Browse publications by year: 2025

  1. Seman-Kamarulzaman AF, Muhammad NAN, Othman NW, Jalinas J, Hassan NI, Rahmat NL, et al.
    Arch Insect Biochem Physiol, 2025 Feb;118(2):e70039.
    PMID: 39930668 DOI: 10.1002/arch.70039
    Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Dryophthoridae), commonly known as the red palm weevil (RPW), is a globally significant pest that threatens economically important palm trees. Its cryptic infestation behavior leads to irreversible damage and eventual host plant death. Current control methods using broad-spectrum insecticides are largely ineffective due to resistance development and their adverse effects on nontarget organisms, necessitating novel strategies. This study integrates proteomics and transcriptomics data to explore the molecular landscape of RPW and identify pathways for targeted pest management. A total of 16,954 transcripts and 983 proteins were identified across three developmental stages (larvae, male adults, and female adults), with a notable decline in protein numbers from larvae to adult. Differential expression analysis revealed 7540 proteins varying significantly between developmental stages. Through subtractive analysis, 218 proteins meeting stringent inclusion and exclusion criteria were identified. These proteins underwent pathway enrichment analysis, mapping to 39 enriched pathways (p-value and an FDR of
    MeSH terms: Animals; Beetles/genetics; Beetles/growth & development; Beetles/metabolism; Female; Insect Control/methods; Male; Pest Control/methods; Insect Proteins/genetics; Insect Proteins/metabolism; Proteome/metabolism; Proteomics*; Transcriptome
  2. Norazman CW, Mohd Sopian M, Lee LK
    Food Funct, 2025 Mar 03;16(5):1847-1863.
    PMID: 39930892 DOI: 10.1039/d4fo03307h
    Background: Tocotrienol has garnered significant attention due to its potent antioxidant and anti-inflammatory effects in ameliorating cardiovascular-related comorbidities. The present study aimed to elucidate the effects of tocotrienol-enriched oat supplementation on individuals with metabolic syndrome (MetS). Method: This was a randomized, double-blind, placebo-controlled human clinical trial. Patients with MetS were randomized to the tocotrienol-enriched oat (TO), oat (O) or control (C) groups. Both TO and O groups were supplemented twice daily for 12 weeks, while group C did not receive any intervention. Changes in the metabolic profile of individuals were considered as the primary endpoint. The secondary endpoints included the morphological assessment of nutritional and anthropometric parameters and health-related quality of life. Other measurements included compliance and tolerability to the study regime. Results: The rate of MetS remission in the TO and O groups was approximately twice than that in the control group (37.0% vs. 18.5%). After 12 weeks, the TO group showed significant improvements in the fasting blood glucose (-4.5%), blood pressure (systolic: -4.2%; diastolic: -5.3%), high density lipoprotein-cholesterol (HDL-C) (+34.1%), and triglyceride (-7.1%) (p < 0.05) levels. Group TO demonstrated an increase in muscle mass (+0.301 kg, p < 0.05) and reduced body fat (-0.775%, p < 0.05). Both the TO and O groups showed improvements in the overall HR-QoL, and the visual analogue scale (VAS) score. Conclusion: Twelve weeks of tocotrienol-enriched oat supplementation improved surrogate endpoints associated with MetS. This complementary dietary management approach may be more effective at alleviating MetS symptoms than the pharmacological approach alone and could be a safe dietary strategy for secondary prevention.
    MeSH terms: Adult; Blood Glucose/metabolism; Blood Pressure/drug effects; Double-Blind Method; Female; Humans; Male; Middle Aged; Nutritional Status*; Quality of Life*; Triglycerides/blood; Dietary Supplements*; Metabolome
  3. Rahman AMA, Bakar ARA, Yee AQ, Zainudin MAM, Daud NMAN, Gunny AAN, et al.
    RSC Adv, 2025 Feb 06;15(6):4296-4321.
    PMID: 39931390 DOI: 10.1039/d5ra00097a
    The present review attempts to evaluate the applicability of deep eutectic solvents (DES) as a green technique for the extraction of phytochemicals from Mangifera indica L. and their therapeutic potential. Mango has been reported to show numerous therapeutic activities, which are attributed to its abundant source of bioactive compounds. Thus, the therapeutic potential of phytochemicals in mangoes is reviewed based on different reported bioactivity tests. The use of DESs is considered a green approach for the extraction of bioactive compounds from natural sources utilizing two or more components and a safe alternative for application in the nutritional, pharmaceutical and other sectors. The trends in the extraction of phytochemicals from mango using different DES components and different extraction parameters of the optimum protocol are reviewed. Hence, DESs are considered potential solvents with selective and efficient properties for extracting bioactive ingredients from mango. However, there are several knowledge gaps that need to be assessed for DES-based bioactive compound extraction from mango such as information on the local and specific varieties of mangoes, standardization of the extraction protocols and use of other parts of the mango plant as alternatives to its peel as bioactive sources. Accordingly, the extraction of bioactive compounds from mango using DESs will provide useful information for subsequent agricultural, pharmaceutical and nutraceutical applications in the future.
  4. Halim R, Kaur M, Syed Mokhtar SS, Chemi N, Sajatovic M, Tan YK, et al.
    Psychol Res Behav Manag, 2025;18:209-223.
    PMID: 39931525 DOI: 10.2147/PRBM.S485333
    BACKGROUND: Internalized stigma and medication non-adherence pose significant challenges for treating major depressive disorder (MDD), leading to disability, increased suicide risk, and morbidity. Limited data exists on modifiable factors associated with adherence in lower-resourced settings like Malaysia. This study aimed to investigate poor adherence prevalence and the demographic and clinical factors associated with poor medication adherence among patients with MDD.

