Fulltext

Field evaluation of a PfHRP-2/pLDH rapid diagnostic test and light microscopy for diagnosis and screening of falciparum malaria during the peak seasonal transmission in an endemic area in Yemen

Alareqi LM 1 , Mahdy MA 2 , Lau YL 3 , Fong MY 4 , Abdul-Ghani R 5 , Ali AA 6 Show all authors , Cheong FW 7 , Tawfek R 8 , Mahmud R 9

Affiliations 

  • 1 Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. landleena@yahoo.com
  • 2 Tropical Disease Research Center, University of Science and Technology, Sana'a, Yemen. alsharaby9@yahoo.com
  • 3 Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. yllau@ummc.edu.my
  • 4 Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. fongmy@um.edu.my
  • 5 Tropical Disease Research Center, University of Science and Technology, Sana'a, Yemen. rashadqb@yahoo.com
  • 6 Yemeni-Swedish Hospital, Ministry of Public Health and Population, Taiz, Yemen. arwaalinasser4@gmail.com
  • 7 Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. fwcheong18@hotmail.com
  • 8 Yemeni-Swedish Hospital, Ministry of Public Health and Population, Taiz, Yemen. rehab.tawfeq@yahoo.com
  • 9 Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. rohela@ummc.edu.my
Malar J, 2016 Jan 28;15:49.
PMID: 26821911 DOI: 10.1186/s12936-016-1103-2

Abstract

Malaria is a public health threat in Yemen, with 149,451 cases being reported in 2013. Of these, Plasmodium falciparum represents 99%. Prompt diagnosis by light microscopy (LM) and rapid diagnostic tests (RTDs) is a key element in the national strategy of malaria control. The heterogeneous epidemiology of malaria in the country necessitates the field evaluation of the current diagnostic strategies, especially RDTs. Thus, the present study aimed to evaluate LM and an RDT, combining both P. falciparum histidine-rich protein-2 (PfHRP-2) and Plasmodium lactate dehydrogenase (pLDH), for falciparum malaria diagnosis and survey in a malaria-endemic area during the transmission season against nested polymerase chain reaction (PCR) as the reference method.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications