Browse publications by year: 2025

  1. Shanmugham S, Zuber M, Chan JE, Kumar S, Ching SM, Lee YY, et al.
    Indian J Gastroenterol, 2025 Feb;44(1):24-34.
    PMID: 39180628 DOI: 10.1007/s12664-024-01648-5
    INTRODUCTION: The beneficial effects of using antidepressants in improving functional dyspepsia (FD) symptoms have been reported in previous meta-analyses; however, the results have not been conclusive. The aim was to perform an updated meta-analysis coupled with trial sequential analysis (TSA) to assess the efficacy of the use of any antidepressants in the treatment of FD in adults.

    METHODS: Electronic databases were searched up to March 2024 for randomized controlled trials (RCTs) recruiting adults with FD. Data of overall symptoms improved between the antidepressants and placebo groups was pooled to obtain risk ratio (RR) employing the random-effects model. The effect of random errors was evaluated with TSA. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach was used to assess the certainty of evidence. Analyses were performed using STATA version 16.0.

    RESULTS: Nine RCTs with 924 patients met the eligible criteria. The RRs of FD symptoms improving with any antidepressants, tricyclic antidepressants (TCAs) and selective serotonin reuptake inhibitors were (n = 9, RR = 1.30 [95% CI, 1.02-1.67]), (n = 5, RR = 1.41 [95% CI, 1.07-1.85]) and (n = 2, RR = 0.97 [95% CI, 0.72-1.29]), respectively. TSA demonstrated conclusive evidence for the beneficial effect of TCAs. The number needed to treat (NNT) with any depressants and TCAs were 11 (95% CI, 7-36) and 6 (95% CI, 4-15), respectively. The certainty of the evidence for an effect of TCAs was that of moderate GRADE quality. The benefit, however, was limited to the western population (n = 3, RR = 1.43 [95% CI, 1.04-1.96]) and did not extend to the Asian population (n = 2, RR = 1.32 [95% CI, 0.75-2.32]). Conversely, antidepressant-using patients experienced adverse events more frequently. However, no statistically significant association was found between TCAs and any adverse events (n = 3; RR = 1.36 [95% CI, 0.91-2.04]).

    CONCLUSION: Evidence was obtained suggesting TCAs can be an effective alternative in the treatment of FD, but more evidence from high-quality large trials is required to support their use, especially in the Asian population.

    MeSH terms: Adult; Antidepressive Agents, Tricyclic/therapeutic use; Female; Humans; Male; Randomized Controlled Trials as Topic*; Treatment Outcome; Serotonin Uptake Inhibitors/therapeutic use
  2. Yusof Z, Lim V, Khong NMH, Choo WS, Foo SC
    J Sci Food Agric, 2025 Jan 15;105(1):93-108.
    PMID: 39177277 DOI: 10.1002/jsfa.13807
    BACKGROUND: The present study evaluated the effects of temperature, pH, light and chemical oxidation on fucoxanthin changes in terms of colour, antioxidant activity and metabolomic profile. Additionally, the correlation between antioxidant activity and identified metabolites was analysed.

    RESULTS: It was found that colour change was significantly reduced at elevated heat (100 °C, *∆E = 0.81 ± 0.05), reduced pH (pH 3, *∆E = 0.59 ± 0.04) and length of light exposure (*∆E = 3.16 ± 0.04). Antioxidant activity decreased under all treatments. Among the temperatures tested, fucoxanthin exhibited the highest activity at 60 °C, ranging from 0.92 to 3.04 mg Trolox equivalents (TE) g-1. Significant activity reductions (P 

