -32.82 to -3.38; 1 study; 31 participants; low-certainty evidence). ATAD may result in little to no difference in the size of nasal polyps (MD -1.20, 95% CI -2.72 to 0.32; 1 study, 31 participants; very low-certainty evidence). No adverse events were reported in either group over the total study period of 36 months (1 study; 31 participants; very low-certainty evidence). Data on peak nasal inspiratory flow, changes in dosage of inhalation or intranasal corticosteroids and symptom scores were not reported at this time point.

    AUTHORS' CONCLUSIONS: Aspirin treatment after desensitisation may improve health-related quality of life for people with N-ERD with a follow-up of six months. With respect to asthma control, adverse events, peak nasal inspiratory flow score, nasal endoscopy scores, changes in dosage of inhaled or intranasal corticosteroids, nasal and bronchial symptom scores, exacerbations or worsening of asthma and chronic rhinosinusitis (including the need for surgery), the evidence is inconclusive for the short-term and long-term. We did not find data on peak expiratory flow. It is difficult to interpret the results adequately, due to the potential influence of the use of any co-medications for chronic rhinosinusitis or asthma. Future research should emphasise longer duration of follow-up, report baseline disease characteristics and report on compliance and exacerbations for which additional medication or surgery is warranted.

    MeSH terms: Administration, Intranasal; Administration, Oral; Adult; Humans; Randomized Controlled Trials as Topic
  3. Yang R, Juma NS, Zhao Y, Zheng B, Xu Y, Gao Y, et al.
    Compr Rev Food Sci Food Saf, 2025 Jan;24(1):e70067.
    PMID: 39776157 DOI: 10.1111/1541-4337.70067
    Gelation and gel properties are crucial to surimi-based seafood products, and many factors significantly influence surimi gel quality. Although physical and chemical modifications can improve surimi gel performance, challenges such as high cost, difficulties in industrialization and environmental pollution pose significant barriers to their practicality. Natural additives offer a promising alternative by reinforcing and improving the characteristics of surimi gel through mechanisms such as protein conformational transformation, protein denaturation, and altered chemical forces. By incorporating different substances into surimi gel, it is possible to tune the interaction between the additives and the myofibrillar proteins, thus enhancing the gelation process and achieving the desired textural profiles. This review comprehensively explored the factors influencing the surimi gelation chemistry, with a focus on how the natural additives such as proteins, lipids, polysaccharides, salts, enzymes, and extracts impact the surimi gel properties. It elucidated the reinforcing mechanisms of these additives and proposed a general interaction model between natural substance and myofibrillar proteins. Furthermore, this review well established the interrelation between the performance and mechanism of enhancement effects of typical natural substances on surimi gels and provided new insights on tuning surimi gelation and gel properties by adding natural additives with specific physicochemical properties, thus facilitating the production of high-quality surimi products with satisfactory gel characteristics in food industry.
    MeSH terms: Animals; Fish Products/analysis; Food Additives/chemistry; Food Handling/methods; Food, Fortified
  4. Wah JNK
    J Robot Surg, 2025 Jan 07;19(1):47.
    PMID: 39776281 DOI: 10.1007/s11701-024-02205-0
    Artificial intelligence and robotics are revolutionizing surgical practices by enhancing precision, efficiency, and patient outcomes. With global healthcare systems increasingly adopting AI-driven technologies, the integration of robotics in surgery addresses critical challenges such as surgical accuracy, minimally invasive techniques, and healthcare accessibility. However, disparities in access and ethical concerns regarding automation persist globally, necessitating a balanced discourse on these advancements. The primary objective of the review is to explore the intersection of AI and robotic technologies in surgical practices, highlighting key innovations, their impact on precision medicine, and future trajectories. The novelty of the review lies in synthesizing findings across diverse surgical specialties, including urology, plastic surgery, ophthalmology, and pediatric surgery, to provide a comprehensive understanding of AI's transformative role. The systematic review evaluates the integration of AI and robotics in surgery, synthesizing studies published in 2024. It follows PRISMA guidelines for study selection, data extraction, and synthesis. The review assesses surgical specialties, AI technologies, and outcomes like accuracy, recovery, and complications, ensuring methodological rigor and transparency. The findings indicate substantial advancements in AI-driven surgical systems, improving decision-making, reducing surgical errors, and facilitating personalized treatment strategies. Notable breakthroughs include AI-enhanced imaging, real-time data analysis, and automated robotic instruments, which collectively improve procedural efficiency and patient safety. The discussion emphasizes the critical implications of these innovations, particularly in advancing minimally invasive techniques and expanding surgical capabilities in complex procedures. However, challenges such as cost, ethical considerations, and the need for rigorous training protocols are highlighted as barriers to widespread adoption. The review underscores the implications for policymakers, healthcare institutions, and technology developers to address these barriers and promote equitable access to AI-driven surgical solutions. Limitations include the reliance on recent studies, necessitating long-term evaluations of clinical outcomes. Future research should focus on integrating AI with emerging technologies like augmented reality, fostering interdisciplinary collaboration, and addressing socio-ethical dimensions to fully realize the potential of AI in surgical advancements.
    MeSH terms: Artificial Intelligence*; Humans; Inventions
  5. Akinshipo AW, Sivaramakrishnan G, Enwuchola J, Effiom O, Adeoye J, Ramanathan A, et al.
    Head Neck Pathol, 2025 Jan 07;19(1):2.
    PMID: 39776309 DOI: 10.1007/s12105-024-01739-x
    OBJECTIVE: To analyze the frequency, clinical, histopathological, and radiological characteristics of ameloblastoma in Nigeria over the course of two decades.