    METHODS: This cross-sectional survey recruited participants using universal sampling from a major hospital in Selangor, Malaysia. Participants answered questionnaires consisting of demographic and clinical information (medical history, duration of anti-depressant treatment for MDD, psychiatric ward admission history, and any medication side effects experienced), the Internalized Stigma of Mental Illness Scale (comprising alienation, stereotype, perceived discrimination, and social withdrawal), the Multidimensional Scale of Perceived Social Support (comprising family, friend, and significant other support), and the Malaysia Medication Adherence Assessment Tool. Simple and multiple logistic regression and mediation analyses were conducted.

    RESULTS: Of the 268 participants (69% female), 57.1% were suboptimally or moderately adherent to their medication. Buddhists (adjusted odds ratio [aOR] = 0.280, 95% CI [0.115, 0.679], p = 0.005), higher family support (aOR = 0.753, 95% CI [0.591, 0.960], p = 0.022), a history of ward admissions (aOR = 3.523, 95% CI [1.537, 8.072], p = 0.003), and higher internalized stigma (aOR = 2.828, 95% CI [1.497, 5.344], p = 0.001) were significantly associated with low/moderate medication adherence. The effect of internalized stigma subdomains (alienation, perceived discrimination, and social withdrawal) on medication adherence were partially mediated by family support.

    CONCLUSION: More than half of the participants demonstrated low to moderate medication adherence. Those with higher internalized stigma demonstrated higher odds of low/moderate medication adherence, but this effect was attenuated by family support. Therefore, internalized stigma and family support are important points of consideration when assessing patients with MDD in Malaysia.

  5. Kee LT, Foo JB, How CW, Nur Azurah AG, Chan HH, Mohd Yunus MH, et al.
    Int J Nanomedicine, 2025;20:1561-1578.
    PMID: 39931529 DOI: 10.2147/IJN.S497940
    INTRODUCTION: Research has unveiled the remarkable properties of extracellular vesicles derived from mesenchymal stromal cells (MSCs), particularly in promoting wound healing, aiding re-epithelialization, revitalizing aging skin, and inhibiting hyperpigmentation. However, investigations into the potential of small extracellular vesicles from umbilical cord-derived MSCs (UC-MSC-sEVs) in reducing scarring and preventing hyperpigmentation remain limited. Therefore, this study aims to evaluate the impact of UC-MSC-sEVs on the synthesis of the skin's extracellular matrix (ECM) and pigmentation using in vitro models.

    METHODS: The study investigated the impact of characterized UC-MSC-sEVs on various aspects including the proliferation, migration, antioxidant activity, and ECM gene expression of human dermal fibroblasts (HDF). Additionally, the effects of UC-MSC-sEVs on the proliferation, melanin content, and tyrosinase (TYR) activity of human melanoma cells (MNT-1) were examined. Furthermore, ex vivo models were employed to evaluate the skin permeation of PKH26-labelled UC-MSC-sEVs.