    MeSH terms: Color*; Hot Temperature; Hydrogen-Ion Concentration; Light*; Temperature*
  3. Mohamed Yusoff AA, Mohd Khair SZN
    Rev Neurosci, 2025 Jan 29;36(1):53-90.
    PMID: 39174305 DOI: 10.1515/revneuro-2024-0080
    Neurodegenerative diseases represent a significant challenge to modern medicine, with their complex etiology and progressive nature posing hurdles to effective treatment strategies. Among the various contributing factors, mitochondrial dysfunction has emerged as a pivotal player in the pathogenesis of several neurodegenerative disorders. This review paper provides a comprehensive overview of how mitochondrial impairment contributes to the development of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, Huntington's disease, and amyotrophic lateral sclerosis, driven by bioenergetic defects, biogenesis impairment, alterations in mitochondrial dynamics (such as fusion or fission), disruptions in calcium buffering, lipid metabolism dysregulation and mitophagy dysfunction. It also covers current therapeutic interventions targeting mitochondrial dysfunction in these diseases.
    MeSH terms: Animals; Humans; Mitochondrial Diseases/metabolism; Mitochondrial Dynamics/physiology; Mitochondrial Degradation/physiology
  4. Ozcelik IB, Kabakas F, Mersa B, Purisa H, Sezer I, Jusoh MH
    J Hand Surg Eur Vol, 2025 Feb;50(2):208-213.
    PMID: 39169767 DOI: 10.1177/17531934241264651
    III.
    MeSH terms: Adolescent; Adult; Amputation, Traumatic/surgery; Anastomosis, Surgical*; Female; Humans; Male; Middle Aged; Retrospective Studies; Veins/surgery; Young Adult
  5. Sakuma H, Thomas T, Debinski C, Eyre M, Han VX, Jones HF, et al.
    Dev Med Child Neurol, 2025 Feb;67(2):195-207.
    PMID: 39143740 DOI: 10.1111/dmcn.16067
    AIM: To develop standardized diagnostic criteria for 'infection-triggered encephalopathy syndrome (ITES)' and five specific clinical syndromes of ITES.

    METHOD: The draft definitions were based on existing criteria, standardized, and discussed by a panel of international experts using nominal group technique over 18 months to achieve consensus. All criteria use the same format: (1) presence of infection/fever; (2) clinical features including encephalopathy; (3) neuroradiological features on magnetic resonance imaging; (4) exclusion of other causes.

    RESULTS: We first highlighted differences between ITES and infectious and autoimmune encephalitis, which is the most important differential diagnosis. Consensus was achieved to define five specific ITESs: acute encephalopathy with biphasic seizures and late reduced diffusion; acute necrotizing encephalopathy; mild encephalopathy with a reversible splenial lesion; acute fulminant cerebral oedema; and acute shock with encephalopathy and multiorgan failure. Two further conditions that are currently classified as epilepsy syndromes but have similar features to ITES, namely febrile infection-related epilepsy syndrome and hemiconvulsion-hemiplegia-epilepsy syndrome, are also discussed.

    INTERPRETATION: The consensus definition is expected to improve awareness of this disease concept, provide diagnostic framework, and facilitate future international research and clinical trials.

    MeSH terms: Diagnosis, Differential; Encephalitis/complications; Encephalitis/diagnosis; Humans; Magnetic Resonance Imaging; Syndrome; Consensus*
  6. Brenner T, Kuo A, Sperna Weiland CJ, Kamal A, Elmunzer BJ, Luo H, et al.
    Gastrointest Endosc, 2025 Jan;101(1):129-138.e0.
    PMID: 39147103 DOI: 10.1016/j.gie.2024.08.009
    BACKGROUND AND AIMS: A robust model of post-ERCP pancreatitis (PEP) risk is not currently available. We aimed to develop a machine learning-based tool for PEP risk prediction to aid in clinical decision making related to periprocedural prophylaxis selection and postprocedural monitoring.

    METHODS: Feature selection, model training, and validation were performed using patient-level data from 12 randomized controlled trials. A gradient-boosted machine (GBM) model was trained to estimate PEP risk, and the performance of the resulting model was evaluated using the area under the receiver operating curve (AUC) with 5-fold cross-validation. A web-based clinical decision-making tool was created, and a prospective pilot study was performed using data from ERCPs performed at the Johns Hopkins Hospital over a 1-month period.