    STUDY DESIGN: A retrospective analysis was conducted on 371 cases at a Nigerian university hospital between 2000 and 2023. Age, gender, site, histological variants, tumor size and duration were analyzed. Statistical analyses included the Shapiro-Wilk test, Mann-Whitney U test, Chi-square test, and Spearman rank correlation analysis.

    RESULTS: The median patient age was 30 years (mean age 32.2), with a male-to-female ratio of 1.12:1. 54.7% of cases occurred in young adults (age range 20-39 years). Among the lesions, 11.3% were in the maxilla and 88.7% in the mandible. Patients with mandibular lesions had a median age of 29 years, while those with maxillary lesions had a statistically significantly higher median age of 37.5 years p-value = 0.001. Median tumor size was 36 cm2 for the mandible and 24 cm2 for the maxilla (significant p-value of 0.002). There was no correlation between tumor size, age, or gender. However, there was a significant correlation between tumor size and the duration of the condition.

    CONCLUSION: The study concludes that ameloblastoma is more frequent among younger individuals in Nigeria and often presents with larger tumor sizes, emphasizing the need for early detection and intervention.

    MeSH terms: Adolescent; Adult; Aged; Child; Child, Preschool; Female; Humans; Male; Middle Aged; Nigeria; Retrospective Studies; Young Adult
  6. Jairoun AA, Ping CC, Ibrahim B, Al Jawamis DF, Al Jaberi AK, Dawoud T, et al.
    J Pharm Policy Pract, 2025;18(1):2414293.
    PMID: 39776464 DOI: 10.1080/20523211.2024.2414293
    BACKGROUND: Chronic Kidney Disease (CKD) represents a significant worldwide health challenge, with far-reaching implications for both patients and healthcare systems. This study aimed to identify the incidence of CKD at stages 3-5, analyzed the impact of statin and other antihyperglycemic interventions, on the CKD progression in individuals with T2DM.

    METHODS: This was a single-center retrospective cohort study based on data derived from electronic medical records (EMR) of UAE populations with diabetes mellitus, registered at outpatient clinics at Tawam Hospital in Al Ain, UAE, between January 2011 and December 2021. T2DM patients aged ≥ 18 years who had serum HbA1c level ≥ 6.5% and using one of the statin therapies were inclusion criteria. Patients with T1DM, who had undergone permanent renal replacement therapy, with under 1 year of follow-up and missing or incomplete data were excluded from the study. The collected data encompassed socio-demographics, detailed medical history, anthropometric measurements, laboratory analyses, clinical parameters, disease characteristics, and medications.