    RESULTS: The findings indicated that a high concentration of UC-MSC-sEVs positively influenced the proliferation of HDF. However, no changes in cell migration rate were observed. While the expressions of collagen type 1 and type 3 remained unaffected by UC-MSC-sEVs treatment, there were dose-dependent increases in the gene expressions of fibronectin, matrix metallopeptidase (MMP) 1, and MMP 3. Furthermore, UC-MSC-sEVs treatment did not impact the antioxidative superoxide dismutase (SOD) expression in HDF. Although UC-MSC-sEVs did not alter the proliferation of MNT-1 cells, it did result in a dose-dependent reduction in melanin synthesis without affecting TYR activity. However, when it was applied topically, UC-MSC-sEVs failed to penetrate the skin barrier and remained localized within the stratum corneum layer even after 18 hours.

    CONCLUSION: These results highlight the potential of UC-MSC-sEVs in stimulating HDF proliferation, regulating ECM synthesis, and reducing melanin production. This demonstrates the promising application of UC-MSC-sEVs in medical aesthetics for benefits such as scar reduction, skin rejuvenation, and skin lightening.

    MeSH terms: Animals; Cell Movement*; Cells, Cultured; Fibroblasts/metabolism; Humans; Skin Pigmentation; Monophenol Monooxygenase/metabolism; Cell Line, Tumor; Cell Proliferation*
  6. Al Shakhori M, Arain S, Abdulsalim S, Salim Karattuthodi M, Al Dhamen M, Almutairi S, et al.
    J Pharm Policy Pract, 2025;18(1):2460038.
    PMID: 39931673 DOI: 10.1080/20523211.2025.2460038
    BACKGROUND: The study aimed to evaluate the effectiveness of telepsychiatry combined with the expertise of psychiatric clinical pharmacists in identifying and addressing drug-related problems (DRPs) associated with psychotropic medications. Additionally, the research assessed physicians' acceptance of pharmacists' recommendations for managing these DRPs.

    METHODS: A cohort retrospective study was conducted at a leading tertiary care hospital in Saudi Arabia spanning from January 2023 to January 2024 in a psychiatry setting. The study comprehensively examined all instances of interventions for DRPs facilitated through patient-initiated telepsychiatry encounters with psychiatric clinical pharmacists. Detailed and meticulously recorded notes from patient chart reviews, documented by the pharmacist in the Electronic Health Record (EHR), during each encounter, were reviewed. These notes provided significant information on psychiatric diagnosis, identified DRPs and the specific interventions and recommendations proposed by the clinical pharmacist to the attending physician. The Krska classification was utilised to classify and analyse the identified DRPs, ensuring a structured and systematic approach to the study's findings.

    RESULTS: A total of 259 pharmacist interventions were made, and the results revealed a remarkably high acceptance rate of 98.5% among physicians. The most common intervention (16.21%) involved targeted education to improve medication adherence. Additionally, substantial efforts were directed towards rectifying inappropriate dosage regimens, accounting for 13.51% of DRPs resolved by the pharmacist. Noteworthy interventions also encompassed the identification and management of potential or suspected adverse reactions, comprising 12.35% of the interventions, along with interventions addressing concerns regarding potentially ineffective therapy, which constituted 11.59%.

    CONCLUSION: The study underscores the critical role of pharmacists in psychiatric care, with high physician acceptance of their interventions. The diverse range of DRPs highlights the need to expand clinical pharmacy services and integrate pharmacists into psychiatric teams. Our findings clearly demonstrate that integrating pharmacists into psychiatric care settings is beneficial. This approach enhances DRP identification and management, ultimately enhancing patient care and treatment outcomes.

  7. Gopinath D, On CY, Veettil SK, Tilakaratne WM
    Oral Dis, 2025 Jan;31(1):59-68.
    PMID: 39503340 DOI: 10.1111/odi.15160
    BACKGROUND: This meta-analysis summarizes the current evidence on the intra- and inter-observer agreement between WHO and the binary grading systems used to assess epithelial dysplasia (ED).

    METHODS: A systematic search for observational studies that compared the level of agreement among pathologists between WHO and binary grading systems for ED was conducted using three databases: Medline, Scopus, and EBSCOhost. For the meta-analysis, summary estimations of kappa value (κ) and standard error (SE) were utilized.

    RESULTS: The pooled analysis of observations by 46 pathologists from a total of eight studies showed better interobserver agreement in the interpretation of ED for the binary system (κ = 0.31; 95% confidence interval [CI], 0.23-0.40) in comparison with the WHO (κ = 0.14; 95% CI, 0.10-0.19). The intra-observer agreement was reported only by five studies and was also found to be higher for the binary system (κ = 0.44; 95% CI, 0.31-0.57) compared to the WHO (κ = 0.25; 95% CI, 0.11-0.39).