    RESULTS: A total of 7389 patients were included in the GBM with an 8.6% rate of PEP. The model was trained on 20 PEP risk factors and 5 prophylactic interventions (rectal nonsteroidal anti-inflammatory drugs [NSAIDs], aggressive hydration, combined rectal NSAIDs and aggressive hydration, pancreatic duct stenting, and combined rectal NSAIDs and pancreatic duct stenting). The resulting GBM model had an AUC of 0.70 (65% specificity, 65% sensitivity, 95% negative predictive value, and 15% positive predictive value). A total of 135 patients were included in the prospective pilot study, resulting in an AUC of 0.74.

    CONCLUSIONS: This study demonstrates the feasibility and utility of a novel machine learning-based PEP risk estimation tool with high negative predictive value to aid in prophylaxis selection and identify patients at low risk who may not require extended postprocedure monitoring.

    MeSH terms: Clinical Decision-Making; Machine Learning*; Administration, Rectal; Aged; Female; Fluid Therapy/methods; Humans; Male; Middle Aged; Pancreatic Ducts; Pilot Projects; Prospective Studies; Risk Factors; ROC Curve; Stents; Risk Assessment; Point-of-Care Systems; Area Under Curve
  7. Wang S, Qiao Z, Li Z, Zhang Y, Cheng A, Zhu B, et al.
    Soft Robot, 2025 Feb;12(1):1-12.
    PMID: 39078729 DOI: 10.1089/soro.2023.0212
    Soft actuators offer numerous potential applications; however, challenges persist in achieving a high driving force and fast response speed. In this work, we present the design, fabrication, and analysis of a soft pneumatic bistable actuator (PBA) mimicking jellyfish subumbrellar muscle motion for waterjet propulsion. Drawing inspiration from the jellyfish jet propulsion and the characteristics of bistable structure, we develop an elastic band stretch prebending PBA with a simple structure, low inflation cost, exceptional driving performance, and stable driving force output. Through a bionic analysis of jellyfish body structure and motion, we integrate the PBA into a jellyfish-like prototype, enabling it to achieve jet propulsion. To enhance the swimming performance, we introduce a skin-like structure for connecting the soft actuator to the jellyfish-like soft robot prototype. This skin-like structure optimizes the fluid dynamics during jet propulsion, resulting in improved efficiency and maneuverability. Our study further analyzes the swimming performance of the jellyfish-like prototype, demonstrating a swimming speed of 3.8 cm/s (0.32 body length/s, BL/s) for the tethered prototype and 4.7 cm/s (0.38 BL/s) for the untethered prototype. Moreover, we showcase the jellyfish-like prototype's notable load-bearing capacity and fast-forward swimming performance compared to other driving methods for underwater biomimetic robots. This work provides valuable insights for the development of highly agile and fast responsive soft robots that imitate the subumbrellar muscle of jellyfish for efficient water-jet propulsion, utilizing skin-like structures to enhance swimming performance.
    MeSH terms: Animals; Biomechanical Phenomena/physiology; Equipment Design*; Biomimetics/instrumentation; Biomimetic Materials; Hydrodynamics
  8. Akyuz E, Arulsamy A, Aslan FS, Sarisözen B, Guney B, Hekimoglu A, et al.
    Mol Neurobiol, 2025 Feb;62(2):1631-1674.
    PMID: 39012443 DOI: 10.1007/s12035-024-04333-y
    Alzheimer's disease (AD) is a progressive neurodegenerative disease. The accumulation of amyloid-β (Aβ) plaques and tau neurofibrillary tangles are the key players responsible for the pathogenesis of the disease. The accumulation of Aβ plaques and tau affect the balance in chemical neurotransmitters in the brain. Thus, the current review examined the role of neurotransmitters in the pathogenesis of Alzheimer's disease and discusses the alterations in the neurochemical activity and cross talk with their receptors and transporters. In the presence of Aβ plaques and neurofibrillary tangles, changes may occur in the expression of neuronal receptors which in turn triggers excessive release of glutamate into the synaptic cleft contributing to cell death and neuronal damage. The GABAergic system may also be affected by AD pathology in a similar way. In addition, decreased receptors in the cholinergic system and dysfunction in the dopamine neurotransmission of AD pathology may also contribute to the damage to cognitive function. Moreover, the presence of deficiencies in noradrenergic neurons within the locus coeruleus in AD suggests that noradrenergic stimulation could be useful in addressing its pathophysiology. The regulation of melatonin, known for its effectiveness in enhancing cognitive function and preventing Aβ accumulation, along with the involvement of the serotonergic system and histaminergic system in cognition and memory, becomes remarkable for promoting neurotransmission in AD. Additionally, nitric oxide and adenosine-based therapeutic approaches play a protective role in AD by preventing neuroinflammation. Overall, neurotransmitter-based therapeutic strategies emerge as pivotal for addressing neurotransmitter homeostasis and neurotransmission in the context of AD. This review discussed the potential for neurotransmitter-based drugs to be effective in slowing and correcting the neurodegenerative processes in AD by targeting the neurochemical imbalance in the brain. Therefore, neurotransmitter-based drugs could serve as a future therapeutic strategy to tackle AD.
    MeSH terms: Animals; Humans; Synaptic Transmission*
  9. Cheong JM, A Halim NA, Mohammad N, Sivagnanam M, Mohd Ibrahim MS
    Eur J Dent, 2025 Feb;19(1):214-219.
    PMID: 39013444 DOI: 10.1055/s-0044-1787005
    OBJECTIVES:  This study aimed to establish the cephalometric norms of H angle soft tissue and H angle hard tissue of Malaysian Malay adult females, and to evaluate the correlation between H angles and visual perception in skeletal Class I Malay adult females.

