Background: The transition from high school to a university is a significant milestone in every student’s life. Stress experienced by students can have a detrimental effect on their academic performance. Social support has generally been found to promote psychological well-being, as well as to buffer the effects of stress. Method: The study was conducted in one of the Universities in Malaysia. The study questionnaire was designed to include questions regarding socio-demographic aspects and stress of the students; in addition, the Multidimensional Scale of Perceived Social Support scale (MSPSS) that was developed by Zimet was administered. The main aim of this study is to investigate the relationship between Perceived Social Support, Stress and Gender differences among University Students. Results: In this study only a small group of the students scored very low on the Perceived social support scale. A low significant inverse correlation was found between MSPSS and stress. Male students’ experienced greater stress than female students’. Female students had a better perception of social support from their families compared to male students. Conclusion: The fact that, as the level of social support increase the level of stress decrease clearly shows that social support acts as a buffer to stress.