    CONCLUSIONS: Our results validate that the binary system has better overall intra-observer and interobserver agreement than the WHO system. Further studies with larger cohorts are mandatory before clinically relevant conclusions are drawn, as evidence remains inadequate.

    MeSH terms: Humans; World Health Organization; Observer Variation*; Neoplasm Grading
  8. Spector D, Bykusov V, Isaeva Y, Akasov R, Zharova A, Rodin I, et al.
    ChemMedChem, 2025 Jan 10.
    PMID: 39791228 DOI: 10.1002/cmdc.202400786
    Light induced release of cisplatin from Pt(IV) prodrugs is a promising tool for precise spatiotemporal control over the antiproliferative activity of Pt-based chemotherapeutic drugs. A combination of light-controlled chemotherapy (PACT) and photodynamic therapy (PDT) in one molecule has the potential to overcome crucial drawbacks of both Pt-based chemotherapy and PDT via a synergetic effect. Herein we report green-light-activated Pt(IV) prodrug GreenPt with BODIPY-based photosentitizer in the axial position with an incredible high light response and singlet oxygen generation ability. GreenPt demonstrated the ability to release cisplatin under low-dose green light irradiation up to 1 J/cm2. The investigation of the photoreduction mechanism of GreenPt prodrug using DFT modeling and ΔG0 PET estimation revealed that the anion-radical formation and substituent photoinduced electron transfer from the triplet excited state of the BODIPY axial ligand to the Pt(IV) center is the key step in the light-induced release of cisplatin. Green-light-activated BODIPY-based photosentitizers 5 and 8 demonstrated outstanding photosensitizing properties with an extraordinary phototoxicity index (PI)>1300. GreenPt prodrug demonstrated gradual intracellular accumulation and light-induced phototoxicity with PI>100, thus demonstrating dual action through light-controlled release of both cisplatin and a potent BODIPY-based photosensitizer.
  9. Al-Mijalli SH, Mrabti HN, Abdallah EM, Assaggaf H, Qasem A, Alenazy R, et al.
    Microb Pathog, 2025 Mar;200:107357.
    PMID: 39894234 DOI: 10.1016/j.micpath.2025.107357
    Acorus calamus is a medicinal plant known for its multifaceted health benefits, especially against inflammation and infectious ailments. In the current work, we attempt to investigate the volatile constituents of A. calamus essential oil (ACEO) and, for the first time, to elucidate its antibacterial mechanism. Gas chromatography coupled to a mass spectrometer (GC-MS) was used to investigate phytochemical substances. The GC-MS analysis detected β-asarone (71.13 %), an aromatic anisole as main component, followed by α-asarone (12.07 %), β-calacorene (3.01 %), methyl isoeugenol (2.16 %). ACEO exhibited remarkable antibacterial activity, as demonstrated by significant inhibition zones against several bacteria that were tested. Gram-positive strains, Staphylococcus aureus and Bacillus subtilis, showed inhibition zones measuring 20.11 ± 0.28 mm and 18.06 ± 1.36 mm, respectively, while Gram-negative bacteria, Pseudomonas aeruginosa, and Escherichia coli, displayed slightly smaller zones of 15.58 ± 0.68 mm and 16.00 ± 0.04 mm. The results of ACEO were competitive with Tetracycline and Cefoperazone. Furthermore, ACEO demonstrated low MICs (ranging from 0.125 % to 1.0 %) and MBCs (ranging between 0.125 % and 2.0 %), with MBC/MIC ratios consistently below 4.0, confirming its bactericidal nature. Time-kill kinetics represented high lethality of ACEO at MIC levels against Staphylococcus aureus and Pseudomonas aeruginosa, resulting in a significant reduction in colony-forming units within 12-24 h. The antibacterial mechanisms of ACEO were investigated, demonstrating its potential to disrupt the integrity of the cell membrane and enhance membrane permeability. This disruption results in the release of genetic material (RNA and DNA) and proteins from the bacterial cell. Significant anti-biofilm activity further highlights the potential of the tested compounds in combating biofilm-associated infections. Scanning electron microscopy (SEM) images revealed significant inhibition of bacterial adhesion (First step of biofilm formation) on used surface as well as possible morphological changes in bacterial cells treated with ACEO.
    MeSH terms: Anisoles/pharmacology; Anisoles/chemistry; Gas Chromatography-Mass Spectrometry*; Microbial Sensitivity Tests*; Plant Extracts/pharmacology; Plant Extracts/chemistry; Biofilms/drug effects; Volatile Organic Compounds/analysis; Volatile Organic Compounds/pharmacology; Volatile Organic Compounds/chemistry
  10. Mobley AM, Wegman MP, Bazazi AR, Shenoi SV, Bromberg DJ, Ahmad A, et al.
    Int J Drug Policy, 2025 Mar;137:104733.
    PMID: 39923270 DOI: 10.1016/j.drugpo.2025.104733
    PURPOSE: The transition from prison is hazardous, especially for people with HIV and opioid use disorder. To determine the impact of methadone on linkage to HIV care in people with HIV and opioid use disorder, we prospectively compared those allocated to pre-release methadone or not.