    RESULTS: Our study included a cohort of 1,003 individuals. We observed 388 subjects developed CKD stages 3-5 across an average monitoring duration of 11.7 years. This resulted in a cumulative incidence of 38.7%, translating to an incidence rate of 38 cases per 1000 person-years. There was a statistically significant difference in the cumulative incidence of CKD stages 3 ± 5 according to statin therapy (P = 0.047). High intensity statin users are more likely to develop a CKD stage 3-5 compared to low/moderate intensity users and to no statin users respectively (44.3% vs 37.9%), (44.3% vs 30.9%). Conversely, the use of Biguanides was associated with a decreased probability of CKD progression (37.9% vs. 52.8%; P = 0.001), whereas Insulin users demonstrated a heightened risk (54.2% vs. 34.1%; P 

  7. Ramadhan M, AlMehandi A, Al-Naseem A, Hayat J, Almarzouq A
    Arab J Urol, 2025;23(1):84-94.
    PMID: 39776556 DOI: 10.1080/20905998.2024.2395202
    PURPOSE: To compare the outcomes of using prophylactic direct oral anti-coagulants (DOAC) and low-molecular-weight heparin (LMWH) after major urologic surgery.

    MATERIALS AND METHODS: Systematic literature searches of MEDLINE, Embase, Web of Science, and Cochrane CENTRAL were performed up to 9 November 2023, and protocols were registered on PROSPERO (CRD42024494424). The primary outcomes were post-operative incidence of VTE and bleeding. The secondary outcomes included re-admissions and transfusions needed, post-operative complications and exploring the radical cystectomy sub-group. Outcomes were reported in 30 and 90 days where feasible with sub-group analysis.

    RESULTS: Searches yielded four studies that included 856 patients and the outcomes were reported within 30 and 90 days, with sub-analysis performed for each time-interval. We found no statistically significant differences between DOAC and LWMH within neither primary nor secondary outcomes; VTE events (RR 0.36; p = 0.06); bleeding events (RR 0.64; p = 0.45); re-admissions (RR 1.14; p = 0.39); transfusions (RR 0.42; p = 0.05) within 0-90 days and post-operative complications within 30 days (RR 0.76; p = 0.17). Similar results were found when exploring radical cystectomy sub-group: VTE risk (RR 0.42, p = 0.15), bleeding risk (RR 1.09; p = 0.90), and re-admissions to hospital (RR 1.18, p = 0.35). Limitations include small sample size, and difficult generalization to all urological surgery as most of the analyzed cohort underwent radical cystectomy.

    CONCLUSION: DOACs may be a safe and possibly cost-effective alternative to LMWH as post-operative thromboprophylaxis. However, these findings should be interpreted with caution due to limitations; therefore, more randomized studies are needed to ascertain our findings.

  8. Miah MH, Rahman MB, Nur-E-Alam M, Islam MA, Shahinuzzaman M, Rahman MR, et al.
    RSC Adv, 2025 Jan 02;15(1):628-654.
    PMID: 39777157 DOI: 10.1039/d4ra07942f
    Perovskite materials have garnered significant attention within a very short period of time by achieving competitive efficiency. In addition, this material demonstrated intriguing optoelectronic properties and versatile applications. Although they have confirmed amazing efficiency in solar cells at the laboratory scale, mass commercial manufacturing of perovskite solar cells (PSCs) is still a problem due to their poor longevity. Researchers have identified several intrinsic and extrinsic factors contributing to the instability of perovskite compounds and PSCs, and various approaches are being used to increase material quality and stability in order to extend the lifespan of PSCs. Despite these challenges, the potential of perovskite materials in revolutionizing solar energy remains a central point of scientific investigation and development. In this review, a comprehensive analysis is provided to discern the intrinsic and extrinsic factors contributing to the degradation of PSCs which certainly helps us to understand the underlying degradation mechanisms. In addition, we discussed some novel approaches that have already been adopted to augment the stability of the devices.
  9. Hu D, Li Y, Zhang H, Wang LL, Liu WW, Yang X, et al.
    World J Gastroenterol, 2025 Jan 07;31(1):100357.
    PMID: 39777240 DOI: 10.3748/wjg.v31.i1.100357
    BACKGROUND: Return to work (RTW) serves as an indication for young and middle-aged colorectal cancer (CRC) survivors to resume their normal social lives. However, these survivors encounter significant challenges during their RTW process. Hence, scientific research is necessary to explore the barriers and facilitating factors of returning to work for young and middle-aged CRC survivors.

    AIM: To examine the current RTW status among young and middle-aged CRC survivors and to analyze the impact of RTW self-efficacy (RTW-SE), fear of progression (FoP), eHealth literacy (eHL), family resilience (FR), and financial toxicity (FT) on their RTW outcomes.