    MATERIALS AND METHODS:  Eighty-five lateral cephalograms of skeletal Class I (mean ANB value = 3.15 ± 0.77) Malaysian Malay female patients aged 20 to 40 years (mean age = 28.6 ± 5.86 degrees) taken from October 2017 to December 2021 were measured for H angle soft tissue and H angle hard tissue. Twenty silhouettes were then converted from cephalometric films and were rated according to facial convexity/concavity by 20 laypersons, with re-evaluation after 2 weeks for intra- and interexaminer reliabilities.

    RESULTS:  The means of the H angle soft tissue and H angle hard tissue were 15.75 ± 4.16 degrees and 11.64 ± 4.71 degrees, respectively. The intraexaminer reliability test for visual perception ranged from -0.89 to 0.99 indicating poor to excellent reliability, whereas the interexaminer reliability test was 0.82 indicating good reliability. A highly statistically significant association between the H angle soft tissue and H angle hard tissue (r = 0.938; p 

  10. Farag A, Visvanathan S, Bachelez H, Morita A, Lebwohl MG, Barker JN, et al.
    J Invest Dermatol, 2025 Mar;145(3):573-582.e8.
    PMID: 39004117 DOI: 10.1016/j.jid.2024.05.034
    EFFISAYIL 1 was a randomized, placebo-controlled study of spesolimab, an anti-IL-36 receptor antibody, in patients presenting with a generalized pustular psoriasis flare. Treatment with spesolimab led to more rapid pustular and skin clearance versus treatment with placebo in approximately half of the patients. In this study, we present histologic, transcriptomic, and proteomic analyses of lesional and nonlesional skin and whole-blood samples collected from EFFISAYIL 1. Treatment with spesolimab led to a transition toward a nonlesional profile, with a downregulation of gene expressions in the skin of IL-36 transcripts (IL36α, IL36β, IL36γ) and those associated with neutrophil recruitment (CXCL1, CXCL6, CXCL8), proinflammatory cytokines (IL6, IL19, IL20), and skin inflammation (DEFB4A, S100A7, S100A8). Changes were manifest at week 1 and sustained to week 8. At the systemic level, reductions in serum biomarkers of inflammation (IL-17, IL-8, IL-6) were sustained until 12 weeks after spesolimab treatment. Considerable overlap was observed in the spesolimab-induced changes in gene and protein expressions from skin and blood samples, demonstrating the molecular basis of the effects of spesolimab on controlling local and systemic inflammation. Data are consistent with the mode of action of spesolimab, whereby inhibition of the IL-36 pathway leads to subsequent reductions in the key local and systemic pathologic events associated with generalized pustular psoriasis flares.
    MeSH terms: Adult; Double-Blind Method; Female; Humans; Inflammation/drug therapy; Interleukin-1/antagonists & inhibitors; Interleukin-1/metabolism; Male; Middle Aged; Biomarkers/blood; Cytokines/metabolism; Treatment Outcome; Proteomics/methods
  11. Humayun S, Hayyan M, Alias Y
    J Environ Sci (China), 2025 Jan;147:688-713.
    PMID: 39003083 DOI: 10.1016/j.jes.2023.11.021