    METHODS: A prospective, open-label trial of 310 people with HIV and opioid use disorder at Malaysia's largest prison were allocated to pre-release methadone up to 24 weeks before release or not by randomization (n = 64) or preference (n = 246); 296 were included in the final analytical sample. Directed acyclic graphing was used to theorize the relationship between pre-release methadone and post-release linkage to HIV care and identify confounding variables. An inverse probability weighted Cox proportional hazards model estimated the impact of pre-release methadone on linkage to HIV care through 360 days after release.

    RESULTS: Overall, 218 (73.6 %) of 296 study participants initiated methadone before release. Receiving pre-release methadone significantly predicted linkage to HIV care at all time points through 360 days (aHR = 1.87; 95 % CI 1.15-2.85) after release. The corresponding numbers needed to treat with pre-release methadone for one increased linkage to HIV care at 30 and 360 days were 14 (95 % CI 9.2-62.4) and 5 (95 % CI 3.4-22.0), respectively.

    CONCLUSIONS: While treatment with methadone should be available to everyone with opioid use disorder, it should especially be included as part of an HIV treatment-as-prevention strategy for people in prisons, especially by the time of release. It can optimize HIV treatment outcomes by jumpstarting the HIV treatment cascade.

    MeSH terms: Adult; Female; Humans; Malaysia; Male; Middle Aged; Prisons; Prospective Studies; Patient Preference*; Opiate Substitution Treatment*
  11. Almugren KS, Sabtu SN, Sani SFA, Dzamrah NH, Shahira MNN, Shafiqah ASS, et al.
    Appl Radiat Isot, 2025 Apr;218:111699.
    PMID: 39923337 DOI: 10.1016/j.apradiso.2025.111699
    This study explored the thermoluminescence (TL) properties of various quartz types (amethyst, citrine, rock crystal, and rose quartz) when exposed to gamma irradiation, assessing their potential for dosimetric applications. Key aspects such as heating rate, glow curves, dose-response behavior, linearity index, sensitivity, and fading characteristics were analyzed for each quartz type. The results revealed significant differences in TL performance among the quartz samples, with each type exhibiting distinct characteristics under gamma irradiation. Amethyst displayed the most reliable TL behavior, with strong linearity and stable dose-response relationships, making it the most suitable candidate for radiation dosimetry. These findings contribute valuable insights into the selection of optimal quartz materials for radiation measurement, enhancing the precision and reliability of TL-based dosimetric techniques.
  12. Ru Y, Norlizah HC, Nasuha Burhanuddin NA, Liu H, Dong J
    Addict Behav, 2025 May;164:108272.
    PMID: 39923383 DOI: 10.1016/j.addbeh.2025.108272
    OBJECTIVES: In the modern era of constant digital technological advancements, problematic smartphone use has become a key issue in educational psychology. This phenomenon affects individual psychological well-being and academic or other performance and encompasses interpersonal relationships and societal aspects, divulging the intricate correlation between technology usage and human behavior. This study aims to explore mindfulness as an intervention measure to lessen problematic smartphone use.

    METHODS: Based on the PRISMA method, this study used a meta-analytic approach to explore the relationship between mindfulness and problematic smartphone use systematically. Systematic literature searches for papers published before February 2024 yielded 29 studies, including 17,534 individuals and 30 effect sizes. The sample comprised participants from Eastern cultures (n = 22 studies) and Western cultures (n = 8 studies).