    METHODS: A cross-sectional investigation was adopted in this study. From September 2022 to February 2023, a total of 209 participants were recruited through a convenience sampling method from the gastrointestinal surgery department of a class A tertiary hospital in Chongqing. The investigation utilized a general information questionnaire alongside scales assessing RTW-SE, FoP, eHL, FR, and FT. To analyze the factors that influence RTW outcomes among young and middle-aged CRC survivors, Cox regression modeling and Kaplan-Meier survival analysis were used.

    RESULTS: A total of 43.54% of the participants successfully returned to work, with an average RTW time of 100 days. Cox regression univariate analysis revealed that RTW-SE, FoP, eHL, FR, and FT were significantly different between the non-RTW and RTW groups (P < 0.05). Furthermore, Cox regression multivariate analysis identified per capita family monthly income, job type, RTW-SE, and FR as independent influencing factors for RTW (P < 0.05).

    CONCLUSION: The RTW rate requires further improvement. Elevated levels of RTW-SE and FR were found to significantly increase RTW among young and middle-aged CRC survivors. Health professionals should focus on modifiable factors, such as RTW-SE and FR, to design targeted RTW support programs, thereby facilitating their timely reintegration into mainstream society.

    MeSH terms: Adult; China/epidemiology; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Surveys and Questionnaires; Self Efficacy*; Resilience, Psychological*; Young Adult
  10. Subramaniam P, Thillainathan P, Angwin A, Sharma S
    Adv Clin Exp Med, 2025 Jan 08.
    PMID: 39778016 DOI: 10.17219/acem/194410
    BACKGROUND: Dementia, a rapidly growing cognitive disorder, has a profound impact on the lives of individuals and their caregivers across the globe. Digital life storybooks have emerged as a promising non-pharmacological intervention to improve the wellbeing of people living with dementia (PLWD).

    OBJECTIVES: This study aims to investigate the feasibility of developing and applying a digital life storybook for PLWD using telehealth, while evaluating its impact on communication skills, quality of life (QoL) and satisfaction levels.

    MATERIAL AND METHODS: A mixed-method study design will be employed, involving pairs of PLWD and their primary caregivers (dyads) recruited from a teaching hospital and a non-profit organization in Malaysia. The intervention involves the creation and use of a digital life storybook facilitated remotely via telehealth channels. Data will be collected at 6 points in time: prior to the commencement of development, prior to the submission of an application, on a biweekly basis, and at the conclusion of the assessment period. Quantitative measures will include the Holden Communication Scale, Quality of Life-Alzheimer's Disease Scale (QoL-AD) and Quality of the Caregiving Relationship (QCPR) questionnaire. Qualitative data will be gathered through validated open-ended questions.

    RESULTS: Implications of the study include facilitating future research, contributing to person-centered care practices, and providing caregivers with tools to better understand and connect with PLWD. The findings will contribute to the understanding of the mechanisms through which digital life storybooks can benefit PLWD and their caregivers.

    CONCLUSION: The successful implementation of this protocol could have significant implications for dementia care in both formal and informal settings, and could ultimately improve the lives of those affected by dementia.

