• 1 Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, 201564Islamic Azad University201564, Najafabad, Iran
  • 2 Department of Materials Engineering, 48456Isfahan University of Technology, Isfahan, Iran
  • 3 Advanced Membrane Technology Research Center (AMTEC), 54702Universiti Teknologi Malaysia, Johor Bahru, Johor, Malaysia
  • 4 Advanced Manufacturing Research Group, Faculty of Mechanical Engineering, 54702Universiti Teknologi Malaysia, Johor Bahru, Johor, Malaysia
  • 5 Department of Chemical Engineering Materials Environment, Sapienza University of Rome, Roma, Italy
J Biomater Appl, 2022 Dec 01.
PMID: 36454961 DOI: 10.1177/08853282221140672


Porous structure, biocompatibility and biodegradability, large surface area, and drug-loading ability are some remarkable properties of zeolite structure, making it a great possible option for bone tissue engineering. Herein, we evaluated the potential application of the ZSM-5 scaffold encapsulated GEN with high porosity structure and significant antibacterial properties. The space holder process has been employed as a new fabrication method with interconnected pores and suitable mechanical properties. In this study, for the first time, ZSM-5 scaffolds with GEN drug-loading were fabricated with the space holder method. The results showed excellent open porosity in the range of 70-78% for different GEN concentrations and appropriate mechanical properties. Apatite formation on the scaffold surface was determined with Simulation body fluid (SBF), and a new bone-like apatite layer shaping on all samples confirmed the in vitro bioactivity of ZSM-5-GEN scaffolds. Also, antibacterial properties were investigated against both gram-positive and gram-negative bacteria. The incorporation of various amounts of GEN increased the inhibition zone from 24 to 28 (for E. coli) and 26 to 37 (for S. aureus). In the culture with MG63 cells, great cell viability and high cell proliferation after 7 days of culture were determined.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.