    RESULTS: The meta-analysis revealed a significant negative correlation between mindfulness and problematic smartphone use (r = -0.399, 95 % CI [-0.457, -0.338], p 

    MeSH terms: Smartphone; Adolescent; Adult; Child; Female; Humans; Male; Mindfulness*
  13. Zainol DA, Rahumatullah A, Anuar NS, Raaj S
    Clin Chim Acta, 2025 Mar 01;569:120184.
    PMID: 39923908 DOI: 10.1016/j.cca.2025.120184
    Strongyloides stercoralis infection, a neglected tropical disease, poses a significant public health threat, especially in immunocompromised individuals. This parasitic nematode can establish chronic infections, potentially progressing to life-threatening conditions such as hyperinfection syndrome and disseminated disease. Timely and accurate diagnosis is critical for effective treatment and the prevention of severe complications. Traditional diagnostic methods, such as stool microscopy, are limited by low sensitivity, particularly for detecting low-intensity infections. Advances in molecular diagnostics, particularly Polymerase Chain Reaction (PCR), have significantly improved sensitivity and specificity, marking a pivotal shift in detection capabilities. However, critical barriers persist, including inconsistencies in sample collection and handling, geographic variations in parasite strains, and the impact of genetic diversity on assay performance. Emerging molecular technologies, such as real-time PCR, loop-mediated isothermal amplification (LAMP), and droplet digital PCR (ddPCR) hold significant promise for further enhancing diagnostic precision. These advanced methods provide opportunities for more robust and accessible diagnostics, particularly in resource-limited settings. To maximize their potential, it is imperative to address existing challenges through the standardization of protocols, optimization of sample handling procedures, and the development of high-quality, reliable reagents. By overcoming these obstacles, molecular diagnostics can be more effectively integrated into clinical and public health frameworks, facilitating improved management and control of S. stercoralis infection, ultimately reducing the morbidity and mitigating the global burden of this neglected tropical disease.
    MeSH terms: Animals; Humans; Nucleic Acid Amplification Techniques/methods; Molecular Diagnostic Techniques*
  14. Lee H
    JMIR Form Res, 2025 Feb 07;9:e62935.
    PMID: 39924308 DOI: 10.2196/62935
    BACKGROUND: The Alma-Ata Declaration of 1978 initiated a global focus on universal health, supported by the World Health Organization (WHO) through healthy cities policies. The concept emerged at the 1984 Toronto "Beyond Health Care" conference, leading to WHO's first pilot project in Lisbon in 1986. The WHO continues to support regional healthy city networks, emphasizing digital transformation and data-driven health management in the digital era.

    OBJECTIVE: This study explored the capabilities of digital healthy cities within the framework of digital transformation, focusing on member countries of the Asian Forum of Healthy Cities. It examined the cities' preparedness and policy needs for transitioning to digital health.

    METHODS: A cross-sectional survey was conducted of 9 countries-Australia, Cambodia, China, Japan, South Korea, Malaysia, Mongolia, the Philippines, and Vietnam-from August 1 to September 21, 2023. The 6-section SPIRIT (setting approach and sustainability; political commitment, policy, and community participation; information and innovation; resources and research; infrastructure and intersectoral; and training) checklist was modified to assess healthy cities' digital capabilities. With input from 3 healthy city experts, the checklist was revised for digital capabilities, renaming "healthy city" to "digital healthy city." The revised tool comprises 8 sections with 33 items. The survey leveraged ChatGPT (version 4.0; OpenAI, Microsoft), accessed via Python (Python Software Foundation) application programming interface. The openai library was installed, and an application programming interface key was entered to use ChatGPT (version 4.0). The "GPT-4 Turbo" model command was applied. A qualitative analysis of the collected data was conducted by 5 healthy city experts through group deep-discussions.

    RESULTS: The results indicate that these countries should establish networks and committees for sustainable digital healthy cities. Cambodia showed the lowest access to electricity (70%) and significant digital infrastructure disparities. Efforts to sustain digital health initiatives varied, with countries such as Korea focusing on telemedicine, while China aimed to build a comprehensive digital health database, highlighting the need for tailored strategies in promoting digital healthy cities. Life expectancy was the highest in the Republic of Korea and Japan (both 84 y). Access to electricity was the lowest in Cambodia (70%) with the remaining countries having had 95% or higher access. The internet use rate was the highest in Malaysia (97.4%), followed by the Republic of Korea (97.2%), Australia (96.2%), and Japan (82.9%).