    Innately designed to induce physiological changes, pharmaceuticals are foreknowingly hazardous to the ecosystem. Advanced oxidation processes (AOPs) are recognized as a set of contemporary and highly efficient methods being used as a contrivance for the removal of pharmaceutical residues. Since reactive oxygen species (ROS) are formed in these processes to interact and contribute directly toward the oxidation of target contaminant(s), a profound insight regarding the mechanisms of ROS leading to the degradation of pharmaceuticals is fundamentally significant. The conceptualization of some specific reaction mechanisms allows the design of an effective and safe degradation process that can empirically reduce the environmental impact of the micropollutants. This review mainly deliberates the mechanistic reaction pathways for ROS-mediated degradation of pharmaceuticals often leading to complete mineralization, with a focus on acetaminophen as a drug waste model.
    MeSH terms: Pharmaceutical Preparations/metabolism; Oxidation-Reduction; Water Pollutants, Chemical/chemistry
  12. Bautista JAL, Liu CM, Ibrahim AE, Lo LW, Chung FP, Hu YF, et al.
    Heart Rhythm, 2025 Jan;22(1):49-56.
    PMID: 38997056 DOI: 10.1016/j.hrthm.2024.06.062
    BACKGROUND: Prior studies have investigated cardiac anatomy and clinical parameters as predictors for pulmonary vein and non-pulmonary vein triggers.

    OBJECTIVE: We aimed to assess the link between the descending aorta to left inferior pulmonary vein (Dao-LIPV) distance and the occurrence of triggers and drivers in atrial fibrillation (AF) ablation procedures.

    METHODS: Drug-refractory AF patients who underwent first-time index catheter ablation from January 2010 to December 2019 were retrospectively assembled. The Dao-LIPV distance was measured from preablation pulmonary vein computed tomography. Patients were assigned to groups on the basis of the presence of LIPV triggers or drivers. Multivariate logistic regression was used to identify risk factors.

    RESULTS: A total of 886 consecutive patients with drug-refractory AF were studied, and 63 (7.1%) patients were identified to have LIPV triggers or drivers. The Dao-LIPV distance had a better predictive performance (area under the curve, 0.70) compared with persistent AF (area under the curve, 0.57). Multivariate logistic regression analysis showed that Dao-LIPV distance ≤2.5 mm (odds ratio, 3.96; 95% CI, 2.15-7.29; P < .001) and persistent AF (odds ratio, 1.73; 95% CI, 1.02-2.94]; P = .044) were independent predictors for the presence of LIPV triggers or drivers. A risk score model was established to predict the probability of LIPV triggers or drivers with persistent AF (10.2%), Dao-LIPV distance ≤2.5 mm (11.4%), and both (15.0%).

    CONCLUSION: The proximity of the Dao-LIPV was correlated to the presence of LIPV triggers or drivers. We developed a risk score model indicating that persistent AF and Dao-LIPV distances ≤2.5 mm significantly increase the risk of LIPV triggers or drivers, aiding electrophysiologists in preparing for and performing catheter ablation more effectively.

