Affiliations 

  • 1 Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia
  • 2 Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Functional Device Laboratory, Institute of Advanced Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • 3 Low Dimensional Materials Research Centre (LDMRC), Physics Department, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Biosens Bioelectron, 2017 Mar 15;89(Pt 1):673-680.
PMID: 26718548 DOI: 10.1016/j.bios.2015.12.030

Abstract

In this study, a disposable and simple electrochemical immunosensor was fabricated for the detection of carcinoembryonic antigen. In this method, silver nanoparticles (AgNPs) were mixed with reduced graphene oxide (rGO) to modify the surface of screen-printed carbon electrode (SPE). Initially, AgNPs-rGO modified-SPEs were fabricated by using simple electrochemical deposition method. Then the carcinoembryonic antigen (CEA) was immobilized between the primary antibody and horseradish peroxidase (HRP)-conjugated secondary antibody onto AgNPs-rGO modified-SPEs to fabricate a sandwich-type electrochemical immunosensor. The proposed method could detect the CEA with a linear range of 0.05-0.50µgmL-1 and a detection limit down to 0.035µgmL-1 as compared to its non-sandwich counterpart, which yielded a linear range of 0.05-0.40µgmL-1, with a detection limit of 0.042µgmL-1. The immunosensor showed good performance in the detection of carcinoembryonic antigen, exhibiting a simple, rapid and low-cost. The immunosensor showed a higher sensitivity than an enzymeless sensor.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.