    CONCLUSIONS: This study highlights the importance of big data-driven policies and personal information protection systems. Collaborative efforts across sectors for effective implementation of digital healthy cities. The findings suggest that the effectiveness of digital healthy cities is diminished without adequate digital literacy among managers and users, suggesting the need for policies to improve digital literacy.

    MeSH terms: Artificial Intelligence; Australia; Cities*; Cross-Sectional Studies; Humans; Surveys and Questionnaires; World Health Organization
  15. Rosli SR, Radzi NDM, Razak MAA, Abdul Aziz A
    J Prosthet Dent, 2025 Feb 08.
    PMID: 39924435 DOI: 10.1016/j.prosdent.2025.01.030
    STATEMENT OF PROBLEM: Studies on the assessment of the marginal fit of interim crowns with optical coherence tomography (OCT) are lacking.

    PURPOSE: The purpose of this in vitro study was to apply OCT to evaluate and to compare the impact of conventional and computer-aided design and computer-aided manufacturing (CAD-CAM) fabrication methods and different materials, including bis-acryl, conventional polymethyl-methacrylate (PMMA), and CAD-CAM PMMA, on the marginal fit of interim crowns.

    MATERIAL AND METHODS: Thirty crown specimens were fabricated, with 20 PMMA crowns divided into 2 groups based on the fabrication method: the conventional method (Group Jet, n=10) and the CAD-CAM method (Group CAD-CAM PMMA, n=10). An additional 10 bis-acryl crowns were fabricated using the conventional method (Group Protemp, n=10). The marginal gap was assessed at 8 specific points using swept-source OCT. Comparisons between the fabrication methods of PMMA crowns and different materials of conventionally made crown were done using an independent t test (α=.05).

    RESULTS: Group CAD-CAM PMMA recorded statistically significant lower total mean vertical marginal gap values (22.2 ±4.7 µm) compared with group Jet (62.6 ±2.2 µm) (P