  11. Zhi-Xiong C
    Prog Biophys Mol Biol, 2025 Jan 06.
    PMID: 39778630 DOI: 10.1016/j.pbiomolbio.2025.01.002
    Ovarian cancer is one of the most prevalent gynaecological malignancies. The rapid development of single-cell RNA sequencing (scRNA-seq) has allowed scientists to use this technique to study ovarian cancer development, heterogeneity, and tumour environment. Although multiple original research articles have reported the use of scRNA-seq in understanding ovarian cancer and how therapy resistance occurs, there is a lack of a comprehensive review that could summarize the findings from multiple studies. Therefore, this review aimed to fill this gap by comparing and summarizing the results from different studies that have used scRNA-seq in understanding ovarian cancer development, heterogeneity, tumour microenvironment, and treatment resistance. This review will begin with an overview of scRNA-seq workflow, followed by a discussion of various applications of scRNA-seq in studying ovarian cancer. Next, the limitations and future directions of scRNA-seq in ovarian cancer research will be presented.
  12. Sadikan MZ, Lambuk L, Reshidan N, Ahmad Hairi H, Abd Ghapor AA, Mohamud R, et al.
    J Ocul Pharmacol Ther, 2025 Jan 08.
    PMID: 39778903 DOI: 10.1089/jop.2024.0125
    Vitamin E is renowned for its potent antioxidant properties, crucial for shielding cells against oxidative stress and damage. Deficiency in this vitamin can lead to various health issues, including neurodegenerative diseases, due to its pivotal role in preserving cell membrane integrity and combating cellular oxidative damage. While its importance for overall health, including neurodegeneration, is acknowledged, the specific correlation between vitamin E deficiency and distinct ocular neurodegenerative disorders need to be further explored. This review delves into the molecular mechanisms of vitamin E in ocular neurodegenerative disorders; diabetic retinopathy, age-related macular degeneration, glaucoma, and cataracts, and emphasising the therapeutic implications drawn from existing evidence. Relationship between vitamin E and ocular neurodegenerative disorders is widely researched on, with its primary protective mechanisms attributed to its antioxidant and anti-inflammatory properties. However, studies on the supplementation of vitamin E among human subjects present mixed results, suggesting its complexities and variability depending on factors such as the specific disorder, disease stage, genetic differences, and form of vitamin E utilized. In conclusion, while vitamin E holds promise in mitigating ocular neurodegeneration through its antioxidant and anti-inflammatory properties, its supplementation's efficacy remains nuanced and context dependent. More research works are essential to elucidate its precise role and therapeutic potential in combating various ocular neurodegenerative disorders.
  13. Othman MZ, Mohd Nasir MH, Wan Ahmad WAN, Abdullah JM, Che Has AT
    Exp Anim, 2025 Jan 09.
    PMID: 39779249 DOI: 10.1538/expanim.24-0120
    Status epilepticus is linked to cognitive decline due to damage to the hippocampus, a key structure involved in cognition. The hippocampus's high vulnerability to epilepsy-related damage is the main reason for this impairment. Convulsive seizures, such as those observed in status epilepticus, can cause various hippocampal pathologies, including inflammation, abnormal neurogenesis, and neuronal death. Interestingly, substantial evidence points to the therapeutic potential of the sedative/hypnotic agent zolpidem for neurorehabilitation in brain injury patients, following the unexpected discovery of its paradoxical awakening effect. In this study, we successfully established an ideal lithium-pilocarpine rat model of status epilepticus, which displayed significant deficits in hippocampal-dependent learning and memory. The Morris water maze test was used to assess zolpidem's potential to improve learning and memory, as well as its impact on anxiety-like behavior and motor function. Immunohistochemical staining and fluorescence analysis were used to examine the effect of zolpidem on KCC2 and NKCC1 protein expression in the hippocampal CA1 and CA3. Our findings showed that zolpidem did not improve learning and memory in status epilepticus rats. Additionally, its sedative/hypnotic effects were not apparent in the status epilepticus condition. However, immunohistochemical results revealed that zolpidem significantly restored altered NKCC1 levels in the CA1 and CA3 to levels similar to those seen in normal rats. These findings suggest that zolpidem may contribute to molecular restoration, particularly through its impact on NKCC1 protein expression in the hippocampus, which is crucial for proper inhibitory neurotransmission in the brain.
  14. Phoa PKA, Wee LH, Wong YH, Siau CS, Chan CMH, Dain C, et al.
    Tob Control, 2025 Jan 08.
    PMID: 39779329 DOI: 10.1136/tc-2024-058874
    In Malaysia, tobacco smoking continues to be one of the leading public health concerns; hence, the tobacco control community aims to see a generation free of tobacco use by 2040. Drafted and presented to the parliament, the Malaysian Control of Smoking Products for Public Health Bill 2022 highlighted the Generational Endgame (GEG) policy, which forbids the use and sale of tobacco products and smoking substances to individuals born on or after 1 January 2007. Stakeholders, including government and non-governmental organisations, policymakers, healthcare professionals, tobacco industry representatives and retailers, have expressed differing opinions indicating non-support of the policy. The Attorney General Chamber deemed the policy as 'unconstitutional' for discriminating against those within the implementation age range, which prompted its omission from the revised Control of Smoking Products for Public Health 2023 Bill. This paper discusses the obstacles and possible implications of the GEG policy implementation in Malaysia and details its implementation in other countries. This paper also proposes several recommendations for future directions in tackling the obstacles mentioned more effectively.
  15. Che Rahim NF, Hussin Y, Aziz MNM, Masarudin MJ, Abd Gani S, Akhtar MN, et al.
    PMID: 39779570 DOI: 10.2174/0118715206336788241029050155
    BACKGROUND: Colorectal cancer (CRC) is the second-leading cause of cancer-related deaths. Curcumin has been reported to have suppressive effects in CRC and to address the physiological limitations of curcumin, a chemically synthesized curcuminoid analog, known as (2E,6E)-2,6-Bis (2,3-Dimethoxy benzylidine) cyclohexanone (DMCH), was developed and the anti-metastatic and anti-angiogenic properties of DMCH in colorectal cell line, SW620 were examined.

