Affiliations 

  • 1 School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia
  • 2 Laboratory for Microbiome Sciences, RIKEN Center for Integrative Medical Sciences, Kanagawa, 230-0045, Japan; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia
  • 3 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia
  • 4 School of Data Sciences, Perdana University, 43400, Selangor, Malaysia
  • 5 Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Kanagawa, 230-0045, Japan; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia. Electronic address: hiroshi.ohno@riken.jp
  • 6 School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia. Electronic address: mintze.liong@usm.my
J Biotechnol, 2019 Jul 20;300:20-31.
PMID: 31095980 DOI: 10.1016/j.jbiotec.2019.05.006

Abstract

Increasing levels of antibiotic resistance in pathogens, including Staphylococcus aureus, remains a serious problem for public health, leading to the need for better alternative antimicrobial strategies. The antimicrobial proteins produced by Lactobacillus plantarum USM8613 attributed to its anti-staphylococcal activity were identified as extracellular transglycosylase and glyceraldehyde-3-phosphate dehydrogenase (GADPH), both with different mechanisms of action. Extracellular transglycosylase, which contains a LysM domain, exerts a cell wall-mediated killing mechanism, while GADPH penetrates into S. aureus cells and subsequently induces the overexpression of autolysis regulators, resulting in S. aureus autolysis. Both extracellular transglycosylase and GADPH exert anti-inflammatory effects in S. aureus-infected HaCaT cells by reducing the expression and production of TLR-2, hBDs and various pro-inflammatory cytokines (IL-1α, IL-1β, IL-6, TNF-α, and IL-8). Taken together, extracellular transglycosylase and GADPH produced by L. plantarum USM8613 could potentially be applied as an alternative therapeutic agent to treat S. aureus skin infections and promote skin health.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.