    MeSH terms: Aged; Female; Follow-Up Studies; Humans; Male; Middle Aged; Retrospective Studies; Risk Factors; Risk Assessment/methods
  13. Singh V, Shirbhate E, Kore R, Vishwakarma S, Parveen S, Veerasamy R, et al.
    Mini Rev Med Chem, 2025;25(1):76-93.
    PMID: 38982701 DOI: 10.2174/0113895575320344240625080555
    Globally, one of the most prevalent cancers is colorectal cancer (CRC). Chemotherapy and surgery are two common conventional CRC therapies that are frequently ineffective and have serious adverse effects. Thus, there is a need for complementary and different therapeutic approaches. The use of microbial metabolites to trigger epigenetic alterations as a way of preventing CRC is one newly emerging field of inquiry. Small chemicals called microbial metabolites, which are made by microbes and capable of altering host cell behaviour, are created. Recent research has demonstrated that these metabolites can lead to epigenetic modifications such as histone modifications, DNA methylation, and non-coding RNA regulation, which can control gene expression and affect cellular behaviour. This review highlights the current knowledge on the epigenetic modification for cancer treatment, immunomodulatory and anti-carcinogenic attributes of microbial metabolites, gut epigenetic targeting system, and the role of dietary fibre and gut microbiota in cancer treatment. It also focuses on short-chain fatty acids, especially butyrates (which are generated by microbes), and their cancer treatment perspective, challenges, and limitations, as well as state-of-the-art research on microbial metabolites-induced epigenetic changes for CRC inhibition. In conclusion, the present work highlights the potential of microbial metabolites-induced epigenetic modifications as a novel therapeutic strategy for CRC suppression and guides future research directions in this dynamic field.
    MeSH terms: Animals; Antineoplastic Agents/pharmacology; Antineoplastic Agents/chemistry; Humans
  14. Sultan OS, Sidhu P, Rehman K, Madheswaran T, Davamani AF
    Eur J Dent, 2025 Feb;19(1):103-109.
    PMID: 38977007 DOI: 10.1055/s-0044-1786863
    OBJECTIVE(S):  This study compared the antimicrobial efficacy of nanographene (NG) particles with chlorhexidine (CHX) and calcium hydroxide (Ca(OH)2) against Enterococcus faecalis.

    MATERIALS AND METHODS:  Forty extracted human mandibular premolar teeth were cleaned using a scaler, and the middle-third of the root (6 mm) was decoronated using a rotary diamond disk. The inner diameter of the teeth was made consistent using Gates Glidden Drills #3, treated with ethylene diamine tetra-acetic acid and sodium hypochlorite before sterilization. The samples were then contaminated with E. faecalis grown in Tryptic soy broth for 21 days. Tooth samples were then randomly divided into four groups: Group I (Control), untreated saline; Group II, Ca(OH)2; Group III, CHX; and Group IV, NG. The assessment of bacterial growth was carried out by harvesting dentin chips at the end of 1, 3, and 7 days. The colonies were physically counted and tabulated after 24 hours from seeding. Statistical analysis of the collected data was performed with analysis of variance and Tukey's post hoc test using SPSS Version 20.0.

    RESULTS:  The contaminated dentine blocks irrigated with NG (0.5 µg) and CHX (0 ± 0; p 

  15. Wang Y, Yan F, Xu DQ, Liu M, Liu ZF, Tang YP
    J Ethnopharmacol, 2025 Jan 10;336:118522.
    PMID: 38971345 DOI: 10.1016/j.jep.2024.118522
    ETHNOPHARMACOLOGICAL RELEVANCE: Labisia pumila (Blume) Fern.-Vill, also known as Kacip Fatimah, is a traditional medicinal herb common throughout Southeast Asia. It is primarily used to facilitate childbirth and postpartum recovery in women. Additionally, it can also be used to treat dysentery, rheumatism, gonorrhea, and as an anti-flatulent.

