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Bioactive 2-(Methyldithio)Pyridine-3-Carbonitrile from Persian Shallot (Allium stipitatum Regel.) Exerts Broad-Spectrum Antimicrobial Activity

Karunanidhi A 1 , Ghaznavi-Rad E 2 , Jeevajothi Nathan J 3 , Joseph N 4 , Chigurupati S 5 , Mohd Fauzi F 6 Show all authors , Pichika MR 7 , Hamat RA 8 , Lung LTT 9 , van Belkum A 10 , Neela V 11

Affiliations 

  • 1 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. arunmscimb@gmail.com
  • 2 Department of Microbiology and Immunology, Faculty of Medicine, Arak University of Medical Sciences, Basij Square, Arak 38481-7-6941, Iran. ghaznaviehs@yahoo.com
  • 3 School of Medicine, Taylor's University, Lakeside Campus, No. 1, Jalan Taylor's, Subang Jaya 47500, Selangor Darul Ehsan, Malaysia. jjayakayatri@yahoo.com
  • 4 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. narcissemsjoseph@gmail.com
  • 5 Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraidah 52571, Kingdom of Saudi Arabia. sridevi.phd@gmail.com
  • 6 Department of Pharmacology and Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA, Bandar Puncak Alam 42300, Selangor Darul Ehsan, Malaysia. fazlin205@gmail.com
  • 7 Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, 126 Jalan Bukit Jalil, Kuala Lumpur 57000, Malaysia. mallikarjunarao_pichika@imu.edu.my
  • 8 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. rukman@upm.edu.my
  • 9 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. leslie@upm.edu.my
  • 10 R & D Microbiology, BioMerieux, 3 route de Port Michaud, 38390 La Balme-les-Grottes, France. alex.vanbelkum@biomerieux.com
  • 11 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. vasantha@upm.edu.my
Molecules, 2019 Mar 13;24(6).
PMID: 30871159 DOI: 10.3390/molecules24061003

Abstract

Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 µg·mL-1) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 µg·mL-1 for tested bacterial species and 0.25 to 2 µg·mL-1 for Candida spp. Further studies are important to confirm the drug target and mechanism of action.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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