Bronchodilator effects of Lignosus rhinocerotis extract on rat isolated airways is linked to the blockage of calcium entry

Lee MK 1 , Lim KH 2 , Millns P 3 , Mohankumar SK 4 , Ng ST 5 , Tan CS 5 Show all authors , Then SM 1 , Mbaki Y 3 , Ting KN 6

Affiliations 

  • 1 Department of Biomedical Sciences, University of Nottingham Malaysia Campus, Semenyih 43500, Malaysia
  • 2 School of Pharmacy, University of Nottingham Malaysia Campus, Semenyih 43500, Malaysia
  • 3 School of Life Sciences, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK
  • 4 JSS College of Pharmacy, Rocklands, Ootacamund-643001, Tamil Nadu, India; a constituent college of JSS Academy of Higher Education & Research, Mysuru, Karnataka, India
  • 5 LiGNO Biotech Sdn Bhd, Balakong Jaya 43300, Malaysia
  • 6 Department of Biomedical Sciences, University of Nottingham Malaysia Campus, Semenyih 43500, Malaysia. Electronic address: Kang-Nee.Ting@nottingham.edu.my
Phytomedicine, 2018 Mar 15;42:172-179.
PMID: 29655683 DOI: 10.1016/j.phymed.2018.03.025

Abstract

BACKGROUND: Lignosus rhinocerotis (Cooke) Ryvarden is a popular medicinal mushroom used for centuries in Southeast Asia to treat asthma and chronic cough. The present study aimed to investigate the effect of this mushroom on airways patency.

MATERIALS AND METHODS: The composition of L. rhinocerotis TM02 cultivar was analyzed. Organ bath experiment was employed to study the bronchodilator effect of Lignosus rhinocerotis cold water extract (CWE) on rat isolated airways. Trachea and bronchus were removed from male Sprague-Dawley rats, cut into rings of 2 mm, pre-contracted with carbachol before adding CWE into the bath in increasing concentrations. To investigate the influence of incubation time, tissues were exposed to intervals of 5, 15 and 30 min between CWE concentrations after pre-contraction with carbachol in subsequent protocol. Next, tissues were pre-incubated with CWE before the addition of different contractile agents, carbachol and 5-hydroxytrptamine (5-HT). The bronchodilator effect of CWE was compared with salmeterol and ipratropium. In order to uncover the mechanism of action of CWE, the role of beta-adrenoceptor, potassium and calcium channels was investigated.

RESULTS: Composition analysis of TM02 cultivar revealed the presence of β-glucans and derivatives of adenosine. The extract fully relaxed the trachea at 3.75 mg/ml (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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