    METHODS: The anti-metastatic effects of DMCH were examined in the SW620 cell line by scratch assay, migration, and invasion assay, while for anti-angiogenesis properties of the cells, the mouse aortic ring assay and Human Umbilical Vein Endothelial Cells (HUVEC) assay were conducted. The mechanism of action was determined by microarray-based gene expression and protein analyses.

    RESULTS: The wound healing assay demonstrated that wound closure was decreased from 63.63 ± 1.44% at IC25 treatment to 4.54 ± 0.62% at IC50 treatment. Significant (p<0.05) reductions in the percentage of migrated and invaded cells were also observed in SW620, with values of 36.39 ± 3.86% and 44.81 ± 3.54%, respectively. Mouse aortic ring assays demonstrated a significant reduction in the formation of tubes and microvessels. Microarray and protein profiler results revealed that DMCH treatment has modulated several metastases, angiogenesisrelated transcripts, and proteins like Epidermal Growth Factor Receptor (EGFR), TIMP-1 (TIMP Metallopeptidase Inhibitor 1) and Vascular Endothelial Growth Factor (VEGF).

    CONCLUSION: DMCH could be a potential anti-cancer agent due to its capability to impede metastasis and angiogenesis activities of the SW620 colorectal cancer cell line in vitro via regulating genes and protein in metastases and angiogenesis-related signalling pathways.