    AIM OF THIS REVIEW: This article aims to provide a comprehensive review of the traditional uses, botany, cultivation, phytochemistry, pharmacological effects, practical applications, and potential uses of L. pumila (LP). Furthermore, we also explore the safety of this plant and its potential prospects for application.

    MATERIALS AND METHODS: The keywords "Labisia pumila," "Kacip Fatimah," and "Marantodes pumilum" were used to collect relevant information through electronic searches (including Elsevier, PubMed, Google Scholar, Baidu Scholar, CNKI, ScienceDirect, and Web of Science).

    RESULTS: This review summarizes 102 chemical components from different parts of the plant, including flavonoids, phenolic acids, saponins, and other chemical components. In addition, we also address the associated cultivation conditions, traditional uses, pharmacological effects and toxicity. A large number of reports indicate that LP has various pharmacological effects such as antioxidant, phytoestrogenic, anti-inflammtory, antimicrobial, anti-osteoporosis and anti-obesity properties. These results provide valuable references for future research on LP. In addition, LP is also a potential medicinal and edible plant, and is currently sold on the market as a dietary supplement.

    CONCLUSIONS: LP is a renowned traditional ethnic medicine with numerous pharmacological activities attributed to its bioactive components. Therefore, isolation and identification of the chemical components in LP can be a focus of our future research. Current studies have focused only on the effects of LP on estrogen deficiency-related diseases in women and bone diseases. There is no scientific evidence for other traditional uses. Therefore, it is important to further explore its pharmacological activities and fill the research gaps related to other traditional uses. Furthermore, research on its safety should be expanded to prepare clinical applications.

    MeSH terms: Animals; Humans; Phytotherapy; Plants, Medicinal/chemistry; Primulaceae/chemistry
  16. Vihal S, Pundir S, Rathore C, Ranjan Lal U, Gupta G, Kumar Singh S, et al.
    Curr Drug Deliv, 2025;22(1):80-91.
    PMID: 38956909 DOI: 10.2174/0115672018246645231019131748
    BACKGROUND: The therapeutic effect of NS oil in mild to moderate psoriasis is limited owing to low play load of thymoquinone (<15 %w/w), irritation, dripping, low viscosity and thus, less contact time on the lesions.

    AIMS: This study aimed at developing and characterizing the ethanolic vesicular hydrogel system of Nigella sativa (NS) oil (NS EV hydrogel) for the enhancement of anti-psoriatic activity.

    OBJECTIVE: The objective of this study was to develop NS EV hydrogel and evaluate its anti-psoriatic activity.

    METHODS: The identification and quantification of TQ content in different NS seed extracts and marketed oil were measured by an HPTLC method using n-hexane and ethyl acetate as solvent systems. Preparation of ethanolic vesicles (EVs) was performed by solvent injection method, while its antipsoriatic activity was evaluated employing an Imiquad (IMQ)-induced plaque psoriasis animal model.

    RESULTS: A compact HPTLC band was obtained for TQ at an Rf value of 0.651. The calibration plot was linear in the range of 1-10 μg/spot, and the correlation coefficient of 0.990 was indicative of good linear dependence of peak area on concentration. From the different NS sources, the high TQ content was obtained in the marketed cold press oil, i.e., 1.45±0.08 mg/ml. Out of various NS oilloaded EVs, the F6 formulation revealed the smallest particle size (278.1 nm), with log-normal size distribution (0.459) and adequate entrapment efficiency. A non-uniform shape was observed in the transmission electron microscopy. The viscosity of F6 formulation hydrogel was 32.34 (Pa·s), which exhibited plastic behavior. In vivo, efficacy studies demonstrated decreased inflammation of the epidermis and dermis and a marked decrease in the levels of IL-17 by NS EV hydrogel compared to plain NS oil and standard drugs (Betamethasone and Dr. JRK Psorolin Oil).

    CONCLUSION: It may be concluded from the findings that NS-loaded EV gel was as good as betamethasone cream but more efficacious than the other treatments.