  16. Matrook KA, Whitford DL, Smith SM, McGilloway S, Piyasena MP, Cowman S
    Prim Care Diabetes, 2025 Apr;19(2):111-119.
    PMID: 39924437 DOI: 10.1016/j.pcd.2025.01.006
    Family-based interventions involve family members in caring for patients with diabetes, but it is unclear whether they are effective in improving outcomes for type 2 diabetes (T2DM). This systematic review and meta-analysis aim to determine the effectiveness of family-based interventions in improving glycaemic control in T2DM patients. We identified 6163 abstracts. Eleven RCTs (n = 1421) met our inclusion criteria. The overall findings suggest that family-based interventions are effective in improving HbA1c, at 3-4 months follow-up and when the intervention is delivered by nurses, with modest effects. Consideration should be given to integrating family-based interventions in the primary care among cultures with greater family cohesion.
    MeSH terms: Adult; Aged; Female; Humans; Male; Middle Aged; Primary Health Care; Time Factors; Treatment Outcome; Caregivers/psychology
  17. Detho A, Kadir AA, Shayuti A, Rahim BA, Ghazouani N, Mabrouk A, et al.
    Sci Rep, 2025 Feb 09;15(1):4820.
    PMID: 39924559 DOI: 10.1038/s41598-025-89147-1
    The increasing generation of industrial waste sludge poses a serious worldwide problem with detrimental effects on the environment and the economy. Effective utilization of waste sludge in sustainable construction practices offers a universal solution to mitigate environmental impacts. As the mining industry continues to extract clay from clay mines, the demand for sustainable practices in both clay mineral extraction and brick production is growing. Bricks are fundamental in masonry construction, and current research is exploring the integration of industrial waste materials into fired clay bricks to enhance their properties and mitigate environmental impacts. This study investigates the incorporation of waste sludge in brick manufacturing to assess its potential for reducing environmental burdens while maintaining technical performance. X-ray Fluorescence Spectrometry (XRF) analysis reveals that both clay soil and mosaic sludge contain high levels of silicon dioxide (SiO₂) and aluminum oxide (Al₂O₃), supporting their suitability as partial substitutes for clay soil. Incorporating up to 30% of body mill sludge (BS) and polishing sludge (PS) into the brick mix significantly enhances physical and mechanical properties, resulting in reduced shrinkage, increased porosity, and improved compressive strength, reaching up to 25 N/mm². Initial rate of suction tests shows values below 5 g/mm², indicating optimal water absorption characteristics. Various leachability assessments, including the Toxicity Characteristic Leaching Procedure (TCLP), Synthetic Precipitation Leaching Procedure (SPLP), and Static Leachate Test (SLT), confirm that bricks containing up to 30% BS and PS comply with United States Environmental Protection Agency (USEPA) and Environment Protection Authority Victoria (EPAV) standards for heavy metals, making them environmentally safe for use. Additionally, indoor air quality assessments confirm that these bricks meet Industry Codes of Practice on Indoor Air Quality (ICOP-IAQ) guidelines. This study demonstrates that using BS and PS as alternative raw materials offers a sustainable, cost-effective solution aligned with Sustainable Development Goals (SDGs), promoting cleaner production practices in brick manufacturing.
  18. Qin L, Ho WKY, Khoo S
    Sci Rep, 2025 Feb 09;15(1):4844.
    PMID: 39924573 DOI: 10.1038/s41598-025-89287-4
    Exercise Self-Efficacy Scale (ESES) is widely used to assess individuals' exercise self-efficacy through self-reporting. It includes one factor and 18 items that gauge one's confidence in exercising under various conditions, such as when tired, stressed, or in unfavourable weather. Evidence indicates that differences in the original factor structure and psychometric properties were observed across different populations, including school-age students, university students, and adults. This study examined the factor structure, reliability, convergent validity, and measurement invariance of the Chinese version of the ESES among secondary school students. Data from 856 students (age: M = 13.80, SD = 0.94) were analysed using exploratory and confirmatory factor analyses to identify the best-fitting factor structure. The reliability and convergent validity were tested using the collected data. Configural, metric, and scalar invariances, as well as the likelihood ratio test, were tested for measurement invariance. A 14-item, two-factor structure of the ESES, consistently demonstrated the best fit among secondary school students. The two-factor structure showed strong internal consistency reliability (McDonald's Omega of 0.921 and 0.843) and satisfactory convergent validity (average variance extracted values of 0.582 and 0.478, composite reliability values of 0.917 and 0.845). Multi-group confirmatory factor analysis (likelihood ratio test, p > 0.01) revealed scalar measurement invariance across sex, ethnic backgrounds, grades, and school locations. These findings suggest that the 14-item, two-factor Chinese version of the ESES is suitable for use with secondary-school students. Future studies could confirm these findings by examining the 14-item, two-factor ESES in diverse samples, considering ethnicity, socioeconomic status, and age range.
    MeSH terms: Adolescent; Child; China; Factor Analysis, Statistical; Female; Humans; Male; Surveys and Questionnaires; Schools; Reproducibility of Results; Self Efficacy*
  19. Salsabila S, Khairinisa MA, Wathoni N, Sufiawati I, Mohd Fuad WE, Khairul Ikram NK, et al.
    Artif Cells Nanomed Biotechnol, 2025 Dec;53(1):1-15.
    PMID: 39924869 DOI: 10.1080/21691401.2025.2462328
    Chitosan nanoparticles have been extensively utilised as polymeric drug carriers in nanoparticles formulations due to their potential to enhance drug delivery, efficacy, and safety. Numerous toxicity studies have been previously conducted to assess the safety profile of chitosan-based nanoparticles. These toxicity studies employed various methodologies, including test animals, interventions, and different routes of administration. This review aims to summarise research on the safety profile of chitosan-based nanoparticles in drug delivery, with a focus on general toxicity tests to determine LD50 and NOAEL values. It can serve as a repository and reference for chitosan-based nanoparticles, facilitating future research and further development of drugs delivery system using chitosan nanoparticles. Publications from 2014 to 2024 were obtained from PubMed, Scopus, Google Scholar, and ScienceDirect, in accordance with the inclusion and exclusion criteria.The ARRIVE 2.0 guidelines were employed to evaluate the quality and risk-of-bias in the in vivo toxicity studies. The results demonstrated favourable toxicity profiles, often exhibiting reduced toxicity compared to free drugs or substances. Acute toxicity studies consistently reported high LD50 values, frequently exceeding 5000 mg/kg body weight, while subacute studies typically revealed no significant adverse effects. Various routes of administration varied, including oral, intravenous, intraperitoneal, inhalation, and topical, each demonstrating promising safety profiles.
    MeSH terms: Animals; Drug Carriers/toxicity; Drug Carriers/chemistry; Humans
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