  16. Cheung DH, Waratworawan W, Kongjareon Y, Jonas KJ, Lim SH, Reeves AN, et al.
    AIDS Behav, 2025 Feb;29(2):420-434.
    PMID: 39779629 DOI: 10.1007/s10461-024-04516-7
    This study investigated the clustering of psychosocial adversities and their synergistic effect with depression on suicidality in a 12-month prospective cohort (N = 214) of YMSM living with HIV in Bangkok, Thailand. Latent class analysis identified subgroups with distinct combinations of adversities, including bullying, intimate partner violence, substance use, HIV stigma, low social support, histories of sex work, and below-income status. Significant interactive synergism were found as hypothesized, supporting a syndemic effect with qualitatively increasing levels of adversities on suicidality (score range: 3-17) over the 12 months. The interaction between moderate adversity clustering and depression (βow = 2.50, 95% CI: 1.12-3.88) and high adversity clustering and depression (βow = 3.61, 95% CI: 1.12-6.09) indicated that the impact of depression on suicidality was modified by pre-existing adversities. The findings suggest that, while a multi-component intervention addressing psychosocial problems is ideal, effective depression treatment alone could significantly reduce suicidality among YMSM living with HIV.
    MeSH terms: Intimate Partner Violence/psychology; Intimate Partner Violence/statistics & numerical data; Adult; Female; Humans; Male; Prospective Studies; Prostitution/psychology; Risk Factors; Social Support*; Suicide/psychology; Suicide/statistics & numerical data; Thailand/epidemiology; Substance-Related Disorders/epidemiology; Substance-Related Disorders/psychology; Young Adult; Social Stigma*; Suicidal Ideation
  17. Yin J, Ibrahim S, Mohd NNA, Zhong C, Mao X
    Sci Rep, 2025 Jan 08;15(1):1267.
    PMID: 39779724 DOI: 10.1038/s41598-025-85168-y
    The growth in carbon emissions poses a severe challenge to global sustainable development, making it imperative to explore the impacts of economic restructuring and technological progress on Carbon Emission Performance (CEP). However, existing studies often lack an integrated analysis of economic restructuring and technological progress, while giving limited attention to the indirect role of Environmental Regulation (ER). This study constructs a multidimensional theoretical framework, breaking down economic restructuring into four dimensions-industrial structure, factor input, ownership, and new-type urbanization (NTU), and refining technological progress into technological innovation and energy efficiency. It uncovers the complex interplay among CEP, economic restructuring, technological progress, and ER. The findings reveal critical insights: (1) industrial structure suppresses CEP, whereas adjustments in factor input and ownership structure significantly enhance it; (2) the relationship between NTU and CEP exhibits a non-linear pattern; (3) compared to the technological innovation, energy efficiency provide a more substantial boost to CEP; and (4) ER positively moderates the impacts of factor input, ownership structure, and NTU. Finally, the study proposes recommendations for holistic economic restructuring and diversified ERs.
  18. Khan MA, Zhang B, Ahmad M, Niekurzak M, Khan MS, Sabri Sabri MM, et al.
    Sci Rep, 2025 Jan 09;15(1):1385.
    PMID: 39779787 DOI: 10.1038/s41598-025-85363-x
    Addressing environmental challenges such as pollution and resource depletion requires innovative industrial and municipal waste management approaches. Cement production, a significant contributor to greenhouse gas emissions, highlights the need for eco-friendly building materials to combat global warming and promote sustainability. This study evaluates the simultaneous use of Sugarcane Bagasse Ash (SCBA) and Stone Dust (SD) as partial replacements by volume for cement and sand, respectively, at varying ratios in eco-strength concrete mixes designed for 28 MPa (ES-28) and 34 MPa (ES-34), emphasizing their economic and environmental benefits. The influence of SCBA and SD on workability, mechanical properties, and durability were experimentally investigated. Results reveal that for ES-28, with 9% SCBA and 50% SD, compressive and tensile strengths were nearly equal to the control mix, while flexural strength improved by 6.86%. For ES-34, with 9% SCBA and 50% SD, compressive strength was enhanced by 10.16%, tensile strength by 11.68%, and flexural strength by 5.22%, compared to the control mix. This improvement is attributed to pozzolanic reactions, enhanced particle packing, and optimal curing conditions. However, water absorption increased significantly, with ES-28 showing a 31.61% rise and ES-34 a 22.32% rise when SCBA was 9% and SD was 50%. These results highlight the trade-offs between mechanical performance and durability. The optimized mix, derived from response surface analysis, demonstrates significant potential as a sustainable alternative to conventional concrete, aligning with environmental and structural performance objectives.
  19. Sayer CA, Fernando E, Jimenez RR, Macfarlane NBW, Rapacciuolo G, Böhm M, et al.
    Nature, 2025 Feb;638(8049):138-145.
    PMID: 39779863 DOI: 10.1038/s41586-024-08375-z
    Freshwater ecosystems are highly biodiverse1 and important for livelihoods and economic development2, but are under substantial stress3. To date, comprehensive global assessments of extinction risk have not included any speciose groups primarily living in freshwaters. Consequently, data from predominantly terrestrial tetrapods4,5 are used to guide environmental policy6 and conservation prioritization7, whereas recent proposals for target setting in freshwaters use abiotic factors8-13. However, there is evidence14-17 that such data are insufficient to represent the needs of freshwater species and achieve biodiversity goals18,19. Here we present the results of a multi-taxon global freshwater fauna assessment for The IUCN Red List of Threatened Species covering 23,496 decapod crustaceans, fishes and odonates, finding that one-quarter are threatened with extinction. Prevalent threats include pollution, dams and water extraction, agriculture and invasive species, with overharvesting also driving extinctions. We also examined the degree of surrogacy of both threatened tetrapods and freshwater abiotic factors (water stress and nitrogen) for threatened freshwater species. Threatened tetrapods are good surrogates when prioritizing sites to maximize rarity-weighted richness, but poorer when prioritizing based on the most range-restricted species. However, they are much better surrogates than abiotic factors, which perform worse than random. Thus, although global priority regions identified for tetrapod conservation are broadly reflective of those for freshwater faunas, given differences in key threats and habitats, meeting the needs of tetrapods cannot be assumed sufficient to conserve freshwater species at local scales.
    MeSH terms: Agriculture; Animals; Conservation of Natural Resources; Crustacea; Fishes*; Fresh Water*; Water Pollution; Biodiversity*; Extinction, Biological*; Introduced Species/statistics & numerical data; Aquatic Organisms
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