    MeSH terms: Ethanol/chemistry; Animals; Gels; Male; Rheology*; Hydrogels/chemistry; Carum; Nigella sativa/chemistry; Mice
  17. Sze Chong AS, Ahmad M, Alias H, Iqbal Hussain R, Lateh A, Chan CMH
    J Psychosoc Oncol, 2025;43(1):73-87.
    PMID: 38873844 DOI: 10.1080/07347332.2024.2364670
    INTRODUCTION: Childhood cancer caregivers report psychological distress and unmet psychosocial needs, affecting outcomes for their children. An experimental study was carried out to measure the effectiveness of an intervention in addressing traumatic stress, depression and anxiety.

    METHODS: Caregivers (n = 59) of children with ALL were allocated to both groups (intervention, n = 29; TAU control, n = 30) via the SNOSE method. The intervention is a physical copy of a 2-week psychosocial self-help guidebook. Scores on the PCL-5, BDI and BAI were recorded at baseline, post-intervention and 1-month follow-up.

    RESULTS: There was a statistically significant difference in traumatic stress symptoms post intervention (F(1, 57) = 5.760, p = .020, np2 = 0.093) in favor of the intervention group. No statistical significance was found for its effect at one-month follow-up, overall depression and anxiety.

    CONCLUSION: A psychosocial module developed for caregivers of children with ALL was found to be effective in reducing symptoms of traumatic stress and potentially depression. However, the maintenance of its effectiveness and the effectiveness on anxiety requires further study.

    MeSH terms: Adolescent; Adult; Anxiety*; Child; Child, Preschool; Depression*; Female; Follow-Up Studies; Humans; Male; Middle Aged; Stress, Psychological
  18. Nyein PP, Petoumenos K, Borok M, Eriobu N, Kumarasamy N, Avihingsanon A, et al.
    Clin Infect Dis, 2025 Feb 05;80(1):160-163.
    PMID: 38721980 DOI: 10.1093/cid/ciae256
    In this randomized controlled study, individuals taking dolutegravir+darunavir/ritonavir had greater increases in systolic and diastolic blood pressure than those taking 2 nucleoside reverse-transcriptase inhibitors+darunavir/ritonavir at week-48. The difference remained significant after controlling for confounding factors, including weight gain.
    MeSH terms: Darunavir/administration & dosage; Darunavir/therapeutic use; Adult; Female; Heterocyclic Compounds, 3-Ring/adverse effects; Heterocyclic Compounds, 3-Ring/therapeutic use; Humans; Male; Middle Aged; Piperazines; Pyridones*; Anti-HIV Agents/administration & dosage; Anti-HIV Agents/therapeutic use; Anti-Retroviral Agents/therapeutic use
  19. Wafriy CI, Nor-Ashikin MNK, Kamsani YS, Muid SA, Sarbandi MS
    Biol Trace Elem Res, 2025 Jan;203(1):334-345.
    PMID: 38717673 DOI: 10.1007/s12011-024-04183-8
    The involvement of the immune oxidative stress response in the pathophysiology and pathogenesis of allergic asthma is well documented. However, reports on the role of iron homeostasis in allergic asthma is scarce. Therefore, this study aims to identify iron-related genes and proteins in mouse models of allergic asthma. Related articles were identified from SCOPUS and Web of Science databases. The article search was limited to publications in English, within a 10-year period (2014 - 2023, up to 16 August 2023) and original/research papers. All identified articles were screened for eligibility using the inclusion and exclusion criteria. All eligible articles were quality appraised prior to data extraction. Five studies were selected for data extraction. Based on the extracted data, three themes and seven subthemes related to iron homeostasis were identified. The type of samples and analytical methods used were also identified. In conclusion, our study elucidates that iron-related proteins are regulated in animal models of allergic asthma. However, the currently available data do not allow us to conclude whether the disease model resulted in iron accumulation or depletion. Therefore, further studies with other related markers should be conducted.
    MeSH terms: Animals; Disease Models, Animal*; Homeostasis*; Mice
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